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鸡Apo VLDLⅡ基因内含子Ⅰ的多态性研究

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畜牧与兽医 2007年第8期39卷 8-11页

作者：程金花 hassan hussein musa 鞠慧萍许盛海黄军陈国宏扬州大学动物科学与技术学院江苏扬州225009

本研究根据鸡Apo VLDL-Ⅱ基因内含子Ⅰ的基因序列设计3对引物,分别采用PCR-RFLP、PCR-SSCP技术对文昌鸡、如皋黄鸡、丝羽乌骨鸡、安卡肉鸡及红色原鸡5个鸡种进行多态性研究。结果表明,鸡Apo VLDL-Ⅱ基因内含子Ⅰ存在4处突变,分别为:1950... 详细信息

本研究根据鸡Apo VLDL-Ⅱ基因内含子Ⅰ的基因序列设计3对引物,分别采用PCR-RFLP、PCR-SSCP技术对文昌鸡、如皋黄鸡、丝羽乌骨鸡、安卡肉鸡及红色原鸡5个鸡种进行多态性研究。结果表明,鸡Apo VLDL-Ⅱ基因内含子Ⅰ存在4处突变,分别为:1950处G/A、2522处A/C、2705处A/C及2793处C/T。统计结果显示:安卡肉鸡基因型频率的分布与如皋黄鸡、红色原鸡存在显著差异,文昌鸡基因型频率的分布与乌骨鸡差异不显著,除安卡肉鸡外所有鸡种均处于Hardy-Weinberg平衡状态。

关键词：鸡 RFLP SSCP Apo VLDLⅡ 多态性

牛体外受精胚胎衍生干细胞能力影响因素的研究

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畜牧兽医学报 2006年第9期37卷 928-932页

作者：李碧春陈国宏吴信生徐琪吴圣龙蔡中涛 hassan hussein musa 扬州大学动物科学与技术学院扬州225009

以牛卵巢卵母细胞体外受精获取囊胚期胚胎，比较体外受精牛胚胎不同获取内细胞团的方法和不同培养液对体外培养胚胎干细胞能力的影响。结果表明，囊胚期胚胎不除去透明带而直接培养产生胚胎干细胞，初次克隆率为55％；胰酶法去透明带分... 详细信息

以牛卵巢卵母细胞体外受精获取囊胚期胚胎，比较体外受精牛胚胎不同获取内细胞团的方法和不同培养液对体外培养胚胎干细胞能力的影响。结果表明，囊胚期胚胎不除去透明带而直接培养产生胚胎干细胞，初次克隆率为55％；胰酶法去透明带分离的内细胞团（ICM）培养产生胚胎干细胞，初次克隆率为90％。免疫外科法去透明带分离的ICM在4种不同的培养液中，初次克隆率分别为16．4％、11．5％、21．8％、18．2％，但是其分离的ICM经传代后形成的衍生细胞不易发生分化，经过4～6代的传代，仍然保持其完整的形态，说明免疫外科法是一种理想的牛ICM分离法，培养液为D20＋LIF（40ng／mL）可用于牛胚胎干细胞培养。

关键词：牛卵母细胞体外受精胚胎干细胞

禽致病性大肠杆菌FimH黏附素致病性作用初探

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禽致病性大肠杆菌FimH黏附素致病性作用初探

Microsatellite-Based Genetic Differentiation and Phylogeny of Sheep Breeds in Mongolia Sheep Group of China

中国畜牧兽医学会禽病学分会第十四次学术研讨会

作者： hassan hussein musa 朱国强扬州大学兽医学院 Faculty of Veterinary Science University of NyalaNyala 155Sudan

1.前言:禽致病性大肠杆菌(APEC)Ⅰ型菌毛与其易感细胞表面大分子受体相互作用,在不同宿主组织细胞定植中起着关键性作用,而FimH黏附素是Ⅰ型菌毛特异性黏附于易感细胞表面甘露糖受体所必须的。本研究的目的是检测FimH黏附素在体外黏附...

1.前言:禽致病性大肠杆菌(APEC)Ⅰ型菌毛与其易感细胞表面大分子受体相互作用,在不同宿主组织细胞定植中起着关键性作用,而FimH黏附素是Ⅰ型菌毛特异性黏附于易感细胞表面甘露糖受体所必须的。本研究的目的是检测FimH黏附素在体外黏附细胞系中的作用。

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Agricultural Sciences in China 2011年第7期10卷 1080-1087页

作者： SUN Wei CHANG Hong musa hussein hassan LIAO Xin-jun CHU Ming-xing Kija James Animal Science and Technology College Yangzhou University Yangzhou 225009 P.R.China Faculty of Veterinary Science University of Nyala Nyala 155 Sudan Key Laboratory of Farm Animal Genetic Resources and Utilization Ministry of Agriculture/Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing 100193 P.R.China Queensland Bioscience Precinct 306 Carmody Road St Lucia QLD 40723 Australia

In the present study we studied the genetic structure of five Chinese sheep populations of Hu sheep, Tong sheep, Small-tailed Han sheep, Tan sheep, and Wadi （WD） sheep using 15 microsatellite loci. The results showed that the FIT, FST, and FIS statistics computed for the complete dataset had the following values, 0.523±0.140, 0.363±0.131 and 0.263±0.092, respectively. All loci were significantly contributed to the genetic differentiation among population （P0.001）. There is no relationship between the scatter of pairwise FST geographical distance points as geographical distance increases between the five populations. Membership probabilities and genetic structure of sheep populations were estimated when K=2, the populations were classified into Hu, Tong, Han and WD, and Tan sheep group. However, when K=3, the populations were classified into Hu and Tong, Han and WD, and Tan sheep group. The findings supported the previous literatures that these populations are originated on different time stages from the primogenitor population and communicated genetically with each other by natural and artificial selection in different ecological environment.

关键词： genetic differentiation genetic phylogeny microsatellite DNA Mongolian sheep

Study on genetic coadaptability of wild quail populations in China

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Science China(Life Sciences) 2006年第2期49卷 172-181页

作者： hassan hussein Animal Science & Technology College Yangzhou University

Genetic coadaptability of wild Japanese quail, wild Common quail and Domestic quail populations in China was studied using 7 microsatellite DNA markers and Monte Carlo method to test genetic disequilibrium. The molecular effects of genetic coadaptability were analyzed through a new statistical model of neutral site. The results showed that genetic coadaptability dominated the genetic disequilibrium of the three quail populations, and totally 16.67%, 9.66% and 10.05% of non-allelic combinations were in the genetic disequilibrium in wild Japanese quail, wild Common quail and Do-mestic quail populations, respectively. Genetic coadaptability existed at almost all the tested sites. In the molecular point of view, genetic coadaptability plays an important role of keeping lots of poly-morphisms in natural populations. Therefore, it is another key factor to the genetic disequilibrium in the population except for linkage. The results enrich the conceptions and connotations of genetic disequilibrium, and help us know more about genetic coadaptability and its effects, and lay a founda-tion of evaluation and protection of wild quail genetic resources in China.

关键词： microsatellite, wild quail, genetic coadaptability, linkage, genetic disequilibrium.

Rapid conversion of cellulose to 5-hydroxymethylfurfural using single and combined metal chloride catalysts in ionic liquid

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燃料化学学报 2013年第2期41卷 214-222页

作者： hussein Abou-Yousef El Barbary hassan Philip Steele Department of Forest Products Mississippi State University Cellulose & Paper Department National Research CenterDokkiCairoEgypt

Direct conversion of cellulose into 5-hydroxymethylfurfural(HMF) was performed by using single or combined metal chloride catalysts in 1-ethyl-3-methylimidazolium chloride(Cl) ionic *** study demonstrated formation of 2-furyl hydroxymethyl ketone(FHMK),and furfural(FF) simultaneously with the formation of *** reaction parameters were addressed to optimize yields of furan derivatives produced from cellulose by varying reaction temperature,time,and the type of metal chloride *** reaction by using FeCl3 resulted in 59.9% total yield of furan derivatives(HMF,FHMK,and FF) from ***3 was the most effective catalyst for selective conversion of cellulose into HMF(35.6%) with less concentrations of FHMK,and *** the yields of furans produced from cellulose could be achieved via reactions catalyzed by different combinations of two metal *** optimization was carried out to produce total furans yield 75.9% by using FeCl3/CuCl2 ***3/CuCl2 was the most selective combination to convert cellulose into HMF(39.9%) with total yield(63.8%) of furans produced from the *** temperature and time of the catalytic reaction played an important role in cellulose conversion,and the yields of *** the reaction temperature could enhance the cellulose conversion and HMF yield for short reaction time intervals(5~20 min).

关键词：化学动力学燃料化学理论 EMIM HMF

Incorporation of silica into the goethite structure: a microscopic and spectroscopic study

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Acta Geochimica 2018年第6期37卷 911-921页

作者： Abdullah musa Ali Eswaran Padmanabhan hassan Baioumy Department of Geosciences Faculty of Geosciences and Petroleum Engineering Universiti Teknologi PETRONAS(UTP)

Quartz and iron(hydr)oxide are reactive surface phases that are often associated with one another in soils and sediments. Despite the several studies on the coating of quartz with iron oxides, the reactivity of dissolved species(Si) leached from quartz with iron(hydr)oxides has received limited attention. In this study, goethite synthesized on quartz substrates were characterized using field emission scanning electron microscopy, X-ray diffraction(XRD), transmission electron microscopy, and Fouriertransform infrared(FT-IR) spectroscopy. The SEM characterization revealed that bundles of thin parallel aligned goethite rods were formed at p H [ 10, while large pseudohexagonal crystals of twinned goethite needles were synthesized at p H B 10 after dehydration and hydration in the alkaline media. TEM analysis showed expanded and distorted lattice spacing of the crystal structure of iron(hydr)oxide due to silica incorporation. The characterization showed that silica increased the crystallite size of the goethite and transformed its acicular texture to a larger,twinned needle structure. FT-IR and XRD analyses revealed band shifts in crystal bonds as well as new bond formations, which indicate the presence of changes in the chemical environment of Fe–O and Si–O bonds. Thus, the presence of sorbed silicates modifies the crystal and lattice structure of goethite.

关键词： Quartz Goethite Twinned goethite Microscopic characterization(FESEM and TEM) FT-IR spectroscopy

Role of pancreatic juice cytology in diagnosis of high-grade pancreatic intraepithelial neoplasia

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World Journal of Clinical Cases 2025年第10期13卷 59-61页

作者： hussein hassan Okasha Mohammed Tag-Adeen Hossam Eldin Shaaban Division of Gastroenterology and Hepatology Department of Internal MedicineKasr Al-Aini School of MedicineCairo UniversityCairo 11562Egypt Division of Gastroenterology and Hepatology Department of Internal MedicineQena Faculty of MedicineSouth Valley UniversityQena 83523Egypt Department of Internal Medicine and Gastroenterology National Hepatology and Tropical Medicine Research InstituteCairo 11796Egypt

High-grade pancreatic intraepithelial neoplasia is a challenging diagnosis and itdoes not exhibit mass lesions. It is suspected based on changes in the mainpancreatic duct in magnetic resonance cholangiopancreatography. Sometimesonly an unclear duct shows in magnetic resonance cholangiopancreatographywith no focal strictures and upstream dilatation of the main pancreatic duct. Serialpancreatic juice cytology is valuable in diagnosis of those patients.

关键词： High-grade pancreatic intraepithelial neoplasia Pancreatic cancer Magnetic resonance cholangiopancreatography Endoscopic retrograde pancreatography Pancreatic juice cytology Pancreatic ductal adenocarcinoma

Faulty Phase Identification for Transmission Line with Metal Oxide Varistor-protected Series Compensator

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Journal of Modern Power Systems and Clean Energy 2021年第1期9卷 85-93页

作者： Mohammed Hussien hassan musa Ling Fu Zhengyou He Yumin Lei the Electrical Engineering Department Southwest Jiaotong UniversityChengduChina the Electrical Engineering Department College of EngineeringAlmughtarbeen UniversityKhartoumSudan

The nonlinear operation of metal oxide varistor(MOV)-protected series compensator in transmission lines introduces complications into fault detection approaches. The accuracy of a conventional fault detection schemes is adversely affected by continuous change of the system impedance and load current at the point of a series compensation unit. Thus, this study suggests a method for detecting the faulted phase in MOV-protected series-compensated transmission lines. Primarily, the fault feature is identified using the covariance coefficients of the current samples during the fault period and the current samples during the pre-fault period. Furthermore, a convenience fault detection index is established by applying the cumulative sum technique. Extensive validation through different fault circumstances is accomplished, including different fault positions,resistances, and inception times. The experimental results show that the proposed method performs well with high resistance or impedance faults, faults in noisy conditions, and close-in and far-end faults. The proposed method is simple and efficient for faulty phase detection in MOV-protected series-compensated transmission lines.

关键词： Cumulative approach covariance coefficient high-impedance fault response time