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Development of a Total Flavonoids Extract of Artemisia Rupestris L.via Nanotechnology and Its Antiviral Effect In Vitro

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Infectious Diseases Research 2020年第2期1卷 5-14页

作者： hasimu hamulati Su-Xiang Chen Zhen-Yi Gu Yu-Hua Sun Tao Wang The Xingjian Institute of Materia Medica Xinjiang Uygur Autonomous RegionUrumqiP.R.China830004 Centre for Molecular Medicine and Innovative Therapeutics Murdoch UniversityPerth 6150Australia The College of Nursing and Health Zhengzhou UniversityZhengzhou450001China

Objective:We aimed to develop a novel Artemisia rupestris L.flavonoid nano-encapsulation(AFN)preparation and evaulate its anti-hepatitis B virus(HBV)activityin vitro.Methods:First,the AFN was prepared using polylactic-co-glycolic acid(PLGA).Then,after verification of the AFN,in vitro anti-virus assays were conducted by:(1)assessing the inhibitory effect of AFN on the secretion of hepatitis B surface antigens(HBsAg),hepatitis B e-antigens(HBeAg),and the replication of HBV DNAin HepG2.2.15 cells;(2)analyzing the influence of AFN on the activation rate of NF-κB positive cells;and(3)evaluating the effect of AFN on the function of glutathione peroxidase(GSH-PX)enzymes located onthe HepG2.2.15 cell membrane.Results:Compared to the original total flavonoids extract of Artemisia rupestris L.(withoutnano-encapsulation),AFN preparation under the maximum nontoxic concentration effectively inhibited the secretion of HBsAg,HBeAg,and HBV DNA from HepG2.2.15 cells.At the same time,AFN preparation promoted not only the activation rate of NF-κB positive cells,but also antiviral GSH-PX enzyme function.In conclusion,nano-encapsulation of the flavonoids extract of Artemisia rupestris L.showed an enhanced anti-HBV effect in vitro compared to the original total flavonoids extract(without nanoencapsulation);therefore,nano-encapsulation has great potential for the development of a novel antiviral herbal medicine preparation with improved efficacy.

关键词： Artemisia rupestris L. HBV PLGA Nanoparticle Traditional medicine

Protection of Citrullus colocynthis Fruit Extracts on Carbon Tetrachloride-induced and Bacillus Calmette-Guerin plus Lipopolysaccharide-induced Hepatotoxicity in Mice

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Chinese Herbal Medicines 2013年第3期5卷 205-211页

作者： YANG Wei-jun GU Zheng-yi TURSUN Dilnur CHEN Bo hasimu hamulati LIU Chong HE Jiang XU Lei MAO Yan Xinjiang Key Laboratory of Xinjiang Uigur Medicine Xinjiang Institute of Materia Medica

Objective To investigate the hepatoprotective activities of the extracts from Citrullus colocynthis(ECC),a native plant used as traditional Uigur Medicine on acute liver injury in mice.Methods The activities of ECC of petroleum ether(ECCPE),chloroform(ECCC),ethyl acetate(ECCEA),n-butyl alcohol(ECCBA),and water(ECCW) were evaluated in vivo using two experimental models,carbon tetrachloride(CCl4)- and bacillus calmette-guerin(BCG) plus lipopolysaccharide(LPS)-induced acute hepatotoxicity in mice.The contents of aspartate aminotransferase(AST) and alanine aminotransferase(ALT) in serum were determined and the liver histological examination was carried out,respectively.Results The pretreatment with ECC for 7 d obviously reduced the impact of CCl4toxicity on the serum markers of liver damage,ECCEA and ECCC with a significant difference of AST(P < 0.01,0.05,respectively) and ALT(P < 0.05,0.01,respectively).The protective activity was reconfirmed against BCG + LPS-induced injury and the serum enzymatic levels were obviously elevated,for ECCEA and ECCC with a significant difference of AST(P < 0.05,0.01,respectively) and ALT(P < 0.01,0.05,respectively).Conclusion That ECCEA and ECCC are the potent hepatoprotective extracts that could protect liver against the acute injury,and this ability might be attributed to their hepatoprotective potentials.

关键词： bacillus calmette-guerin plus lipopolysaccharide carbon tetrachloride Citrullus colocynthis hepatoprotective activities Uigur Medicine

CD147通过Akt/mTOR信号通路调控脂肪酸合成对子宫颈癌细胞增殖、侵袭和迁移的影响

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临床与实验病理学杂志 2024年第3期40卷 261-267页

作者：李金秋尚香玉严怡然艾丽努尔·艾尼阿仙姑·哈斯木新疆医科大学基础医学院/新疆地方病分子生物学重点实验室乌鲁木齐836001

目的探讨CD147的表达对子宫颈癌细胞增殖、侵袭、迁移的影响和潜在的分子机制。方法分析UCSC数据库BSG基因(编码CD147蛋白)在子宫颈癌中的表达,采用Log-rank test法评估各组表达的预后差异。应用Western blot法检测CD147在Siha、Hela和H... 详细信息

目的探讨CD147的表达对子宫颈癌细胞增殖、侵袭、迁移的影响和潜在的分子机制。方法分析UCSC数据库BSG基因(编码CD147蛋白)在子宫颈癌中的表达,采用Log-rank test法评估各组表达的预后差异。应用Western blot法检测CD147在Siha、Hela和H8细胞中的表达,通过慢病毒转染Hela细胞下调CD147表达,并验证其转染效率。运用Western blot法检测各组细胞中p-Akt、p-mTOR、ACC1、FASN、E-cadherin和N-cadherin的表达;使用BODIPY染色和脂肪酸试剂盒检测细胞中脂肪酸含量;利用CCK-8、平板克隆和Transwell实验检测细胞增殖、侵袭和迁移能力。sh-CD147组经Akt激动剂(SC79)处理后,Western blot法检测细胞中p-Akt、p-mTOR、ACC1、FASN、E-cadherin和N-cadherin的表达;选用BODIPY染色和脂肪酸试剂盒检测细胞中脂肪酸含量;利用平板克隆实验和Transwell实验分别检测细胞增殖、侵袭和迁移能力。结果UCSC数据库显示CD147在子宫颈癌中的表达高于正常子宫颈组织(P<0.01);CD147过表达的患者预后不良。Western blot检测结果显示,与H8细胞相比,Siha和Hela细胞中CD147蛋白表达增高(P=0.011)。下调CD147表达后,与Hela组相比,sh-CD147组细胞中CD147、ACC1和FASN蛋白表达降低(P<0.001);BODIPY荧光染色减弱,脂肪酸含量下降(P<0.001);细胞集落形成能力、侵袭和迁移能力降低;sh-CD147组细胞中E-cadherin蛋白表达升高,N-cadherin、p-Akt和p-mTOR表达降低。与sh-CD147相比,经Akt激动剂SC-79剂处理后(sh-CD147-SC79)细胞内p-Akt、p-mTOR、ACC1、FASN和N-cadherin表达升高,E-cadherin表达降低,脂质染色和脂肪酸含量检测结果与关键酶表达一致(P<0.01),细胞增殖、侵袭迁移能力显著增强。结论CD147通过Akt/mTOR信号通路调控脂肪酸合成,促进子宫颈癌细胞增殖、侵袭和迁移。

关键词：子宫颈肿瘤脂肪酸合成增殖侵袭迁移 CD147

Quercetin protects human brain microvascular endothelial cells from fibrillarβ-amyloid_(1–40)-induced toxicity

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Acta Pharmaceutica Sinica B 2015年第1期5卷 47-54页

作者： Yongjie Li Sibai Zhou Jinze Li Yuhua Sun Hamlati hasimu Rui Liu Tiantai Zhang Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Xinjiang Key Laboratory of Xinjiang Uygur Medicine Xinjiang Institute of Materia Medica

Amyloid beta-peptides(Aβ) are known to undergo active transport across the blood-brain barrier, and cerebral amyloid angiopathy has been shown to be a prominent feature in the majority of Alzheimer's disease. Quercetin is a natural flavonoid molecule and has been demonstrated to have potent neuroprotective effects, but its protective effect on endothelial cells under Aβ-damaged condition is unclear. In the present study, the protective effects of quercetin on brain microvascular endothelial cells injured by fibrillar Aβ_(1–40)(f Aβ_(1–40)) were observed. The results show that f Aβ_(1–40)-induced cytotoxicity in human brain microvascular endothelial cells(h BMECs) can be relieved by quercetin treatment. Quercetin increases cell viability, reduces the release of lactate dehydrogenase, and relieves nuclear condensation.Quercetin also alleviates intracellular reactive oxygen species generation and increases superoxide dismutase activity. Moreover, it strengthens the barrier integrity through the preservation of the transendothelial electrical resistance value, the relief of aggravated permeability, and the increase of characteristic enzyme levels after being exposed to f Aβ_(1–40). In conclusion, quercetin protects h BMECs from f Aβ_(1–40)-induced toxicity.

关键词： Alzheimer’s disease Fibrillar Aβ1–40 Human brain microvascular endothelial cells Quercetin

小粒径载药微球联合信迪利单抗及贝伐珠单抗治疗不可切除肝癌的安全性及疗效分析

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中华介入放射学电子杂志 2024年第1期12卷 45-50页

作者：曹耿飞任伟新张海潇顾俊鹏阿斯哈尔·哈斯木新疆医科大学第一附属医院介入放射科新疆乌鲁木齐830054

目的探讨小粒径载药微球(70~150μm)栓塞联合信迪利单抗及贝伐珠单抗治疗不可切除中晚期原发性肝癌的安全性及疗效。方法回顾性分析2021年4月至2022年10月新疆医科大学第一附属医院采用小粒径载药微球联合信迪利单抗及贝伐珠单抗治疗的3... 详细信息

目的探讨小粒径载药微球(70~150μm)栓塞联合信迪利单抗及贝伐珠单抗治疗不可切除中晚期原发性肝癌的安全性及疗效。方法回顾性分析2021年4月至2022年10月新疆医科大学第一附属医院采用小粒径载药微球联合信迪利单抗及贝伐珠单抗治疗的34例不可切除中晚期肝癌患者临床资料。根据改良版实体瘤疗效评价标准(modified response evaluation criteria in solid tumors,mRECIST)评价标准评估肿瘤反应,观察小粒径载药微球联合信迪利单抗及贝伐珠单抗的不良反应情况,同时分析患者无疾病进展生存期、客观缓解率等情况。结果术后所有患者均经历了Ⅰ~Ⅲ级不同程度的不良反应,以术后疼痛最为显著;无Ⅴ级以上及致死性不良事件发生。本组随访时间3~18个月,中位随访时间15个月。中位无疾病进展期为15个月。术后1、3、6、12个月的客观缓解率分别为70.6%、76.5%、61.8%、50.0%;疾病控制率分别为100%、94.1%、82.4%、67.7%。结论小粒径载药微球联合信迪利单抗及贝伐珠单抗治疗不可切除中晚期肝癌显示出良好的安全性、耐受性,同时具有较长的无疾病进展生存期及更高的客观缓解率。

关键词：小粒径载药微球信迪利单抗贝伐珠单抗肝细胞肝癌

二茬次罗布白麻和罗布红麻叶不同采收期精油成分的GC-MS分析

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中国麻业科学 2024年第3期46卷 175-183页

作者：吴倩马浩隗亚军贾永倩艾力克木·哈斯木刘松岩李沁馨麻浩新疆农业大学草业学院新疆乌鲁木齐830052 新疆尉犁县林业和草原局新疆尉犁县841500 罗布麻产业发展服务中心新疆尉犁县841500 新疆生产建设兵团第二中学新疆乌鲁木齐830000 南京农业大学作物遗传与种质创新利用全国重点实验室江苏南京210095

以新疆的两种罗布麻为原材料,采用有机溶剂提取法并结合气相色谱-质谱联用仪(GC-MS),比较和分析二茬次罗布白麻和罗布红麻叶不同采收期的精油含量与化学成分的差异,采用峰面积归一法确定其成分种类及各成分的相对含量。结果表明:二茬次... 详细信息

以新疆的两种罗布麻为原材料,采用有机溶剂提取法并结合气相色谱-质谱联用仪(GC-MS),比较和分析二茬次罗布白麻和罗布红麻叶不同采收期的精油含量与化学成分的差异,采用峰面积归一法确定其成分种类及各成分的相对含量。结果表明:二茬次罗布红麻叶精油含量显著高于二茬次罗布白麻叶精油,不同采收期的罗布白麻和罗布红麻叶精油提取率、成分种类、成分数量存在较大差异,其中10月的二茬次罗布红麻叶精油含量最高。两种二茬次罗布麻叶及不同采收期精油均检测出含量较高的叶绿醇(Phytol)、新植二烯(Neophytadiene)、植物醇(3,7,11,15-Tetramethyl-2-hexadecen-1-ol)、2,4-二叔丁基苯酚(2,4-Di-tert-butylphenol)、正十六烷酸(n-Hexadecanoic acid)等。研究认为,二茬次罗布红麻叶适合药用,罗布白麻更适用于化妆品行业,且二茬次罗布红麻叶最佳采收期为9月,二茬次罗布白麻叶最佳采收期为10月。

关键词：罗布白麻罗布红麻气相色谱-质谱联用仪化学成分

Expressions of Toll-like receptors 3,4,7,and 9 in cervical lesions and their correlation with HPV16 infection in Uighur women

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Chinese Journal of Cancer 2011年第5期30卷 344-350页

作者： Ayshamgul hasimu Lin Ge Qiao-Zhi Li Rui-Ping Zhang Xia Guo Department of Pathology Xinjiang Medical University Urumqi Xinjiang 830011 P. R. China Department of Gynecology Wujiaqu Hospital Nongtiushi Xinjiang 831300 P. R. China Key Laboratory of Endemic Diseases Xinjiang Medical University Urumqi Xinjiang 830011 P. R. China

Recent findings show that Toll-like receptors(TLRs) expressed in immune cells play a crucial role in the innate immune response and the subsequent induction of adaptive immune responses against microbial infection on tissue injury.Furthermore,expression of TLRs in cancer cells is associated with tumor proliferation and invasion.To explore the role of TLRs expression in cervical carcinogenesis in Uighur women,we detected the expressions of TLR3,TLR4,TLR7,and TLR9 in 25 normal cervical tissues,64 cervical intraepithelial neoplasia(CIN) tissues,and 63 cervical squamous cell carcinoma(CSCC) tissues using immunohistochemical staining,as well as human papillomavirus type 16(HPV16) infection using PCR.All samples used in this study were from Xinjiang Uighur women.We found the expression levels of TLR4,TLR7,and TLR9 were significantly higher in CIN and CSCC than in normal controls(P 0.05).Up-regulation of TLR9 was correlated with lymph node metastasis(P 0.05).We also analyzed the correlation between the expressions of TLRs and HPV16 infection and found that the expressions of TLR4 and TLR9 significantly correlated with HPV16 infection in CIN(r = 7.434,P = 0.006;r = 7.123,P = 0.008) and CSCC(r = 6.423,P = 0.001;r = 8.478,P = 0.004),whereas the expression of TLR3 was not significantly different in any of the three groups and had no significant correlation with HPV16 infection.Our results suggest that high expression of TLR4,TLR7,and TLR9 may play important roles in the development and progression of CIN and CSCC in Uighur women,and the expressions of TLR4 and TLR9 can be up-regulated by HPV16 infection.

关键词： Toll样受体微生物感染 HPV16 维吾尔族宫颈癌妇女免疫组织化学染色 TLR9

基于肠道微生物学及代谢组学研究2型糖尿病患者肠道菌群结构及血清代谢产物的特征

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广东医学 2024年第2期45卷 139-144页

作者：阿孜古丽·买合买提米日古丽·吾木哈斯木李雪梅塞娜瓦尔·阿西木穆叶斯尔·哈斯木新疆维吾尔自治区妇幼保健院呼吸内分泌科新疆乌鲁木齐830000 新疆维吾尔自治区妇幼保健院冠心病肾病科新疆乌鲁木齐830000

目的探究2型糖尿病(type 2 diabetes,T2DM)患者肠道菌群特征及血清代谢指标与健康人群之间的差异,分析血清代谢组学特征与肠道菌群特征之间的相关性。方法纳入2021年6月至2022年12月新疆维吾尔自治区妇幼保健院就诊的100例初发T2DM患者... 详细信息

目的探究2型糖尿病(type 2 diabetes,T2DM)患者肠道菌群特征及血清代谢指标与健康人群之间的差异,分析血清代谢组学特征与肠道菌群特征之间的相关性。方法纳入2021年6月至2022年12月新疆维吾尔自治区妇幼保健院就诊的100例初发T2DM患者作为DM组;同期招募100例健康志愿者作为对照组。收集两组对象粪便和血清标本,采用高通量测序技术对粪便标本进行16S rRNA测序,分析T2DM患者肠道菌群整体结构及多样性特征,比较两组间的显著差异物种,运用PICRUSt工具对肠道差异菌群进行基因功能通路预测。采用GC-MS技术对两组对象血清标本进行代谢谱测定,分析两组对象血清代谢物之间的差异;利用Mectaboanalyst软件分析血清差异代谢物涉及的代谢通路;将T2DM患者血清中的差异代谢物与高通量测序得到的肠道菌群代谢通路数据进行相关性分析。结果肠道菌群α多样性方面,DM组Chao指数、Ace指数、Shannon指数低于对照组,Simpson指数高于对照组,差异有统计学意义(P<0.05);两组肠道菌群β多样性差异有统计学意义(P<0.05)。DM组患者肠道菌群中以肠球菌属(Enterococcus)、梭菌属(Clostridioides)、粪杆菌属(Faecalibacterium)、放线菌属(Actinomyces)、埃希菌属(Escherichia)、伊格尔兹菌属(Eggerthella)、安德克菌属(Adlercreutzia)占主要优势;对照组肠道菌群中以拟杆菌门(Bacteroidetes)、乳杆菌属(Lactobacillus)、双歧杆菌属(Bifidobacterium)、副杆菌属(Parabacteroides)、普雷沃菌(Prevotella)、甲烷短杆菌属(Methanobrevibacter)、二十碳孢梭菌属(Erysipelatoclostridium)占主要优势。代谢组学方面,DM组乳酸、亚油酸、棕榈酸、油酸水平高于对照组,L-丙氨酸、L-缬氨酸、络氨酸水平低于对照组,差异有统计学意义(P<0.05);PICRUSt工具对肠道差异菌群进行基因功能通路预测显示,肠道差异菌属可获得3个代谢通路信息,分别是其他次级代谢产物的合成、脂质代谢、碳水化合物代谢;分析血清差异代谢物涉及的代谢通路显示,共有9条差异通路,以涉及脂质代谢通路为主;比较两种代谢通路显示,脂质代谢通路为肠道菌群和血清代谢物的共有代谢通路。结论T2DM患者存在肠道菌群失调,肠球菌属、梭菌属、粪杆菌属含量增多,拟杆菌属、乳酸杆菌和双歧杆菌属含量减少;且T2DM患者和健康人群的代谢指标存在差异,肠道菌群可能通过影响脂质代谢来影响T2DM的进程。

关键词： 2型糖尿病肠道菌群代谢组学代谢通路

An Experimental Study on the Pressure Distribution in Horizontal Gas Wells

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Fluid Dynamics & Materials Processing 2020年第6期16卷 176-191页

作者： Jinbo Liu Ziheng Jiang Xuezhang Feng Ruiquan Liao Dianfang Feng Xingkai Zhang hasimu Aikeremu No.1 Gas Production Plant of Xinjiang Oilfield Company Karamay834000China School of Petroleum Engineering Yangtze UniversityWuhan430100China

Laboratory experiments have been carried out to study the fluid flow in the wellbore of a horizontal gas well during the production process.The related pressure distribution has been determined considering different cases(different inflow media,different perforation opening methods and different liquid holdup).It has been found that the larger the fluid flow rate,the greater the pressure changes in the wellbore under the same hole opening mode.The uniformity of the perforation opening method was also an important factor affecting the magnitude of the wellbore pressure change.The liquid holdup also affected the pressure distribution,especially when the gas volumetric flow rate exceeded 200 m3/h.Comparison of the outcomes of the present experimental study with the predictions of a theoretical model available in the literature has provided a relative error smaller than 20%.

关键词： Horizontal gas well laboratory experiment pressure distribution