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Journal of Clinical and Translational Hepatology 2022年第6期10卷 1138-1147页

作者： hao-zhen Ren Sen-Zhe Xia Xue-Qian Qin An-Yin Hu Jing-Lin Wang Department of Hepatobiliary Surgery Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjingJi-angsuChina Department of Hepatobiliary Surgery Nanjing Drum Tower Hospital Clinical College of Jiangsu UniversityNanjingJiangsuChina Department of Hepatobiliary Surgery Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western MedicineNanjing University of Chinese MedicineNanjingJiangsuChina

Background and Aims:Hepatic ischemic reperfusion in-jury(IRI)occurring during surgery seriously affects patient *** specific mechanism of IRI has not been fully *** study aim was to explore the changes of in-flammatory environment,and the relationship of the Th17/Treg cell ratio and FOXO1 expression in hepatic ***:Liver samples at different ischemic times were collected from patients and *** expression of inflammatory markers and FOXO1 in the liver was detected by western blotting and *** changes of liver lymphocytes were analyzed by flow *** AKT/Stat3/FOXO1 pathway was veri-fied by targeting AKT with *** role of FOXO1 in liver inflammation and changes in lymphocyte phenotype was confirmed by upregulating FOXO1 with ***-sults:Prolonged ischemic time aggravates liver injury in both humans and mouse models of hepatic ***-stress caused Th17/Treg imbalance and FOXO1 down-regulation by activat-ing the AKT/Stat3/FOXO1 signaling *** of FOXO1 reversed the Th17/Treg cytokine imbalance and altered the inflammation environment in the ***:Liver IRI induced Th17/Treg *** of FOXO1 re-versed the imbalance and alleviated liver inflammation.

关键词： Liver ischemia-reperfusion injury Inflammatory factors Th17 Treg AKT/Stat3/FOXO1 pathway

Differences in biochemical responses to cold stress in two contrasting varieties of rape seed (Brassica napus L.)

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Forestry Studies in China 2007年第2期9卷 142-146页

作者： Zou Wang-hao Chen Yu-zhen Lu Cun-fu College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 P. R. China

Changes in isozymes of peroxidase, catalase, superoxide dismutase, polyphenol oxidase, and esterase and activities of per- oxidase and polyphenol oxidase in two annual Brassica varieties （AS-3 and CON, cold-tolerant and cold-sensitive, respectively） after 4℃ treatment were assayed. After the low temperature treatment, PAGE patterns of isozymes of peroxidase, catalase, superoxide dismutase, polyphenol oxidase, and esterase showed that the expressions and activities of these enzymes in the AS-3 variety were higher than the CON variety. Under normal and cold conditions, peroxidase and esterase activities of AS-3 were higher than those of CON. The relative freezing tolerance of these two cultivars was discussed.

关键词： spring rape cold stress isozyme

Targeted migration of mesenchymal stem cells modified with CXCR4 to acute failing liver improves liver regeneration

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World Journal of Gastroenterology 2014年第40期20卷 14884-14894页

作者： Hu-Cheng Ma Xiao-Lei Shi hao-zhen Ren Xian-Wen Yuan Yi-Tao Ding Department of Hepatobiliary Surgery the Affiliated Drum Tower Hospital of Nanjing University Medical School Key Medical Center for Hepatobiliary Diseases of Jiangsu Province

AIM: To improve the colonization rate of transplanted mesenchymal stem cells (MSCs) in the liver and effect of MSC transplantation for acute liver failure (ALF).

关键词： Acute liver failure Cell transplantation Chemokine CXC receptor 4 Mesenchymal stem cells Cell mobilization

Intraportal mesenchymal stem cell transplantation prevents acute liver failure through promoting cell proliferation and inhibiting apoptosis

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Hepatobiliary & Pancreatic Diseases International 2016年第6期15卷 602-611页

作者： Jian-Feng Sang Xiao-Lei Shi Bin Han Tao Huang Xu Huang hao-zhen Ren Yi-Tao Ding Department of General Surgery the Affiliated Drum Tower Hospital of Nanjing University Medical School

BACKGROUND： Transplantation of mesenchymal stem cells （MSCs） has been regarded as a potential treatment for acute liver failure （ALF）, but the optimal route was unknown. The present study aimed to explore the most effective MSCs transplantation route in a swine ALF model. METHODS： The swine ALF model induced by intravenous injection of D-Gal was treated by the transplantation of swine MSCs through four routes including intraportal injection （InP group）, hepatic intra-arterial injection （AH group）, peripheral intravenous injection （PV group） and intrahepatic injection （IH group）. The living conditions and survival time were recorded. Blood samples before and after MSCs trans- plantation were collected for the analysis of hepatic function. The histology of liver injury was interpreted and scored in terminal samples. Hepatic apoptosis was detected by TUNEL assay. Apoptosis and proliferation related protein expressions including cleaved caspase-3, survivin, AKT, phospho-AKT （Ser473）, ERK and phospho-ERK （Tyr204） were analyzed by Western blotting. RESULTS： The average survival time of each group was 10.7± 1.6 days （InP）, 6.0±0.9 days （AH）, 4.7±1.4 days （PV）, 4.3± 0.8 days （IH）, respectively, when compared with the average survival time of 3.8±0.8 days in the D-Gal group. The survival rates between the InP group and D-Gal group revealed a statistically significant difference （P〈0.01）. Pathological and biochemical analysis showed that liver damage was the worst in the D-Gal group, while less injury in the InP group. Histopathological scores revealed a significant decrease in the InP group （3.17±1.04, P〈0.01） and AH group （8.17±0.76, P〈0.05） as compared with that in the D-Gal group （11.50±1.32）. The apoptosis rate in the InP group （25.0%±3.4%, P〈0.01） and AH group （40.5%±1.0% , P〈0.05） was lower than that in the D-Gal group （70.6%±8.5%）. The expression of active caspase-3 was inhibited, while the expression of survivin, AKT, phospho- AKT （Ser473）, ERK and phospho-ERK （Tyr20

关键词： mesenchymal stem cells stem cell transplantation acute liver failure apoptosis regeneration

Psychometrics of chronic liver disease questionnaire in Chinese chronic hepatitis B patients

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World Journal of Gastroenterology 2013年第22期19卷 3494-3501页

作者： Kai-Na Zhou Min Zhang Qian Wu zhen-hao Ji Xiao-Mei Zhang Gui-Hua Zhuang Department of Epidemiology and Biostatistics Xi'an Jiaotong University College of Medicine

AIM: To evaluate psychometrics of the Chinese (mainland) chronic liver disease questionnaire (CLDQ) in patients with chronic hepatitis B (CHB). METHODS: A cross-sectional sample of 460 Chinese patients with CHB was selected from the Outpatient Department of the Eighth Hospital of Xi'an, including CHB (CHB without cirrhosis) (n = 323) and CHB-related cirrhosis (n = 137). The psychometrics includes reliability, validity and sensitivity. Internal consistency reliability was measured using Cronbach's α. Convergent and discriminant validity was evaluated by item-scale correlation. Factorial validity was explored by principal component analysis with varimax rotation. Sensitivity was assessed using Cohen's effect size (ES), and independent sample t test between CHB and CHB-related cirrhosis groups and between alanine aminotransferase (ALT) normal and abnormal groups after stratifying the disease (CHB and CHB-related cirrhosis).RESULTS: Internal consistency reliability of the CLDQ was 0.83 (range: 0.65-0.90). Most of the hypothesized item-scale correlations were 0.40 or over, and all of such hypothesized correlations were higher than the alternative ones, indicating satisfactory convergent and discriminant validity. Six factors were extracted after varimax rotation from the 29 items of CLDQ. The eligible Cohen's ES with statistically significant independent sample t test was found in the overall CLDQ and abdominal, systematic, activity scales (CHB vs CHBrelated cirrhosis), and in the overall CLDQ and abdominal scale in the stratification of patients with CHB (ALT normal vs abnormal). CONCLUSION: The CLDQ has acceptable reliability, validity and sensitivity in Chinese (mainland) patients with CHB.

关键词： Chronic hepatitis B Chronic liver disease questionnaire Reliability Validity Sensitivity

Antitumor effects of different Ganoderma lucidum spore powder in cell-and zebrafish-based bioassays

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Journal of Integrative Medicine 2021年第2期19卷 177-184页

作者： Yue-jiao Shi Hua-xian zheng Zhu-ping Hong Han-bo Wang Ying Wang Ming-yan Li zhen-hao Li Zhejiang Shouxiangu Pharmaceutical Co. LtdWuyi 321200Zhejiang ProvinceChina Zhejiang Shouxiangu Botanical Drug Institute Co. LtdHangzhou 310012Zhejiang ProvinceChina Zhejiang Engineering Research Center of Rare Medicinal Plants Wuyi 321200Zhejiang ProvinceChina Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou 310012Zhejiang ProvinceChina

Objective:Ganoderma lucidum spore(GLS)is gaining recognition as a medicinal part of *** and has been reported to possess various pharmacological properties,such as antitumor *** this work,wall-broken GLS powder(BGLSP)and wall-removed GLS powder(RGLSP),two kinds of GLS powder with different manufacturing techniques,were compared in terms of contents of active constituents and in vivo and in vitro antitumor ***:The ultraviolet and visible spectrophotometry method was used to determine the contents of polysaccharides and total triterpenoids in BGLSP and *** individual triterpenoids were further quantified using ultra-high-performance liquid chromatography and quantitative analysis of multicomponents by single *** antitumor effects of BGLSP and RGLSP were evaluated using in vitro cell viability assay against human gastric carcinoma SGC-7901,lung carcinoma A549 and lymphoma Ramos and further validated by in vivo zebrafish xenograft models with transplanted SGC-7901,A549 and Ramos,Results:The results showed that the contents of polysaccharides,total triterpenoids and individual triterpenoids of RGLSP were significantly higher than those of *** both BGLSP and RGLSP inhibited the three tumor cell lines in vitro in a dose-dependent manner,the inhibitory effects of RGLSP were much better than those of *** the in vivo zebra fish assay,RGLSP exhibited more potent inhibitory activities against tumors transpla nted into the zebra fish compared with BGLSP,and the inhibition rates of RGLSP reached approximately 78%,31%and 83%on SGC-7901,A549 and Ramos,***:The results indicated that the antitumor effects of GLS were positively correlated with the contents of the polysaccha rides and triterpenoids and demonstrated that the wall-removing manufacturing technique could significantly improve the levels of active constituents,and thereby enhance the antitumor activity.

关键词： Ganoderma lucidum Tumor Cancer cell Zebrafish Wall-broken Ganoderma lucidum spore powder Wall-removed Ganoderma lucidum spore powder

Analysis of Brucella cellular fatty acids

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Frontiers of Medicine 2010年第2期4卷 216-219页

作者： zhen-Xiang Zhao Hai JIANG Bu-Yun CUI Hong-Yan Zhao Dong-Ri PIAO Lan-Yu LI Su-zhen hao Department of Microbiology and Immunology Shanxi Medical UniversityTaiyuan 030001China National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China

To acquire data of Brucella cellular fatty acids(CFAs)and probe into the possibility of utilizing CFAs information in typing Brucella,19 reference strains were subjected to CFAs *** all strains were inoculated on Brucella Agar plates,the cells were harvested,saponiﬁcated,methylated and extracted to provide fatty acid methylesters for gas chromatography(GC)*** on the CFAs data matrix,a dendrogram of 19 reference strains was generated by SPSS11.5 software *** results showed that 19 reference strains were divided intoﬁve clusters:cluster 1 included ***(bv.1,2,3,5)and ***;cluster 2 included ***(bv.3,4,5,6)and ***(bv.1,2,3);cluster 3 included ***(bv.1,2,7,9)and ***;cluster 4 was ***(bv.4);and cluster 5 was *** Brucella by GC analysis of CFAs is a good method to reﬂect drug resistance of Brucella,and the classiﬁcation is beneﬁcial for clinical *** also provides a new result of typing and demonstrates that the traditional classiﬁcation is not completely *** analysis may identify ***(bv.4)and ***.

关键词： Brucella gas chromatography cellular fatty acids dendrogram typing

Revegetation of Copper Mine Tailings with Ryegrass and Willow

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Pedosphere 2004年第3期14卷 283-288页

作者： haoXiu-zhen ZHOUDong-Mei SIYou-Bin StateKeyLaboratoryofSoilandSustainableAgriculture InstituteofSoilScienceChineseAcademyofSciencesNanjing210008（China）

To restore vegetation on metal mine tailings is very difficult because theyoften contain high concentrations of heavy metals, low nutrient content and low water retentioncapacity. This study involved 3 experiments that evaluated the effects of 4 treatment amendments:montmorillonite, rice straw, organic manure and chemical fertilizer on the growth of ryegrass(Lolium perenne L.) and willow (Salix viminalis L.) with Cu and Zn mine tailings from two miningareas. The results showed that ryegrass was the most tolerant of 4 crops to Cu toxicity. Also whenorganic manure, which contained high concentrations of inorganic salts, was added to the minetailings, it significantly hindered ryegrass growth (P = 0.05). Meanwhile, with ryegrass organicmanure significantly increased (P = 0.05) the extractable Cu concentration in both mine *** montmorillonite was used as a mine tailings amendment with willow, the height and tress numberat the 1st cut were significantly greater (P = 0.05) than a control without montmorillonite. Howeverthere was no significant difference for height, tress number, dry weight or root dry weight at the2nd cut. So, amendment applications to reduce metal toxicity and increase nutrients retention inmine tailings were essential during revegetation of mine tailings.

关键词： amendment copper mine tailings ryegrass (lolium perenne L.) willow (salixviminalis L.) zinc mine tailings

Phase-modulated quadrature squeezing in two coupled cavities containing a two-level system

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Chinese Physics B 2020年第5期29卷 125-131页

作者： hao-zhen Li Ran Zeng Xue-Fang Zhou Mei-Hua Bi Jing-Ping Xu Ya-Ping Yang School of Communication Engineering Hangzhou Dianzi UniversityHangzhou 310018China Key Laboratory of Advanced Micro-Structured Materials of Ministry of Education School of Physics Science and EngineeringTongji UniversityShanghai 200092China

The phase-modulated quadrature squeezing in the system that composed of two coupled cavities interacting with a two-level atom is *** variances of the amplitude and phase quadrature of the output field are *** turns out that the squeezing behaviors of the output field can be obviously modified due to the phase difference of the coupling strengths between the atom and the two *** squeezing in one quadrature(i.e.,phase quadrature)can be transferred into another(i.e.,amplitude quadrature),or the quadrature squeezing located at the low-frequency region can be transferred into the high-frequency region by modulating the relative phase of the coupling ***,the effects of the decay mismatch between the two cavities and the coupling mismatch between the atom and the cavities on the quadrature squeezing have been *** results show that both the decay mismatch and the coupling mismatch play a positive role in generating better quadrature squeezing.

关键词： quadrature squeezing cavity QED phase modulation

Combined mesenchymal stem cell transplantation and interleukin-1 receptor antagonism after partial hepatectomy

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World Journal of Gastroenterology 2016年第16期22卷 4120-4135页

作者： Jian-Feng Sang Xiao-Lei Shi Bing Han Xu Huang Tao Huang hao-zhen Ren Yi-Tao Ding Department of General Surgery the Affiliated Drum Tower Hospital of Nanjing University Medical School

AIM: To study the therapeutic effects of mesenchymal stem cells (MSCs) and an interleukin-1 receptor antagonist (IL-1Ra) in acute liver ***: Chinese experimental miniature swine (15 ± 3 kg, 5-8 mo) were obtained from the Laboratory Animal Centre of the Affiliated Drum Tower Hospital of Nanjing University Medical School. Acute liver failure was induced via 85% hepatectomy, and animals were treated by MSC transplantation combined with IL-1Ra injection. Blood samples were collected for hepatic function analysis, and the living conditions and survival time were recorded. Liver injury was histologically analyzed. Hepatic cell regeneration and apoptosis were studied by Ki67 immunohistochemistry and terminal deoxynucleotidyl transferase dUTP nick end labeling, respectively. The levels of protein kinase B and nuclear factor-κB expression were analyzed by Western ***: MSCs were infected with a lentivirus for expression of green fluorescent protein (GFP) for subsequent identification; 97.3% of the MSCs were positive for GFP as assessed by flow cytometry. Additional flow cytometric analysis of cell surface marker expression demonstrated that > 90% of GFP-expressing MSCs were also positive for CD29, CD44, and CD90, indicating that most of these cells expressed typical markers of MSCs, and the population of MSCs was almost pure. Transplantation of MSCs in combination with 2 mg/kg IL-1Ra therapy significantly improved survival time compared to the acute liver failure model group (35.3 ± 6.7 d vs 17.3 ± 5.5 d, P < 0.05). Combined therapy also promoted improvement in serum inflammatory cytokines and biochemical conditions. The observed hepatic histopathologic score was significantly lower in the group with combined therapy than in the model group (3.50 ± 0.87 vs 8.17 ± 1.26, P < 0.01). In addition, liver cell apoptosis in the combined therapy group was significantly inhibited (18.1 �

关键词： Mesenchymal stem cells Interleukin-1 receptor antagonist Stem cell transplantation Acute liver failure Hepatoectomy