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Cell specialization and coordination in Arabidopsis leaves upon pathogenic attack revealed by scRNA-seq

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Plant Communications 2023年第5期4卷 63-78页

作者： Etienne Delannoy Bastien Batardiere Ste phanie Pateyron Ludivine Soubigou-Taconnat julien Chiquet Jean Colcombet julien Lang Universite Paris-Saclay CNRSINRAEUniversite EvryInstitute of Plant Sciences Paris-Saclay(IPS2)91190 Gif sur YvetteFrance Universite Paris Cite CNRSINRAEInstitute of Plant Sciences Paris-Saclay(IPS2)91190 Gif sur YvetteFrance UMR MIA Paris-Saclay Universite Paris-SaclayAgroParisTechINRAE91120 PalaiseauFrance

Plant defense responses involve several biological processes that allow plants to fight against pathogenic *** these different processes are orchestrated within organs and depend on specific cell types is poorly ***,using single-cell RNA sequencing(scRNA-seq)technology on three independent biological replicates,we identified several cell populations representing the core transcriptional responses of wild-type Arabidopsis leaves inoculated with the bacterial pathogen Pseudomonas syringae *** these populations,we retrieved major cell types of the leaves(mesophyll,guard,epidermal,companion,and vascular S cells)with which we could associate characteristic transcriptional reprogramming and regulators,thereby specifying different cell-type responses to the *** analyses of transcriptional dynamics,on the basis of inference of cell trajectories,indicated that the different cell types,in addition to their characteristic defense responses,can also share similar modules of gene reprogramming,uncovering a ubiquitous antagonism between immune and susceptible ***,it appears that the defense responses of vascular S cells,epidermal cells,and mesophyll cells can evolve along two separate paths,one converging toward an identical cell fate,characterized mostly by lignification and detoxification *** this divergence does not correspond to the differentiation between immune and susceptible cells,we speculate that this might reflect the discrimination between cellautonomous and non-cell-autonomous *** our data provide an upgraded framework to describe,explore,and explain the specialization and the coordination of plant cell responses upon pathogenic challenge.

关键词： scRNA-seq plant defense responses plant immunity plant susceptibility Arabidopsis/Pseudomonas interactions biotic stress

Production of micro-cuttings from acorns to test the plasticity of response to contrasting soil water regimes

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Journal of Forestry Research 2016年第5期27卷 995-1001页

作者： julien parelle sara belabbes fabienne tatin-froux UMR Chrono-Environnement 6249 Universite de Bourgogne Franche-Comte/CNRS USC INRA 16 route de Gray 25030 Besancon Cedex France

We collected acorns from selected Quercus robur and Quercus petraea mother trees growing at sta- tions with different depths to the water table. We produced pairs of micro-cuttings from these single acorns. The obtained pairs of cuttings represented phenotypes of high similarity according to photosynthetic parameters. These pairs of cuttings were used to test the plasticity of response to contrasting water regimes. For each pair, one cutting was submitted to drought and the other was submitted to waterlogging. Drought response was recorded according to gas exchange traits （stomatal closure and water use effi- ciency）. The response to waterlogging was recorded according to the capacity to form hypertrophied lenticels and to maintain the CO2 assimilation rate. Intermediary phenotypes presenting both high water use efficiency and the capacity to develop hypertrophied lenticels were expected.

关键词： Acorns Drought petraea Quercus sessiliflora Quercus robur Quercus Waterlogging

Deterministic and probabilistic analysis of great-depth braced excavations:A 32 m excavation case study in Paris

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第5期16卷 1505-1521页

作者： Tingting Zhang julien Baroth Daniel Dias Khadija Nejjar Université Grenoble Alpes CNRSGrenoble INP3SRGrenoble38000France Antea Group Antony92160France School of Automotive and Transportation Engineering Hefei University of TechnologyHefei23009China Terrasol 42-52 Quai de la RapéeParis75012France

The Fort d’Issy-Vanves-Clamart(FIVC)braced excavation in France is analyzed to provide insights into the geotechnical serviceability assessment of excavations at great depth within deterministic and probabilistic *** FIVC excavation is excavated at 32 m below the ground surface in Parisian sedimentary basin and a plane-strain finite element analysis is implemented to examine the wall deflections and ground surface settlements.A stochastic finite element method based on the polynomial chaos Kriging metamodel(MSFEM)is then proposed for the probabilistic *** with field measurements and former studies are carried *** academic cases are then conducted to investigate the great-depth excavation stability regarding the maximum horizontal wall deflection and maximum ground surface *** results indicate that the proposed MSFEM is effective for probabilistic analyses and can provide useful insights for the excavation design and construction.A sensitivity analysis for seven considered random parameters is then *** soil friction angle at the excavation bottom layer is the most significant one for *** soil-wall interaction effects on the excavation stability are also given.

关键词： Braced deep excavation Soil-wall interaction Stochastic finite element method Horizontal wall deflection Settlement Failure probability

A phase-locked loop using ESO-based loop filter for grid-connected converter: performance analysis

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Control Theory and Technology 2021年第1期19卷 49-63页

作者： Baoling Guo Seddik Bacha Mazen Alamir julien Pouget School of Engineering.(HES SO)Universily of Applied Sciences and Arts of Western Switzeland Sion1950 ValaisSwitzerland Institute of Engineering Grenoble University Grenoble AlpesCNRSG2Elab38000 GrenobleFrance Institute of Engineering Grenoble University Grenoble AlpesCNRSGIPSA-Lab38400 Saint Martin D'HeresFrance

An extended state observer(ESO)-based loop filter is designed for the phase-locked loop(PLL)involved in a disturbed grid-connected converter(GeC).This ESO-based design enhances the performances and robustness of the PLL,and,therefore,improves control performances of the disturbed ***,the ESO-based LF can be applied to PLLs with extra filters for abnormal grid *** unbalanced grid is particularly taken into account for the performance analysis.A tuning approach based on the well-designed PI controller is discussed,which results in a fair comparison with conventional PI-type *** frequency domain properies are quantitatively analysed with respeet to the control stability and the noises *** frequency domain analysis and simulation results suggesti that the performances of the generated ESO-based controllers are comparable to those of the PI control at low frequency,while have better ability to atenuate high-frequency measurement *** phase margin decreases slightly,but remains ***,experimental tests are conducted with a hybrid power hardwarein-the-loop benchmark,in which balanced/unbalanced cases are both *** obtained results prove the effectiveness of ESO based PLLs when applied to the disturbed GeC.

关键词： Grid-conected converters Grid disturbances Phase-locked loop Loop filter Extended state observer Tuning approach Stability analysis Phase margin Noises attenuation Power hardware-in-the-loop(PHIL)test

Intermediate filament dynamics： Disassembly regulation

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International Journal of Biomathematics 2017年第1期10卷 273-294页

作者： Chengjun Sun julien Arino Stephanie Portet School of Management and EconomicsKunming University of Science and Technology Kunming P. R. China Department of Mechanical Engineering University of Hong Kong Pokfulam Road Hong Kong Department of Mathematics University of Manitoba Winnipeg Manitoba R3T 2N2 Canada

A mechanism of intermediate filament disassembly regulation is proposed in which disas- sembly is regulated by the amount of proteins assembled in networks. It is Mso hypothe- sized that a delay might exist between regulation and actuM disassembly. Under realistic biological conditions of assembly and disassembly, it is shown that such a delay is harm- less and does not destabilize the organization of intermediate filaments in networks. However, for high rates of disassembly, the model predicts that delay can destabilize the organization, with the intermediate filament material oscillating between organizations mainly in networks and in nonfilamentous particles.

关键词： Cytoskeleton organization intermediate filaments time delay global asymp-totic stability bifurcation periodic solution.

SCHOOL DIFFICULTIES in Children Cancer Survivors: A Narrative Review and a Teacher Point-of-View

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Psycho-Oncologie 2024年第2期18卷 109-115页

作者： Fanny Delehaye Caroline Fayet julien Lejeune Department of Medicine University of CaenCaen14000France Department of Pediatric Oncology University Hospital of CaenCaen14000France Laboratory“ANTICIPE”UNIT U1086 INSERM Caen14000France Department of Pediatric Oncology Clocheville University HospitalTours37000France

School difficulties are common in children cancer survivors and may be prognostic for the success of the patient social reintegration after the ***,we carry out a narrative review of the literature from 2001 to 2022 to assess the school difficulties of these patients,the predictive factors of these difficulties,and the possible long-term *** punctuate this review the practical point-of-view of a teacher dedicated to our hospital,who works with children with chronic diseases such as ***,we expose possible barriers and solutions to improve the scholar reintegration and limit the impact of cancer on the academic achievement.

关键词： Cancer children school

Inter- and intraspecific variability of plant individual growth and its role on species ranking in grasslands

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Journal of Plant Ecology 2020年第3期13卷 378-386页

作者： Rémi Perronne Franck Jabot julien Pottier UCA INRAEVetAgro SupUMR Ecosystème Prairialsite de Crouel5 chemin de BeaulieuF-63000 Clermont-FerrandFrance UCA INRAEUR LISCCentre de Clermont-Ferrand9 avenue Blaise PascalF-63178 AubièreFrance

Aims Individual growth constitutes a major component of individual ***,measuring growth rates of herbaceous plants non-destructively at the individual level is notoriously *** study,based on an accurate non-destructive method of aboveground biomass estimation,aims to assess individual relative growth rates(RGRs)of some species,identify its environmental drivers and test its consequences on community *** specifically address three questions:(i)to what extent environmental conditions explain differences in individual plant growth between sites,(ii)what is the magnitude of intraspecific variability of plant individual growth within and between sites and(iii)do species-averaged(dis-)advantage of individual growth compared with the whole vegetation within a site correlate with species ranking at the community level?Methods We monitored the growth of individuals of four common perennial species in 18 permanent grasslands chosen along a large pedoclimatic gradient located in the Massif Central,*** measured soil properties,levels of resources and meteorological parameters to characterize environmental conditions at the site *** design enables us to assess the influence of environmental conditions on individual growth and the relative extent of inter-individual variability of growth explained within and between *** determined the ranking of each of the four species in each site with botanical surveys to assess the relationship between species-averaged growth(dis-)advantage relative to the whole community and species rank in the *** Findings We found that environmental conditions explain a significant proportion of individual growth variability,and that this proportion is strongly variable between *** availability was the main driver of plant growth,followed by rainfall amount and potential evapotranspiration,while soil properties had only a slight *** further highlighted a moderate to high wit

关键词： relative growth rate species ranking non-destructive biomass estimation temperate permanent grasslands inter-individual variability

Phase Invariant Proper Orthogonal Decomposition for the Study of a Compressed Vortex

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Journal of Thermal Science 2005年第2期14卷 108-113页

作者： julien MOREAU Mark FOGLEMAN G.CHARNAY J.BOREE Institut de Mécanique des Fluides UMR CNRS/INPT-UPS 5502 Allée du Pr Soula 31400 Toulouse FranceCornell University IthacaNew York 14853U.S.A.Institut de Mécanique des Fluides UMR CNRS/INPT-UPS 5502 Allée du Pr Soula 31400 ToulouseFranceLEA ENSMA UMR 6609 CNRS Futuroscope France

We propose here an experimental investigation of a vortex submitted to a radial perturbation while being compressed. This experiment reproduces a model situation of the complex flows that take place in a real engine cylinder. An isolated tumbling flow is first submitted to an injection of fluid and then compressed and measurements are realised by Particle Image Velocimetry (PIV). The Proper Orthogonal Decomposition is known to be an unbiased method to identify the coherent structures of turbulent flows. It is possible to make this decomposition for a given phase or to create a set of basis functions with the whole set of compression stroke velocity ***, the experimental set-up will be presented, then the second part provides the POD principle. Results of phased POD decomposition of the compression of the unperturbated vortex will be presented. Finally results from the time invariant POD will be discussed to study the influence of the jet perturbation in the compression of a vortex.

关键词： turbulence POD PIV cycle to cycle fluctuations.

Machine learning in vehicular networking:An overview

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Digital Communications and Networks 2022年第1期8卷 18-24页

作者： Kang Tan Duncan Bremner julien Le Kernec Lei Zhang Muhammad Imran James Watt School of Engineering University of Glasgow GlasgowG128QQj UK

As vehicle complexity and road congestion increase,combined with the emergence of electric vehicles,the need for intelligent transportation systems to improve on-road safety and transportation efficiency using vehicular networks has become *** evolution of high mobility wireless networks will provide improved support for connected vehicles through highly dynamic heterogeneous ***,5G deployment introduces new features and technologies that enable operators to capitalize on emerging infrastructure *** Learning(ML),a powerful methodology for adaptive and predictive system development,has emerged in both vehicular and conventional wireless *** data-centric methods enables ML to address highly dynamic vehicular network issues faced by conventional solutions,such as traditional control loop design and optimization *** article provides a short survey of ML applications in vehicular networks from the networking *** topics covered in this article include network control containing handover management and routing decision making,resource management,and energy efficiency in vehicular *** findings of this paper suggest more attention should be paid to network forming/deforming decision *** applications in vehicular networks should focus on researching multi-agent cooperated oriented methods and overall complexity reduction while utilizing enabling technologies,such as mobile edge computing for real-world *** datasets,simulation environment standardization,and method interpretability also require more research attention.

关键词： Vehicular networks Machine learning Vehicle-to-everything(V2X) Networking Handover management Resource allocation Energy efficiency