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FK506 contributes to peripheral nerve regeneration by inhibiting neuroinflammatory responses and promoting neuron survival

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Neural Regeneration Research 2025年第7期20卷 2108-2115页

作者： Yuhui Kou Zongxue Jin Yusong Yuan bo Ma Wenyong Xie Na han Department of Trauma and Orthopedics Peking University People’s HospitalBeijingChina National Center for Trauma Medicine Peking University People’s HospitalBeijingChina Key Laboratory of Trauma and Neural Regeneration(Peking University) Ministry of EducationBeijingChina Trauma Medicine Center Peking University People’s HospitalBeijingChina Department of Orthopedics China-Japan Friendship HospitalBeijingChina

FK506(Tacrolimus)is a systemic immunosuppressant approved by the *** and Drug ***506 has been shown to promote peripheral nerve regeneration,however,its precise mechanism of action and its pathways remain *** this study,we established a rat model of sciatic nerve injury and found that FK506 improved the morphology of the injured sciatic nerve,increased the numbers of motor and sensory neurons,reduced inflammatory responses,markedly improved the conduction function of the injured nerve,and promoted motor function *** findings suggest that FK506 promotes peripheral nerve structure recovery and functional regeneration by reducing the intensity of inflammation after neuronal injury and increasing the number of surviving neurons.

关键词： FK506 inflammation motor neurons nerve regeneration neuron peripheral nerve injury sensory neurons

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多相导电混凝土的受弯开裂自监测性能

华南理工大学学报（自然科学版） 2017年第8期45卷 132-138页

作者：丁一宁衡震韩知伯大连理工大学海岸与近海工程国家重点实验室辽宁大连116024 中广电广播电影电视设计研究院北京100045

将结构型钢纤维(SF)、碳纤维(CF)及纳米炭黑(NCB)作为多相导电材料加入混凝土中,研究了多相导电材料对混凝土开裂后的力学性能、导电性能及裂缝扩展自监测性能的影响.建立了混凝土梁的受拉区表面电阻变化率(FCR)与裂缝张开位移(COD)之... 详细信息

将结构型钢纤维(SF)、碳纤维(CF)及纳米炭黑(NCB)作为多相导电材料加入混凝土中,研究了多相导电材料对混凝土开裂后的力学性能、导电性能及裂缝扩展自监测性能的影响.建立了混凝土梁的受拉区表面电阻变化率(FCR)与裂缝张开位移(COD)之间的关系.结果表明:导电相材料的加入能够显著提高抗弯强度与弯曲韧性;多相导电材料对弯曲性能及裂缝扩展的自监测性能具有正混杂效应;电阻变化率与裂缝张开位移呈线性关系.

关键词：纤维纳米炭黑混凝土开裂自监测

AAV2-PDE6B restores retinal structure and function in the retinal degeneration 10 mouse model of retinitis pigmentosa by promoting phototransduction and inhibiting apoptosis

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Neural Regeneration Research 2025年第8期20卷 2408-2419页

作者： Ruiqi Qiu Mingzhu Yang Xiuxiu Jin Jingyang Liu Weiping Wang Xiaoli Zhang Jinfeng han bo Lei Henan Eye Hospital Henan Provincial People’s HospitalPeople’s Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina Eye Institute Henan Academy of Innovations in Medical ScienceZhengzhouHenan ProvinceChina Branch of National Clinical Research Center for Ocular Disease Henan Provincial People’s HospitalZhengzhouHenan ProvinceChina Academy of Medical Science Zhengzhou UniversityZhengzhouHenan ProvinceChina

Retinitis pigmentosa is a group of inherited diseases that lead to retinal degeneration and photoreceptor cell ***,there is no effective treatment for retinitis pigmentosa caused by PDE6B ***-associated virus(AAV)-mediated gene therapy is a promising strategy for treating retinitis *** aim of this study was to explore the molecular mechanisms by which AAV2-PDE6B rescues retinal *** do this,we injected retinal degeneration 10(rd10)mice subretinally with AAV2-PDE6B and assessed the therapeutic effects on retinal function and structure using dark-and light-adapted electroretinogram,optical coherence tomography,and ***-independent acquisition-mass spectrometry-based proteomic analysis was conducted to investigate protein expression levels and pathway enrichment,and the results from this analysis were verified by real-time polymerase chain reaction and western ***2-PDE6B injection significantly upregulated PDE6βexpression,preserved electroretinogram responses,and preserved outer nuclear layer thickness in rd10 *** expressed proteins between wild-type and rd10 mice were closely related to visual perception,and treating rd10 mice with AAV2-PDE6B restored differentially expressed protein expression to levels similar to those seen in wild-type *** Encyclopedia of Genes and Genome analysis showed that the differentially expressed proteins whose expression was most significantly altered by AAV2-PDE6B injection were enriched in phototransduction ***,the phototransductionrelated proteins Pde6α,Rom1,Rho,Aldh1a1,and Rbp1 exhibited opposite expression patterns in rd10 mice with or without AAV2-PDE6B ***,Bax/Bcl-2,p-ERK/ERK,and p-c-Fos/c-Fos expression levels decreased in rd10 mice following AAV2-PDE6B *** data suggest that AAV2-PDE6B-mediated gene therapy promotes phototransduction and inhibits apoptosis by inhibiting the ERK signaling pathway and upr

关键词： apoptosis AAV2-PDE6B ERK1/2 gene therapy phototransduction proteomics rd10 retinitis pigmentosa

Adsorption Characteristics of H2 on Molecular Sieves 5A at 77 K

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Adsorption Characteristics of H2 on Molecular Sieves 5A at 7...

不同供者来源及不同传代次数人脂肪间充质干细胞端粒酶的活性检测

作者： XIA Ti-rui YANG Hong-guang LIU Wen-jun LIU Zhen-xing ZHAO Wei-wei han zhi-bo Department of Reactor Engineering Research and Design

With the aim of applying cryogenic molecular sieves bed(CMSB)to a tritium extraction system(TES)for ITER TBM,adsorption characteristics of H on a molecular sieve at 77 K was investigated by a volumetric *** adsorption isotherms for H in a pressure range between about 1 and 10Pa was shown in Fig.1,and alkaline treatment(CaA includes not only Nabut also Ca)improved the adsorption

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中国组织工程研究与临床康复 2009年第45期13卷 8825-8828页

作者：池颖王有为韩之波韩忠朝中国医学科学院北京协和医学院血液学研究所实验血液学国家重点实验室天津市300020 细胞产品国家工程研究中心天津昂赛细胞基因工程有限公司天津市300457 中国医学科学院血液学研究所泰达生命科学技术研究中心天津市300457

背景:目前对于脂肪间充质干细胞端粒酶的表达尚无统一定论。目的:探讨不同供者来源和不同传代次数的成人脂肪间充质干细胞是否表达端粒酶活性。设计、时间及地点:细胞学体外观察,于2009-08在中国医学科学院血液学研究所泰达生命科学技... 详细信息

背景:目前对于脂肪间充质干细胞端粒酶的表达尚无统一定论。目的:探讨不同供者来源和不同传代次数的成人脂肪间充质干细胞是否表达端粒酶活性。设计、时间及地点:细胞学体外观察,于2009-08在中国医学科学院血液学研究所泰达生命科学技术研究中心完成。材料:成人脂肪组织来源于天津怡丽医学美容整形门诊接受抽脂术的5例健康供者,Hela细胞由中国医学科学院基础医学研究所基础医学细胞中心提供。方法:分别从每例健康供者抽取20mL脂肪组织,胶原酶消化法体外分离培养人脂肪间充质干细胞,待细胞生长至80%融合时胰酶消化传代。以Hela细胞作为对照,加入含体积分数为10%胎牛血清的1640培养基,待细胞生长至80%融合时胰酶消化传代。主要观察指标:人脂肪间充质干细胞的形态、表型、分化潜能,RT-PCR法检测人脂肪间充质干细胞端粒酶基因的表达,TRAP-ELISA法检测人脂肪间充质干细胞的端粒酶活性。结果:实验分离得到的人脂肪间充质干细胞呈贴壁生长,显微镜下呈梭形,当细胞密度较大时表现出漩涡状生长;CD19,CD34,CD11b,CD45,HLA-DR呈阴性表达,CD73,CD90,CD105呈阳性表达;成脂诱导后油红O染色可见明显红色油滴,成骨诱导后VonKossa染色可见黑色颗粒,提示有钙沉积。P1,P4,P7代人脂肪间充质干细胞均不表达端粒酶反转录酶基因,5例不同供者来源的脂肪间充质干细胞亦均不表达端粒酶反转录酶基因。Hela细胞具有端粒酶活性,人脂肪间充质干细胞不表达端粒酶活性。结论:不同供者来源和不同传代次数的人脂肪间充质干细胞均不表达端粒酶mRNA,也无端粒酶活性。

关键词：间充质干细胞脂肪端粒酶

Deciphering fungal metabolon coupling tandem inverse-electrondemand Diels-Alder reaction and semipinacol rearrangement for the biosynthesis of spiro polycyclic alkaloids

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Science China Chemistry 2025年第1期68卷 288-296页

作者： Shuai Liu Wen-Qiang Xu Ying-Tong Di Man-Cheng Tang Ding-Kang Chen Ming-Ming Cao Yao-Wen Chang Hong-Yu Tang Chun-Mao Yuan Jun-bo Yang zhi-Li Zuo han Guo Zi-Fei Xu Ying Zeng Yun-Dong Wu Xiao-Jiang Hao Key Laboratory of Phytochemistry and Natural Medicines (Chinese Academy of Sciences) Kunming Institute of Botany Chinese Academy of Sciences University of Chinese Academy of Sciences Lab of Computational Chemistry and Drug Design State Key Laboratory of Chemical Oncogenomics Peking University Shenzhen Graduate School Shenzhen Bay Laboratory State Key Laboratory of Microbial Metabolism School of Life Sciences & Biotechnology Shanghai Jiao Tong University State Key Laboratory of Functions and Applications of Medicinal Plants Guizhou Medical University Molecular Biology Experimental Center Germplasm Bank of Wild Species in Southwest China Kunming Institute of Botany Chinese Academy of Sciences Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences Research Unit of Chemical Biology of Natural Anti-Virus Products Chinese Academy of Medical Sciences

In the intricate process of natural product biosynthesis, a metabolon can enhance metabolic flux by associating sequential enzymes. A fungal metabolon, comprising of flavin-dependent monooxygenase Spe F and P450 monooxygenase Spe G, is identified in the biosynthesis of spiro polycyclic alkaloids(+)-notoamide B and its diastereomer(+)-versicolamide B. Using notoamide E as a substance, Spe F/Spe G metabolon can control the stereoselectivity of its 2,3-epoxidation, followed by hydrogen atom ion at C-17 to generate reactive epoxide tau-m A with dienyl iminium unit. Subsequently,(+)-notoamide B and(+)-versicolamide B are produced via tandem nonenzymatic inverse-electron-demand Diels-Alder reaction and semipinacol rearrangement. This provides the first example of metabolon in the biosynthesis of spiro-prenylated indole alkaloids.

关键词： metabolon Diels-Alder reaction semipinacol rearrangement biosynthesis notoamides spiro polycyclic alkaloids

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材料中氢同位素行为热脱附谱实验方法研究

原子能科学技术 2012年第B9期46卷 510-516页

作者：夏体锐杨洪广占勤韩志博何长水中国原子能科学研究院北京102413

材料中氢同位素行为研究是确保聚变堆安全和经济性的关键问题和重要研究方向。为研究材料中氢同位素的扩散、释放、居留等特性,建立了一种联合四极质谱仪(QMS)的热脱附谱(TDS)实验方法,解决了TDS系统超高真空、低氢同位素质谱本底、线... 详细信息

材料中氢同位素行为研究是确保聚变堆安全和经济性的关键问题和重要研究方向。为研究材料中氢同位素的扩散、释放、居留等特性,建立了一种联合四极质谱仪(QMS)的热脱附谱(TDS)实验方法,解决了TDS系统超高真空、低氢同位素质谱本底、线性升温速率控制以及灵敏度标定等关键科学技术问题。通过涡轮分子泵和二级溅射离子泵实现了优于1×10-7 Pa的超高真空,本底H2分压降至1×10-9 Pa。通过MCGS直流PID控温程序实现样品升温速率在1～100K/min范围可调,采用漏率可变的特制通导型玻璃漏孔标定TDS系统的氘气脱附速率灵敏度,确定该灵敏度系数α和最小可检测氘气热脱附速率(脱附速率灵敏度)分别为6.22×1024 s-1.A-1、1.24×10-10 s-1。采用镀镍Zr-4合金吸氘样品验证了TDS方法的有效性,初步分析了Zr-4中的氘热脱附特性。

关键词：材料氢同位素行为热脱附谱

茂原链轮丝菌转谷氨酰胺酶基因在大肠杆菌中高效表达

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生物工程学报 2005年第5期21卷 794-798页

作者：徐斌韩之波杨萍刘拥军李妍涵韩忠朝中国医学科学院血液学研究所

利用PCR方法从茂原链轮丝菌(Streptoverticilliummobaraense)基因组DNA扩增出微生物转谷氨酰胺酶(MicrobialTransglutaminase,MTG)的基因片段,并构建表达质粒pET_MTG。后者在大肠杆菌(RosettaDE3)中得到高效表达,但表达的MTG存在于包涵体中。经洗涤、变性和复性,并以强阳离子交换层析纯化,获得了SDS_PAGE纯的MTG,并具有与天然酶几乎相同的比活性。此项工作为工业化生产MTG打下了基础。

关键词：微生物转谷氨酰胺酶大肠杆菌表达包涵体

RNA干扰沉默缺氧诱导因子-1α对人乳腺癌细胞功能的影响

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中国医学科学院学报 2006年第5期28卷 670-674页

作者：王晗刘拥军韩之波梁琳慧吕璐璐韩忠朝中国医学科学院中国协和医科大学血液学研究所泰达生命科技研究中心天津300020

目的研究靶向针对缺氧诱导因子-1α(HIF-1α)的短发夹RNA(shRNA)对人乳腺癌细胞系MCF-7细胞功能的影响。方法氯化钴模拟低氧环境。采用RNA干扰技术,构建针对HIF-1α的shRNA真核表达载体,转染MCF-7细胞。RT-PCR检测RNA干扰后MCF-7中血管... 详细信息

目的研究靶向针对缺氧诱导因子-1α(HIF-1α)的短发夹RNA(shRNA)对人乳腺癌细胞系MCF-7细胞功能的影响。方法氯化钴模拟低氧环境。采用RNA干扰技术,构建针对HIF-1α的shRNA真核表达载体,转染MCF-7细胞。RT-PCR检测RNA干扰后MCF-7中血管内皮生长因子(VEGF)表达的变化。实时定量PCR和Westernblot技术分别检测HIF-1αmRNA和蛋白质水平。Sub-G1测定、AnnexinⅤ荧光标记和DNAladder检测细胞凋亡。流式细胞仪检测细胞周期的变化。结果缺氧后,MCF-7中的HIF-1α表达增加(P<0.01),而凋亡率较常氧降低(P<0.05)。shRNA转染MCF-7后,HIF-1α的表达下调91.63%(P<0.01)。阿糖胞苷诱导或无凋亡诱导剂时,干扰组凋亡率比未转染组分别增高1.75倍(P<0.01)和61.31倍(P<0.01)。与未转染组相比,干扰组中的VEGF下调66.8%(P<0.05)。干扰组细胞周期进程也较未转染组减缓。结论HIF-1α在MCF-7中发挥抗细胞凋亡的作用。本室构建的针对HIF-1α的shRNA能够促进MCF-7凋亡,下调VEGF表达,推迟细胞周期。

关键词：缺氧诱导因子-1α 短发夹RNA 人乳腺癌细胞系细胞凋亡血管内皮生长因子细胞周期