检索结果-南通市图书馆

酵母单杂交文库构建及巨峰葡萄VvFT基因启动子上游调控因子筛选

在线全文

学校读者我要写书评

暂无评论

南方农业学报 2020年第12期51卷 2875-2883页

作者：黄桂媛张瑛林玲韩佳宇时晓芳曹雄军郭荣荣广西农业科学院葡萄与葡萄酒研究所南宁530007

【目的】利用酵母单杂交文库筛选巨峰葡萄VvFT基因启动子上游调控因子,挖掘参与调控葡萄开花时间的因子,为揭示花期转变代谢通路和定向改良葡萄品种提供理论参考。【方法】利用PlantCARE分析预测VvFT基因启动子顺式作用元件,并以VvFT基... 详细信息

【目的】利用酵母单杂交文库筛选巨峰葡萄VvFT基因启动子上游调控因子,挖掘参与调控葡萄开花时间的因子,为揭示花期转变代谢通路和定向改良葡萄品种提供理论参考。【方法】利用PlantCARE分析预测VvFT基因启动子顺式作用元件,并以VvFT基因启动子为诱饵,利用酵母单杂交文库筛选技术,筛选作用在VvFT基因启动子上游的调控因子。【结果】葡萄VvFT基因启动子区域存在13种启动子顺式作用元件,包括A-box、CAAT-box和TATA-box组成型调控元件;光响应调控元件ATC-motif、Box 4、G-Box和GT1-motif;茉莉酸甲酯(MeJA)响应调控元件CGTCA-motif和TGACG-motif;干旱诱导的MYB结合位点元件MBS;玉米蛋白代谢必需的调控元件O2-site;参与生物钟控制的调控元件circadian;厌氧诱导必需调控元件ARE。以VvFT基因启动子为诱饵,筛选获得34条表达序列标签(EST),其中有21条为未知功能,有13条在植物生长、抗逆防御、信号转导、转录调控、蛋白酶等方面均有已知或预测的功能,包括转录抑制因子ft41、GRF1互作因子ft44、NAP1相关蛋白ft64及ft70分子伴侣DnaJ10。酵母单杂交点对点验证结果表明VvDnaJ10和VvFT基因启动子之间有相互作用。【结论】通过酵母单杂交文库筛选获取13条候选EST,虽然大部分EST功能预测分析结果与VvFT调控开花时间无明显的直接关系,但研究结果为进一步探索VvFT转录或表达水平控制葡萄开花时间的分子机制提供侯选基因。

关键词：葡萄 VvFT基因启动子酵母单杂交文库调控因子筛选

Extralevator abdominoperineal excision for advanced low rectal cancer:Where to go

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2020年第22期26卷 3012-3023页

作者： yu Tao jia-Gang han Zhen-Jun Wang Department of General Surgery Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China

Since its introduction,extralevator abdominoperineal excision(ELAPE)in the prone position has gained significant attention and recognition as an important surgical procedure for the treatment of advanced low rectal *** studies suggest that because of adequate resection and precise anatomy,ELAPE could decrease the rate of positive circumferential resection margins,intraoperative perforation,and may further decrease local recurrence rate and improve *** studies show that extensive resection of pelvic floor tissue may increase the incidence of wound complications and urogenital ***/robotic ELAPE and trans-perineal minimally invasive approach allow patients to be operated in the lithotomy position,which has advantages of excellent operative view,precise dissection and reduced postoperative *** floor reconstruction with biological mesh could significantly reduce wound complications and the duration of *** proposal of individualized ELAPE could further reduce the occurrence of postoperative urogenital dysfunction and chronic perianal *** ELAPE procedure emphasizes precise anatomy and conforms to the principle of radical resection of tumors,which is a milestone operation for the treatment of advanced low rectal cancer.

关键词： Extralevator abdominoperineal excision Advanced rectal cancer Advantages Complications Pelvic reconstruction Intraoperative position Trans-perineal approach Laparoscopic/robotic-extralevator abdominoperineal excision Individual-extralevator abdominoperineal excision

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

青少年特发性脊柱侧弯治疗的可视化分析

中国组织工程研究 2025年第3期29卷 645-653页

作者：郑晓东高山韩文瑾刘立俊贾梦龙于龙潭山东中医药大学第一临床医学院山东省济南市250199 潍坊市中医院山东省潍坊市261041 西安交通大学医学部陕西省西安市710061

背景:目前脊柱侧弯发病率正在逐年上升,尤以青少年特发性脊柱侧弯最为常见。因此加强对青少年脊柱侧弯治疗的研究就显得越来越重要。目的:旨在总结全球关于青少年特发性脊柱侧弯治疗研究的现状、热点、新兴趋势以及前沿,为今后相关的研... 详细信息

背景:目前脊柱侧弯发病率正在逐年上升,尤以青少年特发性脊柱侧弯最为常见。因此加强对青少年脊柱侧弯治疗的研究就显得越来越重要。目的:旨在总结全球关于青少年特发性脊柱侧弯治疗研究的现状、热点、新兴趋势以及前沿,为今后相关的研究提供参考及指导。方法:从Web of Science核心集数据库中检索2013-2023年有关青少年特发性脊柱侧弯治疗的文献。运用CiteSpace 6.2.R1软件对国家、机构、作者和关键词进行可视化分析。结果与结论:①共纳入561篇英文文献,在国家、机构及作者分析中,美国的贡献最为突出,南京大学及Qiu Yong(南京大学医学院附属鼓楼医院)是发文量最多的机构及作者,发文量最多的学术期刊是《European Spine Journal》。②在被引用文献分析中,引用量前10位的文章主要描述了手术治疗与保守治疗对于改善青少年特发性脊柱侧弯的效果,尤以改善患者曲度为著,③通过对高被引文章进行归纳总结以及对关键词聚类、关键词突现进行深入挖掘发现,目前的研究热点是Cobb角与治疗方法选择的关系、运动疗法的治疗效果与椎体后路融合术的治疗效果。④对于不同曲度的患者,预后情况是否一致未有深入研究,以及青少年特发性脊柱侧弯的病因尚未明确,因此曲率与预后的关系以及青少年特发性脊柱侧弯的病因可能是未来新的研究趋势。

关键词：文献计量学青少年特发性脊柱侧弯治疗 CiteSpace 可视化分析 Cobb角热点趋势

The potential mechanism, recognition and clinical significance of tumor pseudoprogression after immunotherapy

在线全文

学校读者我要写书评

暂无评论

Cancer Biology & Medicine 2019年第4期16卷 655-670页

作者： Wenxiao jia Qianqian Gao Anqin han Hui Zhu Jinming yu Departme nt of Radiati on Oncology Sha ndong Can cer Hospital and In stitute Affiliated to Sha ndong Un iversityJinan 250012China Departme nt of Obstetrics and Gyn ecology Qilu HospitalSha ndong UniversityJinan 250012China Department of Radiati on On cology Sha ndong Can cer Hospital and In stitute Affiliated to Sha ndong UniversitySha ndong Academy of Medical SciencesJinan 250012China

As immunotherapy has gained increasing interest as a new foundation for cancer therapy,some atypical response patterns,such as pseudoprogression and hyperprogression,have garnered the attention of *** is a phenomenon in which an initial increase in tumor size is observed or new lesions appear,followed by a decrease in tumor burden;this phenomenon can benefit patients receiving immunotherapy but often leads to premature discontinuation of treatment owing to the false judgment of *** recognizing pseudoprogression is also a challenge for *** of the extensive attention on pseudoprogression,significant progress has been *** new criteria for immunotherapy,such as irRC,iRECIST and imRECIST,were proposed to accurately evaluate the response to *** new detection indexes,such as ctDNA and IL-8,have also been used to identify *** this review,the definition,evaluation criteria,mechanism,monitoring,management and prognosis of pseudoprogression are summarized,and diagnostic and treatment processes for patients with progression but with a suspicion of pseudoprogression are proposed;these processes could be helpful for physicians in clinical practice and enhances the understanding of pseudoprogression.

关键词： Cancer immunotherapy pseudoprogression RECIST ctDNA IL-8

Numerical simulation of hydrodynamic and water quality effects of shoreline changes in Bohai Bay

在线全文

学校读者我要写书评

暂无评论

Frontiers of Earth Science 2018年第3期12卷 625-639页

作者： han jia 1Yongming SHEN Meirong SU Chunxue yu State Key Laboratory of Coastal and Offshore Engineering Dalian University of Technology Dalian 116024 China School of Environment and Civil Engineering Dongguan University of Technology Dongguan 523808 China

This study uses the HD and Ecolab modules of MIKE to simulate the hydrodynamic and water quality and predict the influence of shoreline changes in Bohai Bay, China. The study shows that shoreline changes weaken the residual current and generate a counter-clockwise circulation south of Huanghua Port, thereby resulting in weak water exchange capacity and low pollutant-diffusing capacity. Shoreline changes reduce the area of Bohai Bay, resulting in a smaller tidal prism and further weakening the water exchange capacity. This situation is not conducive to the diffusion of pollutants, and therefore may lead to increased water pollution in the bay. Shoreline changes hinder the spread of runoff, weaken the dilution effect of the river on seawater, and block the spread of coastal residual Current, thereby resulting in increased salinity near the reclamation area. Shoreline changes lead to an increase in PO4-P concentration and decrease in DIN concentration. The value of N/P near the project decreases, thereby weakening the phosphorus-limited effect.

关键词： Bohai Bay shoreline changes hydrodynamic water quality

不同葡萄砧木品种需冷量及低温时长对芽休眠相关基因表达的影响

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

南方农业学报 2024年第8期55卷 2237-2247页

作者：肖丽冰钟挺威李琳琳陆秀边林钦雄韩佳宇张延晖贾海锋白先进王博广西大学农学院广西南宁530004 广西农业科学院葡萄与葡萄酒研究所广西南宁530007 广西真诚农业有限公司广西南宁530105

【目的】探究不同葡萄砧木品种需冷量及低温时长对芽休眠相关基因表达的影响,为筛选热区葡萄低需冷量砧木品种及探究葡萄萌芽进程调控机制提供理论依据。【方法】以15个葡萄砧木品种的冬季修剪枝梢为试验材料,进行不同低温时长处理,处... 详细信息

【目的】探究不同葡萄砧木品种需冷量及低温时长对芽休眠相关基因表达的影响,为筛选热区葡萄低需冷量砧木品种及探究葡萄萌芽进程调控机制提供理论依据。【方法】以15个葡萄砧木品种的冬季修剪枝梢为试验材料,进行不同低温时长处理,处理温度为(4±1)℃,测定各砧木品种的需冷量,统计各葡萄砧木品种萌芽进程;并研究需冷量有差异的葡萄砧木品种,在低温处理打破休眠过程中枝梢中可溶性总糖和淀粉含量变化;通过实时荧光定量PCR检测砧木芽中休眠相关基因的相对表达量。【结果】贝达、SO4、101-14、110R、188-08、3309C、140R、Valliant、山河2号、BR No.2、Fercal和5C等12个品种需冷量为0 h,1103P需冷量为168 h。1103P砧木枝梢中可溶性总糖含量随着低温处理时长增加呈显著增加趋势(P0.05,下同)。1103P砧木随着低温处理时长增加,胼胝体合成酶基因VvCALLOSE SYNTHASE1的相对表达量呈显著下降趋势,未低温处理的1103P冬芽中VvCALLOSE SYNTHASE1基因的相对表达量显著高于3309C。3个低温时长处理中,1103P芽中β-1,3-葡聚糖酶基因VvGLU1、ABA合成关键酶基因VvNCED1、GA20-氧化酶基因VvGA20ox-2、响应低温胁迫的重要转录因子基因VvbHLH92和开花抑制基因VvSVP的相对表达量在低温处理304 h时最高。未进入内休眠的3309C在低温处理过程中,VvCALLOSE SYNTHASE1和VvSVP基因的相对表达量无显著变化,未经低温处理时的3309C芽中VvGLU1基因、ABA合成诱导因子基因VvXERICO和VvbHLH92基因的相对表达量最高。3个低温时长下1103P芽中VvSVP基因的相对表达量均显著高于3309C。【结论】筛选出贝达、SO4、101-14、110R、188-08、3309C、140R、Valliant、山河2号、BR No.2、Fercal和5C等12个不需经低温处理即可萌芽的砧木品种,葡萄的萌芽受品种需冷量、营养和休眠相关基因的共同调控。

关键词：葡萄砧木需冷量低温处理休眠解除基因表达

维普期刊数据库博看期刊

Micro-arc oxidization fabrication and ethanol sensing performance of Fe-doped TiO_2 thin films

在线全文

学校读者我要写书评

暂无评论

International Journal of Minerals,Metallurgy and Materials 2012年第5期19卷 461-466页

作者： Fu-jian Ren Xiao-bai yu yun-han Ling jia-you Feng Laboratory of Advanced Materials Department of Materials Science and EngineeringTsinghua UniversityBeijing 100084China

In-situ pure TiO2 and Fe-doped TiO2 thin films were synthesized on Ti plates via the micro-arc oxidation （MAO） technique. The as-fabricated anatase TiO2 thin film-based conductometric sensors were employed to measure the gas sensitivity to ethanol. The results showed that Fe ions could be easily introduced into the MAO-TiO2 thin films by adding precursor K4（FeCN）6＇3H20 into the NaaPO4 electrolyte. The amount of doped Fe ions increased almost linearly with the concentration of Kg（FeCN）63H20 increasing, eventually affecting the ethanol sensing performances of TiO2 thin films. It was found that the enhanced sensor signals obtained had an optimal concentration of Fe dopant （1.28at%）, by which the maximal gas sensor signal to 1000 ppm ethanol was estimated to be 7.91 at 275℃. The response time was generally reduced by doped Fe ions, which could be ascribed to the increase of oxygen vacancies caused by Fe3＋ substituting for Ti4＋.

关键词： thin films titanium dioxide doping iron micro-arc oxidation ethanol sensors

Skeletal muscle-specific Bambi deletion induces hypertrophy and oxidative switching coupling with adipocyte thermogenesis against metabolic disorders

在线全文

学校读者我要写书评

暂无评论

Science China Life Sciences 2025年

作者： Xiangping Yao Xudong Mai Ye Tian Yifan Liu Guanghui Jin Ze Li Shujie Chen Xiaoshuang Dai Liujing Huang Zijing Fan Guihua Pan Xiaohan Pan Xiangmin Li Michael C yu jia Sun Jingxing Ou Hong Chen Liwei Xie College of Life and Health Sciences Guangdong Industry Polytechnic Department of Endocrinology and Metabolism Zhujiang Hospital Southern Medical University State Key Laboratory of Applied Microbiology Southern China Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application Guangdong Open Laboratory of Applied MicrobiologyInstitute of Microbiology Guangdong Academy of Sciences Institute of Aging Research Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics School of Medical Technology Guangdong Medical University Department of Hepatic Surgery and Liver transplantation Center of the Third Affiliated Hospital Organ Transplantation Institute Sun Yat-sen University Organ Transplantation Research Center of Guangdong ProvinceGuangdong province engineering laboratory for transplantation medicine Guangdong Provincial Key Laboratory of Liver Disease Research School of Public Health Xinxiang Medical University BGI Institute of Applied Agriculture BGI-Shenzhen Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University Department of Biological Sciences State University of New York at Buffalo Department of Internal Medicine Shunde Women and Children's Hospital (Maternity and Child Healthcare Hospital of Shunde Foshan) Guangdong Medical University

Skeletal muscle plays a significant role in both local and systemic energy metabolism. The current investigation aims to explore the role of the Bambi gene in skeletal muscle, focusing on its implications for muscle hypertrophy and systemic metabolism. We hypothesize that skeletal muscle-specific deletion of Bambi induces muscle hypertrophy, improves metabolic performance, and activates thermogenic adipocytes via the reprogramming of progenitor of i WAT, offering potential therapeutic strategies for metabolic syndromes. Leveraging the Chromatin immunoprecipitation(ChIP)-seq and bioinformatics analysis, Bambi gene is shown to be a direct target of HIF2α, which is further confirmed by ChIP-qPCR and promoter luciferase assay. Skeletal muscle-specific Bambi deletion led to significant muscle hypertrophy and improved metabolic parameters, even under high-fat diet conditions. This deletion induced metabolic reprogramming of stromal vascular fractions(SVFs) into thermogenic adipocytes, contributing to systemic metabolic improvements, potentially through the secretory ***, mice with skeletal muscle-specific Bambi deletion demonstrate resistance to high-fat diet-induced metabolic disorders, highlighting a potential therapeutic pathway for metabolic syndrome management. Thus, skeletal muscle-specific deletion of Bambi triggers muscle growth, enhances metabolic performance, and activates thermogenic adipocytes. These findings suggest Bambi as a novel therapeutic target for metabolic syndromes, providing new insights into the interaction between muscle hypertrophy and systemic metabolic improvement. The study underscores the potential of manipulating muscle physiology to regulate whole-body metabolism, offering a novel perspective on treating metabolic disorders.

关键词：

An Azoester-containing Photoresponsive Linear Liquid Crystal Polymer with Good Mesophase Stability

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2020年第8期38卷 806-813,I0005页

作者： Shu-Qiang han Ying-Ying Chen Bo Xu jia Wei Yan-Lei yu Department of Materials Science and State Key Laboratory of Molecular Engineering of Polymers Fudan UniversityShanghai200433China

Photoresponsive linear liquid crystal polymers(LLCPs)are attractive because of the excellent stimuli-responsibility and the good *** this study,a new photoresponsive LLCP containing azoester(PC11AE6)with good mesophase stability was synthesized by ringopening metathesis *** introducing photoresponsive azoester mesogenic unit,which has high rigidity and a large lengthdiameter ratio,the resultant polymer possesses a broad mesophase temperature interval(isotropic temperature=180℃).A study on mesophase by 2D-wide angle X-ray diffraction indicated that the mesogens were orientated spontaneously into smectic A phase after *** orientated films and fibres exhibited macroscopic,rapid and reversible deformations under light irradiation as a result of the photoisomerization of azoester as confirmed by UV-Vis absorption *** anticipate that this work provides a strategy for preparing LLCP with a broad mesophase temperature range,which is positive for potential applications.

关键词： Ring-opening metathesis polymerization Liquid crystal polymers Azoester Photodeformation