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Metabolic properties of chicken embryonic stem cells

Science China(Life Sciences) 2010年第9期53卷 1073-1084页

作者： LI Jia ZhanG BaoLu han hongbing CAO ZhiCheng LIAN ZhengXing LI Ning State Key Laboratory of Agrobiotechnology China Agricultural UniversityBeijing 100194China College of Animal Science&Technology China Agricultural UniversityBeijing 100194China

Cellular energy metabolism correlates with cell fate,but the metabolic properties of chicken embryonic stem (chES) cells are poorly *** a previously established chES cell model and electron microscopy (EM),we found that undifferentiated chES cells stored ***,undifferentiated chES cells expressed lower levels of glucose transporter 1 (GLUT1) and phosphofructokinase (PFK) mRNAs but higher levels of hexokinase 1 (HK1) and glycogen synthase (GYS) mRNAs compared with control primary chicken embryonic fibroblast (CEF) cells,suggesting that chES cells direct glucose flux towards the glycogenic ***,we demonstrated that undifferentiated chES cells block gluconeogenic outflow and impede the accumulation of glucose-6-phosphate (G6P) from this pathway,as evidenced by the barely detectable levels of pyruvate carboxylase (PCX) and mitochondrial phosphoenolpyruvate carboxykinase (PCK2) ***,cell death occurred in undifferentiated chES cells as shown by Hoechst 33342 and propidium iodide (PI) double staining,but it could be rescued by exogenous ***,we found that differentiated chES cells decreased the glycogen reserve through the use of PAS ***,differentiated chES cells expressed higher levels of GLUT1,HK1 and PFK mRNAs,while the level of GYS mRNA remained similar in control CEF *** data indicate that undifferentiated chES cells continue to synthesize glycogen from glucose at the expense of G6P,while differentiated chES cells have a decreased glycogen reserve,which suggests that the amount of glycogen is indicative of the chES cell state.

关键词： chicken embryonic stem cell energy metabolism glycogen cell fate glucose-6-phosphate

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Four recombinant pluripotency transcriptional factors containing a protein transduction domain maintained the in vitro pluripotency of chicken embryonic stem cells

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Science China(Life Sciences) 2013年第1期56卷 40-50页

作者： YU MiaoYing LIAN Song han hongbing YU Kun LI GuiGuan LIAN ZhengXing LI Ning National Key Laboratory of AgroBiotechnology China Agricultural University Laboratory for Molecular and Cellular Genetics Collage of Animal Science and TechnologyChina Agriculture University

Long-term in vitro maintenance of embryonic stem cell (ESC) pluripotency enables the pluripotency and differentiation of ESCs in animals to be investigated. The ability to successfully maintain and differentiate chicken embryonic stem cells (cESCs) would provide a useful tool for avian biology research and would be a resource directly applicable to agricultural production. In this study, endogenous chicken pluripotency transcription factors, POUV, Sox-2, Nanog and Lin28 were cloned and expressed as recombinant proteins containing a nine consecutive arginine protein transduction domain (PTD). cESCs were cultured with these recombinant proteins to maintain cESC pluripotency in vitro. Cultured cESCs exhibited typical characteristics of pluripotency, even after six generations of rapid doubling, including positive staining for stage-specific embryonic antigen I, and strong staining for alkaline phosphatase. Expression levels of the pluripotency markers, POUV, Nanog, C-Myc, Sox-2 and Lin28 were the same as in uncultured stage X blastoderm cells, and most significantly, the formation of embryoid bodies (EBs) by 6th generation cESCs confirmed the ability of these cultured cESCs to differentiate into cells of all three embryonic germ layers. Thus, transcription factors could be translocated through the cell membrane into the intracellular space of cESCs by using a PTD of nine consecutive arginines and the pluripotency of cESCs could be maintained in vitro for at least six generations.

关键词： recombinant pluripotency factors protein transduction domain chicken ESC pluripotency

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Microbiome and ileum transcriptome revealed the boosting effects of selenium yeast on egg production in aged laying hens

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Animal Nutrition 2022年第3期10卷 124-136页

作者： Zhexi Liu Yutao Cao Yue Ai Xiaonan Yin Linli Wang Mengyao Wang Bingkun Zhang Zhengxing Lian Keliang Wu Yuming Guo hongbing han Beijing Key Laboratory of Animal Genetic Improvement College of Animal Science and TechnologyChina Agricultural UniversityBeijingChina National Engineering Laboratory for Animal Breeding College of Animal Science and TechnologyChina Agricultural UniversityBeijingChina Key Laboratory of Animal Genetics Breeding and Reproduction of the Ministry of Agriculture and Rural AffairsCollege of Animal Science and TechnologyChina Agricultural UniversityBeijingChina Beijing Alltech Biological Products(China)Co. Ltd.BeijingChina State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijingChina

The declines in laying performance during the late productionperiod have adverse effects on the length of theproduction cycle. Improving the nutrition of laying hens is a crucialmeasure to reverse this declination. Thisstudy investigatedthe effect of seleniumyeast (SY)on egg production, ileal gene expressionandmicrobiota, aswell as elucidating their associations in aged laying hens. A total of 375 Jinghong laying hens at 76weeks oldwere randomly assigned into 5 dietary treatments, which included a selenium-deficient basal diet based oncorn-soybean meal, and dietary supplementation of SY at 0.15, 0.30 and 0.45 mg/kg, and sodium selenite at0.45mg/*** results showed that SYamelioratedthe depressionin aged layingperformance inthe 0.30mg/kg group (P han the sodiumselenite group. Functional enrichment andShort Time-series Expression Miner (STEM) analysis indicated that SY activated metabolic progress (e.g.,glycerolipidmetabolism, glycerophospholipidmetabolism, andfattyacidmetabolism),immune responseandoxidative stress response. Four hub genes including thioredoxin reductase 1 (TXNRD1), dihydrolipoamidedehydrogenase (DLD), integrin linked kinase (ILK) and leucine zipper tumor suppressor 2 (LZTS2) wereinvolved in intestinal metabolismwhich was closely associated with selenium deposition/status. Moreover,the relative abundance of Veillonella, Turicibacter and Lactobacillus was significantly increased, but the relativeabundance of Stenotrophomonas was significantly decreased by SY supplementation. Multi-omics dataintegration and Canonical correspondence analysis (CCA) showed that both the ileumselenium content andthe laying rate were highly correlated with pathways and bacteria enriched in metabolism and immuneresponse. Meanwhile, the “switched on” gene prostate stem cell antigen (PSCA) had a positive relationshipwith Veillonella a

关键词： Selenium yeast Egg production Gut microbiota Aged laying hen Transcriptome

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Giving Full Play to the Economic Advantages of the City to Promote the Integrated Development of Beijing-Tianjin-Langfang

Giving Full Play to the Economic Advantages of the City to P...

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对接京津——产业经济行业发展

作者： han hongbing Wuhan University of TechnologyLangfang Bank

Metropolitan economy is a unique economic system that takes municipal administrative divisions as geographical space,which includes urban economy and county economy,with functions of highly concentrated capital,technology,human resources,information and other production factors,and relies on the configuration and combination of these factors to produce the corresponding economic *** present,many urban agglomerations in our country are actively exploring the idea of regional economic integration and implementing the process of regional economic integration,which have achieved remarkable *** article analyzes the above contents.

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Research on the Development of Regional Economy by Industrial Cluster

Research on the Development of Regional Economy by Industria...

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对接京津——产业经济行业发展

作者： han hongbing Wuhan University of TechnologyLangfang Bank

Industrial cluster is an important source of regional competitiveness and a powerful carrier of regional economic *** the basis of reviewing and commenting on regional development theory and industrial cluster theory,the paper analyzes the relationship between *** holds that industrial cluster plays an irreplaceable role in the development of regional economy,which can be said to be the only way for regional economic development,and industrial cluster policy is a strategic choice of regional economic development.

关键词： industrialclustertheory regionaleconomicdevelopment relationshipanalysis

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Melatonin improves the first cleavage of parthenogenetic embryos from vitrified-warmed mouse oocytes potentially by promoting cell cycle progression

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Journal of Animal Science and Biotechnology 2022年第1期13卷 106-122页

作者： Bo Pan Izhar Hyder Qazi Shichao Guo Jingyu Yang Jianpeng Qin Tianyi Lv Shengqin Zang Yan Zhang Changjun Zeng Qingyong Meng hongbing han Guangbin Zhou Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province College of Animal Science and TechnologySichuan Agricultural UniversityChengdu 611130China Department of Veterinary Anatomy and Histology Shaheed Benazir Bhutto University of Veterinary and Animal SciencesSakrandSindh 67210Pakistan State Key Laboratory of AgroBiotechnology China Agricultural UniversityBeijing 100193China National Engineering Laboratory for Animal Breeding Key Laboratory of Animal Genetics and Breeding of the Ministry of AgricultureBeijing Key Laboratory for Animal Genetic ImprovementCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Background:This study investigated the effect of melatonin(MT)on cell cycle(G1/S/G2/M)of parthenogenetic zygotes developed from vitrified-warmed mouse metaphase II(MII)oocytes and elucidated the potential mechanism of MT action in the first cleavage of ***:After vitrification and warming,oocytes were parthenogenetically activated(PA)and in vitro cultured(IVC).Then the spindle morphology and chromosome segregation in oocytes,the maternal mRNA levels of genes including Miss,Doc1r,Setd2 and Ythdf2 in activated oocytes,pronuclear formation,the S phase duration in zygotes,mitochondrial function at G1 phase,reactive oxygen species(ROS)level at S phase,DNA damage at G2 phase,early apoptosis in 2-cell embryos,cleavage and blastocyst formation rates were *** results indicated that the vitrification/warming procedures led to following perturbations 1)spindle abnormalities and chromosome misalignment,alteration of maternal mRNAs and delay in pronucleus formation,2)decreased mitochondrial membrane potential(MMP)and lower adenosine triphosphate(ATP)levels,increased ROS production and DNA damage,G1/S and S/G2 phase transition delay,and delayed first cleavage,and 3)increased early apoptosis and lower levels of cleavage and blastocyst *** results further revealed that such negative impacts of oocyte cryopreservation could be alleviated by supplementation of warming,recovery,PA and IVC media with 10^(−9) mol/L MT before the embryos moved into the 2-cell stage of ***:MT might promote cell cycle progression via regulation of MMP,ATP,ROS and maternal mRNA levels,potentially increasing the first cleavage of parthenogenetic zygotes developed from vitrified-warmed mouse oocytes and their subsequent development.

关键词： Cell cycle Cleavage rate Melatonin MetaphaseⅡoocyte Parthenogenetic activation Vitrification

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Organic polaritonic light-emitting diodes with high luminance and color purity toward laser displays

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Light(Science & Applications) 2024年第9期13卷 1956-1963页

作者： Jianbo De Ruiyang Zhao Fan Yin Chunling Gu Teng Long han Huang Xue Cao Cunbin An Bo Liao hongbing Fu Qing Liao Beijing Key Laboratory for Optical Materials and Photonic Devices Department of ChemistryCapital Normal University100048 BeijingChina Beijing Special Engineering Design and Research Institute 100028 BeijingChina Institute of Process Engineering Chinese Academy of Sciences100190 BeijingChina Institute of Molecule Plus Tianjin University and Collaborative Innovation Center of Chemical Science and Engineering(Tianjin)300072 TianjinChina School of Materials Science and Engineering Hunan University of Science and Technology411201 XiangtanHunanChina

Achieving high-luminescence organic light-emitting devices(OLEDs)with narrowband emission and high color purity is important in various optoelectronic *** displays exhibit outstanding advantages in next-generation display technologies owing to their ultimate visual experience,but this remains a great ***,we develop a novel OLED based organic single *** strongly coupling the organic exciton state to an optical microcavity,we obtain polariton electroluminescent(EL)emission from the polariton OLEDs(OPLEDs)with high luminance,narrow-band emission,high color purity,high polarization as well as excellent optically pumped polariton ***,we evaluate the potential for electrically pumped polariton laser through theoretical analysis and provide possible *** work provides a powerful strategy with a material–device combination that paves the way for electrically driven organic single-crystal-based polariton luminescent devices and possibly lasers.

关键词： pumped laser polarization

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Early warning of core network capacity in space-terrestrial integrated networks

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Journal of Systems Engineering and Electronics 2024年第4期35卷 855-864页

作者： han Sai LI Ao ZhanG Dongyue ZHU Bin WANG Zelin WANG Guangquan MIAO Jie MA hongbing China Unicom Research Institute Beijing 100044China China United Network Communications Group Company Limited Beijing 100033China

With the rapid development of low-orbit satellite com-munication networks both domestically and internationally,space-terrestrial integrated networks will become the future development *** space and terrestrial networks with limi-ted resources,the utilization efficiency of the entire space-terres-trial integrated networks resources can be affected by the core network *** order to improve the response efficiency of core networks expansion construction,early warning of the core network elements capacity is *** on the inte-grated architecture of space and terrestrial network,multidimen-sional factors are considered in this paper,including the number of terminals,login users,and the rules of users’migration during *** artifical intelligence(AI)technologies,the regis-tered users of the access and mobility management function(AMF),authorization users of the unified data management(UDM),protocol data unit(PDU)sessions of session manage-ment function(SMF)are predicted in combination with the num-ber of login users,the number of ***,the core network elements capacity can be predicted in *** proposed method is proven to be effective based on the data from real network.

关键词： space-terrestrial integrated networks core network element capacity artificial intelligent(AI)

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澳洲白羊生长和繁殖性状的遗传评估

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中国畜禽种业 2024年第4期20卷 85-97页

作者：朱亚宁秦浩张清峰林春建郑文新吴伟伟韩红兵禽畜遗传改良北京市重点实验室/中国农业大学动物科技学院北京100193 农业与农村部动物遗传育种与繁育重点实验室/中国农业大学动物科学技术学院北京100193 天津奥群牧业有限公司天津301607 新疆畜牧科学院新疆乌鲁木齐830011

该研究旨在评估澳洲白羊的生长和繁殖性状的遗传特性,为科学选育、良种扩繁和提高优良特性提供理论基础。在对生长性状(初生重、 90天体重、 180天体重、 240天体重、 0~90 d日增重、 90~240 d日增重)和繁殖性状(头胎产羔数、经产羔羊... 详细信息

该研究旨在评估澳洲白羊的生长和繁殖性状的遗传特性,为科学选育、良种扩繁和提高优良特性提供理论基础。在对生长性状(初生重、 90天体重、 180天体重、 240天体重、 0~90 d日增重、 90~240 d日增重)和繁殖性状(头胎产羔数、经产羔羊数、头胎窝均重、经产窝均重、头胎窝总重、经产窝总重)进行遗传评估时,使用R包AEFchida建立动物线性混合模型,进行方差组分、遗传参数、遗传相关性和育种值的估计;对于初生重,根据不同母体效应,运用R(v4-1-1)包AEFchida,比较了6种随机效应不同的动物线性混合模型。结果表明,考虑个体加性效应、母体环境效应和母体遗传效应的模型BW1为最优模型,计算初生重遗传力为(0.33±0.04)。繁殖性状在0.03~0.12为低遗传力,其中活羔数(头胎、经产)的遗传力在0.03~0.04,而生长性状在0.33~0.77之间为高遗传力,其中日增重(0~90日、 90~240日)的遗传力在0.46~0.67。产羔数与生长性状相关性较低(0~0.25),初生重与生长性状相关性适中(0.14~0.64),且表型相关性和遗传相关性基本一致。生长性状的育种均值随着年份的增加,整体呈上升趋势;繁殖性状的育种均值则变化幅度较小,选育效果不明显。可见,澳洲白羊繁殖性状和生长性状遗传特性相差较大,应采用不同的选育方式来进一步优化澳洲白羊的生产性能和遗传特性。

关键词：澳洲白羊生长性状繁殖性状初生重随机模型遗传评估

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Efficient production of pronuclear embryos in breeding and nonbreeding season for generating transgenic sheep overexpressing TLR4

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Journal of Animal Science and Biotechnology 2017年第1期8卷 43-50页

作者： Yan Li Di Lian Shoulong Deng Xiaosheng Zhang Jinlong Zhang Wenting Li Hai Bai Zhixian Wang Hongping Wu Juncai Fu hongbing han Jianzhong Feng Guoshi Liu Ling Lian Zhengxing Lian Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture Beijing Key Laboratory for Animal Genetic Improvement College of Animal Science and Technology China Agricultural University Beijing 100193 China Department of Public Health Benedictine University Lisle IL 60532 USA State Key Laboratory of Reproductive Biology institute of Zoology Chinese Academy of Sciences Beijing 100101 China Tianjin Institute of Animal Sciences Tianjin 300381 China

Background： Brucella is a zoonotic Gram-negative pathogen that causes abortion and infertility in ruminants and humans. TLR4 is the receptor for LPS which can recognize Brucella and initiate antigen-presenting cell activities that affect both innate and adaptive immunity. Consequently, transgenic sheep over-expressing TLR4 are an suitable model to investigate the effects of TLR4 on preventing Brucellosis. In this study, we generated transgenic sheep overexpressing TLR4 and aimed to evaluate the effects of different seasons（breeding and non-breeding season） on superovulation and the imported exogenous gene on ***： In total of 43 donor ewes and 166 recipient ewes in breeding season, 37 donor ewes and 144 recipient ewes in non-breeding season were selected for super-ovulation and injected embryo transfer to generate transgenic *** results indicated the no. of embryos recovered of donors and the rate of pronuclear embryos did not show any significant difference between breeding and non-breeding seasons（P 〉 0.05）. The positive rate of exogenous TLR4 tested were 21.21 % and 22.58 % in breeding and non-breeding season by Southern blot. The expression level of TLR4 in the transgenic sheep was 1.5 times higher than in the non-transgenic group（P 〈 0.05）. The lambs overexpressing TLR4 had similar growth performance with non-transgenic lambs, and the blood physiological parameters of transgenic and non-transgenic were both in the normal range and did not show any ***： Here we establish an efficient platform for the production of transgenic sheep by the microinjection of pronuclear embryos during the whole year. The over-expression of TLR4 had no adverse effect on the growth of the sheep.

关键词： Growth Microinjection Pronuclear embryos Sheep Superovulation

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