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Electro-oxidation of Ascorbic Acid on PVP-stabilized Graphene Electrode

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Chemical Research in Chinese Universities 2010年第2期26卷 287-290页

作者： han dong-fang Shan Chang-sheng GUO Li-ping NIU Li han dong-xue State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 P. R. China Department of Chemistry Northeast Normal University Changchun 130024 P. R. China

Polyvinylpyrrolidone-stabilized graphene（PVP-graphene） was synthesized and investigated as a modifier for the determination of ascorbic acid（AA）. With PVP acting as stabilizer and dispersant, the resulting PVP-graphene material could disperse well into water. And the PVP-graphene modified glassy carbon electrode（PVP-graphene-GCE） showed an obvious electrocatalytical activity toward the oxidation of AA in a phosphate buffer solution（PBS, pH=7.0） with an oxidation potential of AA at 0.052 V vs. AglAgCl（sat. KCl）. The calibration curve for APt was linear in a concentration range from 1.0×10^-5 to 5.0×10^-4 mol/L with a correlation coefficient of 0.9998. And the detection limit was found to be 1 μtmol/L. During the oxidation of AA, the π-π interaction of graphene plane with conjugated hexenoic aeid-lactone in AA molecules might play a key role. As a result, an obvious decrease of overpotential was achieved at such a PVP-graphene electrode through a possible adsorption/enrichment process, which will probably trigger potential applications for the electroanalysis of some aromatic and heterocyclic compounds.

关键词： Graphene Polyvinylpyrrolidone Electro-oxidation Ascorbic acid

Charge Transport Processes of Immobilized Heteropolyanions at Self-assembled Monolayer Modified Gold Electrode by Electrochemical Quartz Crystal Microbalance Measurement

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Chemical Research in Chinese Universities 2009年第4期25卷 537-542页

作者： BU Xin-yuan JIAO Lian-sheng han dong-xue YANG Gui-fu State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 P. R. China School of Computer Science Northeast Normal University Changchun 130024 P. R. China

The in situ electrochemical quartz crystal microbalance（EQCM） technique was used to investigate the ion transport of immobilized heteropolyanions at a self-assembled monolayer（SAM） modified gold electrode during electrochemical redox process.A mixed transfer method was presented to analyse the abnormal change of resonant frequency based on the simultaneous insertion/extraction of different ions.The results indicate that the migration of HSO4-anions was indispensable in the redox process of the heteropolyanions in a 1 mol/L H2SO4 solution and played a key role in the abnormal change of the resonant frequency.Such a change was attributed to different packing densities derived by means of differently immobilized methods.

关键词： Heteropolyanion Charge transport Self-assembled monolayer Electrochemical quartz crystal microbalance

Self-assembling Process of Alkanethiol Monolayers on Gold Surface via Underpotential Deposition

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Chemical Research in Chinese Universities 2006年第6期22卷 783-786页

作者： han dong-xue XU Xiao-yu HOU Peng YANG Gui-fu NIU Li ZHAO Jian-wei State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 P. R. China College of Chemistry Jilin University Changchun 130021 P. R. China Department of Chemistry Nanjing University Nanjing 210093 P. R. China

It was demonstrated feasible that underpotential deposition（UPD） of copper on a monolayer-modified gold substrate can be used to determine the gold electrode area. The deposition and stripping of a Cu adlayer can take place reversibly and stably at a bared or a self-assembled monolayer modified gold electrode. The growth kinetics of decanethiol/Au was also investigated v/a Cu UPD. The difference between the assembling kinetics determined by UPD and that by quartz crystal microbalance measurements reveals the configuration transmutation of the assembled molecules from a disordered arrangement to an ordered arrangement durinr the self-assembling processes.

关键词： SAM Configuration transmutation UPD QCM

Determination of Amino Acids by Ru(bpy)_3^(2+) Cathodic Electrochemiluminescence with High Sensitivity

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Chemical Research in Chinese Universities 2010年第3期26卷 376-379页

作者： ZHOU Xiao-guang YUAN Bai-qing ZhanG Lei LIU Yang han dong-xue YOU Tian-yan Department of Chemistry and Chemical Engineering College of Science Northeast Forestry University Harbin 150040 P. R. China State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changehun 130022 P. R. China College of Chemistry Jilin University Changchun 130021 P. R. China

The unique cathodic electrochemiluminescence（ECL） emission of Ru（bpy）3^2＋（bpy=2,2′-bipyridine） was observed via Nation film at Au electrode[Au/Nafion/Ru（bpy）3^2＋] at about 0.20 V（vs. Ag/AgCl） and applied to the determination of several amino acids without prior derivatization with high sensitivity. The cathodic electrochemiluminescence（ECL） exhibits the detection limits and linear ranges of several amino acids comparable to or better than those of capillary electrophoresis with conventional ECL detection method（at 1.10-1.20 V vs. Ag/AgCl） based on precolumn derivatization. The results suggest that the cathodic ECL is promising for the detection of amino acids in bioanalysis.

关键词： Cathodic electrochemiluminescence Ru（bpy）3^2＋ Nation Au electrode

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基于核酸适体的电化学生物传感研究

分析测试学报 2024年第2期43卷 328-337页

作者：李诗琪骆怡琳万建文胡琼韩冬雪牛利广州大学化学化工学院广州大学分析科学技术研究中心&广州市传感材料与器件重点实验室&广东省光电传感材料与器件工程技术研究中心广东广州510006 中山大学化学工程与技术学院广东珠海519082

核酸适体是一类经由指数富集的配体系统进化(SELEX)技术在体外筛选获得的单链寡核苷酸片段,由于具有可人工批量合成、价格低廉、易于功能化修饰、特异性强、亲和力高、免疫原性低、批间差异小、热稳定性好等优良特性,在分析化学、疾病... 详细信息

核酸适体是一类经由指数富集的配体系统进化(SELEX)技术在体外筛选获得的单链寡核苷酸片段,由于具有可人工批量合成、价格低廉、易于功能化修饰、特异性强、亲和力高、免疫原性低、批间差异小、热稳定性好等优良特性,在分析化学、疾病治疗以及生物医学研究等诸多领域备受关注。结合代表性案例,该文综述了核酸适体在电化学生物传感领域的应用。首先简要概述了核酸适体及电化学适体传感器的特点,分类阐述了电化学适体传感器在小分子化合物、蛋白质、外泌体、循环肿瘤细胞(CTCs)以及病原微生物检测中的应用,并重点介绍了相关检测方法的原理、分析特性以及所应用的信号放大策略,最后对电化学适体传感器的发展进行了展望。

关键词：电化学生物传感适体传感器生物标志物外泌体循环肿瘤细胞病原微生物信号放大

三阴性乳腺癌改良根治术后应用白蛋白结合型紫杉醇化疗的长期预后及安全性分析

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哈尔滨医科大学学报 2024年第1期58卷 31-35页

作者：李硕刘雨亭刘霜月韩学东淮安市第一人民医院甲乳外科淮安江苏223000

目的分析三阴性乳腺癌改良根治术后应用白蛋白结合型紫杉醇化疗的效果及安全性。方法选择2017年3月~2022年2月医院收治的98例三阴性乳腺癌改良根治术后患者,以随机数字表法分为对照组(n=49)、研究组(n=49)。对照组术后接受表柔比星+... 详细信息

目的分析三阴性乳腺癌改良根治术后应用白蛋白结合型紫杉醇化疗的效果及安全性。方法选择2017年3月~2022年2月医院收治的98例三阴性乳腺癌改良根治术后患者,以随机数字表法分为对照组(n=49)、研究组(n=49)。对照组术后接受表柔比星+环磷酰胺+溶剂型紫杉醇方案化疗,研究组术后接受表柔比星+环磷酰胺+白蛋白结合型紫杉醇方案化疗。21 d为1个周期,2组均化疗3个周期。比较2组卡氏功能状态(Karnofsky performance status, KPS)评分、生活质量、肿瘤标志物水平、不良反应。随访5年,比较2组生存情况。结果化疗后,2组KPS评分、生活质量健康调查简表(the MOS item short from health survey, SF-36)评分均升高(P0.05),研究组Ⅰ~Ⅳ级白细胞减少、神经毒性总发生率低于对照组(P0.05)。结论三阴性乳腺癌改良根治术后应用白蛋白结合型紫杉醇化疗可降低肿瘤标志物水平,改善生活质量,长期预后良好,且安全可靠。

关键词：三阴性乳腺癌改良根治术白蛋白结合型紫杉醇化疗安全性长期预后

Biomimetic design of highly flexible metal oxide nanofibrous membranes with exceptional mechanical performance for superior phosphopeptide enrichment

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Nano Research 2024年第5期17卷 4270-4278页

作者： xue Mao Zhen-Zhen Li dong-Lian Hao Wei-dong han Gao-Peng Li Yao-Yu Wang Kun Zhang School of Textile Science and Engineering Key Laboratory of Functional Textile Material and Product(Ministry of Education)Xi’an Polytechnic UniversityXi’an 710048China Key Laboratory of Magnetic Molecules&Magnetic Information Materials of the Ministry of Education School of Chemistry&Material ScienceShanxi Normal UniversityTaiyuan 030031China Department of Clinical Laboratory The Second Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710004China Shanghai iProteome Biotechnology Co. LtdShanghai 201210China College of Chemistry&Materials Science Northwest UniversityXi'an 710127China

Developing free-standing and mechanical robust membrane materials capable of superior enrichment of phosphopeptides for analyzing and identifying the specific phosphoproteome of cancer cells is significant in understanding the molecular mechanisms of cancer development and exploring new therapeutic approaches,but still a significant challenge in materials design.To this end,we firstly constructed highly flexible ZrTiO_(4) nanofibrous membranes(NFMs)with excellent mechanical stability through a cost-effective and scalable electrospinning and subsequent calcination technique.Then,to further increase the enrichment capacity of the phosphopeptide,the biomimetic TiO_(2)@ZrTiO_(4) NFMs with root hair or leaf like branch microstructure are developed by the hydrothermal post-synthetic modification of ZrTiO_(4) NFMs through growing unfurling TiO_(2) nanosheets onto the ZrTiO_(4) nanofibers.Importantly,remarkable flexibility and mechanical stability enable the resulting TiO_(2)@ZrTiO_(4) NFMs excellent practicability,while the biomimetic microstructure allows it outstanding enrichment ability of the phosphopeptide and identification ability of the specific phosphoproteins in the digest of cervical cancer cells.Specifically,6770 phosphopeptides can be enriched by TiO_(2)@ZrTiO_(4) NFMs(2205 corresponding phosphoproteins can be identified),and the value is much higher than that of ZrTiO_(4) NFMs(6399 phosphopeptides and 2132 identified phosphoproteins)and commercial high-performance TiO_(2) particles(4525 phosphopeptides and 1811 identified phosphoproteins).These results demonstrate the super ability of TiO_(2)@ZrTiO_(4) NFMs in phosphopeptide enrichment and great potential for exploring the pathogenesis of cancer.

关键词： inorganic nanofibers flexible ceramic nanofibrous membranes biomimetic materials exceptional mechanical performance flexibility

Mutations in the SARS-CoV-2 spike receptor binding domain and their delicate balance between ACE2 affinity and antibody evasion

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Protein & Cell 2024年第6期15卷 403-418页

作者： Song xue Yuru han Fan Wu Qiao Wang Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS) Shanghai Institute of Infectious Disease and BiosecurityShanghai Frontiers Science Center of Pathogenic Microorganisms and InfectionSchool of Basic Medical SciencesShanghai Medical CollegeFudan UniversityShanghai 200032China

Intensive selection pressure constrains the evolutionary trajectory of SARS-CoV-2 genomes and results in various novel variants with distinct mutation profiles.Point mutations,particularly those within the receptor binding domain(RBD)of SARS-CoV-2 spike(S)protein,lead to the functional alteration in both receptor engagement and monoclonal antibody(mAb)recognition.Here,we review the data of the RBD point mutations possessed by major SARS-CoV-2 variants and discuss their individual effects on ACE2 affinity and immune evasion.Many single amino acid substitutions within RBD epitopes crucial for the antibody evasion capacity may conversely weaken ACE2 binding affinity.However,this weakened effect could be largely compensated by specific epistatic mutations,such as N501Y,thus maintaining the overall ACE2 affinity for the spike protein of all major variants.The predominant direction of SARS-CoV-2 evolution lies neither in promoting ACE2 affinity nor evading mAb neutralization but in maintaining a delicate balance between these two dimensions.Together,this review interprets how RBD mutations efficiently resist antibody neutralization and meanwhile how the affinity between ACE2 and spike protein is maintained,emphasizing the significance of comprehensive assessment of spike mutations.

关键词： ACE2 affinity maintained

Panoramic variation analysis of a family with neurodevelopmental disorders caused by biallelic loss-of-function variants in TMEM141,DDHD2,and LHFPL5

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Frontiers of Medicine 2024年第1期18卷 81-97页

作者： Liwei Sun xueting Yang Amjad Khan xue Yu han Zhang Shirui han Xiaerbati Habulieti Yang Sun Rongrong Wang xue Zhang McKusick-Zhang Center for Genetic Medicine State Key Laboratory for Complex Severe and Rare DiseasesInstitute of Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical CollegeBeijing100005China Chongqing Key Laboratory of Human Embryo Engineering Center for Reproductive MedicineNational Key Clinical Speciality Construction Project(Obstetrics and Gynecology)Chongqing Health Center for Women and ChildrenChongqing400013China Chongqing Clinical Research Center for Reproductive Medicine Women and Children’s Hospital of Chongqing Medical UniversityChongqing400013China Faculty of Biological Sciences Department of ZoologyUniversity of Lakki MarwatKhyber Pakhtunkhwa28420Pakistan Institute for Medical Genetics and Applied Genomics University of TübingenTübingen72076Germany Alexander von Humboldt fellowship Foundation Berlin10117Germany Department of Pediatrics The First Affiliated Hospital of Guangxi Medical UniversityNanning530000China Department of Laboratory Medicine State Key Laboratory for Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Science and Peking Union Medical CollegeBeijing100730China

Highly clinical and genetic heterogeneity of neurodevelopmental disorders presents a major challenge in clinical genetics and medicine.Panoramic variation analysis is imperative to analyze the disease phenotypes resulting from multilocus genomic variation.Here,a Pakistani family with parental consanguinity was presented,characterized with severe intellectual disability(ID),spastic paraplegia,and deafness.Homozygosity mapping,integrated single nucleotide polymorphism(SNP)array,whole-exome sequencing,and whole-genome sequencing were performed,and homozygous variants in TMEM141(c.270G>A,p.Trp90^(*)),DDHD2(c.411+767_c.1249-327del),and LHFPL5(c.250delC,p.Leu84^(*))were identified.A Tmem141^(p.Trp90^(*)/p.Trp90^(*))mouse model was generated.Behavioral studies showed impairments in learning ability and motor coordination.Brain slice electrophysiology and Golgi staining demonstrated deficient synaptic plasticity in hippocampal neurons and abnormal dendritic branching in cerebellar Purkinje cells.Transmission electron microscopy showed abnormal mitochondrial morphology.Furthermore,studies on a human in vitro neuronal model(SH-SY5Y cells)with stable shRNA-mediated knockdown of TMEM141 showed deleterious effect on bioenergetic function,possibly explaining the pathogenesis of replicated phenotypes in the cross-species mouse model.Conclusively,panoramic variation analysis revealed that multilocus genomic variations of TMEM141,DDHD2,and LHFPL5 together caused variable phenotypes in patient.Notably,the biallelic loss-of-function variants of TMEM141 were responsible for syndromic ID.

关键词： neurodevelopmental disorder autosomal recessive intellectual disability consanguinity spastic paraplegia hearing loss TMEM141