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Exogenous artificial dNA forms chromatin structure with active transcription in yeast

Science China(Life Sciences) 2022年第5期65卷 851-860页

作者： Jianting zhou Chao zhang Ran wei Mingzhe han Songduo wang Kaiguang yang Lihua zhang weigang Chen Mingzhang wen Cheng Li wei tao Ying-Jin Yuan Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education) Tianjin UniversityTianjin 300072China SynBio Research platform Collaborative Innovation Center of Chemical Science and Engineering(Tianjin)School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China The MOE Key Laboratory of Cell proliferation and differentiation School of Life SciencesPeking UniversityBeijing 100871China CAS Key Laboratory of Separation Science for Analytical Chemistry National Chromatographic R.&A.CenterDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China University of Chinese Academy of Sciences Beijing 100049China School of Microelectronics Tianjin UniversityTianjin 300072China

Yeast artificial chromosomes(YACs) are important tools for sequencing,gene cloning,and transferring large quantities of genetic ***,the structure and activity of YAC chromatin,as well as the unintended impacts of introducing foreign dNA sequences on dNA-associated biochemical events,have not been widely ***,we sh.w.d that abundant genetic elements like TATA box and transcription factor-binding motifs occurred unintentionally in a previously reported datacarrying chromosome(d Chr).In addition,we used state-of-the-art sequencing technologies to comprehensively profile the genetic,epigenetic,transcriptional,and.p.oteomic characteristics of the exogenous d *** found that the data-carrying dNA formed active chromatin with high chromatin accessibility and h3K4 tri-methylation *** d Chr also displayed highly pervasive transcriptional ability and transcribed hundreds of noncoding *** results demonstrated that exogenous artificial chromosomes formed chromatin structures and did not remain as naked or loose plasmids.A better understanding of the YAC chromatin nature will improve our ability to design better data-storage chromosomes.

关键词： yeast artificial chromosome dNA storage epigenetics chromatin accessibility histone h3K4 tri-methylation

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A mixed-valence polyoxometalate-based 3d inorganic framework cathode material for high-efficiency rechargeable AzIBs

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Rare Metals 2024年第8期43卷 3677-3691页

作者： Qing han hao-Ran Xiao tao zhou Bing-Chuan Li Liu yang Ling-Ling Xie Xue-Jing Qiu Xian-Yong wu Li-Min zhu Xiao-Yu Cao School of Chemistry and Chemical Engineering Henan University of TechnologyZhengzhou 450001China Key Laboratory of high Specific Energy Materials for Electrochemical power Sources of zhengzhou City Zhengzhou 450001China College of Materials Engineering Henan University of EngineeringZhengzhou450007China School of Environmental Engineering Henan University of TechnologyZhengzhou 450001China d.p.rtment of Chemistry University of Puerto Rico-Rio Piedras CampusSan JuanPR 00925USA

The global trend towards new energy storage systems has stimulated the development of electrochemical energy storage *** these technologies,rechargeable aqueous zinc-ion batteries(AzIBs)have attracted considerable interest as a potential alternative to lithium-ion batteries(LIBs)due to their affordable cost,environmental compatibility and high safety *** this study,a high-quality electrode for AzIBs has been successfully developed using a dehydrated mixed-valence polyoxometalate-based three-dimensional(3d)inorganic framework material known as[h_6Mn_(3)V^Ⅳ_(15)V-^Ⅴ_(4O)_(46)(h_2O)_(12)](3d-MnVO).This innovative 3d-MnVO material is built from the alternate connections of{V_(19)O_(46)}"sphere-shaped"clusters andμ_(2)-{Mn(h_(2)O)_(4)}bridges,where each{V_(19)O_(46)}cluster is surrounded by three pairs of vertically distributed{Mn(h_(2)O)_(4)}units,thus resulting in the 3d interpenetrating grid-like network from the infinite[-{V_(19)O_(46)}-μ_(2)-Mn(h_(2)O)_(4)-{V_(19)O_(46)}]_∞chains in three mutually perpendicular *** 3d framework structure of 3d-MnVO possesses abundant oxygen vacancies,spacious and multi-level interconnected channels for ion transport,which facilitates the efficient intercalation/deintercalation of hydrated zn^(2+)into the pores of the primary structure via the intercalation capacitance *** a result,the 3d-MnVO electrode exhibits excellent diffusion rates and minimal interfacial *** a current density of 0.1 A·g^(-1),the 3d-MnVO cathode delivers a commendable discharge capacity of170.5 mAh·g^(-1)with 81.6%capacity retention after100 charge/discharge ***,even at a high current density of 1.0 A·g^(-1),the 3d-MnVO electrode delivers a remarkable reversible capacity of198.9 mAh·g^(-1).Our research results provide valuable insights into the development of advanced.p.lyoxometalate-based 3d inorganic framework electrode materials for high-performance rechargeable AzIBs.

关键词： Cathode polyoxometalate 3d inorganic framework zinc-ion batteries Energy storage mechanism

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Measurements of the center-of-mass energies of e^(+)e^(-)collisions at BESIII

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Chinese physics C 2021年第10期45卷 7-15页

作者： M.Ablikim M.N.Achasov p.Adlarson S.Ahmed M.Albrecht R.Aliberti A.Amoroso M.R.An Q.An X.h.Bai Y.Bai O.Bakina R.Baldini Ferroli I.Balossino Y.Ban K.Begzsuren N.Berger M.Bertani d.Bettoni F.Bianchi J.Bloms A.Bortone I.Boyko R.A.Briere h.Cai X.Cai A.Calcaterra G.F.Cao N.Cao S.A.Cetin J.F.Chang w.L.Chang G.Chelkov d.Y.Chen G.Chen h.S.Chen M.L.Chen S.J.Chen X.R.Chen Y.B.Chen z.J.Chen w.S.Cheng G.Cibinetto F.Cossio X.F.Cui h.L.dai X.C.dai A.dbeyssi R.E.de Boer d.dedovich z.Y.deng A.denig I.denysenko M.destefanis F.de Mori Y.ding C.dong J.dong L.Y.dong M.Y.dong X.dong S.X.du Y.L.Fan J.Fang S.S.Fang Y.Fang R.Farinelli L.Fava F.Feldbauer G.Felici C.Q.Feng J.h.Feng M.Fritsch C.d.Fu Y.Gao Y.Gao Y.Gao Y.G.Gao I.Garzia p.T.Ge C.Geng E.M.Gersabeck A Gilman K.Goetzen L.Gong w.X.Gong w.Gradl M.Greco L.M.Gu M.h.Gu Y.T.Gu C.Y Guan A.Q.Guo L.B.Guo R.p.Guo Y.p.Guo A.Guskov T.T.han w.Y.han X.Q.hao F.A.harris K.L.he F.h.heinsius C.h.heinz T.held Y.K.heng C.herold M.himmelreich T.holtmann G.Y.hou Y.R.hou z.L.hou h.M.hu J.F.hu T.hu Y.hu G.S.huang L.Q.huang X.T.huang Y.p.huang z.h.ang T.hussain N husken w.Ikegami Andersson w.Imoehl M.Irshad S.Jaeger S.Janchiv Q.Ji Q.p.Ji X.B.Ji X.L.Ji Y.Y.Ji h.B.Jiang X.S.Jiang J.B.Jiao z.Jiao S.Jin Y.Jin M.Q.Jing T.Johansson N.Kalantar-Nayestanaki X.S.Kang R.Kappert M.Kavatsyuk B.C.Ke I.K.Keshk A.Khoukaz p.Kiese R.Kiuchi R.Kliemt L.Koch O.B.Kolcu B.Kopf M.Kuemmel M.Kuessner A.Kupsc M.G.Kurth.w.Kuhn J.J.Lane J.S.Lange p.Larin A.Lavania L.Lavezzi z.h.Lei h.Leithoff M.Lellmann T.Lenz C.Li C.h.Li Cheng Li d.M.Li F.Li G.Li h.Li h.Li h.B.Li h.J.Li J.L.Li J.Q.Li J.S.Li Ke Li L.K.Li Lei Li p.R.Li S.Y.Li w.d.Li w.G.Li X.h.Li X.L.Li Xiaoyu Li z.Y.Li h.Liang h.Liang h.Liang Y.F.Liang Y.T.Liang G.R.Liao L. z. Liao J.Libby C.X.Lin B.J.Liu C.X.Liu d.Liu F.h.Liu Fang Liu Feng Liu h.B.Liu h.M.Liu huanhuan Liu huihui Liu J.B.Liu J.L.Liu J.Y.Liu K.Liu K.Y.Liu L.Liu M.h.Liu p.L.Liu Q.Liu Q.Liu S.B.Liu Shuai Liu T.Liu w.M.Liu X.Liu Y.Liu Y.B.Liu z.A.Liu z.Q.Liu X.C.Lou F.X.Lu h.J.Lu J.d.Lu J.G.Lu X.L.Lu Y.Lu Y.p.Lu C.L.Luo Institute of high Energy physics Beijing 100049China Beihang University Beijing 100191China Beijing Institute of petrochemical Technology Beijing 102617China Bochum Ruhr-University D-44780 BochumGermany Carnegie Mellon University PittsburghPennsylvania 15213USA Central China Normal University Wuhan 430079China China Center of Advanced Science and Technology Beijing 100190China COMSATS University Islamabad Lahore CampusDefence RoadOff Raiwind Road54000 LahorePakistan Fudan University Shanghai 200443China G.I.Budker Institute of Nuclear physics SB RAS(BINp) Novosibirsk 630090Russia GSI helmholtzcentre for heavy Ion Research Gmbh D-64291 DarmstadtGermany Guangxi Normal University Guilin 541004China Guangxi University Nanning 530004China hangzhou Normal University Hangzhou 310036China helmholtz Institute Mainz Staudinger Weg 18D-55099 MainzGermany henan Normal University Xinxiang 453007China henan University of Science and Technology Luoyang 471003China huangshan College Huangshan 245000China hunan Normal University Changsha 410081China hunan University Changsha 410082China Indian Institute of Technology Madras Chennai 600036India Indiana University BloomingtonIndiana 47405USA INFN Laboratori Nazionali di Frascati INFN Laboratori Nazionali di FrascatiI-00044FrascatiItaly INFN Laboratori Nazionali di Frascati INFN Sezione di PerugiaI-06100PerugiaItaly INFN Laboratori Nazionali di Frascati University of PerugiaI-06100PerugiaItaly INFN Sezione di Ferrara INFN Sezione di FerraraI-44122FerraraItaly INFN Sezione di Ferrara University of FerraraI-44122FerraraItaly 25Institute of Modern PhysicsLanzhou 730000China Institute of Modern physics Lanzhou 730000China Institute of physics and Technology Peace Ave.54BUlaanbaatar 13330Mongolia Jilin University Changchun 130012China Johannes Gutenberg University of Mainz Johann-Joachim-Becher-Weg 45D-55099 MainzGermany Joint Institute for Nuclear Research 141980 DubnaMoscow regionRussia Justus-Liebig-Universitaet Giessen II.Physikali

during the 2016-17 and 2018-19 running periods,the BESIII experiment collected 7.5 fb of e^(+)e^(-)collision data at center-of-mass energies ranging from 4.13 to 4.44 *** data samples are primarily used for the study of excited charmonium and charmoniumlike *** analyzing the di-muon process e^(+)e^(-)→(γISR=FSR)μ^(+)μ^(-),we measure the center-of-mass energies of the data samples with a precision of 0.6 *** a run-by-run study,we find that the center-of-mass energies were stable throughout most of the data-collection period.

关键词： center-of-mass energy e^(+)e^(-) annihilation BESIII

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Graphene controlled solid-state growth of oxygen vacancies riched V_(2)O_(5)catalyst to highly activate Fenton-like reaction

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Chinese Chemical Letters 2024年第12期35卷 356-359页

作者： Mengxiang zhu tao ding Yunzhang Li Yuanjie peng Ruiping Liu Quan zou Leilei yang Shenglei Sun pin zhou Guosheng Shi dongting Yue Shanghai Applied Radiation Institute State Key Lab.Advanced Special SteelShanghai UniversityShanghai 200444China Center for Soil protection and land scape design Chinese Academy of Environmental PlanningBeijing 100041China zhejiang Grearth Ecological Co. Ltd.International Trade Center Office BuildingHangzhou 311400China Institute of Balsalt Fiber in Eco-Application School of Environmental Science and EngineeringQingdao UniversityQingdao 266071China Research Center of secondary Resources and Environment Changzhou Institute of TechnologyChangzhou 213032China

Fenton-like process based on metal oxide presents one of the most hoping strategies to generate reactive oxygen species to treat refractory *** introduction of oxygen vacancies(OVs)can enhance the catalytic performance of metal oxides in Fenton-like *** this paper,a one-step all solid-state synthesis strategy is proposed to induce oxygen defects in V_(2)O_(5),which uses graphene to engineer the crystallization process of V-based *** approach employs graphene as a solid-catalyst to promote growth of V-based crystals owing to the ions-πinteractions between graphene and VCl_(3).The electrondonor OVs in V_(2)O_(5)@graphene can not only active h_(2)O_(2)for the·Oh generation,but also accelerate the reduction of V^(5+)and V^(4+),thereby ensuring defective V_(2)O_(5)@graphene/h_(2)O_(2)system is 14.3,28.2,and 17.3times higher than that of graphene/h_(2)O_(2),pure V_(2)O_(5)/h_(2)O_(2)and graphene+V_(2)O_(5)/h_(2)O_(2)(mechanical mixed system),*** study provides a novel synthetic strategy to design and.p.epare OVs-riched transition metal catalysts for developing advanced oxidation technologies toward higher sustainability and.p.acticality.

关键词： Oxygen vacancies Fenton-like Catalysis V_(2)O_(5) Solid-state synthesis

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Concurrent catalytic removal of typical volatile organic compound mixtures over Au-pd/α-MnO2 nanotubes

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Journal of Environmental Sciences 2018年第2期30卷 276-288页

作者： Yunsheng Xia Lu Xia Yuxi Liu tao yang Jiguang deng hongxing dai College of Chemistry and Chemical Engineering Bohai University Jinzhou 121013 China Key Laboratory of Beijing on Regional Air pollution Control Beijing Key Laboratory for Green Catalysis and Separation MOE Laboratory of Enhanced Heat Transfer and Energy Conservation and College of Environmental and Energy Engineering Beijing University of Technology Beijing 100124 China Mechanical Engineering d.p.rtment University of Aveiro 3810-193 Aueiro Portugal

α-MnO2 nanotubes and their supported Au-pd alloy nanocatalysts were prepared using hydrothermal and.p.lyvinyl alcohol-protected reduction methods, respectively. Their catalytic activity for the oxidation of toluene/m-xylene, acetone/ethyl acetate, acetone/m-xylene and ethyl acetate/m-xylene mixtures was evaluated. It was found that the interaction between Au-pd alloy nanoparticles and α-MnO2 nanotubes significantly improved the reactivity of lattice oxygen, and the 0.91 wt.% Au0.48 pd/α-MnO2 nanotube catalyst outperformed the α-MnO2 nanotube catalyst in the oxidation of toluene, m-xylene, ethyl acetate and acetone. Over the0.91 wt.% Au0.48 pd/α-MnO2 nanotube catalyst,（i） toluene oxidation was greatly inhibited in the toluene/m-xylene mixture, while m-xylene oxidation was not influenced;（ii） acetone and ethyl acetate oxidation suffered a minor impact in the acetone/ethyl acetate mixture; and（iii） m-xylene oxidation was enhanced whereas the oxidation of the oxygenated VOCs（volatile organic compounds） was suppressed in the acetone/m-xylene or ethyl acetate/m-xylene mixtures. The competitive adsorption of these typical VOCs on the catalyst surface induced an inhibitive effect on their oxidation, and increasing the temperature favored the oxidation of the VOCs. The mixed VOCs could be completely oxidized into CO2 and h2 O below 320°C at a space velocity of 40,000 m L/（g·hr）. The 0.91 wt.% Au0.48 pd/α-MnO2 nanotube catalyst exhibited high catalytic stability as well as good tolerance to water vapor and CO2 in the oxidation of the VOC mixtures. Thus, the α-MnO2 nanotube-supported noble metal alloy catalysts hold.p.omise for the efficient elimination of VOC mixtures.

关键词： Volatile organic compound mixture Manganese dioxide nanotubes Supported gold.p.lladium alloy catalyst Catalytic oxidation Competitive adsorption

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disruption of MIR396e and MIR396f improves rice yield under nitrogen-deficient conditions

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National Science Review 2020年第1期7卷 102-112页

作者： Jinshan zhang zhenyu zhou Jinjuan Bai Xiaoping tao Ling wang hui zhang Jian-Kang zhu Shanghai Center for plant Stress Biology and Center for Excellence in Molecular plant Sciences Chinese Academy of Sciences University of Chinese Academy of Sciences School of Biotechnology Jiangsu University of Science and Technology Shanghai Engineering Research Center of plant Germplasm Resources College of Life Science Shanghai Normal University d.p.rtment of horticulture and Landscape Architecture Purdue UniversityWest Lafayette

The microRNA miR396 directly represses GROwTh-REGULATING FACTORs(OsGRFs) and has been implicated in regulating rice yield and in nitrogen assimilation. Overexpressing the miR396 targets OsGRF4 and OsGRF6 improves rice yield via increased grain size and.p.nicle branching, respectively. here, we used CRISpR/Cas9 to assess the function of miR396 genes in rice. Knockout of MIR396 ef(MIR396e and MIR396f), but not other isoforms, enhanced both grain size and.p.nicle branching, resulting in increased grain yield. Importantly, under nitrogen-deficient conditions, mir396ef mutants sh.w.d an even higher relative increase in grain yield as well as elevated above-ground biomass. Furthermore, we identified OsGRF8 as a new target of miR396, in addition to the known targets OsGRF4 and OsGRF6. disruption of the miR396-targeting site in OsGRF8 was sufficient to both enlarge grain size and elongate panicles. Our results suggest that rice-seed and.p.nicle development are regulated by miR396ef-GRF4/6/8-GIF1/2/3 modules and that mi R396ef are promising targets of genome editing for breeding environmentally friendly rice varieties that require less nitrogen fertilization.

关键词： microRNA miR396 OsGRF4/6/8 OsGIF1/2/3 nitrogen deficiency grain yield

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Search for electron-antineutrinos associated with gravitational-wave events Gw150914,Gw151012,Gw151226,Gw170104,Gw170608,Gw170814,and Gw170817 at daya Bay

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Chinese physics C 2021年第5期45卷 190-201页

作者： F.p.An A.B.Balantekin h.R.Band M.Bishai S.Blyth G.F.Cao J.Cao J.F.Chang Y.Chang h.S.Chen S.M.Chen Y.Chen Y.X.Chen J.Cheng z.K.Cheng J.J.Cherwinka M.C.Chu J.p.Cummings O.dalager F.S.deng Y.Y.ding M.V.diwan T.dohnal J.dove M.dvorak d.A.dwyer J.p.Gallo M.Gonchar G.h.Gong h.Gong w.Q.Gu J.Y.Guo L.Guo X.h.Guo Y.h.Guo z.Guo R.w.hackenburg S.hans M.he K.M.heeger Y.K.heng A.higuera Y.K.hor Y.B.hsiung B.z.h. J.R.hu T.hu z.J.hu h.X.huang X.T.huang Y.B.huang p.huber d.E.Jaffe K.L.Jen X.L.Ji X.p.Ji R.A.Johnson d.Jones L.Kang S.h.Kettell S.Kohn M.Kramer T.J.Langford J.Lee J.h.C.Lee R.T.Lei R.Leitner J.K.C.Leung F.Li J.J.Li Q.J.Li S.Li S.C.Li w.d.Li X.N.Li X.Q.Li Y.F.Li z.B.Li h.Liang C.J.Lin G.L.Lin S.Lin J.J.Ling J.M.Link L.Littenberg B.R.Littlejohn J.C.Liu J.L.Liu C.Lu h.Q.Lu J.S.Lu K.B.Luk X.B.Ma X.Y.Ma Y.Q.Ma C.Marshall d.A.Martinez Caicedo K.T.Medonald R.d.McKeown Y.Meng J.Napolitano d.Naumov E.Naumova J.p.Ochoa-Ricoux A.OIshevskiy h.-R.pan J.park S.patton J.C.peng C.S.J.pun F.z.Qi M.Qi X.Qian N.Raper J.Ren C.Morales Reveco R.Rosero B.Roskovec X.C.Ruan h.Steiner J.L.Sun T.Tmej K.Treskov w.-h.Tse C.E.Tull B.Viren V.Vorobel C.h.w.ng J.wang M.wang N.Y.wang R.G.wang w.wang w.wang X.wang Y.wang Y.F.wang z.wang z.wang z.M.wang h.Y.wei L.h.w.i L.J.wen K.whisnant C.G.white h.L.h.w.ng E.worcester d.R.wu F.L.wu Q.wu w.J.wu d.M.Xia z.Q.Xie z.z.Xing J.L.Xu T.Xu T.Xue C.G.yang L.yang Y.z.yang h.F.Yao M.Ye M.Yeh B.L.Young h.z.Yu z.Y.Yu B.B.Yue S.zeng Y.zeng L.zhan C.zhang F.Y.zhang h.h.zhang J.w.zhang Q.M.zhang X.T.zhang Y.M.zhang Y.X.zhang Y.Y.zhang z.J.zhang z.p.zhang z.Y.zhang J.zhao L.zhou h.L.zhuang J.h.zou Instiute of Modern physics East China University of Science and TechnologyShanghai 200237 University of wisconsin MadisonWisconsin 53706 wright Laboratory and d.p.rtment of physics Yale UniversityNew HavenConnecticut 06520 Brookhaven National Laboratory UptonNew York 11973 d.p.rtment of physics National Taiwan UniversityTaipei Institute of high Energy physics Beijing 100049 National United University Miao-Li d.p.rtment of Engineering physics Tsinghua UniversityBejing 100084 Shenzhen University Shenzhen 518060 Sun Yat-Sen(zhongshan)University Guangzhou North China Electric power University Bejjing 102206 Chinese University of hong Kong Hong Kong 999077 Siena College LoudonvilleNew York 12211 d.p.rtment of physics and Astronomy University of CaliforniaIrvineCaliformia 92697 University of Science and Technology of China Hefei 230026 Charles University Faculty of Mathematics and PhysicsPrague d.p.rtment of physics University of llinois at Urbana-ChampaignUrbanaIlinois 61801 Lawrence Berkeley National Laboratory BerkeleyCalifornia 94720 d.p.rtment of physics Illinois Institute of TechnologyChicagoIllinois 60616 Joint Institute for Nuclear Research DubnaMoscow Region Beijing Normal University Beijing 100875 d.p.rtment of Nuclear Science and Technology School of Energy and Power EngineeringXi'an Jiaotong UniversityXi'an 710049 d.p.rtment of physics University of HoustonHoustonTexas 77204 China Institute of Atomic Energy Beijing 102413 Shandong University Jinan 250100 Guangxi University Nanning 530004 Center for Neutrino physics Virginia TechBlacksburgVirginia 24061 Institute of physics National Chiao-Tung UniversityHsinchu d.p.rtment of physics University of CincinnatiCincinnatiOhio 45221 d.p.rtment of physies Collge of Science and TechnologyTemple UniversityPhiladelphiaPennsylvania 19122 dongguan University of Technology Dongguan 523808 d.p.rtment of physics University of CaliforniaBerkeleyCalifornia 94720 d.p.rtment of physics The University of Hong KongPokfulamHong Kong 999077 School

The establishment of a possible connection between neutrino emission and gravitational-wave(Gw)bursts is important to our understanding of the physical processes that occur when black holes or neutron stars *** the daya Bay experiment,using the data collected from december 2011 to August 2017,a search.w.s per-formed for electron-antineutrino signals that coincided with detected Gw events,including Gw150914,Gw151012,Gw151226,Gw170104,Gw170608,Gw 170814,and Gw *** used three time windows of±10,±500,and±1000 s relative to the occurrence of the Gw events and a neutrino energy range of 1.8 to 100 MeV to search for correlated neutrino *** detected electron-antineutrino candidates were consistent with the expected background rates for all the three time *** monochromatic spectra,we found upper limits(90%confidence level)of the electron-antineutrino fluence of(1.13-2.44)×10^(11)cm^(-2)at 5 MeV to 8.0×10^(7)cm^(-2)at 100 MeV for the three time w *** the assumption of a Fermi-dirac spectrum,the upper limits were found to be(5.4-7.0)×10^(9)cm^(2)for the three time windows.

关键词： grav itational waves electron-antineutrinos fluence upper limit

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Analysis of human urine metabolites using SpE and NMR spectroscopy

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Science China Chemistry 2008年第3期51卷 218-225页

作者： yang weiJin wANG Yawei zhou QunFang TANG huiRu State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China State Key Laboratory of Magnetic Resonance and Atomic and Molecular physics Wuhan Centre for Magnetic ResonanceWuhan Institute of Physics and MathematicsChinese Academy of SciencesWuhan 430071China

Nuclear magnetic resonance (NMR) spectroscopic analysis of metabonome/metabolome has widespread applications in biomedical science researches. h.w.ver, most of NMR resonances for urinary metabolites remain to be fully assigned. In the present study, human urine samples from two healthy volunteers were pre-treated with C18 solid.p.ase extraction and the resultant 5 sub-fractions were subjected to one- and two-dimensional NMR studies, including 1h J-Resolved, 1h-1h COSY, 1h-1h TOCSY, 1h-13C hSQC, and hMBC 2d NMR. More than 70 low molecular weight metabolites were identified, and complete assignments of 1h and 13C resonances including many complex coupled spin systems were obtained.

关键词： urine metabolites NMR spectroscopy solid phase extraction metabonomics/metabolomics

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Reduced Smoothened level rescues Aβ-induced memory deficits and neuronal inflammation in animal models of Alzheimer's disease

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Journal of Genetics and Genomics 2018年第5期45卷 237-246页

作者： weiwei Ma Mengnan wu Siyan zhou Ye tao zuolei Xie Yi zhong School of Life Sciences Tsinghua UniversityBeijing 100084China Suzhou Joekai Biotechnology LLC Suzhou 215347 China Beijing Joekai Biotechnology LLC Beijing 100094China

Emerging evidence suggests that neuro-inflammation begins early and drives the pathogenesis of Alzheimer＇s disease （Ad）, and anti-inflammatory therapies are under clinical development. h.w.ver, several anti-inflammatory compounds failed to improve memory in clinical trials, indicating that reducing inflammation alone might not be enough. On the other hand, neuro-inflammation is implicated in a number of mental disorders which share the same therapeutic targets. Based on these observations, we screened a batch of genes related with mental disorder and neuro-inflammation in a classical olfactory conditioning in an amyloid beta （Aβ） overexpression fly model. A Smoothened （SMO） mutant was identified as a genetic modifier of Aβ toxicity in 3-min memory and downregulation of SMO rescued Aβ- induced 3-min and 1-h memory deficiency. Also, Aβ activated innate inflammatory response in fly by increasing the expression of antimicrobial peptides, which.w.re alleviated by downregulating SMO. Furthermore, pharmaceutical administration of a SMO antagonist LdE rescued Aβ-induced upregulation of SMO in astrocytes of mouse hippocampus, improved memory in Morris water maze （MwM）, and reduced expression ofastrocyte secreting pro-inflammatory factors IL-1β, TNFα and the microglia marker IBA-1 in an App/pS1 transgenic mouse model. Our study suggests that SMO is an important conserved modulator of Aβ toxicity in both flv and mouse models of Ad.

关键词： Alzheimer disease Inflammation Learning and memory Smoothened

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An efficient protocol of cryo-correlative light and electron microscopy for the study of neuronal synapses

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Biophysics Reports 2019年第3期5卷 111-122页

作者： Rong Sun Yun-tao Liu Chang-Lu tao Lei Qi pak-Ming Lau z.h.ng zhou Guo-Qiang Bi Center for Integrative Imaging Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences University of Science and Technology of China Chinese Academy of Sciences Key Laboratory of Brain Function and disease University of Science and Technology of China The California Nano Systems Institute and d.p.rtment of Microbiology Immunology and Molecular GeneticsUniversity of California Chinese Academy of Sciences Center for Excellence in Brain Science and Intelligence Technology University of Science and Technology of China

Cryo-electron tomography is an emerging electron microscopy technique for determining three-dimensional structures of cellular architectures near their native state at nanometer resolution, with a shortcoming of lack of specific labels. Fluorescence light microscopy, on the other hand, specifically visualizes target cellular and molecular components with fluorescent labels, but is limited to a resolution of tens to hundreds nanometers. Combining the advantages of the two techniques, we have developed a cryocorrelative light and electron microscopy system. Our system consists a custom-designed cryo-chamber that allows for fluorescence imaging of frozen-hydrated samples, and an algorithm to achieve accurate correlation. with this system and our optimized.p.otocol, high-quality tomograms of neuronal synapses labelled by specific fluorescent tags in cultured hippocampal neurons are obtained at high efficiency.

关键词： Cryo-correlative light and electron microscopy Cryo-electron tomography Neuronal synapse

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