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A facile strategy for tuning the density of surface-grafted biomolecules for melt extrusion-based additive manufacturing applications

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Bio-Design and Manufacturing 2024年第3期7卷 277-291页

作者： I.A.O.Beeren G.Dos Santos P.J.Dijkstra C.Mota J.Bauer h.ferreira Rui L.Reis N.Neves S.Camarero-Espinosa M.B.Baker L.Moroni Department of Complex Tissue Regeneration MERLN Institute for Technology-Inspired Regenerative MedicineMaastricht University6229 ER MaastrichtThe Netherlands 3B’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3B’s-PT Government Associate Laboratory 4806-909 Braga/GuimarãesPortugal POLYMAT University of the Basque Country UPV/EHU20018 Donostia/San SebastiánSpain IKERBASQUE Basque Foundation for Science48009 BilbaoSpain

Melt extrusion-based additive manufacturing(ME-AM)is a promising technique to fabricate porous scaffolds for tissue engi-neering ***,most synthetic semicrystalline polymers do not possess the intrinsic biological activity required to control cell *** of biomolecules on polymeric surfaces of AM scaffolds enhances the bioactivity of a construct;however,there are limited strategies available to control the surface ***,we report a strategy to tune the surface density of bioactive groups by blending a low molecular weight poly(ε-caprolactone)5k(PCL5k)containing orthogonally reactive azide groups with an unfunctionalized high molecular weight PCL75k at different *** porous three-dimensional(3D)scaf-folds were then fabricated using a high weight percentage(75 wt.%)of the low molecular weight PCL *** a proof-of-concept test,we prepared films of three different mass ratios of low and high molecular weight polymers with a thermopress and reacted with an alkynated fluorescent model compound on the surface,yielding a density of 201-561 pmol/cm^(2).Subsequently,a bone morphogenetic protein 2(BMP-2)-derived peptide was grafted onto the films comprising different blend compositions,and the effect of peptide surface density on the osteogenic differentiation of human mesenchymal stromal cells(hMSCs)was *** two weeks of culturing in a basic medium,cells expressed higher levels of BMP receptor II(BMPRII)on films with the conjugated *** addition,we found that alkaline phosphatase activity was only significantly enhanced on films contain-ing the highest peptide density(i.e.,561 pmol/cm^(2)),indicating the importance of the surface *** together,these results emphasize that the density of surface peptides on cell differentiation must be considered at the cell-material ***,we have presented a viable strategy for ME-AM community that desires to tune the bulk and surface functionality via blending of(m

关键词： Additive manufacturing Blending Surface functionalization Surface density Click chemistry human

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Targeting PCSK9:a promising adjuvant strategy in cancer immunotherapy

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Signal Transduction and Targeted Therapy 2021年第4期6卷 1081-1083页

作者： Catarina R.Almeida Beatriz h.ferreira lola F.Duarte iBiMED-Institute of Biomedicine Department of Medical SciencesUniversity of AveiroAveiroPortugal CICECO-Aveiro Institute of Materials Department of ChemistryUniversity of AveiroAveiroPortugal

Treatment with immune checkpoint inhibitors(ICIs)has drastically improved the prognosis of certain advanced-stage ***,low response rates and the development of resistance remain important *** a study recently published in Nature,Li et *** that antibody inhibitors of proprotein convertase subtilisin/kexin type 9(PCSK9),FDA-approved for hyperlipidemia treatment,have the potential to boost the anticancer efficacy of ICIs.

关键词： al. prognosis adjuvant

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A Rapid Parameter of Enzyme-Treated Cellulosic Material Revealed by Reducing Sugar Release

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Journal of Renewable Materials 2024年第3期12卷 539-551页

作者： Verônica Távilla ferreira Silva Adriane Maria ferreira Milagres Departamento de Biotecnologia Escola de Engenharia de LorenaUniversidade de São PauloLorenaSP12602-810Brazil

This study was conducted to evaluate the effectiveness of enzymes in purifying and reducing the degree of polymerization of cellulose for the production of dissolving *** goal was to determine the contributions of xylanase(X)and endoglucanase(EG)in the treatment of pulp,specifically by quantifying the formation of soluble and insoluble reducing sugars using the dinitrosalycilic acid(DNS)***,the release of soluble reducing sugars(RSSol)was enhanced after xylanase treatment,while endoglucanase(EG)treatment led to changes in insoluble reducing sugars(RSIns).The maximum synergism was observed for RSIns when a high ratio of endoglucanase to xylanase(320EG:5X/g pulp)was *** relative contribution of endoglucanase to RSins was determined to be 15.6%of the total reducing *** viscosity of pulps treated with xylanase decreased only by 7%,whereas endoglucanase treatment significantly reduced viscosity by 45%.Modifications in the particle size were observed after pulp treatment with the combination of endoglucanase and *** summary,the DNS test is a rapid and effective method for evaluating the efficiency of enzyme treatments on *** measurement of RSIns correlates with changes in pulp viscosity to different extents,providing valuable insights into the effectiveness of enzyme treatments.

关键词： Endoglucanase xylanase cellulose fiber pulp synergism

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Reducing strain on primary healthcare systems through innovative models of care:the impact of direct access physiotherapy for musculoskeletal conditions—an interrupted time series analysis

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Family Medicine and Community health 2024年第3期12卷 1-12页

作者： James henry Zouch Bjørnar Berg Are hugo Pripp Kjersti Storheim Claire E Ashton-James Manuela L ferreira Margreth Grotle Paulo h.ferreira Sydney Musculoskeletal health Faculty of Medicine and HealthThe University of SydneySydneyNew South WalesAustralia Centre for Intelligent Musculoskeletal health Oslo Metropolitan University Faculty of Health SciencesOsloNorway Oslo Metropolitan University Faculty of health Sciences OsloNorway Oslo Centre of Biostatistics and Epidemiology Oslo University HospitalOsloNorway Department of Rehabilitation Science and health Technology Oslo Metropolitan University Faculty of Health SciencesOsloNorway Department of Research and Innovation Oslo University Hospital Division of Clinical NeuroscienceOsloNorway Sydney Medical School Kolling InstituteThe University of Sydney Faculty of Medicine and HealthSydneyNew South WalesAustralia The George Institute for Global health SydneyNew South WalesAustralia Department of Research and Innovation Oslo University HospitalOsloNorway Department of Rehabilitation and Technology Oslo Metropolitan University Faculty of Health SciencesOsloNorway Charles Perkins Centre Sydney Musculoskeletal HealthThe University of Sydney Faculty of Medicine and HealthSydneyNew South WalesAustralia

Objectives To evaluate the longitudinal impact of introducing a national,direct access physiotherapy model of care on the rates of primary and secondary care consultations for musculoskeletal(MSK)*** Interrupted time series analysis using segmented linear *** Norway primary care Participants A cohort of 82072 participants was derived from 3 population-based health surveys conducted across separate geographical regions in *** participants surveyed were eligible for inclusion as a national representative sample of the Norwegian *** MSK consultations were linked to the Norwegian Control and Payment of health Reimbursement database and the Norwegian Patient Register using the International Classification of Primary Care diagnostic medical codes L-chapter for MSK conditions and spine related International Classification of Diseases,10th Revision,*** Direct access to physiotherapy model of care introduced nationally in Norway in *** model allowed Norwegians to consult directly with qualified physiotherapists for MSK conditions(eg,back pain,knee osteoarthritis)without the need for a medical referral in order to claim a social security *** outcomes measured Rates of primary care consultations per 10000 population(general practitioner(GP)and physiotherapist consultations)and secondary care(specialist consultations and surgical procedures)measured from 2014 to *** The introduction of the direct access physiotherapy model was associated with an immediate stepped reduction of 391 general practice consultations per 10000 population,(95%CI:−564 to−216),without an associated change in physiotherapy *** analyses suggested there was an associated reduction in physiotherapy consultations for those in the lowest education group of 150 consultations per quarter(95%CI:−203 to−97),70 consultations per quarter in the intermediate education group(95%CI:−115 to−27)and a step

关键词： impact analysis strain

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Evaluation of the efficiency of urban solid waste management in Brazil by data envelopment analysis and possible variables of influence

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Waste Disposal and Sustainable Energy 2024年第2期6卷 283-295页

作者： Isabel das Merces Costa Marta ferreira Dias Margarita Robaina GOvCOPP-Research Unit on Governance Competitiveness and Public Policies and Analyst of UniversidadeEstadual de Feira de SantanaUniversidade de AveiroAveiroPortugal GOvCOPP-Research Unit on Governance Competitiveness and Public PoliciesDEGEIT-Department of EconomicsManagementIndustrial Engineering and TourismUniversidade de AveiroAveiroPortugal

This article has the general objective of estimating the efficiency of urban solid waste management in 940 Brazilian munici-palities through Data Envelopment Analysis(DEA)technique and has specific objectives:(i)to estimate efficiency scores;(ii)to compare the performance between different groups of municipalities;and(ii)to analyze the profile of efficient munici-palities from the perspective of the guidelines of Law 12,305/2010 and socio-economic and environmental *** technique used was DEA with output-oriented and variable scale to return *** results showed higher efficiency scores in the municipalities with populations above 500,000 *** score variation ranged from 0.5(municipalities with populationshabitants)to 0.9(municipalities with more than 500,000 inhabitants).Of the sample set,only 12.34%of the municipalities were considered efficient,and when analyzing the efficient group,it was found that adherence to legislation was not a major factor in achieving efficiency.

关键词： Urban solid waste management Data envelopment analysis Efficiency Brazil Municipalities

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Dental-derived stem cells in tissue engineering:the role of biomaterials and host response

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Regenerative Biomaterials 2024年第1期3卷 17-26页

作者： Weihao Yuan Luiza de Almeida Queiroz ferreira Bo Yu Sahar Ansari Alireza Moshaverinia Weintraub Center for Reconstructive Biotechnology Section of ProsthodonticsSchool of DentistryUniversity of CaliforniaLos AngelesLos AngelesCA 90095USA Department of Restorative Dentistry School of DentistryUniversidade Federal de Minas GeraisBelo HorizonteMinas GeraisBrazil Section of Restorative Dentistry School of DentistryUniversity of CaliforniaLos AngelesLos AngelesCA 90095USA Department of Bioengineering Henry Samueli School of Engineering and Applied SciencesUniversity of CaliforniaLos AngelesLos AngelesCA 90095USA

Dental-derived stem cells(DSCs)are attractive cell sources due to their easy access,superior growth capacity and low *** can respond to multiple extracellular matrix signals,which provide biophysical and biochemical cues to regulate the fate of residing ***,the direct transplantation of DSCs suffers from poor proliferation and differentiation toward functional cells and low survival rates due to local ***,elegant advances in the design of novel biomaterials have been made to give promise to the use of biomimetic biomaterials to regulate various cell behaviors,including proliferation,differentiation and *** could be tailored with multiple functionalities,e.g.,*** is an emerging need to summarize recent advances in engineered biomaterials-mediated delivery and therapy of DSCs and their potential ***,we outlined the design of biomaterials for supporting DSCs and the host response to the transplantation.

关键词： dental-derived stem cells biomaterials host response tissue engineering

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Contribution of glial cells to the neuroprotective effects triggered by repetitive magnetic stimulation:a systematic review

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Neural Regeneration Research 2024年第1期19卷 116-123页

作者： Susana A.ferreira Nuno Pinto Inês Serrenho Maria Vaz Pato Graça Baltazar Centro de Investigação em Ciências da Saúde(CICS-UBI) Universidade da Beira InteriorCovilhãPortugal Faculdade de Ciências da Saúde Universidade da Beira InteriorCovilhãPortugal GRUBI-Systematic Reviews Group University of Beira InteriorCovilhãPortugal

Repetitive transcranial magnetic stimulation has been increasingly studied in different neurological diseases,and although most studies focus on its effects on neuronal cells,the contribution of nonneuronal cells to the improvement trigge red by repetitive transcranial magnetic stimulation in these diseases has been increasingly *** systematically review the effects of repetitive magnetic stimulation on non-neuronal cells two online *** of Science and PubMed were searched fo r the effects of high-frequency-repetitive transcranial magnetic stimulation,low-frequencyrepetitive transcranial magnetic stimulation,intermittent theta-bu rst stimulation,continuous thetaburst stimulation,or repetitive magnetic stimulation on non-neuronal cells in models of disease and in unlesioned animals or cells.A total of 52 studies were *** protocol more frequently used was high-frequency-repetitive magnetic stimulation,and in models of disease,most studies report that high-frequency-repetitive magnetic stimulation led to a decrease in astrocyte and mic roglial reactivity,a decrease in the release of pro-inflammatory cyto kines,and an increase of oligodendrocyte *** trend towards decreased microglial and astrocyte reactivity as well as increased oligodendrocyte proliferation occurred with intermittent theta-burst stimulation and continuous theta-burst *** papers analyzed the low-frequency-repetitive transcranial magnetic stimulation protocol,and the parameters evaluated were restricted to the study of astrocyte reactivity and release of pro-inflammatory cytokines,repo rting the absence of effects on these paramete *** what concerns the use of magnetic stimulation in unlesioned animals or cells,most articles on all four types of stimulation reported a lack of *** is also important to point out that the studies were developed mostly in male rodents,not evaluating possible diffe rential effects of repetitive transcranial magneti

关键词： astrocyte glia high-frequency repetitive magnetic stimulation inflammation low-frequency repetitive magnetic stimulation microglia neurologic disorders oligodendrocyte repetitive magnetic stimulation theta-burst stimulation

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Bias-Free Solar-to-hydrogen Conversion in a BiVO4/PM6:Y6 Compact Tandem with Optically Balanced Light Absorption

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Energy & Environmental Materials 2024年第4期7卷 231-238页

作者： Catarina G.ferreira Constanza Sansierra Francisco Bernal-Texca Mingyu Zhang Carles Ros Jordi Martorell ICFO–Institut de Ciències Fotòniques The Barcelona Institute of Science and Technology Departament de Física Universitat Politècnica de Catalunya

The high voltage required to overcome the thermodynamic threshold and the complicated kinetics of the water splitting reaction limit the efficiency of single semiconductor-based photoelectrochemistry.A semiconductor/solar cell tandem structure has been theoretically demonstrated as a viable path to achieve an efficient direct transformation of sunlight into chemical energy.

关键词： bismuth vanadate compact tandem organic solar cells photonic multilayers solar-to-hydrogen conversion

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Temporal and spatial differences in human activities performed in Urban Green Spaces of Vilnius(Lithuania)

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Geography and Sustainability 2024年第2期5卷 302-317页

作者： Luís Valença Pinto Carla Sofia Santos ferreira Paulo Pereira Research Centre for Natural Resources Environment and Society(CERNAS) Polytechnic Institute of CoimbraBencanta3045-601 CoimbraPortugal Environmental Management Laboratory Mykolas Romeris UniversityVilniusLithuania Polytechnic Institute of Coimbra Applied Research InstituteRua da MisericórdiaLagar dos Cortiços-S.Martinho do Bispo3045-093 CoimbraPortugal

Urban green spaces(UGS)are relevant to city well-being,as recognized by the United Nations’Sustainable Development Goals(SDGs).however,few studies have studied the temporal use of *** work assessed the seasonal,weekly,and daily use of three urban green spaces(Vingis Park,Bernardino Garden,and Jomantas Park)in Vilnius(Lithuania).The study is based on an on-site observation-based survey,which recorded users’characteristics,activities,and weather conditions during summer and *** results showed that UGS’s seasonal,weekly,and daily use differed according to park and users’*** with a higher diversity of facilities had a high seasonal difference in the number of observed *** numbers were higher in the summer for activities with children,social activities,sports,and water activities than in the *** Park had the lowest variability in user *** variables were linked to changes in users’*** precipitation and lower temperature were associated with reducing the number of users and the diversity of registered *** of the stationary activities were observed during *** diversity of the observed activities was associated with the available facilities rather than the park *** distribution of stationary activities was spatially correlated with facility/equipment(benches,playgrounds,sports,and fitness equipment)and proximity to water *** results of this study are relevant for UGS design,planning,and management.

关键词： Urban parks Seasonal differences Temporal use Time of day Spatial distribution of UGS users

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RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation

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World Journal of Stem Cells 2024年第6期16卷 656-669页

作者： Daniel B ferreira Leticia M Gasparoni Cristiane F Bronzeri Katiucia B S Paiva Laboratory of Extracellular Matrix Biology and Cellular Interaction Department of AnatomyInstitute of Biomedical SciencesUniversity of São PauloSão Paulo 05508-000São PauloBrazil

BACKGROUND Validation of the reference gene(RG)stability during experimental analyses is essential for correct quantitative real-time polymerase chain reaction(RT-qPCR)data ***,in an unreliable way,several studies use genes involved in essential cellular functions[glyceraldehyde-3-phosphate dehydro-genase(GAPDh),18S rRNA,andβ-actin]without paying attention to whether they are suitable for such experimental conditions or the reason for choosing such ***,such studies use only one gene when Minimum Information for Publication of Quantitative Real-Time PCR Experiments guidelines recom-mend two or more *** impacts the credibility of these studies and causes dis-tortions in the gene expression *** tissue engineering,the accuracy of gene expression drives the best experimental or therapeutical *** cultivated DPSCs under two conditions:Undifferentiated and osteogenic dif-ferentiation,both for 35 *** evaluated the gene expression of 10 candidates for RGs[ribosomal protein,large,P0(RPLP0),TATA-binding protein(TBP),GAPDh,actin beta(ACTB),tubulin(TUB),aminolevulinic acid synthase 1(ALAS1),tyro-sine 3-monooxygenase/tryptophan 5-monooxygenase activation protein,zeta(YWhAZ),eukaryotic translational elongation factor 1 alpha(EF1a),succinate dehydrogenase complex,subunit A,flavoprotein(SDhA),and beta-2-micro-globulin(B2M)]every 7 d(1,7,14,21,28,and 35 d)by *** data were analysed by the four main algorithms,ΔCt method,geNorm,NormFinder,and BestKeeper and ranked by the RefFinder *** subdivided the samples into eight *** All of the data sets from clonogenic and osteogenic samples were analysed using the RefFinder *** final ranking showed RPLP0/TBP as the two most stable RGs and TUB/B2M as the two least stable *** theΔCt method or NormFinder analysis showed TBP/RPLP0 as the two most stable ***,geNorm analysis showed RPLP0/EF1αin the first *** algorithms’two least stab

关键词： Dental pulp stem cells Reference gene housekeeping gene Endogenous gene Osteogenic differentiation RefFinder

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