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Activity of T Cells Stimulated by hemagglutinin-neuraminidase of Newcastle Disease Virus in vivo

Chemical Research in Chinese Universities 2011年第3期27卷 455-460页

作者： PIAO Bing-guo LI xiao sun Li-li KAN Shi-fu LIU Lei hUANG hai-yan YANG Guo-hua WANG Yu-hang WANG Zhuo-yue sun Jiu-hua PIAO Yun-feng JIN Ning-yi The First hospital of Jilin University Changehun 130021 P. R. ChinA Genetic Engineering Laboratory of PLA 3. Key Laboratory of Jilin Province for Zoonosis Prevention and Control Military Veterinary Institute Academy of Military Medical Sciences of PLA Changehun 130062 P. R. China Key Laboratory of Jilin Province for Zoonosis Prevention and Control Military Veterinary Institute Academy of Military Medical Sciences of PLA Changchun 130062 P. R. China Department of Gastroenterology 209 Hospital of PLA Mudanjiang 157000 P. R. China head and Neck Surgery Tumor Hospital of Jilin Province Changchun 130001 P R. China

To investigate the stimulated activity of T cells and the anti-tumor properties of hemagglutinin-neuraminidase（hN） of Newcastle disease virus（NDV） strain Changchun（NDVcc）, the expression of hN gene in hepatoma cells（human hepG-2 and mouse h22 cells） infected with the recombinant adenovirus（Ad-hN） was identified by Western blot analysis and flow cytometry. Sialidase activity of NDVcc hN expressed by Ad-hN was assayed by the periodate-resorcinol method. The in vivo anti-tumor effects of NDVcc hN were evaluated in the h22 solid tumor model. Regional lymph nodes of the mouse model treated with Ad-hN were removed to harvest T lymphocytes and evaluating the specific cytotoxicity of cytotoxic T lymphocyte（CTL） and natural killer（NK） cells by an L-lactate dehydrogenase（LDh） assay, in the mean time, the secretion of cytokines was analyzed by enzyme linked immunosorbent assays（ELISA）. The results show that NDVcc hN was effectively expressed by Ad-hN in hepG-2 and h22 cells. The sialidase activity assay showed that Ad-hN significantly reduced sialic acid level of the hepatoma cells compared with the cells infected the empty adenovirus vector（Ad-mock）. When treated with Ad-hN, the growth of subcutaneous h22 primary tumors in C57BL/6 mice was suppressed, and the mean mice survival increased. In addition, the treatment of Ad-hN elicited strong NK and CTL responses, and high levels of Th1 cytokines, such as IL-2 and IFN-γ. In conclusion, NDVcc hN effectively elicits T cell-mediate anti-tumor cytotoxicity via sialidase activity and may be a novel strategy for cancer immunotherapy.

关键词： Newcastle disease virus hemagglutinin-neuraminidase hepatoma T Cell Anti-tumor immunity

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Nonlinear relationship between enteric-coated mycophenolate sodium dose and mycophenolic acid exposure in han kidney transplantation recipients

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Acta Pharmaceutica Sinica B 2017年第3期7卷 347-352页

作者： Jun Zhang Mengmeng Jia Lihua Zuo Na Li Yonggang Luo Zhi sun xiaojian Zhang Zhenfeng Zhu Department of Pharmacy The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Department of Integrated Intensive Care Unit The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China

The aim of the research was to investigate the pharmacokinetics(PK) of enteric-coated mycophenolate sodium(EC-MPS) by quantification of the active metabolite of mycophenolic acid(MPA)after multiple escalating oral doses in han kidney transplant recipients. A total of 28 han postoperative kidney transplant recipients were given a multiple-dose of 540, 720 or 900 mg of EC-MPS two times a day in combination with tacrolimus for 6 days. Blood specimens were collected at each time point from0 to 12 h after EC-MPS administration. MPA plasma concentrations were measured by UPLC–UV. The relationship between the EC-MPS dose and its PK parameters was assessed. In the range from 540 to900 mg, C_(max) and AUC_(0–12h) did not increase with dose escalation. The AUC_(0–12h), C_(max), C_0 and T_(max) for the 540 720 and 900 mg doses were not significantly different, respectively(P 40.05). AUC_0–12 h and C_(max) were increased less than proportionally with increasing EC-MPS dose levels. Inter-individual variability in AUC_(0–12h), C_(max) and C_0 were considerable. Nonlinear PK relationships were found from the doses of 540–900 mg of EC-MPS.

关键词： Enteric-coated Mycophenolate sodium Mycophenolic acid Nonlinear dynamics Kidney transplantation Pharmacokinetics UPLC-UV human plasma

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Preparation Methods and Properties of PdCu Alloy Membrane for hydrogen Transition

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贵金属 2012年第A1期33卷 218-221页

作者： sun xiaoliang MA Guang LI Jin ZhANG Ke Northwest Institute for Nonferrous Metal Research Xi'an710016China

Pd and Pd alloy membranes are of increasing interest for hydrogen separation and purification due to their good thermal stability, high permeability and perfect selectivity. PdCu alloy (60wt% Pd) membranes have similar hydrogen permeability compared with PdAg alloy; meanwhile, it is cheaper than PdAg alloy. Furthermore, it has been reported that PdCu membrane has better resistance to poisoning and deactivation by h 2 S impurity. This paper reviews the properties and manufacturing methods of PdCu alloy membrane, finally, introduced some achievement made by us on PdCu alloy membrane.

关键词： PdAg PdCu alloy membrane hydrogen permeation poisoning

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Incorporation of N-doped biochar into zero-valent iron for efficient reductive degradation of neonicotinoids:mechanism and performance

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Biochar 2023年第1期5卷 1343-1359页

作者： xiangying Li xiangyu Zhang Peng Zhang xinhua Wang hongwen sun Yongyue Lu Le Jiao Chenglan Liu National Key Laboratory of Green Pesticide South China Agricultural University483#Wushan RoadTianhe DistrictGuangzhou 510642China MOE Key Laboratory of Pollution Processes and Environmental Criteria College of Environmental Science and EngineeringNankai University38#Tongyan RoadHaihe Education ParkJinnan DistrictTianjin 300350China Agro-Environmental Protection Institute Ministry of Agriculture and Rural Affairs31#Fukang RoadNankai DistrictTianjin 300191China

The extensive use of neonicotinoids on food crops for pest management has resulted in substantial environmental *** is imperative to develop an effective remediation material and technique as well as to determine the evolution pathways of ***,novel ball-milled nitrogen-doped biochar(NBC)-modified zero-valent iron(ZVI)composites(named MNBC-ZVI)were fabricated and applied to degrading *** on the characterization results,NBC incorporation introduced N-doped sites and new allying heterojunctions and achieved surface charge redistribution,rapid electron transfer,and higher hydrophobicity of ZVI *** a result,the interaction between ZVI particles and thiamethoxam(a typical neonicotinoid)was improved,and the adsorption-desorption and reductive degradation of thiamethoxam and·h generation steps were ***-ZVI could rapidly degrade 100%of 10 mg·L^(−1) thiamethoxam within 360 min,its reduction rate constant was 12.1-fold greater than that of pristine ZVI,and the electron efficiency increased from 29.7%to 57.8%.This improved reactivity and selectivity resulted from increased electron transfer,enhanced hydrophobicity,and reduced accumulation of iron ***,the degradation of neonicotinoids occurred mainly via nitrate reduction and dichlorination,and toxicity tests with degradation intermediates revealed that neonicotinoids undergo rapid ***,MNBCZVI also presented favorable tolerance to various anions,humic acid,wastewater and contaminated soil,as well as high *** work offers an efficient and economic biochar-ZVI remediation technology for the rapid degradation and detoxification of neonicotinoids,significantly contributes to knowledge on the relevant removal mechanism and further advances the synthesis of highly reactive and environmentally friendly materials.

关键词： Zero-valent iron(ZVI) Ball milling N-doped biochar Neonicotinoid degradation Electron efficiency

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Synthesis and Structure of A Novel Quasi-Two Dimensional Organic-inorganic hybrid Coordination Polymer:{[Cu(en)_2][Ag_2I_4]}_n

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Chinese Journal of Structural Chemistry 2006年第2期25卷 149-152页

作者： sun Ling-Guo LI hao-hong ChEN Zhi-Rong hUANG Chang-Cang ZhAO Bin LI Jun-Qian College of Chemistry and Chemical Engineering Fuzhou University Fuzhou Fujian 350002 China

A novel coordination polymer {[Cu（en）2][Ag2I4]}, （en = ethylenediamine） was synthesized by the reaction of NaAgI2 and Cu（en）2（NO3）2·2h2O at room temperature with ph = 6.0, and the structure was characterized by x-ray single-crystal diffraction. It crystallizes in monoclinic, space group C2/m, with a=10.646（2）, b = 13.304（3）, c = 6.8445（14） ,A°, β = 118.95（3）°, C4h16N4CuAg2I4, Mr = 907.10, V= 848.3（4） A°^3, Z= 2, Dc = 3.551 g/cm^3, F（000） = 806, μ（MoKa） = 10.787 mm^-1, the final R1 = 0.0256 and wR2 = 0.0654 for 900 observed reflections with I 〉 2σ（I）. According to structural analysis, the title compound consists of template cation [Cu（en）2]^2＋ and inorganic chain [Ag2I4]n^2-. The polymeric negative chain [Ag2I4]n^2- is built up from pairs of AgI4 tetrahedron by sharing one edge. Through N-h…I hydrogen-bonding interactions, the whole structure represents a quasi-two dimensional arrangement. Electrostatic attraction exists between organic cations and inorganic chains, leading to a so-called organic-inorganic hybrid structure.

关键词： coordination polymer single-crystal diffraction template synthesis iodide

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The regulation of the Treg/Th 17 balance by mesenchyma stem cells in human systemic lupus erythematosus

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Cellular & Molecular Immunology 2017年第5期14卷 423-431页

作者： Dandan Wang Saisai huang xinran Yuan Jun Liang Renju xu Genhong Yao xuebing Feng Lingyun sun Department of Rheumatology and Immunology The Affiliated Drum Tower Hospital of Nanjing University Medical School Nanjing China

Background and objective： Umbilical cord （UC）-derived mesenchymal stem cells （MSCs） have shown immunoregulation of various immune cells. The aim of this study was to investigate the mechanism of UC MSCs in the regulation of peripheral regulatory T cells （Treg） and T helper 17 （Th17） cells in patients with systemic lupus erythematosus （SLE）. Methods： Thirty patients with active SLE, refractory to conventional therapies, were given UC MSCs infusions. The percentages of peripheral blood CD4＋CD25＋Foxp3＋ regulatory T cells （Treg） and CD3＋CD8-1L17A＋ Th17 cells and the mean fluorescence intensities （MFI） of Foxp3 and IL- 17 were measured at I week, I month, 3 months, 6 months, and 12 months after MSCs transplantation （MSCT）. Serum cytokines, including transforming growth factor beta （TGF-β）, tumor necrosis factor alpha （TNF-α）, interleukin 6 （IL-6）, and IL-17A were detected using ELISA. Peripheral blood mononuclear cells from patients were collected and co-cultured with UC MSCs at ratios of 1：1, 10：1, and 50：1, respectively, for 72 h to detect the proportions of Treg and Th17 cells and the MFIs of Foxp3 and IL-17 were determined by flow cytometry. The cytokines in the supernatant solution were detected using ELISA. Inhibitors targeting TGF-β, IL-6, indoleamine 2,3-dioxygenase （IDO）, and prostaglandin E2 were added to the co-culture system, and the percentages of Treg and Th17 cells were observed. Results： The percentage of peripheral Treg and Foxp3 MFI increased 1 week, 1 month, and 3 months after UC MSCs transplantation, while the Th17 proportion and MFI of IL-17 decreased 3 months, 6 months, and 12 months after the treatment, along with an increase in serum TGF-β at I week, 3 months, and 12 months and a decrease in serum TNF-a beginning at I week. There were no alterations in serums IL-6 and IL-17A before or after MSCT. In vitro studies showed that the UC MSCs dose-dependently up-regulated peripheral Treg proportion in SLE patients, which was not depended on cell-cell co

关键词： mesenchymal stem cells regulatory T cells systemic lupus erythematosus T helper 17 cells umbilical cord

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Thermodynamic modeling and elemental migration for the early stage of rodingitization:An example from the xialu massif of the xigaze ophiolite,southern Tibet

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Geoscience Frontiers 2021年第3期12卷 557-576页

作者： Wen-Yong Duan xu-Ping Li Ze-Li Wang Shuang Chen Guang-Ming sun Ling-Quan Zhao Shandong Provincial Key Laboratory of Depositional Mineralization and Sedimentary Minerals Shandong University of Science and TechnologyQingdao 266590China Institute of Geology Mineralogy and GeophysicsFaculty of GeosciencesRuhr-University Bochum44780 BochumGermany

The analysis of early stage rodingite from the ultramafic rocks of the xialu Massif in the xigaze Ophiolite,Tibet,in China shows that the rodingitization involved continuous changes in fluid composition during different stages of *** early stage prehnite-bearing rodingite was produced at low pressures and temperatures along extensional *** of rodingite were collected along a profile from the center to the margin of a rodingitized intrusive igneous rock(^10 m×30 m),and they record wide variations in bulk composition,mineralogy,and *** mineral assemblages,from center to margin,vary from(1)relics of primary clinopyroxene(Cpx_(r))and primary amphibole(Amp_(r))+newly formed late amphibole(Act)+primary plagioclase(Pl_(r))+clinozoisite+prehnite+albite+chlorite+titanite+ilmenite(R1 rodingite),through(2)relics of primary clinopyroxene(Cpx_(r))+newly formed late clinopyroxene(Cpx_(n))+primary and late amphiboles(Amp_(r)+Act)+clinozoisite+prehnite+albite+chlorite+titanite(R2 rodingite),to(3)newly formed late clinopyroxene(Cpx_(n))and amphibole(Act)+clinozoisite+prehnite+albite+chlorite+titanite(R3 rodingite).As a result of the metasomatic process of rodingitization,the content of CaO in the whole rock chemical composition from R1 to R3 increases,SiO_(2) decreases,and Na_(2)O+K_(2)O is almost completely *** diagrams show enrichments in large ion lithophile elements such as Rb,Cs,Ba,and Pb as well as Ni during *** central part of the rodingitized intrusion(R1 rodingite)was only slightly affected by *** the other hand,the contents of the rare earth elements(REEs),high field strength elements(hFSEs;***,Nb,Ta,hf,and Y),and some highly compatible elements such as Cr and Sc decreased slightly during *** modeling based on equilibrium mineral assemblages indicates that the rodingite of the xialu Massif formed in an h_(2)O-saturated,CO_(2)-rich *** estimated conditions o

关键词： Early stage rodingitization Element migration Thermodynamic modeling xialu Massif xigaze Ophiolite Southern Tibet

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马钱子苷通过肠道菌群和神经肽抑制OVx小鼠骨量丢失的机制探讨

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中国骨质疏松杂志 2024年第4期30卷 538-545页

作者：谢佑红周天宇王金枝王姜俨周怡琳唐琳董群伟孙平广东药科大学附属第一医院内分泌科广东广州510006 山东省青岛市即墨区人民医院感染科山东青岛266200 广东药科大学附属第一医院骨科广东广州510006 广东省云浮市中医院广东云浮527300

目的观察马钱子苷(Loganin)干预去卵巢(OVx)小鼠骨量、肠道菌群及神经肽的变化。方法28只雌性C57BL/6J小鼠随机分为假手术组(ShAM)、OVx组、Loganin低剂量组(Loganin-L)和Loganin高剂量组(Loganin-h),每组7只。Loganin-L组和Loganin-h组在OVx基础上分别予Loganin 5 mg/(kg·d)和20 mg/(kg·d)灌胃,ShAM组和OVx组灌胃等量蒸馏水,每日1次,持续12周。实验结束后处死取材,Micro-CT检测左侧股骨BMD、BV/TV、Tb.N及***;ELISA法检测血清中CTx、TRAP、P1NP、TNF-α、IL-6、IL-10、LPS、CGRP、SP、VIP、NPY水平;16 S rRNA高通量测序技术检测粪便中肠道菌群多样性与差异性。结果OVx组BMD、BV/TV、Tb.N、P1NP、IL-10、CGRP、SP、VIP、NPY较ShAM组显著降低(Px、TRAP、TNF-α、IL-6、LPS显著增高(Px组有升高趋势(P>0.05),***、CTx、TRAP、TNF-α、IL-6、LPS有降低趋势(P>0.05)。Loganin-h组BMD、BV/TV、Tb.N、P1NP、IL-10、CGRP、SP、VIP、NPY较OVx组显著增高(Px、TRAP、TNF-α、IL-6、LPS显著降低(PhAM组相比,OVx组Firmicutes/Bacteroidota(F/B)率显著降低(Px组有升高趋势(P>0.05),Loganin-h组F/B率显著升高(Px组norank-f_Muribaculaceae丰度较ShAM组显著增加(Px组,Loganin-L组norank-f_Muribaculaceae丰度有降低趋势(P>0.05),Lactobacillus丰度有升高趋势(P>0.05),Loganin-h组norank-f_Muribaculaceae丰度显著减少(Px小鼠骨量丢失,其机制可能是通过调节肠道菌群中norank-f_Muribaculaceae和Lactobacillus丰度、增加神经肽含量及降低炎症反应实现的。

关键词：中医中药马钱子苷去卵巢小鼠骨质疏松

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Antioxidation of Anthocyanins in Photosynthesis Under high Temperature Stress

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Journal of Integrative Plant Biology 2007年第9期49卷 1341-1351页

作者： Ling Shao Zhan Shu Shu-Lan sun Chang-Lian Peng xiao-Jing Wang Zhi-Fang Lin College of Life Science Guangdong Provincial Key Laboratory of Biotechnology for Plant Development South China Normal University Guangzhou 510631 China Biology Department Zhao Qing University Zhaoqing 526061 China South China Botanical Garden the Chinese Academy of Sciences Guangzhou 510650 China

Chlorophyll fluorescence and antioxidative capability in detached leaves of the wild type Arabidopsis thaliana L. ecotype Landsberg erecta （Ler） and three mutants deficient in anthocyanins biosynthesis （tt3, tt4, and tt3tt4） were investigated during treatment with temperatures ranging 25-45 ℃. In comparison with the wild type, chlorophyll fluorescence parameters Fv/Fm, φps,, electron transport rate （ETR）, Fv/Fo and qP in three anthocyanin-deficient mutants showed a more rapidly decreasing rate when the temperature was over 35 ℃. Non-photochemical quenching （NPQ） in these mutants was almost completely lost at 44 ℃, whereas the content of heat stable protein dropped and the rate of the membrane leakage increased. Fo-temperature curves were obtained by monitoring Fo levels with gradually elevated temperatures from 22 ℃ to 72 ℃ at 0.5 ℃/min. The inflexion temperatures of Fo were 45.8 ℃ in Ler, 45.1℃ in tt3, 44.1℃ in tt4 and 42.3 ℃ in tt3tt4, respectively. The temperatures of maximal Fo in three mutants were 1.9-3.8℃ lower than the wild type plants. Meanwhile, three mutants had lower activities of superoxide dismutase （SOD） and ascorbate peroxidase （APx） and an inferior scavenging capability to DPPh （***） radical under heat stress, and in particular tt3tt4 had the lowest antioxidative potential. The results of the diaminobenzidine-h2O2 histochemical staining showed that h2O2 was accumulated in the leaf vein and mesophyll cells of mutants under treatment at 40 ℃, and it was significantly presented in leaf cells of tt3tt4. The sensitivity of Arabidopsis anthocyanins-deficient mutants to high temperatures has revealed that anthocyanins in normal plants might provide protection from high temperature injury, by enhancing its antioxidative capability under high temperature stress.

关键词： anthocyanins-deficient mutant antioxidative capability Arabidopsis thaliana chlorophyll fluorescence h2O2 histochemical localization high temperature stress.

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Relationship Between Polyamines Metabolism and Cell Death in Roots of Malus hupehensis Rehd. Under Cadmium Stress

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Journal of Integrative Agriculture 2012年第7期11卷 1129-1136页

作者： JIANG Qian-qian YANG hong-qiang sun xiao-li LI Qiang RAN Kun ZhANG xin-rong College of horticulture Science and Engineering Shandong Agricultural University Tai'an 2 71018 P.R. China State Key Laboratory of Crop Biology Shandong Agricultural University Tai'an 271018 P.R.China

The free putrescine （Put） content, the hydrogen peroxide （h202） content and the polyamine oxidase （PAO） activity in roots of Malus hupehensis Rehd. var. pinyiensis Jiang （PYTC） were significantly increased, and reached its peak at 1, 2 and 6 h, respectively, under cadmium treatment. The free spermine （Spin） and spermidine （Spd） contents were dramatically decreased, and reached the minimum value at 4-6 h, then remained relatively stable. The change in total free polyammes （PAs） content was consistent with that of free Put. The number of root dead cells was gradually increased after treatment for 24 h, and the typical characteristics of programmed cell death （PCD） were displayed at 48 h. Throughout the Cd treatment process, changes in PAs metabolism appeared to be prior to cell death increase, and the h2O2 content was always maintained at a high level. These results indicated that polyamines could initiate cell death by generating h2O2 in roots of Malus hupehensis Rehd. under CdSO4 stress.

关键词： Malus hupehensis Rehd. cadmium cell death polyamines metabolism

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