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Synthesis of Long-Chain Oligomeric Donor and Acceptors via Direct Arylation for Organic Solar Cells

Ch.n.se Journal of Chemistry 2024年第5期42卷 523-532页

作者： Yu Wu Xin-Yu he Xu-Min huang Ling-Jun Yang Peng Liu na Ch.n.Ch.n.-Zhi Li Shi-Yong Liu Jiangxi Provincial Key Laboratory of Functional Molecular Materials Chemistry College of MaterialsMetallurgical and ChemistryJiangxi University of Science and TechnologyGanzhouJiangxi341000 China Department of Polymer Science and Engineering Zhejiang UniversityHangzhouZhejiang310027 China

The rapid synthesis of structurally complicated electron donors&acceptors still remains a major challenge in organic solar cells(OSC).In this work,we developed a highly efficient strategy to access long-chain oligomeric donor and acceptors for OSC applications.A series of cyclopentadithioph.n.(CPDT)and benzothiadiazole(BT)-basedπ-conjugated oligomers,i.e.,three oligomeric acceptors(BTDT)n-IC(n=1—3)and one long-chain oligomeric donor(BTDT)4-RD,are facilely synthesized by an atom-and step-economical,and labor-saving direct C—h arylation(DACh)reaction(i.e.,C—h/C—Br cross coupling).note that(BTDT)4-RD involving five CPDT,four BT and two rhodamine(RD)building blocks is the longest oligomeric donor in the fullerene-free OSC devices ever *** dependence of the structure-property-performance correlation of(BTDT)n-IC(n=1—3)and(BTDT)4-RD on theπ-conjugation lengths is thoroughly investigated by opto-electrochemical measurements,bulk heterojunction(BhJ)OSC devices and ***(BTDT)1-IC:PBDB-T and(BTDT)4-RD:Y6 BhJs achieve power conversion efficiencies of 9.14%and 4.51%,*** findings demonstrate that DACh reaction is a powerful tool to tune the opto-electronic properties and device performances by regulating the lengths ofπ-conjugated oligomers with varied numbers of repeating units.

关键词： Long-chainπ-conjugated oligomer Organic solar cells Direct arylation Atom-economical synthesis Cyclopentadithioph.n. Benzothiadiazole C—h activation Conjugation Oligomers

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Inclusion of CoTiO_(3) to ameliorate the re/dehydrogenation properties of the Mg–na–Al system

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Journal of Magnesium and Alloys 2024年第3期12卷 1215-1226页

作者： n.A.Ali n.Y.Yusnizam n.A.Sazelee Sami-ullah Rather haizh.n.Liu M.Ismail Energy Storage Research Group Faculty of Ocean Engineering TechnologyUniversiti Malaysia Terengganu21030 Kuala NerusTerengganuMalaysia Department of Chemical and Materials Engineering King Abdulaziz UniversityP.O.Box80204Jeddah 21589Saudi Arabia Guangxi novel Battery Materials Research Center of Engineering Technology School of Physical Science and TechnologyGuangxi UniversityNanning 530004China State Key Laboratory of Featured Metal Materials and Life-Cycle Safety for Composite Structures Nanning 530004China

For the first time,the Mgh_(2)–naAlh_(4)(ratio 4:1)destabilized system with CoTiO_(3) addition has been *** CoTiO_(3)-doped Mgh_(2)–naAlh_(4) sample begins to dehydrogenate at 130℃,which is declined by 40℃ compared to the undoped Mgh_(2)–naAlh_(4).Moreover,the de/rehydrogenation kinetics characteristics of the CoTiO_(3)-doped Mgh_(2)–naAlh_(4) were greatly *** the inclusion of CoTiO_(3),the Mgh_(2)–naAlh_(4) composite absorbed 5.2 wt.%h_(2),higher th.n.undoped Mgh_(2)–naAlh_(4).In the context of dehydrogenation,the CoTiO_(3)-doped Mgh_(2)–naAlh_(4) sample desorbed 2.6 wt.%h_(2),almost doubled compared to the amount of hydrogen desorbed from the undoped Mgh_(2)–naAlh_(4) *** activation energy obtained by the Kissinger analysis for Mgh_(2) decomposition was significantly lower by 35.9 kJ/mol th.n.the undoped Mgh_(2)–naAlh_(4) *** reaction mech.n.sm demonstrated that new phases of MgCo and AlTi_(3) were generated in situ during the heating process and are likely to play a substantial catalytic function and be useful in ameliorating the de/rehydrogenation properties of the destabilized Mgh_(2)–naAlh_(4) system with the inclusion of CoTiO_(3).

关键词： Destabilize system Magnesium hydride Sodium alanate hydrogen storage Additive.

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甲基化转移酶WTAP调节转录激活因子4表达加剧缺氧/复氧诱导的心肌细胞损伤

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中华危重病急救医学 2024年第3期36卷 279-285页

作者：杨娜曹敏成都市第三人民医院麻醉科四川成都610000 成都市第三人民医院泌尿外科四川成都610000

目的观察甲基化转移酶Wilms肿瘤蛋白1相关蛋白(WTAP)在缺氧/复氧(h/R)诱导的心肌细胞损伤中的调节作用及其分子机制。方法①实验一:将h9C2心肌细胞分为空白对照组和h/R模型组。采用h/R诱导h9C2细胞建立心肌缺血/再灌注(I/R)损伤模型;空... 详细信息

目的观察甲基化转移酶Wilms肿瘤蛋白1相关蛋白(WTAP)在缺氧/复氧(h/R)诱导的心肌细胞损伤中的调节作用及其分子机制。方法①实验一:将h9C2心肌细胞分为空白对照组和h/R模型组。采用h/R诱导h9C2细胞建立心肌缺血/再灌注(I/R)损伤模型;空白对照组不进行处理。采用n6-甲基腺苷(m6A)Rna甲基化测定试剂盒检测m6A水平;分别采用实时荧光定量聚合酶链反应(RT-qPCR)和蛋白质免疫印迹试验(Western blotting)检测甲基化转移酶〔WTAP、甲基转移酶样蛋白(METTL3、METTL14)〕的基因和蛋白表达水平。②实验二:将h9C2心肌细胞分为空白对照组、h/R+sh.n.组、h/R+sh-WTAP组。h/R+sh-WTAP组转染sh-WTAP以敲降WTAP表达,其余各组制模方法同实验一。转染48 h后,采用流式细胞术检测细胞凋亡率;采用Western blotting检测WTAP、活化天冬氨酸特异性半胱氨酸蛋白酶3(caspase-3)、活化多聚二磷酸腺苷酸核糖聚合酶(PARP)、转录激活因子4(ATF4)、蛋白激酶Rna样内质网激酶(PERK)、磷酸化PERK(p-PERK)和CCAAT/增强子结合蛋白同源蛋白(ChOP)的蛋白表达水平;采用免疫荧光染色观察ATF4阳性表达情况。③实验三:将h9C2心肌细胞分为空白对照组、h/R+sh.n.组、h/R+sh-WTAP和h/R+sh-WTAP+ATF4组。h/R+sh-WTAP+ATF4组用过表达质粒ATF4转染h9C2心肌细胞,其余各组制模方法同实验二。采用流式细胞术检测细胞凋亡率;采用Western blotting检测ATF4、ChOP、活化caspase-3和活化PARP的蛋白表达水平。结果①实验一:h/R模型组m6A甲基化水平较空白对照组显著上调。RT-qPCR检测结果显示,h/R模型组METTL3、METTL14和WTAP基因表达水平均较空白对照组显著上调,以WTAP上调最显著;Western blotting检测结果呈相同趋势。提示甲基化转移酶WTAP的表达水平在h/R诱导的心肌细胞中显著上调。②实验二:h/R+sh-WTAP组细胞凋亡水平较h/R+sh.n.组明显减少〔(14.16±1.58)%比(24.51±2.38)%,Pn blotting检测结果显示,h/R+sh-WTAP组WTAP、活化caspase-3、活化PARP、p-PERK、ATF4和ChOP的蛋白表达水平均较h/R+sh.n.组显著下调。荧光显微镜下显示,h/R+sh-WTAP组ATF4阳性信号较h/R+sh.n.组显著减弱〔(19.36±1.81)%比(32.83±2.69)%,Ph/R诱导的心肌细胞凋亡和内质网应激。③实验三:h/R+sh-WTAP+ATF4组细胞凋亡水平较h/R+sh-WTAP组显著增加〔(26.61±2.76)%比(17.14±0.87)%,Pn blotting检测结果显示,h/R+sh-WTAP+ATF4组ATF4、ChOP、活化caspase-3和活化PARP的蛋白表达水平较h/R+sh-WTAP组显著上调。提示过表达ATF4可逆转sh-WTAP对h/R诱导的心肌细胞中内质网应激和细胞凋亡的抑制作用。结论甲基化转移酶WTAP可调节ATF4表达,介导细胞凋亡和内质网应激,促进h/R诱导的心肌细胞损伤。

关键词：甲基转移酶 Wilms肿瘤蛋白1相关蛋白细胞凋亡内质网应激缺氧/复氧

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n-acetylserotonin alleviates retinal ischemia-reperfusion injury via hMGB1/RAGE/nF-κB pathway in rats

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International Journal of Ophthalmology(English edition) 2024年第2期17卷 228-238页

作者： Yu-Ze Zhao Xue-ning Zh.n. Yi Yin Pei-Lun Xiao Meng Gao Lu-Ming Zh.n. Shuan-hu Zhou Shu-na Yu Xiao-Li Wang Yan-Song Zhao Department of Ophthalmology Affiliated Hospital of Weifang Medical UniversityWeifang 261031Shandong ProvinceChina School of Medical Imaging Weifang Medical UniversityWeifang 261053Shandong ProvinceChina Department of Anatomy Weifang Medical UniversityWeifang 261053Shandong ProvinceChina Departments of Orthopedic Surgery Brigham and Women’s HospitalHarvard Medical SchoolBostonMA 02115USA

AIM:To observe the effects of n-acetylserotonin(naS)administration on retinal ischemia-reperfusion(RIR)injury in rats and explore the underlying mech.n.sms involving the high mobility group box 1(hMGB1)/receptor for advanced glycation end-products(RAGE)/nuclear factor-kappa B(nF-κB)signaling ***:A rat model of RIR was developed by increasing the pressure of the anterior chamber of the *** male Sprague Dawley were randomly divided into five groups:sham group(n=8),RIR group(n=28),RIR+naS group(n=28),RIR+FPS-ZM1 group(n=8)and RIR+naS+FPS-ZM1 group(n=8).The therapeutic effects of naS were examined by hematoxylin-eosin(h&E)staining,and retinal ganglion cells(RGCs)*** expression of interleukin 1 beta(IL-1β),hMGB1,RAGE,and nod-like receptor 3(nLRP3)proteins and the phosphorylation of nuclear factorkappa B(p-nF-κB)were analyzed by immunohistochemistry staining and Western blot *** expression of hMGB1 protein was also detected by enzyme-linked immunosorbent assay(ELISA).RESULTS:h&E staining results showed that naS significantly reduced retinal edema and increased the number of RGCs in RIR *** naS therapy,the hMGB1 and RAGE expression decreased significantly,and the activation of the nF-κB/nLRP3 pathway was antagonized along with the inhibition of p-nF-κB and nLRP3 protein ***,naS exhibited an anti-inflammatory effect by reducing IL-1β*** inhibitory of RAGE binding to hMGB1 by RAGE inhibitor FPS-ZM1 led to a significant decrease of p-nF-κB and nLRP3 expression,so as to the IL-1βexpression and retinal edema,accompanied by an increase of RGCs in RIR ***:naS may exhibit a neuroprotective effect against RIR via the hMGB1/RAGE/nF-κB signaling pathway,which may be a useful therapeutic target for retinal disease.

关键词： retinal diseases retinal ischemia—reperfusion injury n-acetylserotonin high mobility group box 1 receptor for advanced glycation end-products nuclear factor-κB rats

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Molecular Cloning of sodB Gene from Vibrio alginolyticus hy9901 and Its Bioinformatics Analysis

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Asian Agricultural Research 2024年第7期16卷 42-47页

作者： Shuai YAnG Yingying JIAnG haiyun FEnG Weijie Zh.n. na WAnG Xiaonan LU huanying PAnG Fisheries College Guangdong Ocean UniversityZhanjiang 524025China Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and healthy Culture&Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong higher Education Institutes Zhanjiang 524025China Ch.n.se Academy of Inspection and Quarantine Beijing100176China Department of Food Science and Agricultural Chemistry Faculty of Agricultural and Environmental SciencesMcGill UniversitySainte-Anne-de-Bellevue H9X 3V9Canada

Vibrio alginolyticus is a zoonotic bacterium.A pair of specific primers was designed using the sodB gene sequence of Vibrio alginolyticus hy9901 in order to amplify the full length of the gene by *** results indicated that the total length of the sodB gene was 585 bp and that it could encode 194 amino *** predicted amino acid sequence derivation indicated that the molecular weight of the protein was approximately 21.56 kDa,with an isoelectric point of *** prediction of the n-terminal signal peptide structure of the protein,no significant signal peptide cleavage site was observed,indicating that the protein lacked both a signal peptide and a transmembrane *** amino acid sequence contained an n-glycosylation site,a casein kinase II phosphorylation site,a microsomal C-terminal target signal site,and a manganese and iron superoxide dismutase signal *** probability of intracytoplasmic localization of the SodB protein was 56.5%,which was analyzed according to the subcellular localization of the *** amino acid sequence of the sodB gene of *** exhibited 98%-100%homology to other Vibrio species,clustering into the same subfamily with ***,indicating a relatively close relationship between *** the prediction of protein structure,the proportions ofα-helix,random coil,β-sheet,and extended strand were 48.45%,30.41%,5.67%,and 15.46%,*** similarity to template 1dt0.1.A reached 71.58%.A PTM site analysis revealed the presence of phosphorylation,glycosylation,ubiquitination,sumoylation,acetylation,and methylation modification sites,as well as the absence of lactylation modification sites.

关键词： Vibrio alginolyticus Gene cloning sodB gene Bioinformatics analysis

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Gene Cloning and Bioinformatics Analysis of phoR Gene from Vibrio alginolyticus hy9901

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Asian Agricultural Research 2024年第6期16卷 36-40页

作者： Xiangyu LIU Peng ZhOU haiyun FEnG Weijie Zh.n. huanying PAnG na WAnG Xiaonan LU Fisheries College Guangdong Ocean UniversityZhanjiang 524025China Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and healthy Culture&Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong higher Education Institutes Zhanjiang 524025China Ch.n.se Academy of Inspection and Quarantine Beijing100176China Department of Food Science and Agricultural Chemistry Faculty of Agricultural and Environmental SciencesMcGill UniversitySainte-Anne-de-Bellevue H9X 3V9Canada

PhoR is a histidine kinase in a two-component regulatory system that regulates phosphorus metabolic pathways and undertakes the key mission of information transmission in pathogenic *** full-length phoR gene was successfully cloned from the Vibrio alginolyticus hy9901 strain.A compreh.n.ive analysis of the cloned gene was conducted using *** analysis revealed that the total length of the phoR gene(GenBank accession no.:KJ958404.1)is 1299 bp,with the coding region containing a total of 432 amino acid *** phylogenetic tree of PhoR revealed that it belongs to the same subclade as *** SMART program was employed for the purpose of functional domain prediction,which revealed that PhoR possesses three major functional domains:PAS(amino acids 98-166),hisKA(amino acids 205-272),and hATPase_c(amino acids 317-429).

关键词： Vibrio alginolyticus phoR gene Gene cloning Bioinformatics analysis

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带有渐近线性项和库伦位势的薛定谔-泊松系统

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数学物理学报（A辑） 2024年第3期44卷 650-660页

作者：常金华魏娜中南财经政法大学统计与数学学院武汉430073

该文研究下列薛定谔-泊松系统{−Δu+(ω−∑_(i=1)^(m)1/|x−xi|)u+λϕ(x)u=f(u),x∈R^(3),−Δϕ=|u|^(2),u∈h^(1)(R^(3))其中ω>0,λ>0,xi∈R^(3),m∈n,f(u)∼lu(u→+∞)是渐近线性项.该文应用变分方法研究参数ω,λ及渐近系数l的取值范... 详细信息

关键词：椭圆型方程渐近线性变分方法

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Janus hydrogel/electrospun-membrane dressing enh.n.ing wound healing in rats

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Biomedical Engineering Communications 2024年第2期3卷 17-25页

作者： Zi-Rui Zhu Jia-na huang Jin-Ze Li hao Cao Zhao-Yi Lin Yan Li School of Biomedical Engineering Shenzhen Campus of Sun Yat-sen UniversityShenzhen 518107China Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument Sun Yat-sen UniversityGuangzhou 510006China

Background:Excessive exudate secreted from chronic wounds often leads to overhydration and *** a variety of dressings are currently available in clinical applications,they frequently fail to provide multifunction to promote chronic wound *** dressings with a Janus structure,featuring distinct properties on each side,are potential to improve wound ***:Composite dressings with a Janus structure were fabricated,comprising freeze-dried polycaprolactone(PCL)electrospun membrane and alginate-based *** PCL fibrous membrane provided air permeability,while the hydrogel loaded with Deferoxamine,composed of alginate and poly(n-isopropylacrylamide)(PnIPAM),exhibited hygroscopic *** inclusion of PnIPAM imparted ***:The hydrogel(thickness of 2.778±0.082 mm)exhibited a robust adhesion to the fiber membrane(thickness of 0.261±0.041 mm).For AnDC(Alginate-PnIPAM hydrogel with Deferoxamine/PCL membrane)samples,the water vapor transmission rate(WVTR)was measured to be 3364.80±23.23 g∙m−2∙day−1 and the swelling ratio at 2 h was determined to be 1179±125%.The thermo-responsivity of AnDC samples manifested in an increased swelling rate,escalating from 797±189%at 37°C to 1132±147%at 4°*** elastic modulus was assessed for lyophilized and rehydrated AnDC *** theωof the rheometer rotor was decreased from 10 rad/s to 0.1 rad/s,the lyophilized dressing exhibited a decrease from 2.65±0.01 MPa to 1.80±0.90 MPa,while the rehydrated dressing demonstrated an increase from 133.65±55.68 Pa to 264.23±141.71 *** pro-healing properties of the dressings were evaluated using full-thickness skin defect model on SD rats,and a circular wound of diameter 10 mm healed completely by day ***:The dressings not only protected the wound and absorbed excess exudate,but also demonstrated nondestructive peelability upon cooling,providing a novel approach for accelerating wound healing and management.

关键词： Janus structure composite dressing wound healing

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血清白细胞介素-6、沉默信息调节因子-1与衰弱的相关性研究

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中华急诊医学杂志 2024年第5期33卷 677-682页

作者：刘慧珍王娜商娜李俊玉刘小蒙郭树彬腾飞首都医科大学康复医学院中国康复研究中心北京博爱医院急诊科北京100068 首都医科大学附属北京朝阳医院急诊医学中心心肺脑复苏北京市重点实验室北京100020

目的研究血清白细胞介素(interleukin,IL)-6、沉默信息调节因子(silent information regulator,SIRT)-1与急诊科老年患者衰弱的相关性。方法本研究为横断面研究,收集2022年1月至12月于北京博爱医院急诊科治疗的60岁及以上患者。入院后2... 详细信息

目的研究血清白细胞介素(interleukin,IL)-6、沉默信息调节因子(silent information regulator,SIRT)-1与急诊科老年患者衰弱的相关性。方法本研究为横断面研究,收集2022年1月至12月于北京博爱医院急诊科治疗的60岁及以上患者。入院后24 h内检测血常规、生化、IL-6水平;同时采集空腹静脉血2 mL,离心后血清放置-80℃储存,采用酶联免疫吸附测定法检测SIRT-1水平。72 h内进行营养风险筛查2002评分,采用Barthel指数进行日常生活能力评定,测量握力。根据Fried衰弱表型(frailty ph.n.type,FP)评分,将患者分为衰弱与无衰弱组。比较衰弱与无衰弱组间临床资料及实验室指标的差异。采用多因素Logistic回归模型分析血清IL-6、SIRT-1与衰弱的相关性;采用受试者工作特征(receiver operating characteristic,ROC)曲线评价血清IL-6、SIRT-1对衰弱的预测能力。结果本研究纳入患者316例,依据Fried FP标准,分为衰弱组(n=156)和无衰弱组(n=160)。单因素分析示,衰弱组血清IL-6[33.3(13.0,69.2)ng/L vs.20.0(9.2,41.3)ng/L,P=0.001]、SIRT-1[(9.98±1.23)μg/L vs.(8.98±1.65)μg/L,Phel指数、握力后,血清IL-6(OR=1.006,95%CI:1.001~1.011,P=0.036)和SIRT-1(OR=1.838,95%CI:1.475~2.290,Pnder the ROC curve,AUC)为0.671(95%CI:0.604~0.738,Png/L;SIRT-1预测衰弱的AUC为0.736(95%CI:0.674~0.799,P<0.001),截点值为9.13μg/L;二者联合模型的AUC为0.765(95%CI:0.707~0.823,P<0.001),敏感度为0.776,特异度为0.726,其预测效能优于单独应用IL-6(Z=2.119,P=0.034)。结论血清IL-6和SIRT-1可作为急诊科老年患者衰弱的独立预测因子。

关键词：白细胞介素-6 沉默信息调节因子-1 衰弱相关性

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右美托咪定上调nLRC3改善大鼠机械通气相关性肺损伤

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中国新药与临床杂志 2024年第4期43卷 291-295页

作者：乐林莉李娜湖北省妇幼保健院麻醉科湖北武汉430070

目的观察右美托咪定对机械通气相关性肺损伤(VILI)大鼠nLR家族含CARD结构域蛋白3(nLRC3)表达的影响。方法36只SD大鼠随机分为正常对照组、模型组和右美托咪定组,每组各12只。接呼吸机机械通气制备VILI模型,右美托咪定组于制模前20 min... 详细信息

目的观察右美托咪定对机械通气相关性肺损伤(VILI)大鼠nLR家族含CARD结构域蛋白3(nLRC3)表达的影响。方法36只SD大鼠随机分为正常对照组、模型组和右美托咪定组,每组各12只。接呼吸机机械通气制备VILI模型,右美托咪定组于制模前20 min静脉注射右美托咪定5μg·kg^(-1),随后以5μg·kg^(-1)·h^(-1)的剂量维持。正常对照组和模型组于制模前20 min静脉持续输注生理盐水0.5 mL·kg^(-1)·h^(-1)。光镜下观察各组大鼠肺组织病理学结果,检测肺组织湿重-干重比。Western blot和qRT-PCR法分别检测肺组织中nLRC3、nLR家族热蛋白结构域包含蛋白3(nLRP3)、凋亡相关斑点蛋白(ASC)、caspase-1、nF-κB p65的蛋白和mRna表达,ELISA法测定血清中白细胞介素(IL)-1β、IL-18和IL-33的浓度。结果与正常对照组相比,模型组肺组织病理损伤严重,肺损伤定量评价指标(IQA)和肺组织湿重-干重比显著增加(PnLRC3蛋白和mRna表达显著下调(PnLRP3、ASC、caspase-1和nF-κB p65蛋白及mRna的表达显著上调(PnLRC3蛋白及mRna表达显著增加(PnLRP3、ASC、caspase-1和nF-κB p65蛋白和mRna的表达显著下调(PnLRC3的表达改善大鼠VILI,其机制可能与抑制nLRP3炎症小体的激活有关。

关键词：右美托咪定机械通气呼吸机相关性肺损伤炎症 nLR家族热蛋白结构域包含蛋白3

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