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A multi-channel chemical sensor and its application in detecting hydrothermal vents

Acta Oceanologica Sinica 2019年第9期38卷 128-134页

作者： Zhen Cai A J mur Luis Jiwan han Kui Wang huawei Qin Ying Ye Ocean College Zhejiang University Zhoushan 316021 China Environmental and Rural Sciences Institute of Biological Aberystwyth University Aberystwyth SY23 3DA UK Key Laboratory of Marine Ecosystem and Biogeochemistry State Oceanic Administration Hangzhou 310012 China Second Institute of Oceanography Ministry of Natural Resources Hangzhou 310012 China Institute of Mechanical Engineering Hangzhou Dianzi University Hangzhou 310018 China

There are well-established chemical and turbidity anomalies in the plumes occurring vicinity of hydrothermal vents, which are used to indicate their existence and locations. We here develop a small, accurate multi-channel chemical sensor to detect such anomalies which can be used in deep-sea at depths of more than 4 000 m. The design allowed five all-solid-state electrodes to be mounted on it and each (apart from one reference electrode) could be changed according to chemicals to be measured. Two experiments were conducted using the chemical sensors. The first was a shallow-sea trial which included sample measurements and in situ monitoring. ph, Eh, CO3^2- and SO4^2- electrodes were utilized to demonstrate that the chemical sensor was accurate and stable outside the laboratory. In the second experiment, the chemical sensor was integrated with ph, Eh, CO3^2- and h2S electrodes, and was used in 29 scans of the seabed along the Southwest Indian Ridge (SWIR) to detect hydrothermal vents, from which 27 sets of valid data were obtained. hydrothermal vents were identified by analyzing the chemical anomalies, the primary judging criteria were decreasing voltages of Eh and h2S, matched by increasing voltages of ph and CO3^2- . We proposed that simultaneous detection of changes in these parameters will indicate a hydrothermal vent. Amongst the 27 valid sets of data, five potential hydrothermal vents were targeted using the proposed method. We suggest that our sensors could be widely employed by marine scientists.

关键词： chemical sensor multi-channel hydrothermal vents detection chemical anomalies SWIR

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Preliminary analysis on roles of metal–organic compounds in the formation of invisible gold

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Acta Geochimica 2021年第6期40卷 1050-1072页

作者： Medet Junussov Mádai Ferenc Kristály Ferenc Tòth Tivadar Fintor Krisztián muránszky Gábor Prekob Ádám hámor-VidóMária Department of Mineralogy and Petrography Institute of Mineralogy and GeologyFaculty of Earth Sciences and EngineeringUniversity of MiskolcMiskolc-Egyetemváros 3515Hungary Institute of Mineralogy and Geology Faculty of Earth Sciences and EngineeringUniversity of MiskolcMiskolc-Egyetemváros 3515Hungary Department of Mineralogy and Geochemistry Faculty of Science and InformaticsUniversity of SzegedAradi vértank tere 1Szeged 6720Hungary Institute of Chemistry Faculty of Material Sciences and EngineeringUniversity of MiskolcMiskolc-Egyetemváros 3515Hungary Department of Geology and Meteorology Institute of Geography and Earth SciencesFaculty of SciencesUniversity of PécsVasvári pál utcaPecs 7622Hungary

The paper comprises new analytical data on the nature and occurrence of gold in solid pyrobitumen,closely associated with the main gold-bearing sulfide arsenic ores of the Bakyrchik gold deposit(Kazakhstan),related to post-collisional magmatic-hydrothermal *** mineralization of the deposit occurs mainly in the form of an“invisible”type of gold in the structures of arsenian pyrite and arsenopyrite,and the form of gold-organic compounds of pyrobitumen in carbonaceous-terrigenous sequences of Carboniferous *** and electron microscopic analysis,Raman and FT-Infrared analysis,mineralogical and three-step sequential extraction analysis(Nh2OhhCl,h2O2,hNO3?hCl)has been carried out using 9 ore samples(from 3 different types of ores)for a comprehensive study of pyrobitumen and sulfide arsenic ores focusing mainly on organic *** sequentially extracted precious metal content of pyrobitumen reaches up to 7 ppm gold and other metals like Ag 4 ppm,Pt 31 ppb,and Pd 26 ppb,forming metal–organic compounds,while arsenic sulfide minerals incorporate 11 ppm gold,39 ppm Ag,0.49 ppm *** enrichment of gold associating with organic matter and sulfide ore minerals was confirmed in this *** matter was active in the migration of gold and the capture of gold by ***,the reductive organic matter agent released gold,most likely for the sulfide arsenic ore *** was a decisive factor in the concentration,transportation,and preservation of gold in the deposit.

关键词： Gold Pyrobitumen Bakyrchik Vitrinite Metal-organic compounds

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A Semi-Field Approach to Testing Botanical Insecticides. Effects of Natural and Analogues Annonaceous Acetogenins on <i>Spodoptera frugiperda</i>Smith (Lepidoptera: Noctuidae)

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Journal of Agricultural Chemistry and Environment 2021年第4期10卷 458-468页

作者： José Ruiz hidalgo María Gabriela murúa Adriana Neske Instituto de Química Orgánica Facultad de Bioquímica Química y Farmacia UNT Tucumán Argentina Instituto de Tecnologia Agroindustrial del Noroeste Argentino (ITANOA) Estación Experimental Agroindustrial Obispo Colombres (EEAOC)-CONICET Las TalitasTucumán Argentina Facultad de Ciencias Naturales e Instituto Miguel Lillo UNT Miguel Lillo San Miguel de Tucumán

Annonaceous acetogenins enclose a large number of biological activities, among which the insecticidal stands out. Spodoptera frugiperda is a pest that affects corn crops among others and has a great capacity to develop resistance to traditional insecticides, which represents sufficient reasons for the search for new alternatives for their control. The objective of this study was to determine the appropriate concentration and screening new natural insecticides against second instar larvae of S. frugiperda, under glasshouse conditions on Zea mays L. Natural products such as annonaceous acetogenins and some acetylated and methoxy methylated ACG derivatives and the commercial product Lambda-cyhalotrin (LC) were evaluated. The percent mortalities of S. frugiperda larvae in glasshouse conditions were recorded after 24, 48 and 72 h of treatment application. The results showed that the acetogenins tested were significantly (P han untreated check. After 72 h of treatment application the highest percent mortalities were obtained with the mixture of two natural products, rolliniastatin-2 (5) (100 μg/mL) + squamocin (6) (100 μg/mL) + LC (50 μg/mL) that proved the most effective and gave (100%), while rolliniastatin-2 (5) at 100 μg/mL alone, gave lowest percent mortality (65%), followed by squamocin (6) at 100 μg/mL (55%) and LC at 50 μg/mL (30%). The acetylated and methoxy methylated ACGs derivatives caused very low mortality (25% - 35%). It is recommended the mixture as a management option of S. frugiperda as a component of integrated pest management. The results allow us to infer that the addition of natural ACGs synergizes the insecticidal activity of the commercial product. Finding a new ecological alternative for insect control.

关键词： Annonaceous Acetogenins Biocides Spodoptera frugiperda

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Modulation of a Specific Pattern of microRNAs, Including miR-29a, miR-30a and miR-34a, in Cultured human Skin Fibroblasts, in Response to the Application of a Biofunctional Ingredient that Protects against Cellular Senescence <i>in Vitro</i>

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Journal of Cosmetics, Dermatological Sciences and Applications 2015年第4期5卷 332-342页

作者： Xianghong Yan Catherine Serre Laurine Bergeron Ludivine mur Valère Busuttil Jean-Marie Botto Nouha Domloge P&G Innovation Godo Kaisha Kobe Japan Ashland Specialty Ingredients Vincience Global Skin Research Center Sophia Antipolis France

Skin aging is a process of structural and compositional remodeling that can be manifested by wrinkling and sagging. Remarkably, the dermis plays a dominant role in the aging process. Recent studies suggest that microRNAs are implicated in the regulation of gene expression during aging. however, studies about age-related microRNAs and how they modulate skin aging remain limited. In the present work, a complex of hydrolyzed natural yeast proteins (Saccharomyces cerevisiae) and hydrolyzed natural soya bean was developed and showed the ability to modulate the expression of telomere-binding protein TRF2, which is a key factor for telomere protection and to prevent cellular senescence in vitro and DNA damage. The aim of the study was to identify microRNAs specifically modulated after application of the ingredient complex to cultured fibroblasts, and their possible involvement in remodeling of the human extracellular matrix and fibroblast senescence. Consequently, human skin fibroblasts were cultured and treated with 1% of the ingredient complex for 48 h before analyzing microRNA modulation by RT-qPCR. The use of bioinformatics allowed us to predict the target genes for modulated microRNAs. Results show that the ingredient complex modulated a pattern of microRNAs including the down-regulation of miR-29a-3p, miR-30a-5p and miR-34a-5p, which are associated with fibroblast senescence and remodeling of the human dermal extracellular matrix. In conclusion, our results indicate that miR-29a-3p, miR-30a-5p and miR-34a-5p possibly represent key microRNAs that impact human fibroblast senescence and remodeling of the dermal extracellular matrix.

关键词： microRNAs qPCR Array Skin Fibroblasts Skin Senescence Telomere Extracellular Matrix Sirtuin 1 Collagen

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Essential residues for the enzyme activity of ATP-dependent murE ligase from Mycobacterium tuberculosis

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Protein & Cell 2010年第11期1卷 1011-1022页

作者： Chandrakala Basavannacharya Paul R.Moody Tulika Munshi Nora Cronin Nicholas h.Keep Sanjib Bhakta Institute for Structural and Molecular Biology Department of Biological SciencesBirkbeckUniversity of LondonLondon WC1E 7HXUK

The emergence of total drug-resistant tuberculosis(TDRTB)has made the discovery of new therapies for tuberculosis *** cytoplasmic enzymes of peptidoglycan biosynthesis have generated renewed interest as attractive targets for the development of new *** of the cytoplasmic enzymes,uridine diphosphate(UDP)-murNAc-tripeptide ligase(murE),catalyses the addition of meso-diaminopimelic acid(m-DAP)into peptidoglycan in Mycobacterium tuberculosis coupled to the hydrolysis of *** of *** murE were generated by replacing K157,E220,D392,R451 with alanine and N449 with aspartate,and truncating the first 24 amino acid residues at the Nterminus of the *** of the specific activity of these proteins suggested that apart from the 24 Nterminal residues,the other mutated residues are essential for *** in Km values for one or more substrates were observed for all mutants,except the N-terminal truncation mutant,indicating that these residues are involved in binding substrates and form part of the active site *** mutant proteins were also tested for their specificity for a wide range of ***,the mutations K157A,E220A and D392A showed hydrolysis of ATP uncoupled from *** ATP hydrolysis rate was enhanced by at least partial occupation of the uridine nucleotide dipeptide binding *** study provides an insight into the residues essential for the catalytic activity and substrate binding of the ATP-dependent murE *** ATPdependent murE ligase is a novel drug target,the understanding of its function may lead to development of novel inhibitors against resistant forms of ***.

关键词： peptidoglycan murE ligase site-directed mutagenesis m-DAP

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1例2岁儿童的双侧掌部皮下结节为深部环状肉芽肿

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世界核心医学期刊文摘（皮肤病学分册） 2005年第7期1卷 24-24页

作者： mur E.C. Fernández C.M. hermosa J.M.h. 李晓莉冯义国 Servicio de Dermatologa HGU Gregorio Maranón.C/Dr. Esquerdo no 47 28007 Madrid Spain

Granuloma annulare is a benign, relatively common dermatosis in childhood. The subcutaneous form is rare, and lesions typically occur on the legs, buttocks and scalp. We report a case of a deep granuloma annulare conf... 详细信息

关键词：环状肉芽肿掌部皮下结节下型

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锻制镍钛记忆合金临床应用初步报告

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上海第二医学院学报 1982年第2期 31-35页

作者：薛淼上海第二医学院附属第九人民医院上海第二医学院附属瑞金医院上海市伤研所上海钢铁研究所上海手术器械六厂

镍钛记忆合金(Nitinol)是美国海军武器实验室(U.S.N. Ordnance Laboratory)在研究镍钛合金时偶然发现的。它具有独特的“形状记忆效应(Shape Memory Effect)”,即此类合金制件,在低于转变温度明显的塑性形变后,经过加热,可以回复到它变形前

关键词：记忆合金骑缝钉临床应用镍钛锻制

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high-Sensitivity Visualization of Ulitrafast Carrier Diffusion by Wide-Field holographic Microscopy

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Ultrafast Science 2023年第5期3卷 43-49页

作者： Martin hörmann Federico Visentin Andrea Zanetta Johann Osmond Giulia Grancini Niek Fvan hulst Matz Liebel Giulio Cerullo Franco V.A.Camargo Dipartimento di Fisica Politecnico di MilanoPiazza L.da Vinci 3220133 MilanoItaly Department of Chemistry and INSTM University of PaviaVia T.Taramelli 1427100 PaviaItaly ICFO-Institut de Ciencies Fotoniques The Barcelona Institute of Science and Technology08860 CastelldefelsBarcelonaSpain ICREA-InstitucióCatalana de Recerca i Estudis Avançats Passeig Lluís Companys 2308010 BarcelonaSpain IFN-CNR Piazza L.da Vinci 3220133 MilanoItaly.

Ultrafast transient microscopy is a key tool to study the photophysical properties of materials in space and time,but current implementations are limited to≈1-μm fields of view,offering no statistical information for heterogeneous ***,we demonstrated wide-field transient imaging based on multiplexed off-axis ***,we perform ultrafast microscopy in parallel around a hundred diffraction-limited excitation spots over a≈60-μm field of view,which not only automatically samples the photophysical heterogeneity of the sample over a large area but can also be used to obtain a 10-fold increase in signal-tonoise ratio by computing an average *** apply our microscope to study the carrier diffusion processes in methylammonium lead bromide *** observe strong diffusion due to the presence of hot carriers during the first picosecond and slower diffusion *** also describe how many-body kinetics can be misleadingly interpreted as strong diffusion at high excitation densities,while at weak excitation,real diffusion is ***,the vast increase in sensitivity offered by this technique benefits the study of carrier transport not only by reducing data acquisition times but also by enabling the measurement of the much smaller signals generated at low carrier densities.

关键词： diffusion excitation transient

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每核子100MeV以下核—核碰撞中集体流的分量的研究

每核子100MeV以下核—核碰撞中集体流的分量的研究

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第八届全国核物理会议

作者：沈文庆 J.Peter G.Bizard D.Cussol R.Brou M.Louvel J.P.Patry R.Regimabart J.C.Steckmeyer J.P.Sullivan B.Tamain E．Crema h.Doubre K．hagel 靳根明 A.Peghaire F．Saint-Laurent Y．Cassagnou R．Legrain C．Le．Brun E．Rosato R．Mac Grath S．G．Jeong S．M．Lee Y．Nagashima T．Nakagawa M．Ogihara J．Kasagi T．Motobayashi 中国科学院上海原子核研究所 LPC CaenISMRAFrance GANIL CaenFrance CEN SaclayFrance LPN NantesFrance Univ.di Napoli Italy Suny Stony BrookUSA Inst.of Physics Univ.of TsukubaJapan Dep.of Physics Tokyo Inst.of TechnologyJapan

用4π探测器 mur 和 TONNEAU 在 CANIL(法国)测量了 E=25,36,45,55,65,85, MeV/UAl(Ar,x)和 E=36,65MeV/uNi(Ar,x)核反应中集体流的横向和径向分量,用 Monte-Carlo 模拟及实验数据比较了决定反应平面的两种方法,即 Danielewicz 方法和...

用4π探测器 mur 和 TONNEAU 在 CANIL(法国)测量了 E=25,36,45,55,65,85, MeV/UAl(Ar,x)和 E=36,65MeV/uNi(Ar,x)核反应中集体流的横向和径向分量,用 Monte-Carlo 模拟及实验数据比较了决定反应平面的两种方法,即 Danielewicz 方法和类弹碎片方法的优劣,发现就是对于周边碰撞,Danielewicz

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