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Utility of a Relatively Affordable In-h.use h.V-1 Genotyping Assay for Drug Resistance Testing among Non B h.V-1 Infected Drug Naive Patients in Nigeria

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Advances in Microbiology 2018年第5期8卷 355-365页

作者： J. A. Anejo-Okopi h. onywera I. O. Abah.A. O. Ebonyi O. O. Agbaji A. P. Agaba S. Oguch. O. S. Olonitola J. A. Idoko Department of Microbiology University of Jos Jos Nigeria AIDS Prevention Initiative in Nigeria Jos University Teaching Hospital Jos Nigeria h.V-Research.Laboratory Kenya Medical Research Institute Kisumu Kenya Department of Ph.rmacy Jos University Teaching Hospital Jos Nigeria Department of Pediatrics University of Jos Jos Nigeria Department Medicine University of Jos Jos Nigeria Department Family Medicine University of Jos Jos Nigeria Department of Microbiology Ahmadu Bello University Zaria Nigeria

Background: Th. introduction of antiretroviral (ARV) in resource-limited settings h.s increased life expectancy among non-B h.V-1 infected individuals. We used a validated In-h.use genotyping assay to ch.racterize non-B h.V-1 and to determine drug resistance mutations among treatment-naive patients. Meth.ds: Plasma samples from 105 h.V-1 infected drug-naive adult patients attending a tertiary h.spital Jos, Nigeria were subjected to h.V-1 RNA extraction, reverse transcription amplification, and population-based sequencing of th. partial pol gene on th. ABI 3130xl genetic analyzer. Subtyping and ph.logenetic analyses were performed by REGA Subtyping Tool v2.0 and MEGA v5.0 respectively. Drug resistance profiles were evaluated according to IAS-USA 2013 drug resistance mutations list. Result: One h.ndred samples (95.2%) were successfully genotyped. Th. distribution of th. non-B h.V-1 subtypes were;CRF02_AG-48%, G-41.0%, CRF06_cpx-6.0%, and A-5.0%. Ten percent of th. isolates h.d at least one major drug resistance mutation in th. pol gene. Th. drug-class specific resistance prevalences were 6.0% for NRTIs;M41L-1.0%, K65KR-1.0%, M184IM-1.0%, M184V-2.0%, and T215ADNT-1%, 8.0% for NNRTIs;K103N-2%, 1.0% for K101E, E138A, G190A, P225h., Y181I, Y188L, Y181C including protease inh.bitors’ Q58E (1.0%). Conclusion: h.V-1 was h.terogeneously distributed;CRF02_AG and G predominate and some known major mutations associated with.NRTIs and NNRTIs were determined. Th. In-h.use assay is suitable for both.ch.racterization of non-B h.V-1 subtypes and detection of drug resistance at a significant lower cost th.n available commercial genotyping assays. Th.s finding underscores th. need to consider use of low-cost In-h.use genotyping assay as an alternative in resource-limited settings with.non-B h.V-1 epidemic.

关键词： h.V-1 Antiretroviral Th.rapy Drug Resistance Genotyping Affordable In-h.use

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