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Effect of Korean Magnolia obovata Extract on Platelet-Derived Growth Factor-Induced Vascular Smooth Muscle Cells

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Chinese Journal of Integrative Medicine 2020年第9期26卷 677-682页

作者： Hyunjhung Jhun Suji Baek Jinwoo Kim Kang-Pa Lee Hun-Young park Won-hwan park Kiwon Lim Jisu Kim Technical Assistance Center Korea Food Research InstituteJeonbuk(55365)Republic of Korea Department of Medical Science School of MedicineKonkuk UniversitySeoulRepublic of Korea Department of Bio-Science College of Natural ScienceDongguk UniversityGyeongbuk(38066)Republic of Korea Physical Activity&Performance Institute Konkuk UniversitySeoul(05029)Republic of Korea Department of Diagnostic College of Korean MedicineDongguk University GoyangGyeonggi-do(10326)Republic of Korea

Objective:To investigate the effects of Korean Magnolia obovata crude extract(KME)on plateletderived growth factor(PDGF)-BB-induced proliferation and migration of vascular smooth muscle cells(VSMCs).Methods:KME composition was analyzed by high-performance liquid chromatography(HPLC).VSMCs were isolated from the aorta of a Sprague-Dawley rat,incubated in serum free-Dulbecco's modified Eagle's medium in the presence or absence of KME(10,30,100,and 300(xg/mL),then further treated with PDGF-BB(10 ng/mL).VSMC proliferation was detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and VSMC migration was determined using the Boyden chamber and scratch wound healing *** blot analysis was used to detect phosphorylation of extracellular signal-regulated protein kinases 1 and 2(p-ERK1/2),protein kinase B(p-Akt),and stress-activated protein kinase/c-Jun NH2-terminal kinase(p-SAPK/JNK).The antimigration and proliferation effects of KME were tested using aortic sprout ***:The HPLC analysis identified honokiol(0.45 mg/g)and magnolol(0.34 mg/g)as the major components of ***(30,100,and 300μg/m L)significantly decreased the proliferation and migration of PDGF-BB-stimulated(10 ng/mL)VSMCs and the PDGF-BB-induced phosphorylation of EKR1/2,Akt,and SAPK/JNK(P<0.05).Furthermore,PDGF-BBinduced VSMCs treated with 300μg/m L of KME showed reduction in aortic sprout ***:KME could inhibit abnormal proliferation and migration of VSMCs by down-regulating the phosphorylation of EKR1/2 and ***,KME might be a functional food for preventing vascular disorders.

关键词： extracellular signal-regulated protein kinases 1 and 2 Akt platelet-derived growth factor-BB smooth muscle cell functional food

Dynamic changes of protein corona compositions on the surface of zinc oxide nanoparticle in cell culture media

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Frontiers of Chemical Science and Engineering 2019年第1期13卷 90-97页

作者： Vo-Van Giau Yoon-Hee park Kyu-hwan Shim Sang-Wook Son Seong-Soo A. An Department of Bionanotechnology and Gachon Medical Research Institute Gachon University and Gachon Medical Research Institute Seongnam Korea Laboratory of Cell Signaling and Nanomedicine Department of Dermatology and Division of Brain Korea 21 Project for Biomedical Science Korea University College of Medicine Seoul Korea

The potential applications of nanomaterials used in nanomedicine as ingredients in drug delivery systems and in other products continue to expand. When nanomaterials are introduced into physiological environments and driven by energetics, they readily associate proteins forming a protein corona (PC) on their surface. This PC could result in an alteration of the nanomatcrial's surface characteristics, affecting their interaction with cells due to conformational changes in adsorbed protein molecules. However, our current understanding of nanobiological interactions is still very limited. Utilizing a liquid chromatography-mass spectroscopy/mass spectroscopy technology and a Cytoscape plugin (ClueGO) approach, we examined the composition of the PC for a set of zinc oxide nanoparticles (ZnONP) from cell culture media typically and further analyzed the biological interaction of identified proteins, respectively. In total, 36 and 33 common proteins were investigated as being bound to ZnONP at 5 min and 60 min, respectively. These proteins were further analyzed with ClueGO, a Cytoscape plugin, which provided gene ontology and the biological interaction processes of identified proteins. Proteins bound to the surface of nanoparticles that may modify the structure, therefore the function of the adsorbed protein could be consequently affect the complicated biological processes.

关键词： ZnONPs nanoparticles protein corona ClueGO

Portal vein stenting as a significant risk factor for biliary stricture in adult living donor liver transplantation

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Hepatobiliary & Pancreatic Diseases International 2016年第5期15卷 480-486页

作者： Min-Ho Shin Deok-Bog Moon Sung-gyu Lee Shin hwang Ki-Hun Kim Chul-Soo Ahn Tae-Yong Ha Gi-Won Song Dong-hwan Jung Gil-Chun park Young-In Yun Wan-Jun Kim Woo-Hyoung Kang Seok-hwan Kim Gi-Young Ko Division of Hepatobiliary Surgery and Liver Transplantation Department of SurgeryAsan Medical CenterUniversity of Ulsan College of Medicine88 0lympic-ro 43 gilSongpa-guSeoul 05505Korea Department of Radiology and Research Institute of Radiology Asan Medical CenterUniversity of Ulsan College of Medicine88 0lympic-ro 43 gilSongpa-guSeoul 05505Korea

BACKGROUND: Although perioperative portal vein (PV) stent implantation is an effective treatment for steno-occlusive disease in adult living donor liver transplantation (LDLT) re-cipients, we experienced high incidence of biliary anastomotic strictures (BAS) after PV stenting. In this study, we sought to clarify the relation between BAS and PV stenting and to suggest the possible mechanism of BAS and measures to reduce its in-cidence. METHODS: We retrospectively analyzed 44 LDLT recipients who underwent PV stent implantation across the line of PV anastomosis regardless of the location of steno-occlusion (stent group) and their matched controls (non-stented LDLT recipients, n=131). RESULTS: The incidence of BAS was higher in patients in the stent group than that in the control group (43.2% vs 17.6%, P=0.001). Cumulative 6-month and 1-, 2- and 5-year BAS rates were 31.8%, 34.1%, 41.4% and 43.2%, respectively, in the stent group and 13.0%, 13.8%, 16.1% and 17.8%, respectively, in the control group (P=0.001). Multivariate analysis revealed that PV stenting was an independent risk factor for BAS. CONCLUSIONS: Although PV stent implantation is a reliable treatment modality for steno-occlusive PV in adult LDLT recipients, innovative methods to prevent the PV stent from crossing the line of PV anastomosis may be necessary to reduce the incidence of postoperative BAS.

关键词： portal vein stenting biliary stricture living donor liver transplantation

Image-guided conservative management of right colonic diverticulitis

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World Journal of Gastroenterology 2009年第46期15卷 5838-5842页

作者： Sun Jin park Sung II Choi Suk hwan Lee Kil Yeon Lee Department of Surgery Kyung Hee University School of Medicine

AIM： To study the clinical outcomes of medical therapy in patients with right colonic diverticulitis. METHODS： The records of 189 patients with right colonic diverticulitis which was finally diagnosed by computed tomography, ultrasonography, or operative findings were retrospectively reviewed. RESULTS： Of the 189 patients hospitalized for right colonic diverticulitis, the stages of diverticulitis by a modified Hinchey classification were 26 patients （13.8%） in stage 0, 139 patients （73.5%） in stage I a, 23 patients （12.2%） in stage I b, and 1 patient （0.5%） in stage Ⅲ. Medical therapy was undertaken in 185 of 189 patients （97.9%）. One hundred and eighty three of 185 patients were successfully treated with bowel rest and antibiotics. Two patients in stage I b required a resection or surgical drainage because of an inadequate response to conservative treatment. Recurrent diverticulitis developed in 15 of 183 patients （8.2%） who responded to medical therapy. All 15 patients who suffered a second attack had uncomplicated diverticulitis, and were successfully treated with medical ***： Our results indicate that right colonic diverticulitis is essentially benign and image-guided conservative treatment is primarily required.

关键词： Ascending colon Cecum Medical therapy Colonic diverticulitis

TP-MobNet: A Two-pass Mobile Network for Low-complexity Classification of Acoustic Scene

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Computers, Materials & Continua 2022年第11期73卷 3291-3303页

作者： Soonshin Seo Junseok Oh Eunsoo Cho Hosung park gyujin Kim Ji-hwan Kim NAVER Corporation Seongnam13561Korea Department of Computer Science and Engineering Sogang UniversitySeoul04107Korea

Acoustic scene classification(ASC)is a method of recognizing and classifying environments that employ acoustic *** ASC approaches based on deep learning have been developed,with convolutional neural networks(CNNs)proving to be the most reliable and commonly utilized in ASC systems due to their suitability for constructing lightweight *** using ASC systems in the real world,model complexity and device robustness are essential *** this paper,we propose a two-pass mobile network for low-complexity classification of the acoustic scene,named *** inverse residuals and linear bottlenecks,TPMobNet is based on MobileNetV2,and following mobile blocks,coordinate attention and two-pass fusion approaches are *** log-range dependencies and precise position information in feature maps can be trained via coordinate *** capturing more diverse feature resolutions at the network’s end sides,two-pass fusions can also train ***,the model size is reduced by applying weight quantization to the trained *** adding weight quantization to the trained model,the model size is also *** TAU Urban Acoustic Scenes 2020 Mobile development set was used for all of the *** has been confirmed that the proposed model,with a model size of 219.6 kB,achieves an accuracy of 73.94%.

关键词： Acoustic scene classification low-complexity device robustness two-pass mobile network coordinate attention weight quantization

Effect of zeaxanthin on porcine embryonic development during in vitro maturation

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The Journal of Biomedical Research 2017年第2期31卷 154-161页

作者： Seo-jin park Kyoung-Ha So Sang-hwan Hyun Laboratory of Veterinary Embryology and Biotechnology Veterinary Medical Center and College of Veterinary Medicine Chungbuk National University Seowon-gu Cheonglu 28644 Republic of Korea Institute of Stem Cell & Regenerative Medicine Chungbuk National University Seowon-gu Cheongiu 28644 Republic of Korea

Zeaxanthin is a common carotenoid, which is a powerful antioxidant that protects against damage caused by reactive oxygen species. The aim of the present study was to investigate the effects of zeaxanthin supplementation on in vitro maturation of porcine embryo development. We investigated nuclear maturation, intracellular glutathione （GSH）, and reactive oxygen species （ROS） levels during in vitro maturation, and subsequent embryonic development following parthenogenetic activation and in vitro fertilization OVF）. The oocytes were maturated and used at the metaphase II stage. After 42 hours of in vitro maturation, the zeaxanthin-treated group （0.5 μmol/L） showed significant increases in nuclear maturation （89.6%） than the control group （83.4%）（P 〈 0.05）. The intracellular GSH levels increased significantly （P 〈 0.05） as zeaxanthin concentrations increased; ROS generation levels decreased with increased zeaxanthin concentrations, but there were no significant differences. There were no significant differences in subsequent embryonic development, cleavage rate, blastocyst stage rate, and total blastocyst cell numbers following parthenogenetic activation and IVF when in vitro maturation media was supplemented with zeaxanthin. These results suggest that treatment with zeaxanthin during in vitro maturation improved the nuclear maturation of porcine oocytes by increasing the intracellular GSH level, thereby slightly decreasing the intracellular ROS level.

关键词： in vitro maturation porcine embryonic development zeaxanthin antioxidant

Single port laparoscopic right hemicolectomy with D3 dissection for advanced colon cancer

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World Journal of Gastroenterology 2010年第2期16卷 275-278页

作者： Sung Il Choi Kil Yeon Lee Sun Jin park Suk-hwan Lee Department of Surgery School of MedicineKyung Hee University#1 Hoegi-dong Dongdaemun-guSeoul 130-702 South Korea

We report the first case of single port laparoscopic right hemicolectomy for advanced colon *** abdominal 3 cm length incision was made via the umbilicus.A small wound retractor and a surgical glove were used as a single *** soft tissue anterior to the superior mesenteric vein was completely removed and D3 lymph node dissection was *** total operative time was 180 min with minimal blood loss (<50 mL).The size of the tumor was 5 cm×3 cm and its tumor stage was ***-nine lymph nodes were harvested and none were *** believe that single port surgery for colon cancer is a feasible and safe procedure with surgical results comparable to conventional laparoscopic procedures.

关键词： Single port laparoscopic surgery Colon cancer D3 lymph node dissection

Accumulation of poly(adenosine diphosphate-ribose)by sustained supply of calcium inducing mitochondrial stress in pancreatic cancer cells

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World Journal of Gastroenterology 2022年第27期28卷 3422-3434页

作者： Keun-Yeong Jeong Jae Jun Sim Minhee park hwan Mook Kim Research and Development Metimedi PharmaceuticalsIncheon 22006South Korea

BACKGROUND The biochemical phenomenon defined as poly adenosine diphosphate(ADP)-ribosylation(PARylation)is essential for the progression of pancreatic ***,the excessive accumulation of poly ADP-ribose(PAR)induces apoptosis-inducing factor(AIF)release from mitochondria and energy deprivation resulting in the caspase-independent death of cancer *** To investigate whether sustained calcium supply could induce an anticancer effect on pancreatic cancer by PAR *** Two pancreatic cancer cell lines,AsPC-1 and CFPAC-1 were used for the *** influx and mitochondrial reactive oxygen species(ROS)were observed by fluorescence *** in enzyme levels,as well as PAR accumulation and energy metabolism,were measured using assay ***-dependent cell death was investigated followed by confirming in vivo anticancer effects by sustained calcium *** Mitochondrial ROS levels were elevated with increasing calcium influx into pancreatic cancer ***,excess PAR accumulation,decreased PAR glycohydrolase and ADP-ribosyl hydrolase 3 levels,and energy deprivation were *** vitro and in vivo antitumor effects were confirmed to accompany elevated AIF *** This study visualized the potential anticancer effects of excessive PAR accumulation by sustained calcium supply on pancreatic cancer,however elucidating a clear mode of action remains a challenge,and it should be accompanied by further studies to assess its potential for clinical application.

关键词： Pancreatic cancer Calcium Reactive oxygen species Poly adenosine diphosphate-ribose Poly adenosine diphosphate-ribosylation Poly adenosine diphosphate-ribose polymerase Apoptosis-inducing factor Nicotinamide adenine dinucleotide Anticancer effect

Construction of random sheared fosmid library from Chinese cabbage and its use for Brassica rapa genome sequencing project

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Journal of Genetics and Genomics 2011年第1期38卷 47-53页

作者： Tae-Ho park Beom-Seok park Jin-A Kim Joon Ki Hong Mina Jin Young-Joo Seol Jeong-hwan Mun Genomics and Functional Bio-Material Division National Academy of Agricultural ScienceRural Development Administration The Department of Horticultural Science Daegu University

As a part of the Multinational Genome Sequencing Project of Brassica rapa, linkage group R9 and R3 were sequenced using a bacterial artificial chromosome （BAC） by BAC strategy. The current physical contigs are expected to cover approximately 90% euchromatins of both chromosomes. As the project progresses, BAC selection for sequence extension becomes more limited because BAC libraries are restriction enzyme-specific. To support the project, a random sheared fosmid library was constructed. The library consists of 97536 clones with average insert size of approximately 40 kb corresponding to seven genome equivalents, assuming a Chinese cabbage genome size of 550 Mb. The library was screened with primers designed at the end of sequences of nine points of scaffold gaps where BAC clones cannot be selected to extend the physical contigs. The selected positive clones were end-sequenced to check the overlap between the fosmid clones and the adjacent BAC clones. Nine fosmid clones were selected and fully sequenced. The sequences revealed two completed gap filling and seven sequence extensions, which can be used for further selection of BAC clones confirming that the fosmid library will facilitate the sequence completion of B. rapa.

关键词： Brassica rapa Chinese cabbage Fosmid library Genome sequencing Physical contig