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Identification and immune analysis of antimicrobial peptides from the cigarette beetle(Lasioderma serricorne)

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Insect Science 2024年第4期31卷 1121-1134页

作者： Yue Zhang Yi Yan guy Smagghe Hong Yang Ren-huai Dai Wen-jia Yang Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region Institute of EntomologyGuizhou UniversityGuiyangChina Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region College of Biology and Environmental EngineeringGuiyang UniversityGuiyangChina

Antimicrobial peptides(AMPs)in insects are endogenous peptides that are effector components of the innate defense system of the *** may serve as antimicrobial agents because of their small molecular weight and broad-spectrum antimicrobial *** this study,we performed transcriptome analysis of cigarette beetle(Lasioderma serricorne)larvae,parasitized by the ectoparasitic wasp,Anisopteromalus *** AMP genes were significantly upregulated following *** parasitism,postulating the hypothesis that the parasitization enhanced the host's resistance against pathogenic microorganisms through the regulation of host AMP ***,3 AMP genes(LsDef1,LsDef2,and LsCole)were significantly upregulated and we studied their immune function in *** challenge and functional analysis showed that LsCole was responsible for the immune response against Gram-negative and Gram-positive bacteria,while LsDef1 and LsDef2 were involved in insect defense against Gram-positive *** recombinant LsCole exhibited antimicrobial activities against the Gram-negative bacterium Escherichia coli and the Gram-positive bacterium Staphylococcus ***2 showed an antibacterial effect against *** and LsDef2 exhibited antibiofilm activity against *** 2 AMPs disrupted cell membranes and caused leakage of *** cell *** results indicated that the 3 AMPs in *** are involved in the innate immunity of this pest *** AMPs may have potential as antimicrobial agents for bacterial infection ***,data are discussed in relation to new control strategies with greater biosafety against pest insects with use of microbial biocontrol agents in combination with RNA interference against the insect's defensive AMP genes.

关键词： antimicrobial activity antimicrobial peptides innate immune response Lasioderma serricorne RNA interference

Air traffic monitoring using optimized ADS-B CubeSat constellation

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Astrodynamics 2024年第1期8卷 189-208页

作者： Ghulam Jaffer Rameez AMalik Elias Aboutanios Nazish Rubab Ronnie Nader Hans U.Eichelberger guy A.E.Vandenbosch Interdisciplinary Centre for Security Reliability and Trust(SnT)University of LuxembourgLuxembourg1855Luxembourg

The primary technique used for air traffic surveillance is ***,nowadays,its role in surveillance is gradually being replaced by the recently adopted Automatic Dependent Surveillance-Broadcast(ADS-B).ADS-B offers a higher accuracy,lower power consumption,and longer range than radar,thus providing more safety to *** coverage of terrestrial radar and ADS-B is confined to continental parts of the globe,leaving oceans and poles uncovered by real-time surveillance *** study presents an optimized Low-Earth Orbit(LEO)-based ADS-B constellation for global air traffic surveillance over intercontinental trans-oceanic flight *** optimization algorithm is based on performance evaluation parameters,i.e.,coverage time,satellite availability,and orbit stability(precession and perigee rotation),and communication *** results indicate that the constellation provides ample coverage in the simulated global oceanic *** constellation is a feasible and cost-effective solution for global air supervision,which can supplement terrestrial ADS-B and radar systems.

关键词： constellation automatic dependent surveillance-broadcast (ADS-B) air traffic surveillance precession perigee rotation.

Heatwave exacerbates air pollution in China through intertwined climate-energy-environment interactions

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Science Bulletin 2024年第17期69卷 2765-2775页

作者： Tianshu Chen Tao Wang Likun Xue guy Brasseur Department of Civil and Environmental Engineering The Hong Kong Polytechnic UniversityHong Kong 999077China Environment Research Institute Shandong UniversityQingdao 266237China Big Data Research Center for Ecology and Environment Shandong UniversityQingdao 266237China Environmental Modelling Group Max Planck Institute for MeteorologyHamburg 20146Germany National Center for Atmospheric Research BoulderCO 80307USA

Climate change is increasing the frequency and intensity of heatwaves, raising concerns about their detrimental effects on air quality. However, a role for heatwave-human-environment interactions in air pollution exacerbation has not been established. In the summer of 2022, record-breaking heatwaves struck China and Europe. In this study, we use integrated observational data and machine learning to elucidate the formation mechanism underlying one of the most severe ozone pollution seasons on record in central eastern China, an area that encompasses approximately half of China's total population and sown *** findings reveal that the worsened ozone and nitrogen dioxide pollution resulted from a mismatch between energy demand and supply, which was driven by both heatwaves and energy policy-related factors. The observed adverse heatwave-energy-environment feedback loop highlights the need for the diversification of clean energy sources, more resilient energy structures and power policies, and further emission control to confront the escalating climate challenge in the future.

关键词： Heatwaves Air pollution Ozone Energy Machine learning

Genomics,transcriptomics,and peptidomics of the greater wax moth Galleria mellonella neuropeptides and their expression in response to lead stress

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Insect Science 2024年第3期31卷 773-791页

作者： Li-Lin Luo Yang Lin Jun-Hong Linghu Wei Gong Yuan-Hong Luo Man Liu Dao-Chao Jin guy Smagghe Tong-Xian Liu Shun-Hua Gui Tian-Ci Yi Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region Institute of EntomologyGuizhou UniversityGuiyangGuizhouChina Guizhou Institute of Biology Guizhou Academy of SciencesGuiyangChina Institute of Plant Health and Medicine Guizhou UniversityGuiyangChina

Neuropeptides are crucial in regulation of a rich variety of developmental,physiological,and behavioral functions throughout the life cycle of *** an integrated approach of multiomics,we identified neuropeptide precursors in the greater wax moth Galleria mellonella,which is a harmful pest of honeybee hives with a worldwide ***,a total of 63 and 67 neuropeptide precursors were predicted and annotated in the *** genome and transcriptome,in which 40 neuropeptide precursors were confirmed in the *** ***,we identified 12 neuropeptide precursor genes present in *** but absent in honeybees,which may be potential novel pesticide target *** hives were contaminated with heavy metals such as lead,enabling its bioaccumulation in *** bodies through the food chain,we performed transcriptome sequencing to analyze the effects of Pb stress on the mRNA expression level of *** neuropeptide *** treatment by Pb,the expression of neuropeptide F1 was found to be significantly downregulated,implying that this neuropeptide might be associated with responding to the heavy metal stress in *** study comprehensively identified neuropeptide precursors in ***,and discussed the effects of heavy metals on insect neuropeptides,with the example of *** results are valuable for future elucidation of how neuropeptides regulate physiological functions in *** and contribute to our understanding of the insect's environmental plasticity and identify potential new biomarkers to assess heavy metal toxicity in insects.

关键词： Galleria mellonella genomics heavy metal stress neuropeptide peptidomics transcriptomics

The Peripheral Hospital as Focal Point for Pacemaker Activity: Review of the Last 300 Implantations Carried out at the Haute Correze Hospital Center

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World Journal of Cardiovascular Diseases 2024年第9期14卷 547-556页

作者： Mazou Temgoua Alain Berenfeld Lionel Blasco benoit guy-Moyat Cardiology Department Haute Correze Hospital Center Ussel France Cardiac Arrythmia Unit Cardiology Department Dupuytren University Hospital Limoges France

Background: Pacemaker implantation is a very old activity which has revolutionized the cardiology practice throughout the world. This activity is effective at the Haute Correze Hospital Center since more than 20 years. Due to progress in this area, and the increasing request within this center located at the outskirts of town, we set out to evaluate our pacemaker activity in general and more specifically to assess the post-procedural complications in our series patients. Methodology: This was a retrospective longitudinal study. Data were recorded for period of 90 months from 27/05/2016 to 19/11/2023. This data collection was possible via a specific register completed by computerized patient data from the SillageTM software. All files of patients implanted with single or dual chamber pacemakers were included, generator replacements, upgrading procedures and addition of leads were excluded. The sampling was non-probabilistic, consecutive and non-exhaustive. Statistical analysis was carried out using the Excel 2019 spreadsheet and SPSS version 23 software. The quantitative variables were presented as mean ± standard deviation, the qualitative data as proportions. Results: A total of 303 first-time pacemaker’s implantations were carried out during the study period (rate of 40 per year). The average age in the population was 79.7 ± 9.4 years (44 - 99 years) with a male predominance of 63.7% (n = 193). Atrioventricular block (2nd and 3rd degree) was the main indication for pacemaker implantation in 42.9% of cases (n = 130). Patients were most often implanted with a dual-chamber pacemaker (57.7%, n = 175). The approach was most often cephalic in 72.6% of cases (n = 220), followed by the subclavian access in 27.4% of cases (n = 84). The average fluoroscopy time was 7.9 min ± 2.4 (1 - 43). The average irradiation dose in gray/cm2 was 12.4 ± 9.3 (0.22 - 117.5). The average length of hospitalization was 7 ± 4 (2 - 26) days. The overall complication rate at one year was 12.9% (

关键词： Peripheral Hospital Pacemaker Haute Correze Hospital Center

Contribution of the MERISE-Type Conceptual Data Model to the Construction of Monitoring and Evaluation Indicators of the Effectiveness of Training in Relation to the Needs of the Labor Market in the Republic of Congo

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Open Journal of Applied Sciences 2024年第8期14卷 2187-2200页

作者： Roch Corneille Ngoubou Basile guy Richard Bossoto Régis Babindamana Faculty of Science and Technology of Marien Ngouabi University Brazzaville Republic of The Congo

This study proposes the use of the MERISE conceptual data model to create indicators for monitoring and evaluating the effectiveness of vocational training in the Republic of Congo. The importance of MERISE for structuring and analyzing data is underlined, as it enables the measurement of the adequacy between training and the needs of the labor market. The innovation of the study lies in the adaptation of the MERISE model to the local context, the development of innovative indicators, and the integration of a participatory approach including all relevant stakeholders. Contextual adaptation and local innovation: The study suggests adapting MERISE to the specific context of the Republic of Congo, considering the local particularities of the labor market. Development of innovative indicators and new measurement tools: It proposes creating indicators to assess skills matching and employer satisfaction, which are crucial for evaluating the effectiveness of vocational training. Participatory approach and inclusion of stakeholders: The study emphasizes actively involving training centers, employers, and recruitment agencies in the evaluation process. This participatory approach ensures that the perspectives of all stakeholders are considered, leading to more relevant and practical outcomes. Using the MERISE model allows for: • Rigorous data structuring, organization, and standardization: Clearly defining entities and relationships facilitates data organization and standardization, crucial for effective data analysis. • Facilitation of monitoring, analysis, and relevant indicators: Developing both quantitative and qualitative indicators helps measure the effectiveness of training in relation to the labor market, allowing for a comprehensive evaluation. • Improved communication and common language: By providing a common language for different stakeholders, MERISE enhances communication and collaboration, ensuring that all parties have a shared understanding. The study’s appr

关键词： MERISE Conceptual Data Model (MCD) Monitoring Indicators Evaluation of Training Effectiveness Training-Employment Adequacy Labor Market Information Systems Analysis Adjustment of Training Programs Employability Professional Skills

Design Optimization of a Lattice Tower: Structure and Foundations

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Open Journal of Applied Sciences 2024年第2期14卷 483-493页

作者： Cossi Télesphore Nounangnonhou guy Clarence Semassou Kossoun Alain Tossa Noémie Sintondji Laboratory of Energetics and Applied Mechanics (LEMA) University of Abomey-Calavi Abomey-Calavi Benin Laboratory of Electrotechnics Telecommunications and Applied Informatics (LETIA) University of Abomey-Calavi Abomey-Calavi Bénin

Produced in power plants, electrical energy is transported to places of consumption via the electricity network. At the heart of this network are the supports that allow electricity to be efficiently transported over long distances, guaranteeing the security and supply of energy to the various centers of use. In the construction of a line, supports occupy an important part in terms of safety and construction cost. It is therefore essential to optimize their use to reduce the cost of transmission lines. This work addresses this problem, which focuses on the optimal utilization of X-lattice towers in the construction of overhead power lines. The challenge is to reconcile the search for optimal cost and respect for the design, resistance and service constraints of the structure. To do this, a parameter having a strong correlation with the weight, foundation and construction cost of the X-lattice tower for 161 kV lines is determined as an important cost variable. This parameter is the wheelbase of the towers. The junction point between the structure and the foundations is obtained by measuring the forces at the base of the tower following the lowering of the loads. These efforts make it possible to size foundations which are of the inverted or isolated sole type. The results obtained reveal that from 8 meters in width, the wheelbase gradually changes until the optimum is obtained at 6.29 meters. With this wheelbase, the production cost is optimal. It clearly emerges from this study that the construction of lattice pylons with a wheelbase of approximately 6.29 meters makes it possible to optimize the cost of construction of 161 kV lines in the Republic of benin.

关键词： Lattice Tower Wheelbase Cost Optimization Foundation

Projected impacts of climate change on the habitat of Xerophyta species in Africa

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Plant Diversity 2024年第1期46卷 91-100页

作者： Vincent Okelo Wanga Boniface KNgarega Millicent Akinyi Oulo Elijah Mbandi Mkala Veronicah Mutele Ngumbau guy Eric Onjalalaina Wyclif Ochieng Odago Consolata Nanjala Clintone Onyango Ochieng Moses Kirega Gichua Robert Wahiti Gituru Guang-Wan Hu CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical GardenChinese Academy of SciencesWuhan 430074China University of Chinese Academy of Sciences Beijing 100049China Sino-Africa Joint Research Center Chinese Academy of SciencesWuhan 430074China Centre for Integrative Conservation Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglun 666303China Botany Department Jomo Kenyatta University of Agriculture and TechnologyNairobiKenya East African Herbarium National Museums of KenyaP.O.Box 451660-0100NairobiKenya

Climate change poses a serious long-term threat to *** effectively reduce biodiversity loss,conservationists need to have a thorough understanding of the preferred habitats of species and the variables that affect their ***,predicting the impact of climate change on speciesappropriate habitats may help mitigate the potential threats to biodiversity ***,a monocotyledonous genus of the family Velloziaceae is native to mainland Africa,Madagascar,and the Arabian *** key drivers of Xerophyta habitat distribution and preference are *** 308 species occurrence data and eight environmental variables,the MaxEnt model was used to determine the potential distribution of six Xerophyta species in Africa under past,current and future climate change *** results showed that the models had a good predictive ability(Area Under the Curve and True Skill Statistics values for all SDMs were more than 0.902),indicating high accuracy in forecasting the potential geographic distribution of Xerophyta *** main bioclimatic variables that impacted potential distributions of most Xerophyta species were mean temperature of the driest quarter(Bio9)and precipitation of the warmest quarter(Bio18).According to our models,tropical Africa has zones of moderate and high suitability for Xerophyta taxa,which is consistent with the majority of documented species *** habitat suitability of the existing range of the Xerophyta species varied based on the climate scenario,with most species experiencing a range loss greater than the range gain regardless of the climate *** projected spatiotemporal patterns of Xerophyta species help guide recommendations for conservation efforts.

关键词： Africa Climate change MaxEnt model Potential suitable distribution Velloziaceae Xerophyta

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美国企业计划在月球开采氮-3

国际航空 2024年第4期 68-69页

作者： guy Norris 不详

氢-3是一种珍贵的同位素,可以用于核聚变反应堆的燃料,是一种清洁能源。据信,月球上有大量的这种稀有元素,这使得它获得了太空采矿企业的青睐。当前,氯-3的价格非常昂贵,而且随着清洁能源需求不断增加,其需求还在不断增长。因此,从月球... 详细信息

氢-3是一种珍贵的同位素,可以用于核聚变反应堆的燃料,是一种清洁能源。据信,月球上有大量的这种稀有元素,这使得它获得了太空采矿企业的青睐。当前,氯-3的价格非常昂贵,而且随着清洁能源需求不断增加,其需求还在不断增长。因此,从月球土壤中提取氨一3成为一种颇有吸引力的业务。

关键词：核聚变反应堆采矿企业月球土壤清洁能源稀有元素同位素吸引力