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Bull spermatozoa selected by thermotaxis exhibit high DnA integrity,specific head morphometry,and improve ICSI outcome

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Journal of Animal Science and Biotechnology 2023年第3期14卷 1001-1011页

作者： Sara Ruiz-Diaz Rosane Mazzarella Paula navarrete-Lopez Raul Fernandez-Gonzalez Celia de Frutos Maria Maroto Claudia Cucala Paula Beltran-Brena marta Lombo Dimitrios Rizos Alfonso Gutierrez-Adan Department of Animal Reproduction INIA-CSIC28040 MadridSpain Mistral Fertility Clinics S.L Clinica TambreCalle Tambre828002 MadridSpain

Background Sperm migration by thermotaxis is a guidance mechanism that operates along the oviduct and it has proved to be a valid method for selecting spermatozoa with low DnA fragmentation(SDF)in mice,humans,and *** study aimed to analyse if bull spermatozoa could be selected by thermotaxis and to assess their quality in terms of SDF as well as determine the presence of a specific sperm subpopulation based on sperm morphometry and assess their fertilizing capacity by *** We used frozen-thawed sperm from 6 bulls and sperm selection by thermotaxis was performed with TALP medium supplemented with 25 mmol/L of HEPES and 5 mmol/L of *** these conditions,sperm selection was achieved,obtaining a net thermotaxis of 3.6%.Subsequently,we analysed the SDF of the migrated and not-migrated spermatozoa using the neutral COMET assay,and we evaluated the size of the sperm head using Hemacolor■ staining with Motic Images Plus 3 ***,migrated and not-migrated spermatozoa by thermotaxis were used to fertilize bovine in vitro matured(IVM)oocytes by ICSI,a very inefficient procedure in cattle that is only successful when the oocyte is artificially *** The results showed lower SDF(χ^(2),Pn,n=8)and lower head size parameters(length and width,Pnd perimeter and area,Pn=4)in those spermatozoa migrated in comparison to those *** distribution of sperm subpopulations structure varied between groups,highlighting cluster 2,characterized by spermatozoa with small head size,and high ellipticity and elongated heads,as the most abundant in the thermotaxis migrated *** performed ICSI(without oocyte artificial activation)with the thermotactic sperm,the blastocyst rate was 32.2%±9.3%in the group microinjected with the thermotactic spermatozoa vs.8.3%±7.8%in the group of not-migrated sperm(χ^(2),Pnclusion Our results showed that bull sperm selection by thermotaxis has a much higher D

关键词： ART Bovine Sperm morphometry Sperm selection Thermotaxis

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Acid-mediated decarboxylative C–H coupling between arenes and O-allyl carbamates

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Organic Chemistry Frontiers 2022年第6期9卷 1711-1718页

作者： Camilla Loro Julie Oble Francesca Foschi marta Papis Egle MBeccalli Sabrina Giofrè Giovanni Poli Gianluigi Broggini Dipartimento di Scienza e Alta Tecnologia Universitàdegli Studi dell’Insubriavia Valleggio 922100 ComoItaly Sorbonne Université Facultédes Sciences et IngénierieCNRSInstitut Parisien de Chimie MoléculaireIPCM4 place Jussieu75005 ParisFrance DISFARM Sezione di Chimica Generale e Organica“A.Marchesini”Universitàdegli Studi di Milanovia Venezian 2120133 MilanoItaly

Treatment of O-allyl n-tosyl carbamates with aromatic compounds in the presence of Cu(OTf)_(2) or TMSOTf as promoters affords n-substituted 1-arylpropan-2-amines,1,2-diarylpropanes,1,1-diarylpropanes,or indanes,depending on the nature of the promoter and of the aryl substrates.A full mechanistic rational allowing appreciation of the outcome of these novel C–H based cascades is *** initial acid promoted decarboxylative/deamidative Friedel–Crafts allylation takes *** protonation of the allylated arene,evolution of the resulting cation may follow different paths depending on the nature of the arene partner and of the allyl moiety in the carbamate.

关键词： allyl moiety coupling

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Antimicrobial potential of aqueous coffee extracts against pathogens and Lactobacillus species:A food matrix application

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Food Bioscience 2022年第3期47卷 1142-1149页

作者： Luiza Andrea Canci marta de Toledo Benassi Cristiane Canan Daneysa Lahis Kalschne Eliane Colla Departamento de Alimentos Universidade Tecnológica Federal do ParanáAvenida Brasil4232Bairro Independência85884-000MedianeiraPRBrazil Centro de Ciências Agrárias Universidade Estadual de LondrinaRodovia Celso Garcia Cid PR 445 Km 38086057-970LondrinaParanáBrazil

Coffee is recognized by its bioactive potential,mainly antioxidant and antimicrobial *** aim of this study was to evaluate the antimicrobioal potential of aqueous extracts of Coffea arabica(AR)(roasted)and Coffea canephora[roasted(CR)and green(CG)]against patogens Salmonella Typhimurium and Escherichia coli,and probiotics Lactobacillus plantarum and Lactobacillus ***,the strain's sensitivity were tested by disk diffusion method,followed by the evaluation of growth kinetics(lag phase duration,maximum specific growth rate and logarithmic increase in the population)in culture media and in a food matrix(milk)by the adjustment of Gompertz predictive *** the step of strain sensivity tests,*** and *** were sensitive against all coffee extracts from 0.5%to 5.0%,while the Lactobacilli growth were not *** the growth kinetic tests in culture media,roasted coffees extracts reduced logarithmic increase in the population and maximum specific growth rate and improved lag phase for ***;for *** the growth was completely inhibited with 2.5%CR and 5.0%*** correlations between growth parameters with coffee bioactive contents suggested that melanoidins had the main antimicrobial *** the step of food matrix application(growth kinetics in milk),the addition of 5.0%CR reduced *** and *** multiplication,while the probiotics *** and *** where not inhibetd,suggesting a selective effect of coffee extracts as an antimicrobial agent,which may be of great interest for the food industry,especially for probiotic foods application.

关键词： Coffea arabica Coffea canephora Escherichia coli Green coffee Roasted coffee Salmonella Typhimurium

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Modularity and specialization in bat-fly interaction networks are remarkably consistent across patches within urbanized landscapes and spatial scales

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Current Zoology 2021年第4期67卷 403-410页

作者： gustavo Lima Urbieta gustavo Graciolli Jeferson Vizentin-Bugoimi Programa de Pós-Graduação em Ciencias Biológicas Departamento de Sistematica e EcologiaUniversidade Federal da ParaibaCidade Jardim Universitários/nCastelo BrancoJoão Pessoa 58051-900Brazil Programa de Pós-Graduação em Biologia Animal Laboratorio de SistematicaEcologia e Evoluqao(LSEE)Instituto de BiocienciasUniversidade Federal de Mato Grosso do Sul(UFMS)Campo Grande 79090-900Brazil Department of natural Resources and Environmental Sciences University of Illinois at Urbana-ChampaignUrbanaIL 61801USA

Patterns of specialization and the structure of interactions between bats and ectoparasitic flies have been studied mostly on non-urban environments and at local ***,how anthropogenic disturbances influence species interactions and network structure in this system remain poorly ***,we investigated patterns of interaction between Phyllostomidae bats and ectoparasitic Streblidae flies,and variations in network specialization and structure across Cerrado patches within urbanized landscapes in Brazil and between local and regional *** found high similarity in the richness and composition of bat and fly species across communities,associated with low turnover of interactions between *** high specialization of bat-streblid interactions resulted in little connected and modular networks,with the emergence of modules containing subsets of species that interact exclusively or primarily with each *** similarities in species and interaction composition and network structure across communities and scales suggest that bat-fly interactions within Cerrado patches are little affected by the degree of human modification in the surrounding *** remarkable consistency is likely promoted by specific behaviors,the tolerance of Phyllostomidae bats to surrounding urbanized landscapes as well as by the specificity of the streblid-bat interactions shaped over evolutionary time.

关键词： Chiroptera ectoparasites host-parasite network neotropics Streblidae urbanization

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Effect of Lepidium meyenii (maca) roots on spermatogenesis of male rats

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Asian Journal of Andrology 2001年第3期3卷 231-233页

作者： gustavo F.Gonzales Ana Ruiz Carla Gonzales León Villegas Amanda Cordova Department of Physiological Sciences Faculty of Sciences and Philosophy and Instituto de Investigaciones de la Altura. Universidad Peruana Cayetano Heredia. Postal Office 1843. Lima PeruDepartment of Physiological Sciences Faculty of Sciences and Philosophy and Instituto de Investigaciones de la Altura. Universidad Peruana Cayetano Heredia. Postal Office 1843. Lima PeruDepartment of Physiological Sciences Faculty of Sciences and Philosophy and Instituto de Investigaciones de la Altura. Universidad Peruana Cayetano Heredia. Postal Office 1843. Lima PeruDepartment of Physiological Sciences Faculty of Sciences and Philosophy and Instituto de Investigaciones de la Altura. Universidad Peruana Cayetano Heredia. Postal Office 1843. Lima PeruDepartment of Physiological Sciences Faculty of Sciences and Philosophy and Instituto de Investigaciones de la Altura. Universidad Peruana Cayetano Heredia. Postal Office 1843. Lima Peru

Aim: To determine the effect of oral administration of an aqueous extract from the roots of Lepidium meyenii (maca)on spermatogenesis in adult male rats. Methods; Male rats received an aqueous extract of the root (66.7 mg in onemL) twice a day for 14 consecutive days. Results: Treatment with Lepidium meyenii resulted in an increase in theweights of testis and epididymis but not the seminal vesicle weight. The length and frequency of stages IX-XIV seminif-erous tubules, where mitosis occurred, were increased and stages I-VI were reduced in rats treated with Lepidiummeyenii. Conclusion; The Lepidium meyenii root invigorates spermatogenesis in male rats by acting on its initialstages (IX-XIV). (Asian J Androl 2001 Sep; 3: 231 - 233)

关键词： spermatogenesis Lepidium meyenii maca transillumination

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Wheat genotypic variability in grain yield and carbon isotope discrimination under Mediterranean conditions assessed by spectral reflectance

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Journal of Integrative Plant Biology 2014年第5期56卷 470-479页

作者： gustavo A. Lobos Iván Matus Alejandra Rodriguez Sebastián Romero-Bravo José Luis Araus Alejandro del Pozo Plant Breeding and Phenomic Center Faculty of Agricultural Sciences University of Talca CRI-Quilamapu Agricultural Research Institute Plant Physiology Department Faculty of Biology University of Barcelona

A collection of 368 advanced lines and cultivars of spring wheat（Triticum aestivum L.） from Chile, Uruguay, and CIMMYT（Centro Internacional de Mejoramiento de Maíz y Trigo）, with good agronomic characteristics were evaluated under the Mediterranean conditions of central Chile. Three different water regimes were assayed： severe water stress（SWS, rain fed）, mild water stress（MWS; one irrigation around booting）, and full irrigation（FI; four irrigations： at tillering,flag leaf appearance, heading, and middle grain filling）. Traits evaluated were grain yield（GY）, agronomical yield components,days from sowing to heading, carbon isotope discrimination（△^13C） in kernels, and canopy spectral reflectance. Correlation analyses were performed for 70 spectral reflectance indices（SRI） and the other traits evaluated in the three trials. GY and △^13C were the traits best correlated with SRI, particularly when these indices were measured during grain filling. However,only GY could be predicted using a single regression, with Researchnormalized Difference Moisture Index（nDMI2： 2,200; 1,100）having the best fit to the data for the three trials. For △^13C, only individual regressions could be forecast under FI（r^2： 0.25–0.37）and MWS（r^2： 0.45–0.59） but not under SWS（r^2： 0.03–0.09）.nIR‐based SRI proved to be better predictors than those that combine visible and nIR wavelengths.

关键词： Breeding drought dry matter index normalized difference moisture index vegetative index water index

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Post-translational modifications of prostaglandin-endoperoxide synthase 2 in colorectal cancer:An update

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World Journal of Gastroenterology 2018年第48期24卷 5454-5461页

作者： Rafael I Jaén Patricia Prieto marta Casado Paloma Martín-Sanz Lisardo Boscá Department of Metabolism and Physiopathology of Inflammatory Diseases Instituto de Investigaciones Biomédicas Alberto Sols (CSIC-UAM) Department of Biomedicine Instituto de Biomedicina de Valencia (CSIC) Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares y Hepáticas y DigestivasISCⅢ Unidad Asociada IIBMULPGC Universidad de las Palmas de Gran Canaria (ULPGC)

The biosynthesis of prostanoids is involved in both physiological and pathological processes. The expression of prostaglandin-endoperoxide synthase 2(PTGS2; also known as COX-2) has been traditionally associated to the onset of several pathologies, from inflammation to cardiovascular, gastrointestinal and oncologic events. For this reason, the search of selective PTGS2 inhibitors has been a focus for therapeutic interventions. In addition to the classic non-steroidal anti-inflammatory drugs, selective and specific PTGS2 inhibitors, termed coxibs, have been generated and widely used. PTGS2 activity is less restrictive in terms of substrate specificity than the homeostatic counterpart PTGS1, and it accounts for the elevated prostanoid synthesis that accompanies several pathologies. The main regulation of PTGS2 occurs at the transcription level. In addition to this, the stability of the mRnA is finely regulated through the interaction with several cytoplasmic elements, ranging from specificmicroR nAs to proteins that control mR nA degradation. Moreover, the protein has been recognized to be the substrate for several post-translational modifications that affect both the enzyme activity and the targeting for degradation via proteasomal and non-proteasomal mechanisms. Among these modifications, phosphorylation, glycosylation and covalent modifications by reactive lipidic intermediates and by free radicals associated to the proinflammatory condition appear to be the main changes. Identification of these post-translational modifications is relevant to better understand the role of PTGS2 in several pathologies and to establish a correct analysis of the potential function of this protein in diseases progress. Finally, these modifications can be used as biomarkers to establish correlations with other parameters, including the immunomodulation dependent on molecular pathological epidemiology determinants, which may provide a better frame for potential therapeutic interventions.

关键词： Prostaglandins Prostaglandin-endoperoxide synthase 2 Post-translational modifications Glycosylation Colorectal cancer Inflammation

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Climate,female traits and population features as drivers of breeding timing in Mediterranean red deer populations

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Integrative Zoology 2017年第5期12卷 396-408页

作者： marta PELÁEZ Alfonso SAn MIGUEL Carlos RODRÍGUEZ-VIGAL Ramón PEREA Department of natural Systems and Resources Technical University of MadridMadridSpain national Parks Autonomous Agency MadridSpain Department of Biology Stanford UniversityStanfordCaliforniaUSA

Understanding the factors that lead to variation in the timing of breeding in widespread species such as red deer(Cervus elaphus)is crucial to predict possible responses of wild populations to different climate ***,we sought to further understand the causes of inter-annual variation in the reproduction timing of female deer in Mediterranean *** integrative approach was used to identify the relative importance of individual,population and climate traits in the date of conception of free-ranging deer,based on a dataset of 829 hinds culled during 12 *** found that a population trait,density,was the most important factor explaining the variation in conception dates,with greater densities causing later conception *** mass was the second in importance,with heavier females conceiving earlier than lighter *** equally important was the spring real bioclimatic index,a measure of plant productivity,causing later conception dates in the least productive springs(drier and hotter).Another climatic component,the end of summer drought,showed that the sooner the autumn arrives(greater rainfalls and cooler temperatures)the earlier the conception ***,age class was found to be a minor factor in determining conception *** older females(≥10 years old)conceived significantly later,suggesting reproductive *** study highlights not only the importance of population and individual traits but also the influence of climatic parameters on the deer reproductive cycle in Mediterranean environments,giving valuable insight into how reproductive phenology may respond to seasonality and global climate changes.

关键词： density-dependence mating short-day breeder summer drought ungulates

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Impact of systemic steroids combined with immunosuppressive treatment on glaucomatous features in patients with systemic lupus erythematosus

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International Journal of Ophthalmology(English edition) 2022年第1期15卷 71-76页

作者： marta P.Wiącek Danuta Bobrowska–Snarska Marek Brzosko Wojciech Lubiński Monika Modrzejewska First Department of Ophthalmology Pomeranian Medical UniversitySzczecin 70-111Poland Institute of Physics University of SzczecinSzczecin 70-451Poland Department of Rheumatology Internal Medicine and GeriatricsPomeranian Medical UniversitySzczecin 71-252Poland Second Department of Ophthalmology Pomeranian Medical UniversitySzczecin 70-111Poland

AIM:To evaluate the incidence of increased intraocular pressure(IOP)and glaucomatous changes in systemic lupus erythematosus(SLE)patients in comparison with systemic steroids and immunosuppressive ***:Sixty-two women with SLE were divided into two groups:treated(n=47,94 eyes)and not treated(n=15,30 eyes)with systemic glucocorticosteroids(GC;GC-free).Twenty-one individuals in GC group were treated with immunosuppressive agents(immunomodulating and biologic).The visual acuity and IOP with ocular pulsatile amplitude(OPA)measurements,as well as scanning laser polarimetry(GDx)with nerve fiber index(nFI)measurement,spectral domain optical coherence tomography(SD-OCT)of the optic disk with retinal nerve fiber layer(RnFL)analysis and the macular region with ganglion cell analysis(GCA)were ***:Mean IOP values in group with combined GC and immunosuppressive therapy was 15.8±2.56 mm Hg and was significantly lower than in individuals with exclusive GC treatment(17.63±4.38 mm Hg,P=0.043).Contrary,no dif ferences in mean IOP values between GC-free group and individuals treated with combined GC and immunosuppressive therapy were detected(P=0.563).Similarly,mean IOP in GC was 17.14±3.94 mm Hg and in GC-free patients was equal to 16.67±3.45 mm Hg(P=0.671).According to treatment regimen no statistical differences in optic disk SD-OCT for RnFL thickness,RnFL symmetry,cupping volume and the C/D ratio were ***,no statistical differences for the mean and minimal ganglion cell layer(GCL)thickness measured in macular SD-OCT or nFI in GDx were ***:Combined immunosuppressive and systemic GC therapy in SLE patients may lower the risk of iatrogenic ocular *** relationship between treatment regimen and glaucomatous damage of optic nerve fibers in analyzed groups with SLE is detected.

关键词： immunosuppression ocular hypertension steroid induced glaucoma steroid therapy systemic lupus erythematosus

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On the uncertainty of interwell connectivity estimations from the capacitance-resistance model

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Petroleum Science 2014年第2期11卷 265-271页

作者： gustavo A Moreno Larry W Lake YPF Macacha Güemes 515 YPF-Tecnología Baradero S/N (1925) Center for Petroleum and Geosystems Engineering University of Texas at Austin

The capacitance-resistance model （CRM） is an alternative to conventional reservoir simulation. CRM, a simplification of complex numerical models, uses production and injection rates to infer a reservoir description. There is no prior geologic model. The principal output of CRM fitting is the fraction of injected fluid （usually water） that is produced at a producer at steady-state. These fractions are interwell connectivities. Interwell connectivities are fundamental information needed to manage waterfloods in oil reservoirs. The data-driven CRM is a fast tool to estimate these parameters in mature fields and allows one to make full use of the dynamic data available. This paper considers the problem of setting an upper bound on the uncertainty of interwell connectivities for linear-constrained models. Using analytical bounds and numerical simulations, we derive a consistent upper limit on the uncertainty of interwell connections that can be used to quantify the information content of a given dataset.

关键词： Data-driven models capacitance-resistance model secondary recovery waterfloodoptimization interwell connectivities

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