检索结果-南通市图书馆

Detection of PRL-2 gene expression in hepatocellular carcinoma by real-time fluorescence quantitative PCR

在线全文

学校读者我要写书评

暂无评论

The Chinese-German Journal of Clinical Oncology 2009年第4期8卷 210-213页

作者： Chao Cheng Amos Ela Bella Ailin Guo guoyong wu Weikang wu Department of Thoracic Surgery The First Affiliated Hospital Sun Yat-sen University Guangzhou 510080 China Medical Research Center GuangDong Provincial People＇s Hospital Guangzhou 510080 China Department of Pathophysiology Sun Yat-sen University Guangzhou 510080 China

Objective:To quantitatively detect the expression level of PRL-2 in primary hepatocellular carcinoma using real-time fluorescence quantitative ***:Total RNA isolated from human HCC and liver tissue adjacent to the tumor was reversely transcribed into ***-time fluorescence quantitative PCR(Q-PCR) method was used to analyze the expres-sion level of PRL-2 ***:The Q-PCR method was performed successfully to precisely detect RNA ***-2 was expressed in all portal vein tumor thrombosis(PVTT) and HCC,but only in some paratumor *** highest expression level of PRL-2 gene was recorded in PVTT;meanwhile expression level of PRL-2 was higher than that in paratumor liver tis-sues and in HCC(P < 0.01),and it was higher in HCC than that in paratumor liver ***:The Q-PCR may be the most precise method to quantitatively detect RNA level and can be used in gene expression *** PRL-2 gene has higher expression in PVTT than that in HCC and in paratumor liver tissue cells,indicating that it plays an important role in the development and metastasis of the HCC.

关键词： carcinoma hepatocellular polymerase chain reaction PRL2 gene expression

POV-based molecular catalysts for highly efficient esterification of alcohols with aldehydes as acylating agents

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2024年第8期35卷 330-334页

作者： Zhikang wu guoyong Dai Qi Li Zheyu Wei Shi Ru Jianda Li Hongli Jia Dejin Zang Mirjana Colovic Yongge Wei Key Lab of Organic Optoelectronics&Molecular Engineering of Ministry of Education Department of ChemistryTsinghua UniversityBeijing 100084China State Key Laboratory of Natural and Biomimetic Drugs Peking UniversityBeijing 100191China Institute of Materia Medica Shandong First Medical University&Shandong Academy of Medical SciencesJi'nan 250117China University of Belgrade VincaInstitute of Nuclear Sciences-National Institute of the Republic of SerbiaDepartment of Physical ChemistryBelgrade 11351Serbia

The selective oxidative esterification of aldehydes with alcohols to the corresponding esters has been one of the hot spots in scientific research and industrial ***,the application of precious metal catalytic systems is limited by their complicated synthetic steps and high *** a highly efficient,green,recyclable selective synthesis method of esters catalyzed by polyoxovanadate(POV)-based molecu-lar catalysts has been developed in this *** results show that supramolecular interaction between POV and 1,3-dibenzylimidazolium bromide(Act_(2)Im)can efficiently convert alcohols and aldehydes to the corresponding esters in high yield under much milder *** insight is also provided based on the control experiments,single crystal X-ray diffraction and cyclic voltammetry studies.

关键词： POV Oxidation Supramolecular Esterification Catalyst

基于热重参数和蛋白质等指标的3种不同厂家螺蛳粉质量评价

在线全文

学校读者我要写书评

暂无评论

云南化工 2024年第6期51卷 34-38页

作者：李紫薇黎金铃翟怡芳黎晓莲梁秀任罗月萍余金凌吴国勇卢翠文周利兵广西科技师范学院广西来宾546199

为了评价螺蛳粉品质,选择不同厂家的螺蛳粉作为研究对象,测定了3种螺蛳粉的燃烧热、燃烧稳定性、蛋白质、脂肪、灰分、还原糖、微量元素。结果表明,3种螺蛳粉燃烧热大小顺序为:厂家6>厂家4>厂家5;燃烧稳定性排序为:厂家6>厂家5>厂家4;... 详细信息

为了评价螺蛳粉品质,选择不同厂家的螺蛳粉作为研究对象,测定了3种螺蛳粉的燃烧热、燃烧稳定性、蛋白质、脂肪、灰分、还原糖、微量元素。结果表明,3种螺蛳粉燃烧热大小顺序为:厂家6>厂家4>厂家5;燃烧稳定性排序为:厂家6>厂家5>厂家4;脂肪含量(%)大小顺序为:厂家6>厂家5>厂家4;灰分含量大小顺序为:厂家4>厂家5>厂家6;燃烧热、燃烧稳定性、蛋白质、还原糖、脂肪、灰分、微量元素含量多指标的顺序为:厂家4>厂家6>厂家5。研究建立了螺蛳粉的多指标分析及评价体系,为大规模开发螺蛳粉以及螺蛳粉分类研究提供有力的科学依据。

关键词：螺蛳粉热重分析 ICP-OES

Diagnosis of knee osteoarthritis by OCT and surface-enhanced Raman spectroscopy

在线全文

学校读者我要写书评

暂无评论

Journal of Innovative Optical Health Sciences 2022年第5期15卷 13-27页

作者： Ran An Huaimin Gu Zhouyi Guo Huiqing Zhong Huajiang Wei guoyong wu Yonghong He Shusen Xie Hongqin Yang School of Semiconductor Science and Technology South China Normal University Guangzhou 510631P.R.China Publications Department Optica 2010 Massachusetts Avenue NWWashingtonDC 20036 MOE Key Laboratory of Laser Life Science&Institute of Laser Life ScienceCollege of Biophotonics South China Normal University Guangzhou 510631P.R.China The First Affliated Hospital of Sun Yat-Sen University Guangzhou 510080 P.R.China Tsinghua University Shenzhen 518055P.R.China Fujian Normal University Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education of China Fuzhou 350007 P.R.China

In this paper,optical coherence tomography(OCT)and surface-enhanced Raman spectroscopy(SERS)were used to characterize normal knee joint(NKJ)tissue and knee osteoarthritis(KOA)tissue ex *** images show that there is a clear hierarchical structure in NKJ tissue,including surface layer,transitional layer,radiation layer and cartilage matrix calcification layer tissue structure,while the hierarchical structure of KOA tissue is not clear and unevenly distributed,and the pathological tissues at different stages also show significant di®*** shows that NKJ tissue and mild osteoarthritic knee cartilage(MiKOA)tissue have strong characteristic Raman peaks at 964,1073(1086),1271,1305,1442,1660 and 1763 cm^(-1).Compared with the Raman spectrum of NKJ tissue,the Raman characteristic peaks of MiKOA tissue have some shifts,moving from 1073 cm^(-1)to 1086 cm^(-1)and from 1542 cm^(-1)to 1442 cm^(-1).There is a characteristic Raman peak of 1271 cm^(-1)in MiKOA tissue,but not in NKJ *** with NKJ tissue,severely degenerated cartilage(SdKOA)tissues show some new SERS peaks at 1008,1245,1285,1311 and 1321 cm^(-1),which are not seen in SERS spectra of NKJ *** component analysis(PCA)was used to analyze the Raman spectra of 1245–1345 cm^(-1)*** results show that PCA can distinguish NKJ,MiKOA and SdKOA tissues and the accuracy is about 90%.These results indicate that OCT can clearly distinguish NKJ,MiKOA,moderate osteoarthritic knee cartilage(MoKOA)and SdKOA tissue,while SERS can provide further judgment *** results also prove that the contents of protein and polysaccharide in knee tissue are changed during the pathological process of knee tissue,which is the cause of pain caused by poor friction in knee joint during movement.

关键词： Optical coherence tomography surface-enhanced Raman spectroscopy knee osteoarthritis.

Effects of haemodilution with 0.9% normal saline and 7.5% hypertonic saline on the optical properties of blood during coagulation

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of haemodilution with 0.9% normal saline and 7.5% hy...

中国种植业碳补偿率区域差异、动态演进及收敛性分析

2015年中国生理学会运动生理学专业委员会会议暨“运动与心血管保护”学术研讨会

作者： Huajiang Wei Chengyi Liu guoyong wu Ying Liu Yuqing Zhang Zhenguo Zhu Laboratory of Laser Sports Medicine College of Physical Education and Sports ScienceSouth China Normal University Department of Surgery the First Affiliated HospitalSun Yat-Sen University

Background and Objective:Sodium is the major cation of the extracellular fluid and has a potent influence on fluid *** has been likened to a sponge that draws fluids into the extracellular space,including the plasma volume,to equalize gradients in *** are some extreme occupational or exercise-related conditions where sweat losses are great and Nalosses may exceed normal dietary *** can occur acutely such as in an ultra-endurance event or chronically as in hard manual work in the *** such cases,its wearable biometric diagnosis by using sensors such as bracelets,watches,skin patches,headbands,earphones,and clothing should be designed and *** optical/photonic sensors are a kind of ideal candidate of wearable biometric *** a primary investigation,the effects of dilution on blood coagulation with normal or hypertonic saline were studied using the optical coherence tomography technique in this *** Design/Materials and Methods:In this study,we investigated the effects of dilution with hypertonic(7.5%) and normal(0.9%) saline on the optical properties of blood during coagulation in vitro using optical coherence *** optical coherence tomography signal slope,1/e light penetration depth,and attenuation coefficient were obtained from the profiles of reflectance versus *** normal whole blood served as the control ***:After 1 h coagulation,the average optical coherence tomography signal slope totally decreased by 22.9%,22.7%,and 17.8%when 0.9% normal saline solution replaces 2%,11%,and20%of blood volume,*** total decreases were 23.7%,29.8%,and 33.6%,respectively,with 2%,11%,and 20%blood volume replacement by 7.5%hypertonic *** total decrease of the control group was 23%.The average coagulation time were(420 ± 16),(418± 16),and(358 ± 14) s,respectively,with blood volume replacement of 2%,11%,and 20%by0.9%normal *** 2%,11%,and 20%blood volume replacement with 7.5%hy

关键词： blood sodium coagulation optical coherence tomography

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

中国生态农业学报（中英文） 2021年第10期29卷 1774-1785页

作者：伍国勇陈莹孙小钧贵州大学经济学院(西部中心) 贵阳550025 南京农业大学经济管理学院南京210095 贵州基层社会治理创新高端智库贵阳550025

农业碳排放阻碍绿色农业转型,探索种植业碳补偿率区域差异、动态演进及收敛性,可为低碳农业良性发展提供有益指导。本文同时考虑碳源和碳汇,测算2002—2018年中国31省、市、自治区种植业碳补偿率,采用Dagum基尼系数分解法考察地区差异,... 详细信息

农业碳排放阻碍绿色农业转型,探索种植业碳补偿率区域差异、动态演进及收敛性,可为低碳农业良性发展提供有益指导。本文同时考虑碳源和碳汇,测算2002—2018年中国31省、市、自治区种植业碳补偿率,采用Dagum基尼系数分解法考察地区差异,采用非参数估计中的核密度估计动态演进过程,借助σ收敛、绝对β收敛及条件β收敛检视收敛性特征。结果表明:1)种植业碳补偿率整体相对差异扩大趋势明显。东部地区相对差异扩大,中部地区和西部地区变化较小;东—西部、东—中部地区之间增加,中—西部地区之间减小;地区间差距是造成种植业碳补偿率差异的主要原因。2)中国种植业碳补偿率整体呈逐年增大的变动态势,碳补偿率高值省份有所增多,省域种植业碳补偿率差异有先减后增的趋势。东部各省种植业碳补偿率在逐渐上升,绝对差距有所减少,从两极分化演变为单极化;中部各省种植业碳补偿率在逐渐上升,绝对差距有所减小;西部各省种植业碳补偿率变化较为稳定。3)全国及东、西部地区的种植业碳补偿率不存在σ收敛,而中部地区不甚明显;全国、东、中及西部地区绝对和条件β收敛均显著。本文的结论强调,中国种植业碳补偿率的区域异质性凸显,其时序变化趋势总体上升;省域间的“追赶效应”显现,地区间碳补偿率增长的趋同态势明显。因此,合理制定区域农业绿色发展策略,积极发挥区域减排潜力是提高种植业碳补偿率的关键。

关键词：种植业碳补偿率区域差异动态演进收敛性

在线全文

学校读者我要写书评

暂无评论

中国粮食生产效率空间溢出网络及提升路径

地理学报 2022年第4期77卷 996-1008页

作者：张启楠张凡凡麦强伍国勇哈尔滨工业大学经济与管理学院哈尔滨150001 贵州大学中国喀斯特地区乡村振兴研究院贵阳550025 贵州基层社会治理创新高端智库贵阳550025

现有研究普遍关注粮食生产单元自身资源禀赋以及功能属性,对于经济主体相互作用所产生的外部性研究十分有限。为此,本文在空间引力模型的基础上着重分析网络外部性对粮食生产效率的影响,并以此为逻辑起点进一步探讨网络提升路径以及各... 详细信息

现有研究普遍关注粮食生产单元自身资源禀赋以及功能属性,对于经济主体相互作用所产生的外部性研究十分有限。为此,本文在空间引力模型的基础上着重分析网络外部性对粮食生产效率的影响,并以此为逻辑起点进一步探讨网络提升路径以及各空间单元在整体网络中的综合定位与潜力。结果表明,中国粮食生产网络空间溢出效应显著,对粮食生产效率具有明显的促进作用,其实质是规模经营思想在空间维度的扩展,符合近带动远辐射规律,且交通基础设施的互通互联与人口流动是实现网络空间外溢的主要路径。同时,整体网络中共存在4种潜在节点,包括核心控制型、局域核心型、潜力型以及边缘型。未来应充分发挥核心控制型节点的辐射带动作用,加强东西轴带的空间交互影响,实现农业资源技术的跨地域流动与互补。

关键词：粮食生产效率空间网络外部性空间计量模型二次指派程序潜在网络节点中国

在线全文

学校读者我要写书评

暂无评论

基于四阶段DEA的安徽省耕地利用效率分析

水土保持通报 2018年第4期38卷 288-294页

作者：陈晨伍国勇贵州大学管理学院贵州贵阳550025 贵州大学中国喀斯特地区乡村振兴研究院贵州贵阳550025

[目的]运用更为准确有效的手段评价安徽省耕地利用效率,为安徽省耕地的集约高效利用提供参考依据。[方法]利用四阶段DEA方法测算2016年安徽省16市的耕地效率值。[结果](1)安徽省16个市综合效率均值为0.743,表明安徽省耕地利用效率整体... 详细信息

[目的]运用更为准确有效的手段评价安徽省耕地利用效率,为安徽省耕地的集约高效利用提供参考依据。[方法]利用四阶段DEA方法测算2016年安徽省16市的耕地效率值。[结果](1)安徽省16个市综合效率均值为0.743,表明安徽省耕地利用效率整体水平不高,纯技术效率和规模效率均有待提升。合肥市、亳州市、宿州市、六安市4市耕地的投入与产出比已达最优,蚌埠市等6市仅技术有效,其他6市不存在技术或规模有效;除阜阳市和最优4市外,其余11市耕地规模化经营尚具有一定潜力。(2)提高耕地复种指数和提高农民人均可支配收入均能够缓解各类投入资源的过剩程度,改善农田水利条件有利于提高耕地面积的利用率,增加劳均播种面积也会缓解劳动力的过度供给。[结论]适当提高复种指数,改善农田水利条件、促进农民非农就业,以区域发展带动农业发展是实现安徽省耕地高效集约利用的可行途径。

关键词：四阶段DEA 耕地资源利用效率安徽省

在线全文

学校读者我要写书评

暂无评论

不等精度直剪试验数据处理

广东水利水电 2017年第9期 34-35,39页

作者：吴国永广东省水利水电科学研究院广东广州510635

由于土质差异等原因,土的直剪试验往往是不等精度的,将其作等精度处理,会使土的抗剪强度指标引入不必要的不确定度。该文导出不等精度试验直线参数的正规方程,给出不等精度直剪试验数据处理实例,通过比较不同数据处理方法计算结果的不... 详细信息

由于土质差异等原因,土的直剪试验往往是不等精度的,将其作等精度处理,会使土的抗剪强度指标引入不必要的不确定度。该文导出不等精度试验直线参数的正规方程,给出不等精度直剪试验数据处理实例,通过比较不同数据处理方法计算结果的不确定度,了解这些方法的可靠程度。

关键词：直剪试验不等精度抗剪强度指标数据处理不确定度