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Single PdO loaded on boron nanosheet for methane oxidation:A DFT study

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Progress in Natural Science:Materials International 2019年第3期29卷 367-369页

作者： Ani Dong guowang xu Zhongxu Dai Aimin Yu Siyao Qiu Chenghua Sun Department of Computer and Information Science City College of Dongguan University of Technology Department of Chemistry and Biotechnology and Centre for Translational AtomaterialsFaculty of ScienceEngineering and TechnologySwinburne University of Technology Science & Technology Innovation Institute Dongguan University of Technology College of Materials and Chemical Engineering and Hubei Key Laboratory of Natural Products Research and DevelopmentChina Three Gorges University

The preparation of methanol （CH;OH） from methane （CH;） oxidation has been viewed as a promising technique to obtain liquid fuel from natural *** was demonstrated that oxygen was adsorbed on single palladium （PdO） as an excellent catalyst for the CH;-to-CH;OPH conversion under the scheme of density functional *** catalyst PdO loaded on boron sheet shows excellent stability and CH;OH *** calculations reveal that the boron substrate remarkably tunes the state of palladium.

关键词： CH Single PdO loaded on boron nanosheet for methane oxidation:A DFT study DFT

Establishment and drug sensitivity evaluation of murine ascites hepatocarcinoma cell line with high lymphatic metastatic potential (Hca-P/L_(6))

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Frontiers of Medicine 2009年第2期3卷 119-129页

作者： Hongying ZHANG Jianwu TANG Wenting ZHU Chunxiu HU guowang xu Department of Pathology&Forensic Medicine Dalian Medical UniversityDalian 116044China National Chromatographic R&A Center Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China

In order to provide a sensitive cell line model for investigating the mechanisms underlying the lymphatic metastasis of tumors and the effect of medicine against cells,a new murine ascites hepatocarcinoma cell line with high lymphatic metastatic potential(Hca-P/L_(6))was established and the effect of curcumin on biological behavior of Hca-P/L_(6) was *** ascites hepatocarcinoma cell strain with low lymphatic metastatic potential(Hca-P)was subcutaneously inoculated into the medioventral line of a mouse 615 and theﬁrst generation of metastatic tumor cells of inguinal lymph node(Hca-P/L_(1))was ***,Hca-P/L_(1) was screened by the route of mouse foot pad subcutaneously!lymph node!scale-up culture in vitro!mouse foot pad subcuta-neously forﬁve times *** sensitivity of two murine ascites hepatocarcinoma cell lines(Hca-P and Hca-P/L_(6))and two anchorage-dependent human hepato-carcinoma cell lines(SMC7721 and HepG_(2))to curcumin were studied by use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay after these cells had been pretreated by curcumin at the concentration of 15–240μmol/L for 48 *** pretreatment by curcumin at the maximum non-cytotoxic dose of 15μmol/L in vitro,the effect of curcumin against cell proliferation of Hca-P and Hca-P/L6 was observed by inverted micro-scope,cell growth curve and cell population doubling time;the effects of curcumin on cell cycles of Hca-P/L6 and Hca-P were studied byﬂow cytometry(FCM).The results showed Hca-P/L_(6) spreading to the lymph nodes at multiple sites in mice was screened from *** lymph node metastatic rate was 100%.Curcumin had signiﬁcant growth inhibiting effect on both murine ascites and human hepatocarcinoma cell lines in a dose-dependent manner(P<0.05).At concentrations of 30–120μmol/L,curcu-min had more inhibition on murine ascites hepatocarci-noma cell lines than on human anchorage-dependent hepatocarcinoma cell *** concentrations of 60–240μmo

关键词： murine ascites hepatocarcinoma cell line metastasis curcumin drug sensitivity

A metabolome atlas of mouse brain on the global metabolic signature dynamics following short-term fasting

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Signal Transduction and Targeted Therapy 2023年第10期8卷 4838-4849页

作者： Yaping Shao Zhenfa Fu Yanfeng Wang Zhaofei Yang Yushan Lin Song Li Cheng Cheng Min Wei Zheyi Liu guowang xu Weidong Le Liaoning Provincial Key Laboratory for Research on the Pathogenic Mechanisms of Neurological Diseases The First Affliated HospitalDalian Medical University193 Lianhe Road116021 DalianChina CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of Sciences457 Zhongshan Road116023 DalianChina Institute of Neurology Sichuan Academy of Medical Science-Sichuan Provincial HospitalMedical School of UESTC611731 ChengduChina

Calorie restriction(CR)or a fasting regimen is considered one of the most potent non-pharmacological interventions to prevent chronic metabolic disorders,ameliorate autoimmune diseases,and attenuate *** efforts,the mechanisms by which CR improves health,particularly brain health,are still not fully *** homeostasis is vital for brain function,and a detailed metabolome atlas of the brain is essential for understanding the networks connecting different brain regions.

关键词： fasting homeostasis diseases

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Editorial

Journal of Analysis and Testing 2020年第3期4卷 139-139页

作者： guowang xu CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China

Warmly invited by the JOAT,as a guest editor,I organized this special issue on metabolomics:state of art in method development and *** manuscripts are included in this issue including 5 reviews and 4 research *** is a science to study the endogenous metabolites in a biological *** has been used to discover new potential biomarkers for disease diagnosis and typing,and help pharmaceutical R.&D.,and it is also playing an important role in plant and microbe *** perform metabolomics,analytical methods are very *** this issue,focusing on challenges of liquid chromatography-mass spectrometry(LC-MS)in analysis of hydrophilic metabolomics analysis,Wang et *** the nonderivatization and derivatization strategies,with the intention of providing multiple choices for analysis of hydrophilic metabolites.

关键词： diagnosis endogenous editor

Glycolytic potential enhanced by blockade of pyruvate influx into mitochondria sensitizes prostate cancer to detection and radiotherapy

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Cancer Biology & Medicine 2022年第9期19卷 1315-1333页

作者： Huan xu Junyi Chen Zhi Cao Xi Chen Caihong Huang Jin Ji Yalong xu Junfeng Jiang Yue Wang guowang xu Lina Zhou Jingyi He xuedong Wei Jason Boyang Wu Zhong Wang Shancheng Ren Fubo Wang Department of Urology Shanghai Changhai HospitalSecond Military Medical UniversityShanghai 200433China Department of Urology Shanghai Ninth People’s HospitalShanghai 200011China Department of Urology the Second Affiliated Hospital of Fujian Medical UniversityQuanzhou 362000China Department of Urology No.971 Hospital of the People’s Liberation Army NavyQingdao 266000China Center for Genomic and Personalized Medicine Guangxi Key Laboratory for Genomic and Personalized MedicineGuangxi Collaborative Innovation Center for Genomic and Personalized MedicineGuangxi Medical UniversityNanning 530021China Research Center of Developmental Biology Second Military Medical UniversityShanghai 200433China Dalian Institute of Chemical Physics Chinese Academy of SciencesDalian 116023China Department of Urology the First Affiliated Hospital of Soochow UniversitySuzhou 215006China Department of Pharmaceutical Sciences College of Pharmacy and Pharmaceutical SciencesWashington State UniversitySpokaneWA 99202USA

Objective:This study aimed to evaluate the effects of mitochondrial pyruvate carrier(MPC)blockade on the sensitivity of detection and radiotherapy of prostate cancer(PCa).Methods:We investigated glycolysis reprogramming and MPC changes in patients with PCa by using metabolic profiling,RNASeq,and tissue *** blockade of pyruvate influx into mitochondria was observed in cellular studies to detect its different effects on prostate carcinoma cells and benign prostate *** mouse models were injected with an MPC inhibitor to evaluate the sensitivity of 18F-fluorodeoxyglucose positron emission tomography with computed tomography and radiotherapy of ***,the molecular mechanism of this different effect of transient blockage towards benign prostate cells and prostate cancer cells was studied in ***:MPC was elevated in PCa tissue compared with benign prostate tissue,but decreased during cancer *** transient blockade increased PCa cell proliferation while decreasing benign prostate cell proliferation,thus increasing the sensitivity of PCa cells to 18F-PET/CT(SUVavg,P=0.016;SUVmax,P=0.03)and radiotherapy(P<0.01).This differential effect of MPC on PCa and benign prostate cells was dependent on regulation by a VDAC1-MPC-mitochondrial homeostasis-glycolysis ***:Blockade of pyruvate influx into mitochondria increased glycolysis levels in PCa but not in non-carcinoma prostate *** transient blockage sensitized PCa to both detection and radiotherapy,thus indicating that glycolytic potential is a novel mechanism underlying PCa *** change in the mitochondrial pyruvate influx caused by transient MPC blockade provides a critical target for PCa diagnosis and treatment.

关键词： Glycolytic potential prostate cancer mitochondrial pyruvate carrier(MPC) mitochondria pyruvate influx diagnosis radiotherapy

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The Role of Expression of Mismatch Repair Proteins hMSH2 and hMLH1 in Gastric Carcinogenesis and Its Clinical Significance

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Chinese Journal of Clinical Oncology 2007年第5期4卷 351-354页

作者： Mei Li Lina Liu Zhaohui Wang Lihong Wang Zhimin Liu guowang xu Shen Lv Experimental Center the Second Hospitalof Dalian Medical University Dalian 116027China Dalian Chemistry ＆ Physics Research Institute Chinese Academy of Science Dalian116027 China

OBJECTIVE To investigate the expression of the mismatch repair proteins hMSH2 and hMLH1, and to examine the clinical significance of the intracellular expression site （ICES） in gastric carcinogenesis. METHODS Specimens from 172 cases of gastric cancer, 151 tissues from paraneoplastic gastric mucosa and 34 from noncancerous gastric mucosa were collected in Dalain, China. An immunohistochemical method was used to determine the expression of the hMSH2, hMLH1 proteins and their ICES in the gastric mucosas. RESULTS The rate of hMSH2 expression in gastric cancers, paraneoplastic gastric mucosas and noncancerous gastric mucosas were respectively 69.8%, 49.7% and 32.4%. The rate was significantly higher in gastric cancer compared to the latter two groups （P=0.000）, but there was no obvious difference in the expression between the two latter groups （P=0.067）. The hMLH1 protein expression rates were respectively 73.3%, 57.6% and 41.2% in the above three groups. The expression was significantly higher in the gastric cancer group compared to the two latter groups （P=0.000）, while there was no significant difference between the latter groups （P=0.082）. There was no obvious correlation between the hMSH2 and hMLH1 protein expression rates and related factors, such as gender, age and differentiated level of gastric cancer etc. The cell-nuclear expression of the hMSH2 protein was respectively 70.0%, 58.7% and 36.4% in the gastric cancer, paraneoplastic gastric mucosa and noncancerous gastric mucosa groups. The cytoplasmic expression rates were 30.0%, 41.3% and 63.6% in the three groups. The cell-nuclear expression rate of the hMSH2 protein gradually decreased in the gastric mucosas in the following order： cancer, paraneoplastic and noncancerous but cytoplasmic expression only increased slightly in these groups （r=0.161, P=0.020）. There was no significant difference in the ICES of the hMLH1 protein among the three different gastric mucosas （P=0.659）. CONCLUSION Simultaneous det

关键词： hMSH2 hMLH1 gastric cancer immunohistochemistry

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The Effect of Helicobacter pylori Infection on hMSH2 and P53 Proteins in Gastric Carcinogenesis

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Chinese Journal of Clinical Oncology 2005年第5期2卷 791-795页

作者： Kai Fan Jianmei Ma Zhaohui Wang Min Liu Mei Li Shen Lue Lina Liu guowang xu Central Laboratory the Second Affiliated Hospital of Dalian Medical UniversityDalian 116027 China. Department of Anatomy DaLian Medical University Dalian 116027 China. Department of Gastrointestine the First Affiliated Hospital DaLian Medical University Dalian 116011 China. Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian 116023 China.

OBJECTIVE To investigate the effect of Helicobacter pylori （H pylon） infection on expression of hMSH2 and P53 and its possible carcinogenic mechanism. METHODS H pylori infection was detected using a rapid urease test and haematoxylin and eosin （H＆E） staining. The hMSH2 and P53 proteins in gastric cancer （GC） tissue, its adjacent mucosa, gastritic mucosa and normal gastric mucosa were detected by the immunohistochemical SP method. RESULTS （1） The positive rate of hMSH2 expression in GC tissue （62.7%）was higher than those in non-cancer tissues （P〈0.001）; the positive rate of hMSH2 expression in poorly differentiated adenocarcinoma （76.4%） was higher than that in other carcinomas （P〈0.05）. The positive rate of P53 expression in GC tissue （51.9%） was higher than that in noncancer tissues （P〈0.001）; the positive rate of P53 expression in well-differentiated adenocarcinoma （32.6 %） was lower than that in other carcinomas （P〈0.01）. （2） The positive rate of hMSH2 expression in GC tissue with H pylori infection was lower than that without the infection （52.8% vs. 74.5%; P〈0.05）. The positive rate of P53 expression in GC tissue with H pylori infection was higher than that without the infection （61.4% vs. 40.6%; P〈0.05）.（3） The expression of hMSH2 and P53 in GC tissue correlated positively （r=0.457, P〈0.01）. CONCLUSION High expression of hMSH2 and P53 as well as their interaction may be involved in gastric carcinogenesis; H pylori infection affecting expression of hMSH2 and P53 may be one of its carcinogenic mechanisms.

关键词： helicobacter pylori gastric cancer hMSH2 P53 immunohistochemistry.

Changes of hMSH2 and hMLH1 Expression in Nasopharyngeal Carcinoma Cells after X-Radiation

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Chinese Journal of Clinical Oncology 2005年第4期2卷 700-705页

作者： Kai Fan Hui Wang Zhihong Yu Jing Fu Yan Wang Min Liu Mei Li Shen Lü guowang xu Central Laboratory the Second Affiliated Hospital of Dalian Medical University Dalian 116027 China Department of Radiotherapy the Second Affiliated Hospital of Dalian Medical University Dalian 116027 China Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian 116023 China

OBJECTIVE To study the effect of X-radiation on expression of hMSH2 and hMLH1 in nasopharyngeal carcinoma （NPC） CNE-1 cells, and explore the mechanism of DNA mismatch repair after radiation. METHODS The cells were divided into experimental and control groups. Experimental cells were exposed to 10 Gy radiation administered as 2 Gy per fraction. Control cells were not radiated. The distribution of cells in the cell cycle was analyzed using flow cytometry. The expression of hMSH2 and hMLH1 was examined by RT-PCR and Western blots. RESULTS The expression of hMSH2 mRNA in experimental cells was significantly greater compared to control cells at 0-3rd weeks and decreased at the 4th week following radiation （P〈0.01）. The expression of hMSH2 protein in experimental cells was up-regulated and significantly greater compared to control cells at the 2nd-4th weeks after radiation （P〈0.01）. There were no significant differences in hMLH1 mRNA and protein expression between experimental and control cells （P 〉0.05）. CONCLUSION Radiation induces hMSH2 expression; hMSH2 has a role in the process of DNA repair, which maybe responsible for reduction of radiosensitivity after radiation.

关键词： X-radiation nasopharyngeal carcinoma hMSH2 hMLH1

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毛细管电泳核酸分析用于重大疾病诊断的研究

分析化学 2000年第4期28卷 507-512页

作者：梅素容许国旺吴采樱 116011 430072 中国科学院大连化学物理研究所!国家色谱研究分析中心武汉大学化学学院武汉大学化学学院!武汉

对毛细管电泳核酸（包括基因和核苷）分析在重大疾病诊断中的应用作了较为详细的论述，同时概括了相关的毛细管电泳方法和聚合酶链反应，并对今后的工作作了展望。

关键词：毛细管电泳核酸基因核苷聚合酶链反应病因诊断评述