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Chinese Science Bulletin 2001年第10期46卷 806-811页

作者： YANG Yi liu guoqin YAN Longfei College of Biological Sciences China Agricultural UniversityBeijing 100094China Laboratory of Plant Physiology and Biochemistry Ministry of AgricultureChina

The fusion gene of actin (cDNA of Chlamydo-monas reinhardtii) and green fluorescence protein (gfp) had been constructed into two expression vectors which could be expressed in E. coli and tobacco suspension cells BY2. The correct expression was observed in E. coli and BY2 with a fluorescence microscopy. The fusion protein, which took part in the membrane skeleton, was mainly located peripherally along the membrane, specially the fusion protein was distributed around nucleus and cell plate, while the fusion protein also forms F-actin in the cell. The fusion protein was purified from B121plus by ammonium sulfate fractionation, ion exchange chromatography and hydrophobic interaction chromatography. The purified production could polymerize into F-actin when the actin polymerizing buffer was *** was demonstrated that the characteristics and function of actin in Chlamydomonas was similar with those of animals and higher plants.

关键词： actin gfp prokaryotic expression eukaryotic expression.

From pollen actin to crop male sterility

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Chinese Science Bulletin 2000年第9期45卷 784-788页

作者： YAN Longfei liu guoqin XIAO Xingguo Laboratory of Plant Physiology and Biochomistry Ministry of AgriultureCollege of Biological SciencesChina Agricultural UniversityBeijing 100094China

Actin plays an important role in the life activity of animal and plant cells. Pollen cells have plenty of actin whose structure and characteristics are very similar to the animal actin. The nucleotide sequence and amino acid sequence of plant actin gene are very similar to those of the animal gene. The content of pollen actin from male sterile plants is much more lower than that from its maintainer plants. The expression of actin gene is organ-specific during the plant development. The expression quantity of actin gene in pollen is much more higher than those from root, stem and leaf. The expression plasmid of the anti-sense actin gene was constructed, transferred to the protoplasts of wheat and tomato to inhibit the expression of actin gene in pollen and thus the male sterile plants of wheat and tomato were obtained. The actin in pollens from the transgenic plants was reduced significantly, whereas the pistil was not affected. This study might pave a new way to breeding male sterile lines for the

关键词： actin pollen male sterility.

Purification and characterization of myosin from wheat mitochondria

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Chinese Science Bulletin 2002年第4期47卷 315-318页

作者： ZHAO Heping liu Aixiao liu guoqin YEN Lungfei College of Biological Sciences China Agricultural UniversityKey Laboratory of Plant Physiology and BiochemistryMinistry of AgricultureBeijing 100094China Institute of Cell of Biology College of Life SciencesBeijing Normal UniversityBeijing 100875China

Myosin was purified from wheat mitochondria using DE-52 anion exchange chromatography and Sephacryl S-300 gel filtration. The molecular weight of its heavy chain is about 210 ku, similar to that of muscle myosin II (205 ku), and it could be recognized by the polyclonal antibodies against human skeletal muscle myosin II. The ATPase ac-tivity of the mitochondrial myosin stimulated by F-actin from chicken muscle is 202.5 nmoles Pi/min·mg. The mito-chondrial myosin could be activated by Ca2+ and was not inhibited by Ca2+ at high concentration. The results demon-strate that the myosin of wheat mitochondria shares some similarities with the skeletal muscle myosin II.

关键词： myosin mitochondria purification ATPase.

Post-transcriptional gene silencing signal could move rapidly and bidirectionally in grafted Arabidopsis thaliana

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Chinese Science Bulletin 2006年第3期51卷 313-319页

作者： LI Ming JIANG Shiling WANG Youqun liu guoqin State Key Laboratory of Plant Physiology and Biochemistry College ofBiological Sciences China Agricultural University Beijing 100094China

RNA interference (RNAi), one of the newly found ways for post-transcriptional gene si- lencing, has been widely used to investigate gene functions through transgenic methods for introducing an RNA silencing signal into plants. In the present study, we constructed a dexamethazone (DEX)-inducible RNAi binary vector harboring a specific sequence fragment (168-bp) homologous to KatB and KatC, two kinesin isoform genes of Arabidopsis, which were proved to result in the post-transcriptional gene si- lencing of KatB and KatC in DEX-induced transgenic plants. RT-PCR and Northern blot analysis on trans- genic homozygous Arabidopsis (termed as RNAi-type plants) showed that DEX inducement causes KatB and KatC mRNA degradation. With a simplified method, Arabidopsis grafting was effectively per- formed between RNAi-type and wild-type lines. The target gene mRNA levels were tested based on semi-quantitative RT-PCR. Our results demonstrateed that DEX-induced gene silencing signals could result in a reduction in KatB and KatC mRNA in the wild-type rootstocks or scions, indicating that silencing signals of RNAi could be transmitted bidirectionally across the graft junction whether RNAi-plants were scions or stocks. In contrast to the previously reported results on grafted tobacco, the transmission of post- tran- scriptional gene silencing signals caused by RNAi in grafted Arabidopsis is more effective than that in to- bacco.

关键词：阿布属嫁接 RNA干预基因抑制信号传导烟草

Ni2P Nanosheets:A High Catalytic Activity Platform for Electrochemical Detection of Acetaminophen

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Chinese Journal of Chemistry 2021年第7期39卷 1849-1854页

作者： Ming Wei Wenbo Lu guoqin liu Yimin Jiang Xuebo liu Liwei Bai Xiaowei Cao Jianfeng Jia Haishun Wu Key Laboratory of Magnetic Molecules and Magnetic Information Materials(Ministry of Education) School of Chemistry and Material ScienceShanxi Normal UniversityLinfenShanxi041004 China Kangda College of Nanjing Medical University LianyungangJiangsu222000 China Institute of Translational Medicine Medical CollegeYangzhou UniversityYangzhouJiangsu225001 China

Main observation and conclusion Accurate determination of acetaminophen concentration is essential for studying the metabolic status of acetaminophen in clinical *** this study,nickel phosphide was used for electroche... 详细信息

关键词： Acetaminophen Nickel phosphide Electrochemistry Sensors Nanotechnology

Molecular cloning, expression and biochemical property analysis of AtKP1, a kinesin gene from Arabidopsis thaliana

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Chinese Science Bulletin 2007年第10期52卷 1338-1346页

作者： LI XuYan WANG HaiQing XU Tao CAO QinHong REN DongTao liu guoqin State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing 100094 China Northwest Plateau Institute of Biology the Chinese Academy of Sciences Xining 810001 China

Kinesins are common in a variety of eukaryotic cells with diverse functions. A cDNA encoding a member of the Kinesin-14B subfamily is obtained using 3′-RACE technology and named AtKP1 (for Arabidopsis kinesin protein 1). This cDNA has a maximum open reading frame of 3.3 kb encoding a polypeptide of 1087 aa. Protein domain analysis shows that AtKP1 contains the motor domain and the calponin homology domain in the central and amino-terminal regions, respectively. The carboxyl-terminal region with 202 aa residues is diverse from other known kinesins. Northern blot analysis shows that AtKP1 is widely expressed at a higher level in seedlings than in mature plants. 2808 bp of the AtKP1 promoter region is cloned and fused to GUS. GUS expression driven by the AtKP1 promoter region shows that AtKP1 is mainly expressed in vasculature of young organs and young leaf trichomes, indicating that AtKP1 may participate in the differentiation or development of Arabidopsis thaliana vascular bundles and trichomes. A truncated AtKP1 protein containing the putative motor domain is expressed in E. coli and affinity-purified. In vitro characterizations indicate that the polypeptide has nucleotide-dependent microtubule-binding ability and microtubule-stimulated ATPase activity.

关键词：拟南芥驱动蛋白 AtKP1 分子克隆基因表达生物化学性质

Agrobacterium tumefaciens-mediated transformation of modern rose(Rosa hybrida)using leaf-derived embryogenic callus

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Horticultural Plant Journal 2021年第4期7卷 359-366页

作者： guoqin liu Yuan Yuan Hui Jiang Ying Bao Guogui Ning Liangjun Zhao Xiaofeng Zhou Hougao Zhou Junping Gao Nan Ma State Key Laboratory of Agrobiotechnology Beijing Key Laboratory of Development and Quality Control of Ornamental CropsDepartment of Ornamental HorticultureCollege of HorticultureChina Agricultural UniversityBeijing 100193China Faculty of Life Science Tangshan Normal UniversityTangshanHebei 063000China Key Laboratory of Horticultural Plant Biology College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070China College Horticulture&Landscape Architecture Zhongkai University of Agriculture and EngineeringGuangzhou 510225China

Rose(Rosa hybrida)is widely used for cut flowers and as garden *** and efficient transformation system is required for functional genomics of ***,we established an efficient transformation method for rose using Agrobacterium tumefaciens-mediated transformation of embryogenic *** rose leaves were used as explants to induce somatic embryos,which were subjected to transformation with *** strain GV3101 using Green Fluorescence Protein(GFP)as a marker *** took about 8 months to generate transgenic shoots from embryogenic ***,RT-PCR,Southern and Western blotting,as well as stereoscopic fluorescence microscopy analysis demonstrated that GFP transgenes integrated stably into the rose *** to our data,a transformation efficiency of up to 6%can be achieved by following this optimized protocol.

关键词： Rose Rosa hybrida Leaf Somatic embryos Agrobacterium tumefaciens Genetic transformation

Tribological and mechanical behaviours of resin-based friction materials based on microcrack filling

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Biosurface and Biotribology 2023年第1期9卷 1-8页

作者： Lekai Li Guixiong Gao Jin Tong Jian Zhuang Wei Song Yunhai Ma guoqin liu Feipeng Cao Shengwang Yuan Qifeng Zhang Key Laboratory of Bionic Engineering(Ministry of Education) College of Biological and Agricultural EngineeringJilin UniversityChangchunChina

To enhance the friction performance of resin-based friction materials,five types of specimens with different polymer ether ketone(PEEK)contents were fabricated and their physiomechanical behaviours were tested and,their tribological properties were investigated using a JF150F-II constant-speed *** was found that the addition of PEEK had a positive influence on the properties of the friction materials,and sample FM-3(the shorthand of‘Friction Materials-3’,containing 2 wt%PEEK)exhibited improved friction performance with a fade ratio and recovery ratio of 8.6%and 101.1%*** all samples,FM-4(the shorthand of‘Friction Materials-4’,con-taining 3 wt%PEEK)had the lowest specific wear rate with a value of 0.622×10^(−7)cm^(3)(N⋅m)^(−1)at 350℃.The PEEK can fill the microcracks in the composite at a high tem-perature and can also cover the hard abrasive particles to prevent them from directly damaging the *** findings from this study afford a foundation for studies to further improve the properties of resin-based friction materials.

关键词： fade ratios friction materials PEEK recovery ratios specific wear rate

溶胶-凝胶法辅助碳热还原氮化反应制备CeN粉末

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稀有金属材料与工程 2023年第11期52卷 3730-3735页

作者：刘国琴连利仙刘颖四川大学材料科学与工程学院四川成都610065

以柠檬酸(CA)为螯合剂,即以有机物为碳源,通过溶胶-凝胶法辅助碳热还原氮化反应制备了CeN粉末。整个反应过程分为水相过程和热处理过程。水相过程主要是Ce^(3+)和CA的螯合和聚酯,形成稳定的Ce^(3+)-CA螯合物前驱体。通过水相过程实现Ce... 详细信息

以柠檬酸(CA)为螯合剂,即以有机物为碳源,通过溶胶-凝胶法辅助碳热还原氮化反应制备了CeN粉末。整个反应过程分为水相过程和热处理过程。水相过程主要是Ce^(3+)和CA的螯合和聚酯,形成稳定的Ce^(3+)-CA螯合物前驱体。通过水相过程实现Ce源与C源在分子水平上的均匀混合。热处理过程包括原位碳化和碳热还原氮化反应两部分。原位碳化过程形成的CeO_(2)/C粉末促进了CeO_(2)和C之间的紧密接触,从而减少Ce源和C源原子的扩散距离,以促进碳热还原氮化过程。

关键词：溶胶-凝胶原位碳化碳热还原氮化反应 CeN粉末

Administrative Methods on Finished Vehicles Featuring Import Parts——promulgation, operation and trade disputes caused

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中国汽车（英文版） 2008年第6期 9-10页

作者： JI Xuehong, CAI guoqin, liu Di

I Enactment of the administrative methods In the end of 2001, China became a member of the World Trade Organization and cancelled some limits on vehicle import. As tariffs of auto parts became lower than that of complete vehicles, partial joint ventures began assembling complete vehicles KD parts and a lot of KD parts came in then.

关键词： Administrative import promulgation administrative