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Journal of Remote Sensing 2021年第1期2021卷 289-301页

作者： guomei wei Zhongping Lee Xiuling Wu Xiaolong Yu Shaoling Shang Ricardo Letelier State Key Laboratory of Marine Environmental Science College of Ocean and Earth SciencesXiamen UniversityXiamen 361102China Research and Development Center for Ocean Observation Technologies Xiamen UniversityXiamen 361102China School for the Environment University of Massachusetts BostonBostonMA 02125USA College of Earth Ocean&Atmospheric SciencesOregon State UniversityCorvallisOR 97331USA

There has been a long history of interest on how(if)the absorption coefficient of“pure”fresh water(a_(fw)(λ))and“pure”seawater(a_(sw)(λ))changes with temperature(T),yet the impact of T reported in the literature differs significantly in the blue *** the previous studies based on laboratory measurements,we took an approach based on~18 years(2002–2020)of MODIS ocean color and temperature measurements in the oligotrophic oceans,along with field measured chlorophyll concentration and phytoplankton absorption coefficient,to examine the relationship between T and the total absorption coefficient(a(λ))at 412 and 443 *** found that the values of a(412)and a(443)in the summer are nearly flat(slightly decreasing)for the observed T range of~19–27℃.Since there are no detectable changes of chlorophyll during this period,the results suggest that T has a negligible impact on a_(sw)(412) and a_(sw)(443)in this T *** a complement,the impact of salinity on a_(fw)(λ) was also evaluated using three independent determinations of a_(sw)(λ) and a_(fw)(λ),where good agreements were found from these observations.

关键词： salinity ocean coefficient

基于固有光学特性的北部湾透明度遥感反演及其检验

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高技术通讯 2009年第9期19卷 977-982页

作者：魏国妹商少凌李忠平兰健厦门大学近海海洋环境科学国家重点实验室厦门361005 Northern Gulf Institute Mississippi State Universityms39529 中国海洋大学海洋系青岛266100

综合了固有光学量的反演和透明度与固有光学量的关系,构成了基于固有光学特性的透明度遥感算法。将此算法应用于北部湾的MODIS遥感数据,得到了该区域的透明度,并利用实测透明度对遥感反演结果进行了验证。通过对时间窗口为±48h,空间窗... 详细信息

综合了固有光学量的反演和透明度与固有光学量的关系,构成了基于固有光学特性的透明度遥感算法。将此算法应用于北部湾的MODIS遥感数据,得到了该区域的透明度,并利用实测透明度对遥感反演结果进行了验证。通过对时间窗口为±48h,空间窗口为1km的30组实测与遥感匹配数据的统计分析(实测透明度在1.8～26.0m范围),得到平均百分比误差(ε)为22%,对数均方根误差(1og_RMSE)为0.121。而基于叶绿素的经验算法反演的透明度,ε为42%,log_RMSE为0.185。上述结果说明,至少对于北部湾这类近岸水体,海色遥感结合基于固有光学量的算法,可以实现较高准确度的透明度遥感反演。

关键词：北部湾透明度半分析算法 MODIS

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国内食品菌落总数测定方法研究

福建分析测试 2008年第4期17卷 32-35页

作者：魏国美福建省中心检验所福建福州350002

主要叙述了有关食品中菌落测定的相关标准、方法、适用范围、区别以及其中存在的一些问题,提出了解决办法,从而为从事食品微生物检测提供一些参考。

关键词：食品菌落总数测定方法

Evolutionary game analysis of shared parking market diffusion under government management

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Transportation Safety and Environment 2024年第3期6卷 17-27页

作者： Qingqi wei guomei Xiao School of Economics and Management Chongqing Jiaotong UniversityChongqing 400074China

The imbalance between supply and demand in urban settings poses a significant barrier to the sustainable advancement of urban *** parking serves as a viable solution to mitigate these ***,for its sustained growth,a regulatory mechanism enforced by the government is *** promote shared parking market diffusion,we construct an evolutionary game model that incorporates the government,enterprises and parking *** explores stabilization strategies for these stakeholders and identifies multiple equilibrium states under different parameter *** results show that the rate and stability of these evolutionary strategies are constrained by the mutual benefits derived by the three ***,such stakeholders are reciprocally influenced by their willingness to engage in shared parking to varying *** subsidies serve as a determining factor for the strategic choices made by both enterprises and demanders,albeit at different evolutionary *** who have a higher value of time tend to park on-street,thereby influencing enterprise *** foster the long-term growth of the shared parking market,the government must enact appropriate subsidy policies,maintain consistent regulations and advocate for increased subsidies for parking demanders to reduce the effect of temporal heterogeneity on parking behavioural choices.

关键词： traffic management shared parking government management parking behaviour choice evolutionary game

胞内分枝杆菌临床分离株耐药谱及基因型特征研究

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中华结核和呼吸杂志 2018年第7期41卷 539-543页

作者：王淑琦姜广路魏国梅霍凤敏董玲玲赵立平黄海荣王桂荣首都医科大学附属北京胸科医院北京市结核病胸部肿瘤研究所国家结核病临床实验室北京市耐药结核病研究重点实验室 101149

目的分析我国胞内分枝杆菌临床分离株的耐药谱及基因型特征，为治疗胞内分枝杆菌感染性疾病提供科学依据。方法纳入2013—2015年北京胸科医院150株胞内分枝杆菌临床分离株，采用微孔板Alamar Blue法测定胞内分枝杆菌对15种药物的最低抑... 详细信息

目的分析我国胞内分枝杆菌临床分离株的耐药谱及基因型特征，为治疗胞内分枝杆菌感染性疾病提供科学依据。方法纳入2013—2015年北京胸科医院150株胞内分枝杆菌临床分离株，采用微孔板Alamar Blue法测定胞内分枝杆菌对15种药物的最低抑菌浓度（MIC），以确定其药物的敏感度；对16个可变数目串联重复序列（VNTR）位点进行PCR扩增和产物电泳分析，使用BioNumerics软件对菌株进行聚类分析，以确定其基因型特征。使用SPSS 19.0软件对结果进行统计分析，应用χ2检验分析不同组间耐药率的差别。结果药敏试验结果显示，克拉霉素（97.3%，146/150）、莫西沙星（94.0%，141/150）和阿米卡星（90.0%，135/150）对胞内分枝杆菌具有较好的抗菌活性；75.3%（113/150）、64.0%（96/150）、52.7%（79/150）和8.7%（13/150）的菌株对利福平、利奈唑胺、卷曲霉素和乙胺丁醇敏感；3种注射类抗结核药物的MIC50与MIC90值为：阿米卡星4和16 mg/L，链霉素4和16 mg/L，卷曲霉素8和16 mg/L；5种氟喹诺酮类药物的MIC50与MIC90值为：莫西沙星0.5和2 mg/L，环丙沙星1和8 mg/L，左氧氟沙星1和8 mg/L，安妥沙星2和16 mg/L，氧氟沙星2和16 mg/L。采用16位点VNTR方法对胞内分枝杆菌进行基因分型，150株胞内分枝杆菌共分为21个簇，121种基因型，总Hunter－Gaston指数为0.997。结论克拉霉素、莫西沙星和阿米卡星在体外对胞内分枝杆菌具有较好的抗菌活性；16位点VNTR方法对胞内分枝杆菌临床分离株的分辨率较高；胞内分枝杆菌的耐药谱与菌株是否成簇并无明显相关性。

关键词：胞内分枝杆菌最低抑菌浓度抗药性基因型

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分光光度计鉴别结核与非结核分枝杆菌的研究

继续医学教育 2019年第4期33卷 133-135页

作者：魏国梅王桂荣姜广路于霞陈素婷黄海荣天津市第二人民医院检验科天津300192 首都医科大学附属北京胸科医院/北京市结核病胸部肿瘤研究所国家结核病临床实验室/北京市耐药结核病研究重点实验室北京101149

目的建立并评估应用分光光度计测定对氨基苯甲酸(PABA的浓度,快速鉴别结核分枝杆菌(MTB)与非结核分枝杆菌(NTM)的方法。方法首先建立应用分光光度计测定PABA浓度的方法,然后以传统含对硝基苯甲酸(PNB)罗氏培养基鉴定结果为参照,应用32株... 详细信息

目的建立并评估应用分光光度计测定对氨基苯甲酸(PABA的浓度,快速鉴别结核分枝杆菌(MTB)与非结核分枝杆菌(NTM)的方法。方法首先建立应用分光光度计测定PABA浓度的方法,然后以传统含对硝基苯甲酸(PNB)罗氏培养基鉴定结果为参照,应用32株MTB临床分离株和33株NTM临床分离株评价该方法的准确性。结果以PABA浓度0μg/mL定为cut-off值,还原PNB产生PABA的浓度大于0μg/mL判定为NTM,小于0μg/mL判定为MTB,应用分光光度计测定PABA浓度法鉴定32株MTB临床分离株和33株NTM临床分离株的结果与传统鉴定法完全一致,其灵敏性和特异性均为100%。结论本研究建立了应用分光光度计测定PABA浓度快速鉴别MTB与NTM的方法,该方法简便、快速、廉价,并具有高度的特异性和敏感性,可作为鉴别MTB与NTM的有价值的检测手段。

关键词：结核分枝杆菌非结核分枝杆菌分光光度计对硝基苯甲酸对氨基苯甲酸

胞内分枝杆菌临床分离株耐药谱及基因型特征研究

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结核病与胸部肿瘤 2018年第4期 238-242页

目的分析我国胞内分枝杆菌临床分离株的耐药谱及基因型特征,为治疗胞内分枝杆菌感染性疾病提供科学依据。方法纳入2013-2015年北京胸科医院150株胞内分枝杆菌临床分离株,采用微孔板Alamar Blue法测定胞内分枝杆菌对15种药物的最低抑菌浓... 详细信息

目的分析我国胞内分枝杆菌临床分离株的耐药谱及基因型特征,为治疗胞内分枝杆菌感染性疾病提供科学依据。方法纳入2013-2015年北京胸科医院150株胞内分枝杆菌临床分离株,采用微孔板Alamar Blue法测定胞内分枝杆菌对15种药物的最低抑菌浓度(MIC),以确定其药物的敏感度;对16个可变数目串联重复序列(VNTR)位点进行PCR扩增和产物电泳分析,使用BioNumerics软件对菌株进行聚类分析,以确定其基因型特征。使用SPSS19.0软件对结果进行统计分析,应用Х^2检验分析不同组间耐药率的差别。结果药敏试验结果显示,克拉霉素(97.3%,146/150)、莫西沙星(94.0%,141/150)和阿米卡星(90.0%,135/150)对胞内分枝杆菌具有较好的抗菌活性;75.3%(113/150)、64.0%(96/150)、52.7%(79/150)和8.7%(13/150)的菌株对利福平、利奈哩胺、卷曲霉素和乙胺丁醇敏感;3种注射类抗结核药物的MIC50与MIC90值为:阿米卡星4和16mg/L,链霉素4和16mg/L,卷曲霉素8和16mg/L;5种氟座诺酮类药物的MIC50与MIC90值为:莫西沙星0.5和2mg/L,环丙沙星1和8mg/L,左氧氟沙星1和8mg/L,安妥沙星2和16mg/L,氧氟沙星2和16mg/L。采用16位点VNTR方法对胞内分枝杆菌进行基因分型,150株胞内分枝杆菌共分为21个簇,121种基因型,总Hunter-Gaston指数为0.997。结论克拉霉素、莫西沙星和阿米卡星在体外对胞内分枝杆菌具有较好的抗菌活性;16位点VNTR方法对胞内分枝杆菌临床分离株的分辨率较高;胞内分枝杆菌的耐药谱与菌株是否成簇并无明显相关性。

关键词：胞内分枝杆菌最低抑菌浓度抗药性基因型

Generation of coexisting high-energy pulses in a mode-locked all-fiber laser with a nonlinear multimodal interference technique

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Photonics Research 2019年第2期7卷 187-192页

作者： GUANGwei CHEN WENLEI LI guomei WANG WENFU ZHANG CHAO ZENG wei ZHAO State Key Laboratory of Transient Optics and Photonics Xi’an Institute of Optics and Precision MechanicsChinese Academy of Sciences University of Chinese Academy of Sciences (UCAS)

We demonstrate a passively mode-locked all-fiber laser incorporating a piece of graded-index multimode fiber as a mode-locking modulator based on a nonlinear multimodal interference technique, which generates two types of coexisting high-energy ultrashort pulses [i.e., the conventional soliton(CS) and the stretched pulse(SP)]. The CS with pulse energy as high as 0.38 n J is obtained at the pump level of 130 mW. When the pump increases to175 mW, the high-energy SP occurs at a suitable nonlinear phase bias and its pulse energy can reach 4 n J at a 610 mW pump. The pulse durations of the generated CS and SP are 2.3 ps and 387 fs, respectively. The theory of nonlinear fiber optics, single-shot spectral measurement by the dispersive Fourier-transform technique, and simulation methods based on the Ginzburg–Landau equation are provided to characterize the laser physics and reveal the underlying principles of the generated CS and SP. A rogue wave, observed between the CS and SP regions, mirrors the laser physics behind the dynamics of generating a high-energy SP from a CS. The proposed all-fiber laser is versatile, cost-effective and easy to integrate, which provides a promising solution for high-energy pulse generation.

关键词： Generation high-energy pulses

Single-cell immune landscape of measurable residual disease in acute myeloid leukemia

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Science China(Life Sciences) 2024年第11期67卷 2309-2322页

作者： Xiaodong Mo weilong Zhang guomei Fu Yingjun Chang Xiaohui Zhang Lanping Xu Yu Wang Chenhua Yan Mengzhu Shen Qiuxia wei Changjian Yan Xiaojun Huang Peking University People’s Hospital Peking University Institute of HematologyNational Clinical Research Center for Hematologic DiseaseBeijing Key Laboratory of Hematopoietic Stem Cell TransplantationBeijing 100044China Department of Hematology Lymphoma Research CenterPeking University Third HospitalBeijing100191China Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijing 100044China Research Unit of Key Technique for Diagnosis and Treatments of Hematologic Malignancies Chinese Academy of Medical SciencesBeijing 100044China

Measurable residual disease(MRD)is a powerful prognostic factor of relapse in acute myeloid leukemia(AML).We applied the single-cell RNA sequencing to bone marrow(BM)samples from patients with(n=20)and without(n=12)MRD after allogeneic hematopoietic stem cell transplantation.A comprehensive immune landscape with 184,231 cells was *** with CD8+T cells enriched in the MRDnegative group(MRD‒_CD8),those enriched in the MRD-positive group(MRD+_CD8)showed lower expression levels of cytotoxicity-related *** monocyte clusters(i.e.,MRD+_M)and three B-cell clusters(i.e.,MRD+_B)were enriched in the MRD-positive *** from an MRD-positive state to an MRD-negative state was accompanied by an increase in MRD‒_CD8 clusters and vice *** cell clusters employed the macrophage migration inhibitory factor pathway to regulate MRD‒_CD8 *** findings revealed the characteristics of the immune cell landscape in MRD positivity,which will allow for a better understanding of the immune mechanisms for MRD conversion.

关键词： acute myeloid leukemia measurable residual disease immune landscape single-cell RNA sequencing allogeneic hematopoietic stem cell transplantation

LSTM Network based Spoofing Detection and Recognition in a GNSS Receiver

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LSTM Network based Spoofing Detection and Recognition in a G...