检索结果-南通市图书馆

Association of growth rate with hormone levels and myogenic gene expression profile in broilers

在线全文

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2017年第3期8卷 561-567页

作者： Yingping xiao Choufei Wu Kaifeng Li guohong Gui Guolong Zhang Hua Yang Institute of Quality and Standards for Agro-products Zhejiang Academy of Agricultural Sciences College of Life Sciences Huzhou University Department of Animal Science Oklahoma State University

Background： The growth rate often varies among individual broilers of the same breed under a common management condition. To investigate whether a variation in the growth rate is associated with a difference in hormone levels and myogenic gene expression profile in broilers, a feeding trial was conducted with 10,000 newly hatched Ross 308 chicks in a commercial production facility under standard management. At 38 d of age,30 fast-, 30 medium-, and 30 slow-growing broilers were selected among 600 healthy male individuals. The levels of insulin-like growth factor-1（IGF-1）, triiodothyronine（T3）, thyroxine（T4）, and growth hormone in the serum or breast muscle were assayed by ELISA or RIA kits, and the expression levels of several representative pro-and anti-myogenic genes in the breast muscle were also measured by real-time ***： Results showed that both absolute and relative weights of the breast muscle were in linear positive correlations with the body weight of broilers（P 〈 0.001）. Fast-growing broilers had higher concentrations of IGF-1 than slow-growing broilers（P 〈 0.05） in both the serum and breast muscle. The serum concentration of T3 was significantly higher in fast-growing birds than in slow-growing birds（P 〈 0.05）. However, no difference was observed in growth hormone or T4 concentration among three groups of birds. Additionally, a decreased expression of an anti-myogenic gene（myostatin） and increased expressions of pro-myogenic genes such as myogenic differentiation factor 1, myogenin, muscle regulatory factor 4, myogenic factor 5, IGF-1, and myocyte enhancer factor 2B, C, and D were observed in fast-growing broilers（P 〈 0.05）, relative to slow-growing ***： Collectively, these findings suggested that the growth rate is linked to the hormone and myogenic gene expression levels in broiler chickens. Some of these parameters such as serum concentrations of IGF-1 and T3 could be employed to breed for enhanced growth.

关键词： Breast muscle Broiler Growth performance Hormone Myogenic gene expression

改造Cry1Ac蛋白C端对转基因水稻抗二化螟虫的影响

在线全文

学校读者我要写书评

暂无评论

分子植物育种 2017年第4期15卷 1312-1319页

作者：刘洋刘相国闫玮玉金永梅陈子奇贾伟肖国红张艳林秀峰尹悦佳吉林省农业科学院农业生物技术研究所吉林省农业生物技术重点实验室长春130033 东北师范大学长春130024 哈尔滨师范大学生命科学与技术学院哈尔滨150025 吉林农业大学生命科学学院长春130118

二化螟虫的泛滥严重影响水稻的产量,cry1Ac基因是目前世界上应用最广泛和最高效的抗虫基因之一,但二化螟虫通过进化会逐渐对其产生抗性。通过改变蛋白结构来构建新的Cry蛋白是解决这一问题的有效途径之一。为分析改造Cry1Ac蛋白C端能否... 详细信息

二化螟虫的泛滥严重影响水稻的产量,cry1Ac基因是目前世界上应用最广泛和最高效的抗虫基因之一,但二化螟虫通过进化会逐渐对其产生抗性。通过改变蛋白结构来构建新的Cry蛋白是解决这一问题的有效途径之一。为分析改造Cry1Ac蛋白C端能否对转基因作物抗虫性产生影响,本研究采用Cry1Ja蛋白的C端替换Cry1Ac蛋白的C端,重组获得CryFLAc蛋白。将cry1Ac基因和编码CryFLAc蛋白的cryFLAc基因分别构建到pTF101.1-ubi植物表达载体上,利用农杆菌介导方法转化到吉林省水稻品种吉粳88中;采用PCR、RT-PCR、免疫检测试纸条检测的方法确定cry1Ac、cryFLAc基因整合和表达情况;通过室内和田间抗虫性测试评价CryFLAc和Cry1Ac蛋白对T1代转基因水稻抗虫性的影响。研究发现:cry1Ac、cryFLAc基因均成功整合到水稻基因组中,并稳定表达;转cryFLAc基因水稻抗二化螟水平达到抗性级别,转cry1Ac基因水稻达到高抗级别;转cry1Ac基因水稻二化螟抗性高于转cryFLAc基因水稻。结果表明:Cry1Ja蛋白的C端替换Cry1Ac蛋白的C端不会提高转基因水稻的抗虫性。上述研究为人工设计合理化改造Cry蛋白提供了新的理论依据。

关键词： Cry1Ac Cry1Ja C端转基因水稻抗虫性

在线全文

学校读者我要写书评

暂无评论

高等医学教育的临床实践教学改革探讨

继续医学教育 2016年第8期30卷 17-19页

作者：肖国宏阮静贤宋霁金晶晶白诗雨夏承来杨洁广州医科大学第三临床学院妇产科广东广州510150 广州医科大学附属第三医院生殖医学中心广东广州510150

高等医学教育是培养医学人才的必由之路,临床实践教学是培养合格的临床医学人才至关重要的培养过程。通过临床医学本科毕业生实习情况调查,为探讨临床实践教学方法改革提供思路。

关键词：医学教育临床实践教学教学改革

在线全文

学校读者我要写书评

暂无评论

注水管线酸洗除垢工艺

清洗世界 2004年第6期20卷 1-3页

作者：杨建华肖国洪王怀玲李伟滨央乃昌中原油田分公司采油二厂河南范县457532

油田注水管线结垢,会降低管线的流量。针对濮城油田注水井回注污水的特点,提出了一种新的注水管线酸洗除垢工艺。通过对管垢样的分析和药剂配方的室内研究及现场试验表明该工艺简单、施工方便、费用低,取得了良好的增注效果。

关键词：濮城油田地面管线除垢工艺药剂配方回注污水酸洗过程物化分析

A Climatic Sequence Stratigraphic Model in the Terrestrial Lacustrine Basin:A Case Study of Green River Formation,Uinta Basin,USA

在线全文

学校读者我要写书评

暂无评论

Acta Geologica Sinica(English Edition) 2009年第1期83卷 130-135页

作者： WANG Junling ZHENG Herong xiao Huanqin ZHONG guohong Ronald STEEL YIN Peigui Petroleum Exploration and Production Institute SINOPEC Beijing 10083 China Institute ofGeoscience Shengli Oilfield Company Limited SINOPEC Beijing 10083 China Geology Department University of Texas Austin Texas USA Energy Research Institute University of Wyoming Laramie WY82071 USA

In recent years, with the development of terrestrial sequence stratigraphy, more attention has been focused on the study of the terrestrial lacustrine sequence stratigraphic model globally. Different viewpoints are preferred by researchers. Under the guidance of the theory of sequence stratigraphy, the findings of this paper indicate that climate is a major factor controlling the formation of the fourth-order sequence, based upon the study of the sequence stratigraphy in the Green River Formation of the Uinta basin in the USA. It also divides the fourth-order sequence in the terrestrial lacustrine basin into two system tracts： the wet （rising） half-cycle and the dry （falling） half- cycle, establishing a new-style fourth-order sequence stratigraphic model for the terrestrial lacustrine basin, that is, the climate-genetic sequence stratigraphic model. As a result, the theory of sequence stratigraphy is greatly enriched.

关键词： climate fourth-order sequence sequence stratigraphic model terrestrial lacustrine basin,Green River Formation Uinta basin

Atomic-level insight of sulfidation-engineered Aurivillius-related Bi_(2)O_(2)SiO_(3) nanosheets enabling visible light low-concentration CO_(2) conversion

在线全文

学校读者我要写书评

暂无评论

Carbon Energy 2023年第2期5卷 1-11页

作者： Kai Wang Yue Du Yuan Li xiaoyong Wu Haiyan Hu guohong Wang Yao xiao Shulei Chou Gaoke Zhang College of Urban and Environmental Sciences Hubei Key Laboratory of Pollutant Analysis and Reuse TechnologyInstitute for Advanced MaterialsHubei Normal UniversityHuangshiHubeiChina School of Resources and Environmental Engineering Wuhan University of TechnologyWuhanHubeiChina Institute for Carbon Neutralization College of Chemistry and Materials EngineeringWenzhou UniversityWenzhouZhejiangChina

Unraveling atomic-level active sites of layered photocatalyst towards lowconcentration CO_(2) conversion is still ***,the yield and selectivity of photocatalytic CO_(2) reduction of the Aurivillius-related oxide semiconductor Bi_(2)O_(2)SiO_(3) nanosheet(BOSO)were largely improved using a surface sulfidation *** experiment and theoretical calculation confirmed that surface sulfidation of the Bi_(2)O_(2)SiO_(3) nanosheet(S-BOSO,6.28 nm)redistributed the charge-enriched Bi sites,extended the solar spectrum absorption to the whole visible range,and considerably enhanced the charge separation,in addition to creating new reaction active sites,as compared to pristine ***,surface sulfidation played a switchable role,wherein S-BOSO showed a very high CH_(3)OH generation rate(12.78μmol g^(-1) for 4 h,78.6%selectivity)from low-concentration CO_(2)(1000 ppm)under visible light irradiation,which outperforms most of the state-of-the-art photocatalysts under similar *** study presents an atomic-level modification protocol for engineering reactive sites and charge behaviors to promote solar-to-energy conversion.

关键词： [Bi_(2)O_(2)]^(2)+layer Bi_(2)O_(2)SiO_(3) low-concentration CO_(2)reduction photocatalysis sulfidation

Weld Geometry Monitoring for Metal Inert Gas Welding Process with Galvanized Steel Plates Using Bayesian Network

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Shanghai Jiaotong university(Science) 2021年第2期26卷 239-244页

作者： MA guohong LI Jian HE Yinshui xiao Wenbo Key Laboratory of Lightweight and High Strength Structural Materials of Jiangxi Province School of Mechanical and Electrical EngineeringNanchang UniversityNanchang 330031China School of Environment and Chemical Engineering Nanchang UniversityNanchang 330031China Key Laboratory of Nondestructive Testing Ministry of Education Nanchang Hangkong UniversityNanchang 330063China

We present a novel method to monitor the weld geometry for metal inert gas(MIG)welding process with galvanized steel plates using Bayesian network(BN),and propose an effective method of extracting the weld reinforcement and width *** laser vision sensor is mounted after the welding torch and used to profile the *** the extracted weld geometry and the adopted process parameters,a back propagation neural network(BPNN)is constructed offline and used to predict the weld reinforcement and width corresponding to the current parameter settings.A BN from welding experience and tests is presented to implement the decision making of welding current/voltage when the error between the predictive geometry and the actual one *** study can deal with the negative welding tendency to adapt to welding randomness and indicates a valuable application prospect in the welding field.

关键词： galvanized steel plate weld geometry laser vision sensor Bayesian network(BN) back propagation neural network(BPNN)

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

广西高龙穹窿构造蚀变体特征与找矿潜力

黄金 2023年第11期44卷 83-88页

作者：龙成雄刘建中杨成富汪小勇肖国洪胡新来秦琴贵州省地质矿产勘查开发局一0五地质大队自然资源部基岩区矿产资源勘查工程技术创新中心贵州省地质矿产勘查开发局黔西南金龙黄金矿业有限责任公司中国黄金集团广西有限公司贵州煤矿地质工程咨询与地质环境监测中心

高龙穹窿分布多个矿体,其周边的环状断裂形成矿体。随着多层次构造蚀变体的研究理论在黔西南地区金找矿中取得重大成果,认为热液在背斜(穹窿)核部附近的不整合界面或者能干性差异较大的岩层面易形成多层次构造蚀变体。通过在高龙穹窿首... 详细信息

高龙穹窿分布多个矿体,其周边的环状断裂形成矿体。随着多层次构造蚀变体的研究理论在黔西南地区金找矿中取得重大成果,认为热液在背斜(穹窿)核部附近的不整合界面或者能干性差异较大的岩层面易形成多层次构造蚀变体。通过在高龙穹窿首次进行构造蚀变体特征研究,对矿体特征、分布状况、环状断裂进行了重新认识,最终对找矿潜力进行了分析,该成果可为高龙穹窿进一步找矿提供依据。

关键词：高龙穹窿构造蚀变体卡林型金矿床找矿潜力矿体特征环状断裂

Expansion and adaptive evolution of the WRKY transcription factor family in Avicennia mangrove trees

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Marine Life Science & Technology 2023年第2期5卷 155-168页

作者： xiao Feng guohong Li Weihong Wu Haomin Lyu Jiexin Wang Cong Liu Cairong Zhong Suhua Shi Ziwen He State Key Laboratory of Biocontrol Guangdong Key Laboratory of Plant ResourcesSchool of Life SciencesSouthern Marine Science and Engineering Guangdong Laboratory(Zhuhai)Sun Yat-sen UniversityGuangzhou 510275China Greater Bay Area Institute of Precision Medicine(Guangzhou) Fudan UniversityGuangzhou 511458China Hainan Academy of Forestry(Hainan Academy of Mangrove) Haikou 571100China

Mangroves are adapted to intertidal zones,which present extreme environmental *** are among the most prominent transcription factors(TFs)in higher plants and act through various interconnected networks to regulate responses to multiple abiotic ***,based on omic data,we investigated the landscape and evolutionary patterns of WRKYs in the main mangrove genus *** found that both the number and the proportion of TFs and WRKYs in Avicennia species exceeded their inland relatives,indicating a signifcant expansion of WRKYs in *** identifed 109 WRKY genes in the representative species Avicennia *** genomic analysis showed that two recent wholegenome duplication(WGD)events played a critical role in the expansion of WRKYs,and 88%of Avicennia marina WRKYs(AmWRKYs)have been retained following these *** comparative transcriptomics on roots under experimental salt gradients,we inferred that there is high divergence in the expression of WGD-retained ***,we found that the expression of 16 AmWRKYs was stable between freshwater and moderately saline water but increased when the trees were exposed to high *** particular,14 duplicates were retained following the two recent WGD events,indicating potential neo-and *** also found that WRKYs could interact with other upregulated genes involved in signalling pathways and natural antioxidant biosynthesis to enhance salt tolerance,contributing to the adaptation to intertidal *** omic data of the WRKY family in *** broadens the understanding of how a TF family relates to the adaptive evolution of mangroves.

关键词： Adaptation Avicennia Mangrove Transcription factor Whole-genome duplication WRKY