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基于游离小孢子培养的菜心DH系评价

长江蔬菜 2024年第20期 48-52页

作者：庞强强孙晓东周曼蔡兴来王亚强施国宾海南省农业科学院蔬菜研究所/海南省蔬菜生物学重点实验室/海南省院士团队创新中心/海南省瓜菜育种工程技术研究中心海南海口571100

为筛选优良菜心DH系,采用田间试验方法,对游离小孢子培养获得的再生植株(DH系)的园艺学性状进行观察和鉴定。结果表明,获得了41份菜心DH系,经综合评价筛选出12个综合性状表现较好的DH系。这些材料的获得有助于丰富菜心种质资源,可为杂... 详细信息

为筛选优良菜心DH系,采用田间试验方法,对游离小孢子培养获得的再生植株(DH系)的园艺学性状进行观察和鉴定。结果表明,获得了41份菜心DH系,经综合评价筛选出12个综合性状表现较好的DH系。这些材料的获得有助于丰富菜心种质资源,可为杂交亲本的选择和配组提供参考。

关键词：菜心 DH系园艺性状综合评价

Inactivated H9N2 vaccines developed with early strains do not protect against recent H9N2 viruses:Call for a change in H9N2 control policy

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Journal of Integrative Agriculture 2024年第6期23卷 2144-2148页

作者： Yanjing Liu Qingqing Yu Xiangyu Zhou Wenxin Li Xinwen He Yan Wang Guohua Deng Jianzhong shi guobin Tian Xianying Zeng Hualan Chen College of Veterinary Medicine Gansu Agricultural UniversityLanzhou 730070China State Key Laboratory for Animal Disease Control and Prevention Key Laboratory for Prevention and Control of Avian Influenza and Other Major Poultry DiseasesMinistry of Agriculture and Rural Affairs/Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150009China National Poultry Laboratory Animal Resource Center Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150009China Harbin Weike Biotechnology Co. Ltd.Harbin 150009China

H9N2 virus has been widely distributed in wild birds and poultry around the world since its first emergence in the United States of America in 1966(Gu et al.2017;Carnaccini and Perez 2020).The virus appeared in chickens in China in the early 1990s,and over the last two decades has gradually become the dominant epidemic subtype(Sun and Liu 2015;Bi et al.2020).Although H9N2 virus infection alone cannot cause severe disease or death in poultry,H9N2 virus-infected birds experience a degree of egg production drop and can be easily infected by other pathogens,thus causing economic losses for poultry industry.

关键词： H9N2 poultry policy

M6PR interacts with the HA2 subunit of influenza A virus to facilitate the fusion of viral and endosomal membranes

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Science China(Life Sciences) 2024年第3期67卷 579-595页

作者： Yuzhen Hu Li Jiang Guangwen Wang Yangming Song Zhibo Shan Xuyuan Wang Guohua Deng Jianzhong shi guobin Tian Xianying Zeng Liling Liu Hualan Chen Chengjun Li State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China

Influenza A virus(IAV) commandeers numerous host cellular factors for successful replication. However, very few host factors have been revealed to be involved in the fusion of viral envelope and late endosomal membranes. In this study, we identified cation-dependent mannose-6-phosphate receptor(M6PR) as a crucial host factor for the replication of IAV. We found that siRNA knockdown of M6PR expression significantly reduced the growth titers of different subtypes of IAV, and that the inhibitory effect of M6PR siRNA treatment on IAV growth was overcome by the complement of exogenously expressed M6PR. When A549 cells were treated with siRNA targeting M6PR,the nuclear accumulation of viral nucleoprotein(NP) was dramatically inhibited at early timepoints post-infection, indicating that M6PR engages in the early stage of the IAV replication cycle. By investigating the role of M6PR in the individual entry and post-entry steps of IAV replication, we found that the downregulation of M6PR expression had no effect on attachment, internalization, early endosome trafficking,or late endosome acidification. However, we found that M6PR expression was critical for the fusion of viral envelope and late endosomal membranes. Of note, M6PR interacted with the hemagglutinin(HA) protein of IAV, and further studies showed that the lumenal domain of M6PR and the ectodomain of HA2 mediated the interaction and directly promoted the fusion of the viral and late endosomal membranes,thereby facilitating IAV replication. Together, our findings highlight the importance of the M6PR–HA interaction in the fusion of viral and late endosomal membranes during IAV replication.

关键词： influenza A virus M6PR HA membrane fusion late endosome

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农作物图像及温湿度无线远程监测系统设计

农机化研究 2011年第4期33卷 167-170页

作者：史国滨王熙黑龙江八一农垦大学黑龙江大庆163319

利用嵌入式系统进行数据采集处理,结合GPRS网络进行数据传输的技术日益成为现今数据采集的趋势。为此,对远程数据采集和无线网络传输在农作物图像及温湿度监测的系统进行了研究,实现了此项应用需求。硬件采用C8051F单片机,将远程农田作... 详细信息

利用嵌入式系统进行数据采集处理,结合GPRS网络进行数据传输的技术日益成为现今数据采集的趋势。为此,对远程数据采集和无线网络传输在农作物图像及温湿度监测的系统进行了研究,实现了此项应用需求。硬件采用C8051F单片机,将远程农田作物的图像数据和温湿度监测数据进行格式化封装,通过GPRS网络以SOCKET方式进行网络通信实现数据远程无线传输。该系统在现代农业远程监控领域具有广泛的应用前景。

关键词：无线监测数据封装图像传输

Real-world effectiveness of early insulin therapy on the incidence of cardiovascular events in newly diagnosed type 2 diabetes

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Signal Transduction and Targeted Therapy 2024年第7期9卷 2953-2959页

作者： Sihui Luo Xueying Zheng Wei Bao Sheng Nie Yu Ding Tong Yue Yilun Zhou Ying Hu Hua Li Qiongqiong Yang Qijun Wan Bicheng Liu Hong Xu Guisen Li Gang Xu Chunbo Chen Huafeng Liu Yongjun shi Yan Zha Yaozhong Kong guobin Su Ying Tang Mengchun Gong Linong Ji Fan Fan Hou Jianping Weng Department of Endocrinology First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHeifeiChina Institute of Public Health Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiChina Division of Nephrology National Clinical Research Center for Kidney DiseaseState Key Laboratory of Organ Failure ResearchNanfang HospitalSouthern Medical UniversityGuangzhouChina Department of Nephrology Beijing Tiantan HospitalCapital Medical UniversityBeijingChina The Second Affiliated Hospital of Zhejiang University School of Medicine HangzhouChina Sir Run Run Shaw Hospital Zhejiang University School of MedicineHangzhouChina Department of Nephrology Sun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhouChina The Second People’s Hospital of Shenzhen Shenzhen UniversityShenzhenChina Institute of Nephrology Zhongda HospitalSoutheast University School of MedicineNanjingChina Children’s Hospital of Fudan University ShanghaiChina Renal Department and Institute of Nephrology Sichuan Provincial People’s HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaSichuan Clinical Research Center for Kidney DiseasesChengduChina Division of Nephrology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Department of Critical Care Medicine Maoming People’s HospitalMaomingChina Key Laboratory of Prevention and Management of Chronic Kidney Disease of Zhanjiang City Institute of NephrologyAffiliated Hospital of Guangdong Medical UniversityZhanjiangChina Huizhou Central People’s Hospital Sun Yat-Sen UniversityHuizhouChina Guizhou Provincial People’s Hospital Guizhou UniversityGuiyangChina Department of Nephrology the First People’s Hospital of FoshanFoshanChina Department of Nephrology Guangdong Provincial Hospital of Chinese Medicinethe Second Affiliated Hospitalthe Second Clinical CollegeGuangzhou University of Chinese Medicin

Early insulin therapy is capable to achieve glycemic control and restoreβ-cell function in newly diagnosed type 2 diabetes(T2D),but its effect on cardiovascular outcomes in these patients remains *** this nationwide real-world study,we analyzed electronic health record data from 19 medical centers across China between 1 January 2000,and 26 May *** included 5424 eligible patients(mean age 56 years,2176 women/3248 men)who were diagnosed T2D within six months and did not have prior cardiovascular *** Cox regression models were used to estimate the associations of early insulin therapy(defined as the first-line therapy for at least two weeks in newly diagnosed T2D patients)with the incidence of major cardiovascular events including coronary heart disease(CHD),stroke,and hospitalization for heart failure(HF).During 17,158 persons years of observation,we documented 834 incident CHD cases,719 stroke cases,and 230 hospitalized cases for *** diagnosed T2D patients who received early insulin therapy,compared with those who did not receive such treatment,had 31%lower risk of incident stroke,and 28%lower risk of hospitalization for *** significant difference in the risk of CHD was *** found similar results when repeating the aforesaid analysis in a propensity-score matched population of 4578 patients and with inverse probability of treatment weighting *** findings suggest that early insulin therapy in newly diagnosed T2D may have cardiovascular benefits by reducing the risk of incident stroke and hospitalization for HF.

关键词： cardiovascular treatment nationwide

基于GPRS的温室图像与温湿度监测系统的实现

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黑龙江八一农垦大学学报 2011年第2期23卷 63-65,81页

作者：史国滨王熙黑龙江八一农垦大学现代教育技术中心大庆163319 黑龙江八一农垦大学工程学院

针对温室环境监测自动化这一发展趋势,提出了一种基于GPRS和WEB技术的远程监测系统。首先,通过单片机系统连接温湿度传感器、摄像头,并通过GPRS网络无线传输数据到监测中心,在数据库中存储并发布于WEB服务器上。系统软件实现了远程的通... 详细信息

针对温室环境监测自动化这一发展趋势,提出了一种基于GPRS和WEB技术的远程监测系统。首先,通过单片机系统连接温湿度传感器、摄像头,并通过GPRS网络无线传输数据到监测中心,在数据库中存储并发布于WEB服务器上。系统软件实现了远程的通信控制,并构建了基于***的B/S的"瘦客户"模式,通过浏览器实时浏览监测数据,并能进行历史数据查询。该系统的实现构建了完整的监测系统,为农业数据实时传输、监测提供了一种便捷的解决方案。

关键词：无线监测图像传输 WEB应用

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钢管拱桥钢筋混凝土拱肋施工

安徽建筑 2006年第3期13卷 96-98页

作者：陶红光史国斌安徽路达公路工程有限责任公司安徽安庆246003

介绍京杭运河大桥边跨拱肋支架的设计与施工,对上承式中小型中跨度拱桥施工方案的设计、不规则大型混凝土构件现浇支架的设计、现浇支架基础的处理、混凝土结构物三维线形的施工控制等方面进行探讨。

关键词：大桥边跨上承式拱桥拱肋拱肋支架设计与施工

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桥梁预应力砼管桩基础应用及研究

广东公路交通 2013年第1期39卷 23-26页

作者：孙向东黄琛石国彬广东省公路勘察规划设计院股份有限公司广州510507

介绍了预应力砼管桩的发展及应用,探讨制约桥梁管桩基础推广的主要因素,对规范体系、单桩竖向承载力、耐久性和抗震等问题进行探讨,并提出相关的技术措施。

关键词：预应力砼管桩竖向承载力耐久性抗震

提高储能VSG并网有功响应性能的暂态阻尼策略

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电力自动化设备 2024年第1期44卷 134-140页

作者：石荣亮张群英王国斌兰才华黄冀王斌桂林理工大学机械与控制工程学院广西桂林541004 广西壮族自治区特种设备检验研究院广西南宁530200 武汉科技大学信息科学与工程学院湖北武汉430081

储能虚拟同步发电机(VSG)的并网有功在有功指令与电网频率2种扰动下存在动态超调与稳态偏差。提出有功微分反馈补偿(ADFBC)和有功微分前馈补偿(ADFFC)2种暂态阻尼策略,并在分别建立典型VSG、ADFBC和ADFFC 3种策略并网有功闭环小信号模... 详细信息

储能虚拟同步发电机(VSG)的并网有功在有功指令与电网频率2种扰动下存在动态超调与稳态偏差。提出有功微分反馈补偿(ADFBC)和有功微分前馈补偿(ADFFC)2种暂态阻尼策略,并在分别建立典型VSG、ADFBC和ADFFC 3种策略并网有功闭环小信号模型的基础上,给出了相应的参数设计方法。仿真和实验对比结果均验证了所提ADFBC和ADFFC暂态阻尼策略可有效提高储能VSG并网有功响应,在2种扰动下其并网有功既无动态超调又无稳态偏差。

关键词：虚拟同步发电机动态超调稳态偏差有功微分反馈有功微分前馈小信号模型