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Nanomaterials for environmental burden reduction,waste treatment,and nonpoint source pollution control:a review

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Frontiers of Environmental Science & Engineering 2009年第3期3卷 249-264页

作者： guobin shan Rao Y.SURAMPALLI Rajeshwar D.TYAGI Tian C.ZHANG INRS-ETE University of QuebecQuebecG1K 9A9Canada U.S.Environmental Protection Agency Kansas CityKS 66117USA Civil Engineering University of Nebraska-LincolnOmahaNB 68182USA

Nanomaterials are applicable in the areas of reduction of environmental burden,reduction/treatment of industrial and agricultural wastes,and nonpoint source(NPS)pollution ***,environmental burden reduction involves green process and engineering,emissions control,desulfurization/denitrification of nonrenewable energy sources,and improvement of agriculture and food ***,reduction/treatment of industrial and agricultural wastes involves converting wastes into products,groundwater remediation,adsorption,delaying photocatalysis,and ***,NPS pollution control involves controlling water *** alter physical properties on a nanoscale due to their high specific surface area to volume *** are used as catalysts,adsorbents,membranes,and additives to increase activity and capability due to their high specific surface areas and nano-sized ***,nanomaterials are more effective at treating environmental wastes because they reduce the amount of material needed.

关键词： nanomaterials industrial agricultural nonpoint source pollution environmental burden reduction

M6PR interacts with the HA2 subunit of influenza A virus to facilitate the fusion of viral and endosomal membranes

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Science China(Life Sciences) 2024年第3期67卷 579-595页

作者： Yuzhen Hu Li Jiang Guangwen Wang Yangming Song Zhibo shan Xuyuan Wang Guohua Deng Jianzhong Shi guobin Tian Xianying Zeng Liling Liu Hualan Chen Chengjun Li State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China

Influenza A virus(IAV) commandeers numerous host cellular factors for successful replication. However, very few host factors have been revealed to be involved in the fusion of viral envelope and late endosomal membranes. In this study, we identified cation-dependent mannose-6-phosphate receptor(M6PR) as a crucial host factor for the replication of IAV. We found that siRNA knockdown of M6PR expression significantly reduced the growth titers of different subtypes of IAV, and that the inhibitory effect of M6PR siRNA treatment on IAV growth was overcome by the complement of exogenously expressed M6PR. When A549 cells were treated with siRNA targeting M6PR,the nuclear accumulation of viral nucleoprotein(NP) was dramatically inhibited at early timepoints post-infection, indicating that M6PR engages in the early stage of the IAV replication cycle. By investigating the role of M6PR in the individual entry and post-entry steps of IAV replication, we found that the downregulation of M6PR expression had no effect on attachment, internalization, early endosome trafficking,or late endosome acidification. However, we found that M6PR expression was critical for the fusion of viral envelope and late endosomal membranes. Of note, M6PR interacted with the hemagglutinin(HA) protein of IAV, and further studies showed that the lumenal domain of M6PR and the ectodomain of HA2 mediated the interaction and directly promoted the fusion of the viral and late endosomal membranes,thereby facilitating IAV replication. Together, our findings highlight the importance of the M6PR–HA interaction in the fusion of viral and late endosomal membranes during IAV replication.

关键词： influenza A virus M6PR HA membrane fusion late endosome

变性梯度凝胶电泳在环境微生物生态学中的应用

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生态学杂志 2006年第10期25卷 1257-1264页

作者：单国彬金文标林佶侃邢新会东莞宝丽美化工有限公司清华大学化学工程系生物化工研究所北京100084 哈尔滨工业大学深圳研究生院城市与土木工程学院金迪生物科技集团东莞523581 清华大学化学工程系生物化工研究所

PCR-变性梯度凝胶电泳(PCR-DGGE)具有可靠性强、重复性好、方便快捷等优点,已被广泛应用于环境生态学中微生物群落多样性、动态性分析和功能细菌的跟踪。本文综述了PCR-DGGE技术的基本原理,不同DNA提取方法的比较,不同PCR方式的比较及... 详细信息

PCR-变性梯度凝胶电泳(PCR-DGGE)具有可靠性强、重复性好、方便快捷等优点,已被广泛应用于环境生态学中微生物群落多样性、动态性分析和功能细菌的跟踪。本文综述了PCR-DGGE技术的基本原理,不同DNA提取方法的比较,不同PCR方式的比较及其在环境生态学中研究微生物群落多样性、环境中微生物群落变化的动态监测、硝化菌-反硝化菌和硫酸还原菌(SRB)的动态分析和监测等领域中的应用,并对该技术自身存在的局限性和应用前景进行了评价。

关键词： PCR-变性梯度凝胶电泳(PCR-DGGE) 废水生物处理微生物群落活性污泥生物膜硝化菌-反硝化菌硫酸还原菌

传统村落生态环境评价与保护发展研究——以广西钦州市大芦村为例

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环境与可持续发展 2015年第6期40卷 61-64页

作者：曾丽群单国彬朱鹏飞北京航空航天大学北海学院广西北海536000 广西科技大学鹿山学院广西柳州545000 广西城市设计有限公司广西北海536000

以中国传统村落和历史文化名村:广西钦州市大芦村为研究对象,结合土地利用调查GIS数据和相关的统计年鉴,采用《生态环境状况评价技术规范(HJ/T192-2006)》进行生态环境状况评价,计算出EI为84.90,生态环境状况为优。为了更加有效与系统... 详细信息

以中国传统村落和历史文化名村:广西钦州市大芦村为研究对象,结合土地利用调查GIS数据和相关的统计年鉴,采用《生态环境状况评价技术规范(HJ/T192-2006)》进行生态环境状况评价,计算出EI为84.90,生态环境状况为优。为了更加有效与系统的保护这一拥有丰富特色资源和优质生态环境的传统村落,需划定核心资源保护红线和村落生态环境保护红线,优化产业结构,合理利用资源,建立村民监督委员会,以推进生态文明建设和实现可持续发展。

关键词：生态环境评价生态环境保护划定红线保护与发展传统村落大芦村

Effects of droplet size and spray volume parameters on droplet deposition of wheat herbicide application by using UAV

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International Journal of Agricultural and Biological Engineering 2021年第1期14卷 74-81页

作者： Changfeng shan guobin Wang Haihong Wang Yingjie Xie Huizheng Wang Shilong Wang Shengde Chen Yubin Lan College of Agricultural Engineering and Food Science Shandong University of TechnologyShandong Provincial Engineering Technology Research Center for Agricultural Aviation Intelligent EquipmentZibo 255022ShandongChina Corteva Agroscience Technology(shanghai)Co. LtdShanghai 201203China Zibo Agricultural and Rural Service Center Zibo 255022ShandongChina National Center for International Collaboration Research on Precision Agricultural Aviation Pesticides Spraying Technology(NPAAC) Ministry of Science and TechnologyCollege of Electronics EngineeringSouth China Agricultural UniversityGuangzhou 510642China Department of Biological and Agricultural Engineering Texas A&M UniversityCollege StationTX 77845USA

With the characteristic of flexible and precise,unmanned aerial vehicles(UAVs)for low volume applications are increasing substantially and quickly around the ***,little attention has been paid to the study of wheat herbicides with UAV,especially the research on the spray volume and droplet size of the herbicide sprayed by *** objectives of this study were to compare the droplet deposition from a typical commercial UAV under four different spray volumes of 7.5 L/hm2,15.0 L/hm2,22.5 L/hm2,and 30.0 L/hm2 and three different volume median diameter(VMD)of 150μm,200μm,and 300μm during winter wheat weeding *** software was used to analyze droplet deposition parameters including the percentage of spray coverage and the number of droplets in various sampling *** test results showed that the droplet deposition waseffected by each factor *** the spray volume was 7.5 L/hm2,the effect of VMD on the percentage of spray coverage was not ***,these variation rules were changed to smaller droplets with greater coverage when the spray volume higher than 15.0 L/*** all treatments,the number of droplets increased with decreasing VMD or increasing spray *** maximum percentage of spray coverage and the number of droplets that were achieved under the VMD of 150μm and the spray volume of 30.0 L/hm2 were 12.8%and 40.0 droplets/cm2,*** variation coefficients of the percentage of spray coverage and the number of droplets were 29.0%-73.3%and 20.2%-54.1%,*** most uniform deposition was achieved under the spray volume of 15.0 L/hm2and the VMD of 150μ*** results revealed the effect of droplet size and spray volume parameters on droplet deposition,which was useful for guiding farmers on how to use UAVs for weeding in winter wheat fields.

关键词： unmanned aerial vehicle spray volume droplet size droplet deposition

Application of a microseismic monitoring system in deep mining

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Journal of University of Science and Technology Beijing 2007年第1期14卷 6-8页

作者： Chengxiang Yang Zhouquan Luo guobin Hu Xiaoming Liu School of Resources and Safety Engineering Central South University Changsha 410083 China

A microseismic monitoring system was used in the Donggua shan underground copper mine, and its application was introduced. The spacial distribution of the seismic event was monitored effectively during mining with this system. The distribution of the seismic intensity in different time periods and in the different mining districts was obtained via the clustering analysis of the monitored results, and the different intensity concentration districts of seismicity were compartmentalized. The various characteristics and waveforms of different vibrations in the underground mine were revealed with the help of the micro-seismic monitoring system. It was proved that the construction and application of the micro-seismic monitoring system in the mine not only realized the continuous monitoring of seismicity in the deep mine, but also settled an this system.

关键词： microseismicity monitoring event deep mining important foundation for further studies on hazard prediction, based on

水泥稳定再生骨料混合料力学性能和抗冻性能研究

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硅酸盐通报 2024年第2期43卷 774-780页

作者：单社郭海贞魏定邦刘辉阮志琦任国斌甘肃省交通投资管理有限公司兰州730000 甘肃省交通规划勘察设计院股份有限公司兰州730000

为促进再生骨料在半刚性基层中的应用,实现建筑垃圾在公路工程中的资源化利用,本文采用等质量置换法,系统分析了再生骨料掺量(质量分数)为0%、30%、50%、70%、100%时对无机混合料最大干密度、最佳含水率、无侧限抗压强度、抗冻性能的影... 详细信息

为促进再生骨料在半刚性基层中的应用,实现建筑垃圾在公路工程中的资源化利用,本文采用等质量置换法,系统分析了再生骨料掺量(质量分数)为0%、30%、50%、70%、100%时对无机混合料最大干密度、最佳含水率、无侧限抗压强度、抗冻性能的影响,并从微观角度解释了内在影响机理。结果表明:掺入再生骨料降低了无机混合料的最大干密度,增大了最佳含水率;再生骨料可以有效提高无机混合料的无侧限抗压强度和抗冻性能,当再生骨料掺量为70%时提升效果最佳,此时再生骨料无机混合料7、28 d无侧限抗压强度为4.6、6.8 MPa, 28、90 d冻融循环后残留抗压强度比可达82.4%、85.5%。

关键词：再生骨料无侧限抗压强度抗冻性能最大干密度最佳含水率

Construction,identification and biological feature study of human hepatocellular carcinoma cells stably expressing HBeAg

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广西医科大学学报 2017年第12期34卷 1681-1685页

作者： Yuxuan Li shan Huang Kezhi Li guobin Wu Hao Tian Yinnong Zhao Department of Hepatobiliary Surgery Affiliated Cancer Hospital of Guangxi Medical UniversityNanning530021China Graduate School Guangxi Medical UniversityNarnning530021China Department of Research Affiliated Cancer Hospital of Guangxi Medical UniversityNanning530021China

Objective: To construct a HepG2 cell line which stably expressing Hepatitis B e antigen(HBeAg)and investigate the effects of HBeAg on the proliferation,migration and invasion of HepG2 cells. Methods: The lentivirus carrying HBeAg gene was constructed and packaged. HepG2 cells were infected with the lentivirus and screened with puromycin to obtain HepG2 cells which stably expressing HBeAg(HepG2-HBeAg cells).The expression levels of HBeAg mRNA and protein were detected by RT-qPCR and Western blot,respectively. The content of HBeAg secretion in cell supernatant in both HepG2-HBeAg cells and control cells(HepG2-NC cells and HepG2 cells)were detected by IFMA assay. Furthermore,CCK-8 proliferation assay,colony formation assay and transwell migration and invasion assays were conducted to compare the abilities of cell proliferation,migration and invasion,respectively. Results: The expression of HBeAg in the HepG2-HBeAg cell was significantly higher than those in HepG2 cells and HepG2-NC cells. Secreted HBeAg in the supernatant of HepG2-HBeAg cells was 26. 33±2. 13 PEIU/mL but was undetectable in supernatant of control cells. The proliferation,migration and invasion were all significantly lower in HepG2-HBeAg cells compared to control cells(P<0. 01). Conclusion: A HepG2 cell line which stably expressing HBeAg was constructed successfully,and the over-expression of HBeAg could attenuate the proliferation,migration and invasion of hepatocellular carcinoma cells.

关键词： HBeAg hepatocellular carcinoma cells proliferation migration invasion

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基于肺部CT影像的针道识别关键技术研究

天津大学学报（自然科学与工程技术版） 2019年第4期52卷 346-352页

作者：鲍潮姜杉张国彬杨志永天津大学机械工程学院天津300350

为了获得CT引导的肺部近距离放射治疗术中实际针道位置并且辅助术中顺利进行剂量优化步骤,本文提出了一种自动针道重建算法.基于预检验模型参数评估技术、局部随机样本一致性(RANSAC)算法与主成分分析(PCA)法相结合的局部优化算法,所提... 详细信息

为了获得CT引导的肺部近距离放射治疗术中实际针道位置并且辅助术中顺利进行剂量优化步骤,本文提出了一种自动针道重建算法.基于预检验模型参数评估技术、局部随机样本一致性(RANSAC)算法与主成分分析(PCA)法相结合的局部优化算法,所提出的改进的RANSAC算法能够快速、精确、稳定地将针轴提取出来.相较于传统的RANSAC算法,本文首先运用预检验技术加速了传统算法的迭代过程,以便于提高整体拾取效率;其次为了解决传统算法拾取精度较差的问题,先通过局部随机样本一致性算法获得一个近似较优解,而后利用PCA法最小化所有局内点的误差以得到最终的最优解.针尖点的具体位置可以通过搜索针轴方向灰度的变化而确定.此外,同一张切片上的多根针通过连续局内点删除算法能够一次性被分割出来.模拟数据结果表明,改进后算法的分割精度要明显高于传统的RANSAC算法所得的结果,并且随着局内点占比下降拾取时间并没有发生较大的上升.此外,本文的算法也在实际的肺部近距离放射治疗图像中得到了验证,实际病例实验中以手动分割的结果作为算法所得的结果的参照物.实验结果表明,针轴方向和针尖点的平均误差分别被控制在1.4°和0.85 mm以内,且平均每根针的分割时间为0.238 s.

关键词：肺部近距离放射治疗 CT影像随机样本一致性算法针道识别