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Prophenoloxidase from Pieris rapae:gene cloning,activity,and transcription in response to venom/calyx fluid from the endoparasitoid wasp Cotesia glomerata

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第2期12卷 103-115页

作者： Jia-ying ZHU Pu YANG guo-xing wu Key Laboratory of Forest Disaster Warning and Control of Yunnan Province and Key Laboratory of Southwest Mountain Forest Resources Conservation and Utilization of Ministry of Education Southwest Forestry University Kunming 650224 China Research Institute of Resource Insects Chinese Academy of Forestry Kunming 650224 China College of Plant Protection Yunnan Agricultural University Kunming 650201 China

Prophenoloxidase (PPO) plays an important role in melanization, necessary for defense against intruding parasitoids. Parasitoids have evolved to inject maternal virulence factors into the host hemocoel to suppress hemolymph melanization for the successful development of their progeny. In this study, the full-length complementary DNA (cDNA) of a Pieris rapae PPO was cloned. Its cDNA contained a 2 076-base pair (bp) open reading frame (ORF) encoding 691 amino acids (aa). Two putative copper-binding sites, a proteolytic activation site, three conserved hemocyanin domains, and a thiol ester motif were found in the deduced amino acid sequence. According to both multiple alignment and phylogenetic analysis, P. rapae PPO gene cloned here is a member of the lepidopteran PPO-2 family. Injection of Cotesia glomerata venom or calyx fluid resulted in reduction of P. rapae hemolymph phenoloxidase activity, demonstrating the ability to inhibit the host's melanization. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) showed that transcripts of P. rapae PPO-2 in the haemocytes from larvae had not significantly changed following venom injection, suggesting that the regulation of PPO messenger RNA (mRNA) expression by venom was not employed by C. glomerata to cause failure of melanization in parasitized host. While decreased P. rapae PPO-2 gene expression was observed in the haemocytes after calyx fluid injection, no detectable transcriptional change was induced by parasitization, indicating that transcriptional down-regulation of PPO by calyx fluid might play a minor role involved in inhibiting the host's melanization.

关键词： Prophenoloxidase Cloning Venom Calyx fluid Gene expression Parasitoid

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Heat shock protein genes （hsp20, hsp75 and hsp90） from Pieris rapae： Molecular cloning and transcription in response to parasitization by Pteromalus puparum

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Insect Science 2013年第2期20卷 183-193页

作者： Jia-Ying Zhu guo-xing wu Gong-Yin Ye Cui Hu Key Laboratory of Forest Disaster Warning and Control of Yunnan Province Southwest Forestry University Kunming College of Plant Protection Yunnan Agricultural University Kunming Key laboratory of Molecular Biology of Crop Pathology and Insects of Ministry of Agriculture Institute of Insect Sciences Zhejiang University Hangzhou China

Most molecular work on the roles of heat shock proteins （hsps） in host-parasite interaction has focused on vertebrates, rather than invertebrates. Here the full length complementary DNA （cDNA） sequences of three hsp genes （hsp20, hsp75 and hsp90） were amplified from Pieris rapae, and their transcriptional responsiveness to parasitization by the endoparasitic wasp Pteromalus puparum were investigated. The cDNA sequence analysis of hsp20, hsp75 and hsp90 revealed open reading frames of 531, 2328 and 2 157 bp in length, which encode proteins with calculated molecular weights of 19.5, 75.48 and 82.7 kDa, respectively. The comparison of amino acid sequences showed that P rapae hsp20 shared highly divergent homology to that of other insects, while hsp75 and hsp90 showed high homology to their counterparts of other species. The expression analysis indicated that these three genes were influenced in response to parasitization by P. puparum. The hsp20 transcripts in parasitized pupae were higher compared to non- parasitized pupae. The expression of hsp75 and hsp90 were down-regulated following parasitization. The results indicate that hsps are involved in host-parasitoid interactions.

关键词： gene expression heat shock protein parasitization

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High-throughput genotyping of single-nucleotide polymorphisms in ace-1 gene of mosquitoes using MALDI-TOF mass spectrometry

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Insect Science 2013年第2期20卷 167-174页

作者： Yun Mao Feng Tan Shuai-guo Yan guo-xing wu Chuan-Ling Qiao Wen-Xue Zhang Feng Cui College of Life Sciences Henan Normal University Xinxiang Henan Province State Key Laboratory of Integrated Management of Pest Insects & Rodents Institute of Zoology Chinese Academy of Sciences Beijing College of Plant Protection Yunnan Agricultural University Kunming China

Acetylcholinesterase （ACHE） plays a vital role in the nervous system of insects and other animal species and serves as the target for many chemical agents such as organophosphate and carbamate insecticides. The mosquito, Culex pipiens complex, a vector of human disease, has evolved to be resistant to insecticides by a limited number of amino acid substitutions in ACHE1, which is encoded by the ace-1 gene. The aims of this study are to identify single nucleotide polymorphism （SNP） sites in the ace-1 gene of the C. pipiens complex and explore an economical high-throughput method to differentiate the genotypes of these sites in mosquitoes collected in the field. We identified 22 SNP sites in exon regions of the ace-1 gene. Four of them led to non-synonymous mutations, that is, Y163C, G247S, C677S and T682A. We used matrix-assisted laser desorption ionization - time-of-flight mass spectrometry for genotyping at these four sites and another site F416V, which was relevant to insecticide resistance, in 150 mosquitoes collected from 15 field populations. We were able to synchronize analysis of the five SNP sites in each well of a 384-well plate for each individual mosquito, thus decreasing the cost to one-fifth of the routine analysis. Heterozygous genotypes at Y163C and G247S sites were observed in one mosquito. The possible influence of the five SNP sites on the activity or function of the enzyme is discussed based on the predicted tertiary structure of the enzyme.

关键词： acetylcholinesterase genotype insecticide resistance MassArray SNP

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Accumulation of cadmium and its effects on growth, development and hemolymph biochemical compositions in Boettcherisca peregrina larvae （Diptera： Sarcophagidae）

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Insect Science 2006年第1期13卷 31-39页

作者： guo-xing wu GONG-YIN YE CUI HU JIA-AN CHENG Institute of Applied Entomology College of Agriculture and Biotechnology Zhejiang University Hangzhou China

Newly emerged larvae of the fleshfly, Boettcherisca peregrina were exposed to two different CdCl2 concentrations of 100μg/g and 400 mg/g diet fresh weight （DFW）. They were administered in the diets until the end of larval stage. Cd-exposed larvae accumulated significant amounts of Cd and this accumulation increased with the exposure dose and time. The body weights were lightened and lengths of larvae were shortened considerably after Cd exposure, especially at the higher Cd concentration. The total larval duration was also extremely affected due to Cd exposure. The average duration was prolonged significantly by 14 h at the lower Cd concentration, while it was increased by 33.7 h over controls at the higher Cd concentration. A significant decrease in contents of either soluble proteins, total lipids or caloric values in the hemolymph occurred due to Cd exposure throughout the entire tested period but after 120 h of Cd exposure. In contrast, when exposed to Cd with its higher concentration, total sugar contents in the hemolymph were increased strikingly over the whole tested time, except after 96 h of Cd exposure, while they were not apparently altered except after 24 h of Cd exposure at the lower concentration. Thus, it is suggested that Cd exposure shows significant adverse impact on the growth and development, as well as metabolism, in larvae of this fleshfly, depending on its exposure time and dose.

关键词： Boettcherisca peregrina cadmium caloric value growth and development,lipids proteins total sugar

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气相沉积膜层在镁合金表面改性中的应用

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材料工程 2006年第1期34卷 61-65页

作者：吴国松曾小勤郭兴伍姚寿山上海交通大学轻合金精密成型国家工程研究中心上海200030

综述了气相沉积膜层的特性、制备技术的发展及其在镁合金表面改性中的应用,重点介绍了利用PVD和CVD技术在镁合金表面沉积保护性膜层的研究现状,并指出气相沉积膜层是传统电化学镀层的良好替代物,在镁合金表面改性中具有广阔的应用前景。

关键词：气相沉积镁合金耐蚀性耐磨性

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镁/钢异种板自冲铆连接及耐腐蚀性能研究

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机械设计与制造 2023年第1期383卷 116-119页

作者：郭兴伍陈娟李晔李滇上海交通大学材料科学与工程学院上海200240 邦林机械制造(上海)有限公司上海201401

现有的镁/钢件连接技术尚不能完全满足实际应用的技术要求,压铆、锤铆和旋转半空心铆钉自冲铆接技术(SPR)等容易使镁合金件产生开裂;电阻点焊和搅拌摩擦焊等技术连接强度太低,达不到连接强度要求;螺栓连接和拉铆必须预先打孔,存在操作... 详细信息

现有的镁/钢件连接技术尚不能完全满足实际应用的技术要求,压铆、锤铆和旋转半空心铆钉自冲铆接技术(SPR)等容易使镁合金件产生开裂;电阻点焊和搅拌摩擦焊等技术连接强度太低,达不到连接强度要求;螺栓连接和拉铆必须预先打孔,存在操作和装配不方便等问题。研发了一种爆炸驱动的超高速自冲铆接用铆枪和铆接新工艺,铆钉从射钉器的射击口射出的速度大于152m/s,从而实现镁合金板与钢板的实心铆钉自冲铆接,避免镁合金板的开裂,使镁/钢牢固连接,拉剪力F_(m)≈5273.1N。采用此技术铆接的镁/钢板材试样用密封胶对铆钉及连接搭接部位缝隙进行密封后,耐腐蚀实验(GB/T6461-2002)研究表明,中性盐雾测试结果可达1500小时10级,即试样完好,无腐蚀破坏发生。

关键词：镁合金钢爆炸驱动高速自冲铆接电偶腐蚀

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Multiple Tin Compounds Modified Carbon Fibers to Construct Heterogeneous Interfaces for Corrosion Prevention and Electromagnetic Wave Absorption

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Nano-Micro Letters 2025年第1期17卷 507-527页

作者： Zhiqiang guo Di Lan Zirui Jia Zhenguo Gao Xuetao Shi Mukun He Hua guo Guanglei wu Pengfei Yin College of Science Sichuan Agricultural UniversityYa’an 625014People’s Republic of China School of Materials Science and Engineering Hubei University of Automotive TechnologyShiyan 442002People’s Republic of China Institute of Materials for Energy and Environment State Key Laboratory of Bio‑Fibers and Eco‑TextilesCollege of Materials Science and EngineeringQingdao UniversityQingdao 266071People’s Republic of China Shaanxi Key Laboratory of Macromolecular Science and Technology School of Chemistry and Chemical EngineeringNorthwestern Polytechnical UniversityXi’anShaanxi 710072People’s Republic of China

Currently,the demand for electromagnetic wave(EMW)absorbing materials with specific functions and capable of withstanding harsh environments is becoming increasingly ***-component interface engineering is considered an effective means to achieve high-efficiency EMW ***,interface modulation engineering has not been fully discussed and has great potential in the field of EMW *** this study,multi-component tin compound fiber composites based on carbon fiber(CF)substrate were prepared by electrospinning,hydrothermal synthesis,and high-temperature thermal *** utilizing the different properties of different substances,rich heterogeneous interfaces are *** effectively promotes charge transfer and enhances interfacial polarization and conduction *** prepared SnS/SnS_(2)/SnO_(2)/CF composites with abundant heterogeneous interfaces have and exhibit excellent EMW absorption properties at a loading of 50 wt%in epoxy *** minimum reflection loss(RL)is−46.74 dB and the maximum effective absorption bandwidth is 5.28 ***,SnS/SnS_(2)/SnO_(2)/CF epoxy composite coatings exhibited long-term corrosion resistance on Q235 steel ***,this study provides an effective strategy for the design of high-efficiency EMW absorbing materials in complex and harsh environments.

关键词： Electrostatic spinning Component regulation Heterogeneous interfaces Electromagnetic wave absorption Corrosion protection

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Clinical effects of phospholipase D2 in attenuating acute pancreatitis

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World Journal of Gastroenterology 2025年第2期31卷 52-60页

作者： Jin-Wei Niu guo-Chao Zhang wu Ning Hai-Bin Liu Hua Yang Chao-Feng Li Department of General Surgery China-Japan Friendship HospitalBeijing 100029China

BACKGROUND The objective of the current study was to elucidate the clinical mechanism through which phospholipase D2(PLD2)exerted a regulatory effect on neutrophil migra-tion,thereby alleviating the progression of acute *** To elucidate the clinical mechanism through which PLD2 exerted a regulatory effect on neutrophil migration,thereby alleviating the progression of acute *** The study involved 90 patients diagnosed with acute pancreatitis,admitted to our hospital between March 2020 and November 2022.A retrospective analysis was conducted,categorizing patients based on Ranson score severity into mild(n=25),moderate(n=30),and severe(n=35)*** data was collected for each *** blot analysis assessed PLD2 protein expression in patient ***-time reverse transcription polymerase chain reaction was used to evaluate the mRNA expression of chemokine receptors associated with neutrophil *** levels of inflammatory factors in patients were detected using enzyme-linked immunosorbent *** migration tests were conducted to compare migration of neutrophils across groups and analyze the influence of PLD2 on neutrophil *** Overall data analysis did not find significant differences between patient groups(P>0.05).The expression of PLD2 protein in the severe group was lower than that in the moderate and mild groups(P<0.05).The expression level of PLD2 in the moderate group was also lower than that in the mild group(P<0.05).The severity of acute pancreatitis is negatively correlated with PLD2 expression(r=-0.75,P=0.002).The mRNA levels of C-X-C chemokine receptor type 1,C-X-C chemokine receptor type 2,C-C chemokine receptor type 2,and C-C chemokine receptor type 5 in the severe group are significantly higher than those in the moderate and mild groups(P<0.05),and the expression levels in the moderate group are also higher than those in the mild group(P<0.05).The levels of C-reactive protein,tumo

关键词： Phospholipase D2 Neutrophil migration Acute pancreatitis Retrospective analysis Inflammatory response

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深黄被孢霉Δ~6-脂肪酸脱氢酶基因在转基因烟草中的表达

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生物工程学报 2003年第2期19卷 178-184页

作者：李明春刘莉胡国武邢来君南开大学微生物系天津300071

γ 亚麻酸 (GLA)是人体和动物饮食中具有营养作用的重要的多烯不饱和脂肪酸 ,在大多数油料作物种子中不含有GLA ,而只含有其前体物亚油酸 ,只有少数油料植物种子中含有GLA ,如夜来香 (Oenotheraspp) ,琉璃苣(Boragoofficinalis)等。Δ6 ... 详细信息

γ 亚麻酸 (GLA)是人体和动物饮食中具有营养作用的重要的多烯不饱和脂肪酸 ,在大多数油料作物种子中不含有GLA ,而只含有其前体物亚油酸 ,只有少数油料植物种子中含有GLA ,如夜来香 (Oenotheraspp) ,琉璃苣(Boragoofficinalis)等。Δ6 脂肪酸脱氢酶可将亚油酸转化为γ 亚麻酸 ,为了能够在传统的油料作物种子中产生GLA ,我们将从深黄被孢霉中克隆的Δ6 脂肪酸脱氢酶基因 ,与植物表达载体pGA6 43连接 ,构建了重组质粒pGAMICL6 ,将其通过农杆菌介导法 ,导入模式植物烟草中。经PCR和Southern杂交分析表明该基因已导入并整合到烟草的基因组中 ,Northern杂交结果表明该基因在转基因烟草的mRNA水平上获得表达。对转基因植株进行脂肪酸分析 ,结果显示 ,GLA和十八碳四烯酸 (OTA)分别占总脂肪酸含量的 19 7%和 3 5 %。

关键词：烟草脂肪酸脱氢酶基因亚麻酸转基因植株深黄被孢霉

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Resting-state brain network remodeling after different nerve reconstruction surgeries:a functional magnetic resonance imaging study in brachial plexus injury rats

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Neural Regeneration Research 2025年第5期20卷 1495-1504页

作者： Yunting Xiang Xiangxin xing Xuyun Hua Yuwen Zhang Xin Xue Jiajia wu Mouxiong Zheng He Wang Jianguang Xu School of Rehabilitation Science Shanghai University of Traditional Chinese MedicineShanghaiChina Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation Ministry of EducationShanghaiChina Department of Rehabilitation Medicine Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghaiChina Department of Traumatology and Orthopedics Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghaiChina Institute of Science and Technology for Brain-Inspired Intelligence Fudan UniversityShanghaiChina Key Laboratory of Computational Neuroscience and Brain-Inspired Intelligence(Fudan University) Ministry of EducationShanghaiChina Human Phenome Institute Fudan UniversityShanghaiChina

Distinct brain remodeling has been found after different nerve reconstruction strategies,including motor representation of the affected ***,differences among reconstruction strategies at the brain network level have not been *** study aimed to explore intranetwork changes related to altered peripheral neural pathways after different nerve reconstruction surgeries,including nerve repair,endto-end nerve transfer,and end-to-side nerve ***–Dawley rats underwent complete left brachial plexus transection and were divided into four equal groups of eight:no nerve repair,grafted nerve repair,phrenic nerve end-to-end transfer,and end-to-side transfer with a graft sutured to the anterior upper ***-state brain functional magnetic resonance imaging was obtained 7 months after *** independent component analysis algorithm was utilized to identify group-level network components of interest and extract resting-state functional connectivity values of each voxel within the *** in intra-network resting-state functional connectivity were compared among the *** muscle reinnervation was assessed by behavioral observation(elbow flexion)and *** results showed that alterations in the sensorimotor and interoception networks were mostly related to changes in the peripheral neural *** repair was related to enhanced connectivity within the sensorimotor network,while end-to-side nerve transfer might be more beneficial for restoring control over the affected limb by the original motor *** thalamic-cortical pathway was enhanced within the interoception network after nerve repair and end-to-end nerve *** areas related to cognition and emotion were enhanced after end-to-side nerve *** study revealed important brain networks related to different nerve *** networks may be potential targets for enhancing motor recovery.

关键词： brain functional networks end-to-end nerve transfer end-to-side nerve transfer independent component analysis nerve repair peripheral plexus injury resting-state functional connectivity

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