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Military Medical Research 2024年第5期11卷 747-784页

作者： Lin-Sheng Li Ling Yang Li Zhuang zhao-Yang Ye wei-guo zhao Wen-Ping Gong Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment Senior Department of Tuberculosisthe Eighth Medical Center of PLA General HospitalBeijing100091China Hebei North University Zhangjiakou075000HebeiChina Senior Department of Respiratory and Critical Care Medicine the Eighth Medical Center of PLA General HospitalBeijing100091China

Latent tuberculosis infection(LTBI)has become a major source of active tuberculosis(ATB).Although the tuberculin skin test and interferon-gamma release assay can be used to diagnose LTBI,these methods can only differentiate infected individuals from healthy ones but cannot discriminate between LTBI and ***,the diagnosis of LTBI faces many challenges,such as the lack of effective biomarkers from Mycobacterium tuberculosis(MTB)for distinguishing LTBI,the low diagnostic efficacy of biomarkers derived from the human host,and the absence of a gold standard to differentiate between LTBI and *** culture,as the gold standard for diagnosing tuberculosis,is time-consuming and cannot distinguish between ATB and *** this article,we review the pathogenesis of MTB and the immune mechanisms of the host in LTBI,including the innate and adaptive immune responses,multiple immune evasion mechanisms of MTB,and epigenetic *** on this knowledge,we summarize the current status and challenges in diagnosing LTBI and present the application of machine learning(ML)in LTBI diagnosis,as well as the advantages and limitations of ML in this ***,we discuss the future development directions of ML applied to LTBI diagnosis.

关键词： Tuberculosis(TB) Latent tuberculosis infection(LTBI) Machine learning(ML) Biomarkers Differential diagnosis

Antiosteoporosis and bone protective effect of alpinumisoflavone in steroid-induced osteoporosis rats via alteration of apoptosis,inflammatory and RANK/RANKL/OPG signaling pathway

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Asian Pacific Journal of Tropical Biomedicine 2024年第10期14卷 436-447页

作者： Jie Lian Jun-Long Qu guo-wei zhao Xu-Biao Ji Department of Orthopaedics Luzhou Traditional Chinese Medicine HospitalLuzhouSichuan646000China Department of Orthopedic Rehabilitation Shengli Oilfield Central HospitalDongyingShandong257000China Department of Spinal Surgery Changzhou Wujin People's HospitalWujin Hospital Affiliated with Jiangsu UniversityChangzhouJiangsu213000China

Objective:To estimate the bone protective effect of alpinumisoflavone,a natural prenylated isoflavonoid,against glucocorticoid-induced osteoporosis in ***:Male Wistar rats received intramuscular administration of dexamethasone(4 mg/mL)at a dose level of 7 mg/kg for 5 weeks,and then alpinumisoflavone(5,10,and 15 mg/kg)and alendronate(2 mg/kg)from 2 *** body weight and organ weight(femoral,vagina,and uterus)were *** analysis,bone turnover markers,bone parameters,oxidative stress parameters,and inflammatory cytokines were *** expressions of related genes were also ***:Alpinumisoflavone remarkably boosted body weight and organ weight(ureters and vagina),improved microCT analysis parameters,and boosted levels of bone ***,alpinumisoflavone considerably(P<0.001)restored the level of bone turnover markers and oxidative stress parameters,remarkably suppressed the level of cytokines such as interleukin-1β,interleukin-6,tumor necrosis factor-α,and increased transforming growth factor-βand insulin-like growth *** also significantly restored the osteoprotegerin(OPG,RANKL,and OPG/RANKL ratio)levels and the mRNA expression of Caspase(3,6,7,9),BMPs,OPN,ALP,RUNX2,and ***:The current result suggests the bone protective effect of alpinumisoflavone against glucocorticoid-induced osteoporosis in rats.

关键词： Osteoporosis Alpinumisoflavone Bone parameters Inflammation Oxidative stress

Enzymatic Synthesis of Agmatine by Immobilized Escherichia coli Cells with Arginine Decarboxylase Activity

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Chemical Research in Chinese Universities 2011年第6期27卷 992-995页

作者： ZHANG wei-guo zhao Gen-hai LIU Jun-zhong LIU Qian JIAO Qing-cai State Key Laboratory of Pharmaceutical Biotechnology School of Life Science Nanjing University Nanjing 210093 P. R. China

A new method for the enzymatic synthesis of agmatine by immobilized Escherichia coli cells with arginine decarboxylase（ADC） activity was established and a series of optimal reaction conditions was set down. The arginine decarboxylase showed the maximum activity when the pyridoxal phosphate（PLP） concentration was 50 mmol/L, pH=7 and 45 °C. The arginine decarboxylase exhibited the maximum production efficiency when the substrate concentration was 100 mmol/L and the reaction time was 15 h. It was also observed that the appropriate concentration of Mg2＋, especially at 0.5 mmol/L promoted the arginine decarboxylase activity; Mn2＋ had little effect on the arginine decarboxylase activity. The inhibition of Cu2＋ and Zn2＋ to the arginine decarboxylase activity was significant. The immobilized cells were continuously used 6 times and the average conversion rate during the six-time usage was 55.6%. The immobilized cells exhibited favourable operational stability. After optimization, the maximally cumulative amount of agmatine could be up to 20 g/L. In addition, this method can also catalyze D,L-arginine to agmatine, leaving the pure optically D-arginine simultaneously. The method has a very important guiding significance to the enzymatic preparation of agmatine.

关键词： Arginine decarboxylase Agmatine Enzymatic resolution Immobilized cell

Lysine-specific Demethylase 1 Represses THP-1 Monocyte-to-macrophage Differentiation

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Chinese Medical Sciences Journal 2013年第2期28卷 82-87页

作者： Rui-feng Yang guo-wei zhao Shu-ting Liang Hou-zao Chen De-pei Liu National Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences & Peking Union Medical College

Objective To investigate the role of lysine-specific demethylase 1 （LSD1） in the process of THP-1 monocyte-to-macrophage differentiation. Methods Quantitative reverse transcription-polymerase chain reaction （qRT-PCR） and Western blotting were performed to analyze the expression of LSD1 and interleukin-6 （IL-6） in THP-1 monocytes and THP-l-derived macrophages. Chromatin immunoprecipitation （ChiP） assay was applied to detect the occupancy of LSD1 and H3K4 methylation at IL-6 promoter during THP-1 monocyte-to-macrophage differentiation. IL-6 mRNA level and H3K4 methylation at IL-6 promoter were analyzed using qRT-PCR and ChiP assay in LSD 1 -knockdown THP- 1 cells treated with 12-O-tetradecanoylphorbol- 13-acetate （TPA） for 0 4, 8, 12, and 24 hours. Fluorescence activated flow cytometry was performed to reveal the percentage of macrophages differentiated from THP- 1 monocytes. Results The expression of LSD1 reduced during THP-1 monocyte-to-macrophage differentiation （P〈0.01）. LSD1 occupancy decreased and H3K4 methylation increased at IL-6 promoter during the differentiation. With knockdown of LSD1, H3K4 methylation at IL-6 promoter was found increased after TPA treatment at different times points （all P〈0.05, except 24 hours）. The percentage of macrophages increased significantly in theTHP-I cells with LSD1 knockdown （P〈0.05）. Conclusions LSD1 is repressed during the monocyte-to-macrophage differentiation of THP-1 cells. Suppression of LSD 1-mediated H3K4 demethylation may be required for THP-1 monocyte-to-macrophage differentiation.

关键词： lysine-specific demethylase 1 interleukin-6 H3K4 methylation monocyte-to-macrophage differentiation

Hard Magnetic Properties of Sm-Fe-C Based Alloys Prepared by Mechanical Alloying

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Journal of Materials Science & Technology 2000年第2期16卷 125-126页

作者： Dianyu GENG, Zhidong ZHANG, Baozhi CUI, Zhijun guo, wei LIU and Xinguo zhao (International Center for Materials Physics, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110015, China) International Center for Materials Physics Institute of Metal Research Chinese Academy of Sciences Shenyang 110015 China

The structure and magnetic properties of SmyFe100-1.5yC0.5y(y=8～20) alloys prepared by mechanical alloying (MA) from Sm, Fe and graphite have been investigated systematically. In order to improve hard magnetic properties of the alloys prepared by mechanical alloying, a new method consisting of re-milling and re-annealing was developed. After being re-milled and re-annealed, the Curie temperature TC of the Sm-Fe-C alloys changes. The TC of 2:17 phase increases, whereas the TC of 2:14:1 phase decreases. After being re-annealed at low temperatures, the grain sizes of hard phases are smaller than those in the alloys annealed at high temperatures. The effects of Co or Ti substitution for Fe are studied.

关键词： Fe Hard Magnetic Properties of Sm-Fe-C Based Alloys Prepared by Mechanical Alloying Sm

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李清照词中的情与景新论

广西民族大学学报（哲学社会科学版） 2008年第4期30卷 161-164页

作者：韦国兆南宁师范高等专科学校广西龙州532400

李清照是中国南宋时期著名的女词人,被后人誉为词中皇后。李清照词是从其切身的生活感受出发,进行艰苦的艺术构思,抒发了自己的真情实感。借景抒情、寓情于景和情景融合是李清照词用以抒发情感的主要方式,由此可以感悟作者的心与志。

关键词：李清照词情与景情景融合

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某型中口径舰炮改进方法

舰船科学技术 2015年第3期37卷 134-138页

作者：郭昭蔚中国船舶重工集团公司第七一三研究所河南郑州450015

为提高某型中口径舰炮的防空反导能力,对该型舰炮的作战效能开展研究。针对以提高射速为目的的多管舰炮和以提高舰炮系统精度为目标的新型单管舰炮2种方案进行对比,按高炮的点射过程构建仿真计算模型。对2种方案分别进行毁歼概率和单发... 详细信息

为提高某型中口径舰炮的防空反导能力,对该型舰炮的作战效能开展研究。针对以提高射速为目的的多管舰炮和以提高舰炮系统精度为目标的新型单管舰炮2种方案进行对比,按高炮的点射过程构建仿真计算模型。对2种方案分别进行毁歼概率和单发命中概率的分析计算,证明通过提高舰炮系统精度实现舰炮提高作战效能的方案更加可行,并提出合理的舰炮系统精度改进目标。

关键词：舰炮毁歼概率仿真计算系统精度

Mechanism underlying bergapten-mediated regulation of vincristine transport in MDCK-MDR1 cells

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Chinese Herbal Medicines 2018年第3期10卷 255-262页

作者： Xin-li Liang Tao Tang guo-wei zhao wei Dong Xue-jing Guan Zheng-gen Liao Ming Yang Key Laboratory of Modern Preparation of TCM Ministry of EducationJiangxi University of Traditional Chinese Medicine

Objectives: To investigate the effects of bergapten of Angelicae Dahuricae Radix on the transport of vincristine and its possible ***: The apparent permeability coefficient(Papp) for the transport of vincristine through the membrane of MDCK-MDR1 cells was used as an indicator of the effect of bergapten on vincristine *** docking was employed to predict the binding force between bergapten and P-glycoprotein(Pgp). The effects of bergapten on P-gp function and P-gp ATPase activity were determined by rhodamine123(Rho123) accumulation and activity analysis, respectively. 1,6-Diphenyl-1,3,5-hexatriene(DPH) was used to study the effects of bergapten on membrane fluidity, and Western blotting and quantitative realtime PCR assays were performed to analyze the effect of bergapten on the protein and mRNA expression of P-gp, respectively. These experiments clarified the effects of bergapten on the transport of vincristine and allowed exploration of the possible mechanism underlying the effects of ***: The results showed that bergapten could inhibit the transport of vincristine in MDCK-MDR1 cells,and the binding force between bergapten and P-gp was weaker. Bergapten could reduce the accumulation of Rh123 in MDCK-MDR1 cells, increase the membrane fluidity, and upregulate P-gp protein and mRNA expression but it had no effect on P-gp ATPase ***: Overall, we concluded that the possible mechanism through which bergapten inhibits vincristine transport was related to the bergapten-mediated upregulation of P-gp protein and mRNA expression, membrane fluidity or P-gp enzyme activity.

关键词： bergapten MDCK-MDR1 transport vinvristine

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黄海绿潮分布年际变化特征分析

海洋学报 2016年第12期38卷 36-45页

作者：郭伟赵亮李秀梅天津科技大学海洋与环境学院天津300457

2008—2015年，连续8年在黄海海域暴发大规模绿潮，但因暴发时间、规模及漂移路径的不同，对沿海地区造成的环境影响和经济损失大不相同。本文利用 EOS／MODIS 卫星的多通道资料，采用 NDVI算法获取绿潮信息，给出了2007年以来5—8月所... 详细信息

2008—2015年，连续8年在黄海海域暴发大规模绿潮，但因暴发时间、规模及漂移路径的不同，对沿海地区造成的环境影响和经济损失大不相同。本文利用 EOS／MODIS 卫星的多通道资料，采用 NDVI算法获取绿潮信息，给出了2007年以来5—8月所有无云或少云晴朗天气下黄海海面绿潮的分布情况。黄海绿潮覆盖面积变化每年呈现单峰值分布，MODIS 卫星在5月中旬至6月初首次发现绿潮，随后30～40 d 内达到覆盖面积峰值，7月快速消退，8月上旬完全消失。2007年绿潮出现首年覆盖面积极低，发现绿潮时面积均未超过50 km2；2008年和2009年绿潮覆盖面积峰值分别为3110 km2和4075 km2，自此绿潮灾害成为新的海洋环境事件；2010—2012年绿潮暴发规模异常低值，各年覆盖面积峰值均未超过1800 km2，但从2013年开始绿潮规模逐渐攀升，到2015年绿潮覆盖面积峰值达5629 km2，持续可达98 d，覆盖面积和持续时间为历年之最。绿潮漂移路径可归纳分为3类：2008年和2011年绿潮主体先北向漂移越过34°30′N 后，西北向垂直于岸线漂移，主要对连云港、日照和青岛造成较大影响；2009年和2012年绿潮主体先北向漂移越过35°N 后，东北向平行于岸线漂移，故只有少许绿潮上岸；2010年、2013—2015年绿潮主体北向漂移至近岸后东北向沿岸漂移，对日照、青岛和荣成沿海造成大面积影响。所有年份绿潮影响范围均限于南黄海内，东侧边界最远未越过124．2°E。

关键词：绿潮覆盖面积漂移路径年际变化黄海

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基于PLC实现的火电厂小汽机保护控制系统

热能动力工程 2006年第6期21卷 561-564,581页

作者：李大中倪玮强李锦贤郭召伟华北电力大学自动化系河北保定071003 大唐许昌龙岗发电有限责任公司河南禹州461690

针对300MW机组给水泵小汽机保护系统采用PLC(可编程序控制器)设计了新的控制系统,替代了原继电器控制逻辑。该系统采用双CPU冗余热备和双电源供电设计,硬件结构简单,控制逻辑由梯形图软件编程实现,替代了原继电器控制逻辑,大大提高了系... 详细信息

针对300MW机组给水泵小汽机保护系统采用PLC(可编程序控制器)设计了新的控制系统,替代了原继电器控制逻辑。该系统采用双CPU冗余热备和双电源供电设计,硬件结构简单,控制逻辑由梯形图软件编程实现,替代了原继电器控制逻辑,大大提高了系统的安全可靠性,同时控制逻辑软件中新增设计了小汽机事故跳闸首出记忆功能,为系统的事故分析提供了依据。现场投运表明,该系统运行稳定,符合设计要求。

关键词：火电厂小汽机保护 PLC控制