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P2X7/P2X4 Receptors Mediate Proliferation and Migration of Retinal Microglia in Experimental Glaucoma in Mice

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Neuroscience Bulletin 2022年第8期38卷 901-915页

作者： Meng-Xi Xu guo-li zhao Xin Hu Han Zhou Shu-Ying li Fang li Yanying Miao Bo Lei Zhongfeng Wang State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain ScienceFudan UniversityShanghai200032China Institute of Neuroscience and Third Affiliated Hospital Henan Provincial People’s HospitalHenan Eye InstituteHenan Eye HospitalPeople’s Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhou450003China

Microglia are involved in the inflammatory response and retinal ganglion cell damage in ***,we investigated how microglia proliferate and migrate in a mouse model of chronic ocular hypertension(COH).In COH retinas,the microglial proliferation that occurred was inhibited by the P2X7 receptor(P2X7R)blocker BBG or P2X7R knockout,but not by the P2X4R blocker *** of primary cultured microglia with BzATP,a P2X7R agonist,mimicked the effects of cell proliferation and migration in COH retinas through the intracellular MEK/ERK signaling *** migration assays showed that the P2X4R agonist CTP induced microglial migration,which was completely blocked by *** vivo and in vitro experiments demonstrated that ATP,released from activated Müller cells through connexin43 hemichannels,acted on P2X7R to induce microglial proliferation,and acted on P2X4R/P2X7R(mainly P2X4R)to induce microglial *** results suggest that inhibiting the interaction of Müller cells and microglia may attenuate microglial proliferation and migration in glaucoma.

关键词： Glaucoma Chronic ocular hypertension Retinal microglia Proliferation Migration P2X7R/P2X4R Müller cells

L-and T-type Ca^(2+)channels dichotomously contribute to retinal ganglion cell injury in experimental glaucoma

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Neural Regeneration Research 2023年第7期18卷 1570-1577页

作者： Hong-Ning Wang Wen-Jing Qian guo-li zhao Fang li Yan-Ying Miao Bo Lei Xing-Huai Sun Zhong-Feng Wang State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain ScienceFudan UniversityShanghaiChina Institutes of Neuroscience and Third Affiliated Hospital Henan Provincial People’s HospitalHenan Eye InstituteHenan Eye HospitalPeople’s Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina Eye Institute and Department of Ophthalmology Eye&ENT HospitalNHC Key Laboratory of MyopiaShanghai Key Laboratory of Visual Impairment and RestorationFudan UniversityShanghaiChina

Retinal ganglion cell apoptotic death is the main pathological characteristic of glaucoma,which is the leading cause of irreversible *** of Ca^(2+)homeostasis plays an important role in ***-gated Ca^(2+)channel blockers have been shown to improve vision in patients with ***,whether and how voltage-gated Ca^(2+)channels are involved in retinal ganglion cell apoptotic death are largely *** this study,we found that total Ca^(2+)current densities in retinal ganglion cells were reduced in a rat model of chronic ocular hypertension experimental glaucoma,as determined by whole-cell patch-clamp electrophysiological *** analysis showed that L-type Ca^(2+)currents were downregulated while T-type Ca^(2+)currents were upregulated at the later stage of *** blot assay and immunofluorescence experiments confirmed that expression of the Ca_(V)1.2 subunit of L-type Ca^(2+)channels was reduced and expression of the Ca_(V)3.3 subunit of T-type Ca^(2+)channels was increased in retinas of the chronic ocular hypertension *** tumor necrosis factor-α,an important inflammatory factor,inhibited the L-type Ca^(2+)current of isolated retinal ganglion cells from control rats and enhanced the T-type Ca^(2+)*** changes were blocked by the tumor necrosis factor-αinhibitor XPro1595,indicating that both types of Ca^(2+)currents may be mediated by soluble tumor necrosis factor-α.The intracellular mitogen-activated protein kinase/extracellular signal-regulated kinase pathway and nuclear factor kappa-B signaling pathway mediate the effects of tumor necrosis factor-α.TUNEL assays revealed that mibefradil,a T-type calcium channel blocker,reduced the number of apoptotic retinal ganglion cells in the rat model of chronic ocular *** results suggest that T-type Ca^(2+)channels are involved in disrupted Ca^(2+)homeostasis and apoptosis of retinal ganglion cells in glaucoma,and application of T-type

关键词： apoptosis CaV1.2 CaV3.3 chronic ocular hypertension extracellular signal-regulated kinase mitogen-activated protein kinase nuclear factor-kappa B patch-clamp retina tumor necrosis factor-α

Rac1 Modulates Excitatory Synaptic Transmission in Mouse Retinal Ganglion Cells

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Neuroscience Bulletin 2019年第4期35卷 673-687页

作者： ling-Zhu li Ning Yin Xue-Yan li Yanying Miao Shuo Cheng Fang li guo-li zhao Shu-Min Zhong Xin Wang Xiong-li Yang Zhongfeng Wang Department of Neurology Institutes of Brain Science State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Zhongshan Hospital Fudan University

Ras-related C3 botulinum toxin substrate 1(Racl),a member of the Rho GTPase family which plays important roles in dendritic spine morphology and plasticity,is a key regulator of cytoskeletal reorganization in dendrites and ***,we investigated whether and how Racl modulates synaptic transmission in mouse retinal ganglion cells(RGCs)using selective conditional knockout of Racl(Racl-cKO).Racl-cKO significantly reduced the frequency of AMPA receptor-mediated miniature excitatory postsynaptic currents,while glycine/GABA_A receptor-mediated miniature inhibitory postsynaptic currents were not *** the total GluA1 protein level was increased in Racl-cKO mice,its expression in the membrane component was *** did not affect spine-like branch density in single dendrites,but significantly reduced the dendritic complexity,which resulted in a decrease in the total number of dendritic spine-like *** results suggest that Racl selectively affects excitatory synaptic transmission in RGCs by modulating dendritic complexity.

关键词： Rac1 Retinal ganglion cell Excitatory synaptic transmission Dendrite Dendritic spine

Imaging findings and transcatheter arterial chemoembolization of hepatic malignancy with right atrial embolus in 46 patients

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World Journal of Gastroenterology 2008年第22期14卷 3563-3568页

作者： Hong-Yan Cheng Xiao-Yan Wang guo-li zhao Dong Chen Department of Radiology Eastern Hepatobiliary Surgery Hospital 225 Changhai Road Shanghai 200438 China

AIM: To analyze the imaging findings of hepatic malignancy with right atrial (RA) embolus. METHODS: Forty-six patients with an embolus in the RA were diagnosed, including 44 patients with hepatocellular carcinoma (HCC), 1 patient with cholangiocellular carcinoma and 1 patient with hepatic carcinoma metastasis. The diagnosis was confirmed by clinical examination, serum α-fetoprotein and imaging. Seventeen patients underwent transcatheter arterial chemoembolization (TACE). RESULTS: On enhancement computer tomography (CT) or magnetic resonance (MR) imaging, a nodular filling defect in the RA could be easily found, with a slight enhancement in the arterial phase. The coronal images of CT or MR showed the extent of lesion. lipiodol entered the embolus after TACE, hence reducing the speed of embolus growth. There was a survival benefit for patients receiving anticancer treatment. CONCLUSION: Patients with HCC, showing a filling defect of the inferior vena cava (IVC), hepatic vein (HV) and RA on images, can be diagnosed with RA embolus. Encroachment of the RA is very rare in patients with hepatic malignancies. Furthermore, a prolongation of survival time is found in those patients who underwent TACE.

关键词： Carcinoma liver Neoplasm Metastasis Embolus Right atrium Computed tomography X-Ray Magnetic resonance

Clinical diagnosis,treatment,and medical identification of specific pulmonary infection in naval pilots:Four case reports

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World Journal of Clinical Cases 2022年第16期10卷 5487-5494页

作者： Jia Zeng guo-li zhao Jia-Cheng Yi Dan-Dan liu Yan-Qing Jiang Xiang Lu Yan-Bing liu Fei Xue Jie Dong Department of Aviation Disease Naval Medical Center of PLAShanghai 200052China Department of Radiology Naval Medical Center of PLAShanghai 200052China Department of Clinical Medicine School of Basic MedicineHebei Medical UniversityShijiazhuang 050017Hebei ProvinceChina Department of Respiratory Medicine Changhai HospitalNaval Military Medical UniversityShanghai 200081China

BACKGROUND Specific pulmonary infection could seriously threaten the health of pilots and their *** consequences are *** investigated the clinical diagnosis,treatment,and medical identification of specific pulmonary infections in naval *** SUMMARY We analyzed the medical waiver and clinical data of four pilots with specific pulmonary infections,who had accepted treatment at the Naval Medical Center of Chinese People’s liberation Army between January 2020 and November 2021,including three cases of tuberculosis and one of cryptococcal *** cases underwent a series of comprehensive treatment *** cases successfully obtained medical waiver for flight after being cured,while one was grounded after reaching the maximum flight life after being *** Chest computed tomography scanning should be used instead of chest radiography in pilots’physical *** pilots with specific pulmonary infection can be cured and return to flight.

关键词： Cryptococcal pneumonia Tuberculosis Diagnosis Treatment Medical identification Pilot Pulmonary infection Case report

Effect of Helicobacterpyloriinfection on gastric mucosal pathologic change and level of nitric oxide and nitric oxide synthase

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World Journal of Gastroenterology 2005年第32期11卷 5029-5031页

作者： Yong-Fu Wang Chun-lin guo li-Zhen zhao guo-An Yang Peng Chen Hong-Kun Wang First Hospital Baotou Medical College University of Science and Technology of Inner Mongolia Baotou 014010 Inner Mongolia China

AIM： To investigate the level of nitric oxide （NO） and nitrous oxide synthase （NOS） enzyme and its effect on gastric mucosal pathologic change in patients infected with Helicobacter pylori （H pylori）, and to study the pathogenic mechanism of H pylori. METHODS： The mucosal tissues of gastric antrum were taken by endoscopy, then their pathology, H pylori and anti-CagA-IgG were determined. Fifty H pylori positive cases and 35 H pylori negative cases were randomly chosen. Serum level of NO and NOS was detected. RESULTS： One hundred and seven cases （71.33%） were anti-CagA-IgG positive in 150 H pyloripositive cases. The positive rate was higher especially in those with preneoplastic diseases, such as atrophy, intestinal metaplasia and dysplasia. The level of NO and NOS in positive group was higher than that in negative group, and apparently lower in active gastritis than in pre-neoplastic diseases such as atrophy, intestinal metaplasia and dysplasia. CONCLUSION： H pylori is closely related with chronic gastric diseases, and type I H pylori may be the real factor for H pylori-related gastric diseases. Infection with H pylori can induce elevation of NOS, which produces NO.

关键词： Helicobacter pylorr Nitric oxide Nitric oxide synthase Gastric mucosa Pathology

微尺度振荡Couette流的格子Boltzmann模拟

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物理学报 2014年第21期63卷 239-247页

作者：陶实王亮郭照立华中科技大学煤燃烧国家重点实验室武汉430074 北京计算科学研究中心北京100084

采用有效多松弛时间-格子Boltzmann方法(Effective MRT-LBM)数值模拟了微尺度条件下的振荡Couette和Poiseuille流动.在微流动LBM中引入Knudsen边界层模型,对松弛时间进行修正.模拟时平板或外力以正弦周期振动,Couette流中考虑了单平板... 详细信息

采用有效多松弛时间-格子Boltzmann方法(Effective MRT-LBM)数值模拟了微尺度条件下的振荡Couette和Poiseuille流动.在微流动LBM中引入Knudsen边界层模型,对松弛时间进行修正.模拟时平板或外力以正弦周期振动,Couette流中考虑了单平板振动、上下板同相振动这两类情况.研究结果表明,修正后的MRT-LBM模型能有效用于这类非平衡的微尺度流动模拟;对于Couette流,随着Kn数的增大,壁面滑移效应变得越明显.St越大,板间速度剖面的非线性特性越剧烈;两板同相振荡时,若Kn,St均较小,板间流体受到平板拖动剪切的影响很小,板间速度几乎重叠在一起;在振荡Poiseuille流动中,St数增大到一定值时,相位滞后现象减弱;相对于Kn数,St数对振荡Couette和Poiseuille流中不同位置处速度相位差的产生有较大影响.

关键词：格子Boltzmann方法有效MRT模型 Knudsen层振荡流

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考虑热对流的椭圆颗粒沉降规律的研究

工程热物理学报 2017年第5期38卷 1017-1023页

作者：胡俊杰郭照立华中科技大学能源与动力工程学院煤燃烧国家重点实验室武汉430074

基于双分布格子波尔兹曼模型研究了包含热对流的二维椭圆颗粒在竖直管道内的沉降问题,分析了热对流对沉降规律的影响。根据椭圆颗粒运动行为的不同,划分了四个格拉晓夫数区间,即沿管道中心线做周期性振荡、偏离管道中心线稳定沉降、沿... 详细信息

基于双分布格子波尔兹曼模型研究了包含热对流的二维椭圆颗粒在竖直管道内的沉降问题,分析了热对流对沉降规律的影响。根据椭圆颗粒运动行为的不同,划分了四个格拉晓夫数区间,即沿管道中心线做周期性振荡、偏离管道中心线稳定沉降、沿管道中心线做不规则周期性振荡和偏离管道中心线做周期性振荡。在颗粒沉降过程中,强制对流、自然对流和壁面效应三者相互竞争,共同决定颗粒的运动行为。

关键词：颗粒沉降热对流椭圆颗粒格子波尔兹曼方法

Effects of insulin, insulin-like growth factor-I and -II on proliferation and intracellular signaling in endometrial carcinoma cells with different expression levels of insulin receptor isoform A

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Chinese Medical Journal 2013年第8期126卷 1560-1566页

作者： WANG Chun-fang ZHANG guo zhao li-jun li Xiao-ping QI Wen-juan WANG Jian-liu WEI li-hui Department of Obstetrics and Gynecology Peking UniversityPeople's Hospital Beijing 100044 China

Background Hyperinsulinemia, insulin-like growth factor （IGF）-I and -Ⅱ （IGF-Ⅱ） are associated with increased risk of endometrial carcinoma. Insulin receptor isoform A （IR-A） is more frequently expressed in endombtrial carcinoma than in normal endometrial tissues. To better understand their roles in endometrial carcinoma, we investigated the effects of insulin, IGF-I, and IGF-II in endometrial carcinomas cells with different IR-A expression levels. Methods To explore the role of IR-A in mediating the activity of IGF-I, IGF-II, and insulin, we investigate the cellular proliferation of endometrial carcinoma cell lines RL95-2 and RL95-2-1R-A by MTS assays. Then we examined the protein kinase Akt phosphorylation and extracellular signal-regulated kinase （ERK） 1/2 phosphorylation in both cell lines by Western blotting. The effect of IGF-II and AG1024 on cell cycle progression and apoptosis was assessed by fiowcytometry. To examine whether the effects of IGFs were mediated by IR-A, we blocked IGF-I receptor （IGF-IR） in both cell lines using AG1024, an IGF-IR-specific inhibitor. Results IGF-I and IGF-II significantly enhanced proliferation of both cell lines （P 〈0.05）. By contrast, insulin significantly increased proliferation of RL95-2-1R-A cells only （P 〈0.05）. IGF-I and IGF-II significantly increased pAkt levels in RL95-2 cells and pERK1/2 levels in RL95-2-1R-A cells （all, P 〈0.05）. Insulin increased pERK1/2 levels in RL95-2-1R-A cells only （P 〈0.05）. LY294002 and PD98059 inhibited the specific signaling activities and cellular proliferation. After AG1024 pretreatment, neither IGF-I nor IGF-II affected pAkt levels in RL95-2 cells. IGF-II, but not IGF-I, increased pERK1/2 levels in RL95-2-1R-A cells. After AG1024 pretreatment, the proliferation rate and DNA content corresponding to the S phase increased and apoptosis decreased significantly in IGF-II-treated RL95-2-1R-A cells only （P 〈0.05）. Conclusions The proliferation effect of insulin is mediated by IR-A. When IR-A do

关键词： endometrial carcinoma, insulin receptor isoform A insulin, insulin-like growth factor-I insulin-like growth factor-Ⅱ