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An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica

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Journal of Oceanology and Limnology 2024年第1期42卷 216-225页

作者： Zhongyi zhang Hang LIU Xiaohui PAN Yanan ZONG Leili feng Lixian LIU Li guo Guanpin YANG College of Marine Life Sciences Ocean University of ChinaQingdao 266003China Institutes of Evolution and Marine Biodiversity Ocean University of ChinaQingdao 266003China Key Laboratory of Marine Genetics and Breeding of Ministry of Education Ocean University of ChinaQingdao 266003China

Insertional mutation,phenotypic evaluation,and mutated gene cloning are widely used to clone genes from *** genes can be integrated into the genome during non-homologous end joining(NHEJ)of the double-strand breaks of DNA,causing insertional *** random insertional mutant library constructed using this method has become a method of forward genetics for gene ***,the establishment of a random insertional mutant library requires a high transformation efficiency of exogenous *** microalgal species show a low transformation efficiency,making constructing random insertional mutant libraries *** this study,we established a highly efficient transformation method for constructing a random insertional mutant library of Nannochloropsis oceanica,and tentatively tried to isolate its genes to prove the feasibility of the method.A gene that may control the growth rate and cell size was *** method will facilitate the genetic studies of ***,which should also be a reference for other microalgal species.

关键词： Nannochloropsis oceanica genetic transformation random insertional mutant library zeocin pretreatment forward genetics

Configuration Synthesis and Grasping Performance Analysis of Multi-Loop Coupling Capture Mechanism for Launch and Recovery of Torpedo-Shaped Autonomous Underwater Vehicle

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China Ocean Engineering 2024年第3期38卷 453-466页

作者： zhang guo-xing XIA Xin-lu guo Jin-wei School of Mechanical Engineering Jiangsu University of Science and TechnologyZhenjiang 212100China

Research of capture mechanisms with strong capture adaptability and stable grasp is important to solve the problem of launch and recovery of torpedo-shaped autonomous underwater vehicles(AUVs).A multi-loop coupling capture mechanism with strong adaptability and high retraction rate has been proposed for the launch and recovery of torpedo-shaped AUVs with different morphological ***,the principle of capturing motion retraction is described based on the appearance characteristics of torpedo-shaped AUVs,and the configuration synthesis of the capture mechanism is carried out using the method of constrained chain ***,the screw theory is employed to analyze the degree of freedom(DoF)of the capture ***,the 3D model of the capture mechanism is established,and the kinematics and dynamics simulations are carried *** with the capture orientation requirements of the capture mechanism,the statics and vibration characteristics analyses are carried ***,considering the capture process and the underwater working environment,the motion characteristics and hydraulics characteristics of the capture mechanism are ***,a principle prototype is developed and the torpedo-shaped AUVs capture experiment is *** work provides technical reserves for the research and development of AUV capture special equipment.

关键词： capture mechanism torpedo-shaped AUVs configuration synthesis launch and recovery grasping performance analysis

Biology of Hippo signaling pathway:Skeletal muscle development and beyond

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Journal of Integrative Agriculture 2024年第6期23卷 1825-1838页

作者： Shuqi Qin Chaocheng Li Haiyan Lu Yulong feng Tao guo Yusong Han Yongsheng zhang Zhonglin Tang Xinjiang Characteristic Livestock Breeding and Reproduction Team College of Animal Science and TechnologyShihezi UniversityShihezi 832003China Lanzhou Veterinary Research Institute Chinese Academy of Agricultural SciencesLanzhou 730046China Kunpeng Institute of Modern Agriculture at Foshan Foshan 528226China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518124China

Global demand for farm animals and their meat products i.e.,pork,chicken and other livestock meat,is steadily *** the ongoing life science research and the rapid development of biotechnology,it is a great opportunity to develop advanced molecular breeding markers to efficiently improve animal meat production *** is an important study subject because of its crucial role in the regulation of organ *** recent years,with the increase of research on Hippo signaling pathway,the integrative application of multi-omics technologies such as genomics,transcriptomics,proteomics,and metabolomics can help promote the in-depth involvement of Hippo signaling pathway in skeletal muscle development *** Hippo signaling pathway plays a key role in many biological events,including cell division,cell migration,cell proliferation,cell differentiation,cell apoptosis,as well as cell adhesion,cell polarity,homeostasis,maintenance of the face of mechanical overload,*** influence on the development of skeletal muscle has important research value for enhancing the efficiency of animal husbandry *** this study,we traced the origin of the Hippo pathway,comprehensively sorted out all the functional factors found in the pathway,deeply analyzed the molecular mechanism of its function,and classified it from a novel perspective based on its main functional domain and mode of *** aim is to systematically explore its regulatory role throughout skeletal muscle *** specifically focus on the Hippo signaling pathway in embryonic stem cell development,muscle satellite cell fate determination,myogenesis,skeletal muscle meat production and organ size regulation,muscle hypertrophy and atrophy,muscle fiber formation and its transformation between different types,and *** roles in proliferation and regeneration are methodically summarized and analyzed *** summary and prospect of the Hippo signaling pathway within this a

关键词： Hippo skeletal muscle organ size myogenesis C2C12 livestock animals

A new neural-network-based method for structural damage identification in single-layer reticulated shells

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Frontiers of Structural and Civil Engineering 2024年第1期18卷 104-121页

作者： Jindong zhang Xiaonong guo Shaohan ZONG Yujian zhang College of Civil Engineering Tongji UniversityShanghai 200092China China Construction Eighth Engineering Division Co. Ltd.Shanghai 200135China

Single-layer reticulated shells(SLRSs)find widespread application in the roofs of crucial public structures,such as gymnasiums and exhibition *** this paper,a new neural-network-based method for structural damage identification in SLRSs is ***,a damage vector index,NDL,that is related only to the damage localization,is proposed for SLRSs,and a damage data set is constructed from NDL *** the basis of visualization of the NDL damage data set,the structural damaged region locations are identified using convolutional neural networks(CNNs).By cross-dividing the damaged region locations and using parallel CNNs for each regional location,the damaged region locations can be quickly and efficiently identified and the undamaged region locations can be ***,a damage vector index,DS,that is related to the damage location and damage degree,is proposed for *** on the damaged region identified previously,a fully connected neural network(FCNN)is constructed to identify the location and damage degree of *** effectiveness and reliability of the proposed method are verified by considering a numerical case of a spherical *** calculation results showed that the proposed method can quickly eliminate candidate locations of potential damaged region locations and precisely determine the location and damage degree of members.

关键词： single-layer reticulated shell damage identification neural network convolutional neural network cross-partitioning method

Labial inverse dilaceration of bilateral maxillary central incisors: A case report

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World Journal of Clinical Cases 2024年第1期12卷 180-187页

作者： Jun-Min Wang Li-feng guo Li-Qiong Ma Jing zhang Stomatological Hospital and College Key Laboratory of Oral Diseases Research of Anhui ProvinceAnhui Medical UniversityHefei 230032Anhui ProvinceChina Stomatological Hospital and College Anhui Medical UniversityHefei 230032Anhui ProvinceChina

BACKGROUND Dilaceration is a rare dental developmental anomaly characterized by an abrupt deviation along the longitudinal axis of the root in which an angulation forms between the root and the ***,we report on dilacerated bilateral maxi-llary central incisors in mixed *** SUMMARY A 10-year-old girl presented with a chief complaint of unerupted central *** oral examination and radiography provided the basis for a diagnosis of dilaceration of the maxillary central *** surgical exposure of the impacted teeth,a button with an attached chain was applied to the palatal surface of teeth 11 and *** 8 mo,a button was bonded to the labial surface of the crown to fix an elastic chain and move the teeth toward the maxillary ***,a fixed appliance was applied to tooth alignment to Class 1 malocclusion using a 0.019×0.025-inch nickel-titanium *** 3 years of follow-up,the clinical findings and radiographic assessment showed that the roots had developed with vital dental pulp and healthy periodontium,were acceptable aesthetically,and showed no *** The rare occurrences of dilacerated bilateral maxillary central incisors can be successfully treated through surgical exposure and orthodontics.

关键词： Dilaceration Traumatic injury Central incisors Child Primary tooth Case report

Delay-optimal multi-satellite collaborative computation offloading supported by OISL in LEO satellite network

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Journal of Systems Engineering and Electronics 2024年第4期35卷 805-814页

作者： zhang Tingting guo Zijian LI Bin feng Yuan FU Qi HU Mingyu QU Yunbo School of Integrated Circuits and Electronics Beijing Institute of TechnologyBeijing 100081China Yangtze Delta Region Academy Beijing Institute of TechnologyJiaxing 314000China School of Information and Electronics Beijing Institute of TechnologyBeijing 100081China China Research and Development Academy of Machinery Equipment Beijing 100089China

By deploying the ubiquitous and reliable coverage of low Earth orbit(LEO)satellite networks using optical inter satel-lite link(OISL),computation offloading services can be provided for any users without proximal servers,while the resource limita-tion of both computation and storage on satellites is the impor-tant factor affecting the maximum task completion *** this paper,we study a delay-optimal multi-satellite collaborative computation offloading scheme that allows satellites to actively migrate tasks among themselves by employing the high-speed OISLs,such that tasks with long queuing delay will be served as quickly as possible by utilizing idle computation resources in the *** satisfy the delay requirement of delay-sensi-tive task,we first propose a deadline-aware task scheduling scheme in which a priority model is constructed to sort the order of tasks being served based on its deadline,and then a delay-optimal collaborative offloading scheme is derived such that the tasks which cannot be completed locally can be migrated to other idle *** results demonstrate the effective-ness of our multi-satellite collaborative computation offloading strategy in reducing task complement time and improving resource utilization of the LEO satellite network.

关键词： low Earth orbit(LEO)satellite network computation offloading task migration resource allocation

Radiation-based immunogenic vaccine combined with a macrophage“checkpoint inhibitor”for boosting innate and adaptive immunity against metastatic colon cancers

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Acta Pharmaceutica Sinica B 2024年第5期14卷 2247-2262页

作者： Hongbo Xu Xianya Qin Yuanyuan guo Siyu Zhao Xingxing feng Runzan zhang Tianyi Tian Li Kong Conglian Yang Zhiping zhang Tongji School of Pharmacy Huazhong University of Science and TechnologyWuhan 430030China Hubei Engineering Research Center for Novel Drug Delivery System Wuhan 430030China Wuhan Liyuan Hospital of Tongji School of Pharmacy Huazhong University of Science and TechnologyWuhan 430077China

Immunogenic dying tumor cells hold promising prospects as cancer vaccines to activate systemic immunity against both primary and metastatic ***,X-ray-induced dying tumor cells are rich in highly immunogenic tumor-associated antigens and self-generated dsDNA as potent ***,we found that the X-ray induction process can result in the excessive exposure of phosphatidylserine in cancer vaccines,which can specifically bind with the MerTK receptor on macrophages,acting as a“checkpoint”to facilitate immune silence in the tumor ***,we developed a novel strategy combining X-ray-induced cancer vaccines with UNC2250,a macrophage MerTK“checkpoint inhibitor,”for treating peritoneal carcinomatosis in colon *** incorporating UNC2250 into the treatment regimen,immunosuppressive efferocytosis of macrophages,which relies on MerTK-directed recognition of phosphatidylserine on vaccines,was effectively ***,the immune analysis revealed that this combination strategy promoted the maturation of dendritic cells and M1-like repolarization of macrophages,thereby simultaneously eliciting robust adaptive and innate *** innovative approach utilizing X-ray-induced vaccines combined with a checkpoint inhibitor may provide valuable insights for developing effective cancer vaccines and immunotherapies targeting colon cancer.

关键词： Immunogenic cell death Cancer vaccine X-ray Efferocytosis Macrophage STING Adaptive immunity Innate immunity

Cracking on a nickel-based superalloy fabricated by direct energy deposition

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China Foundry 2024年第4期21卷 311-318页

作者： Xue zhang Ya-hang Mu Liang Ma Jing-jing Liang Yi-zhou Zhou Xiao-feng Sun Jin-guo Li School of Materials Science and Engineering University of Science and Technology of ChinaShenyang 110016China Shi-changxu Innovation Center for Advanced Materials Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China School of Materials Science and Engineering Northeastern UniversityShenyang 110819China

Cracks have consistently been a significant challenge limiting the development of additive manufactured nickel-based *** is essential to investigate the location of cracks and their forming *** study extensively examines the impact of solidification process,microstructural evolution,and stress concentration on crack initiation during direct energy deposition(DED).The results emphasize that the crack formation is significantly related to large-angle grain boundaries,rapid cooling *** caused by large-angle grain boundaries and a fast-cooling rate predominantly appear near the edge of the deposited *** cracks are more likely to form near the top of the deposited sample,due to the presence ofγ/γ'*** secondary dendritic arm and the carbides in the interdendritic regions can obstruct liquid flow during the final stage of solidification,which results in the formation of solidification cracks and *** work paves the way to avoid cracks in nickel-based superalloys fabricated by DED,thereby enhancing the performance of superalloys.

关键词： location cracks direct energy deposition nickel-based superalloys

Thoracic spine infection caused by Pseudomonas fluorescens:A case report and review of literature

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World Journal of Clinical Cases 2024年第12期12卷 2099-2108页

作者： Liang Li Bao-Hua zhang Jin-feng Cao Li-Jin zhang Ling-Ling guo Department of Traditional Chinese Medicine Zibo Central HospitalZibo 255000Shandong ProvinceChina Department of Pathology Zibo Central HospitalZibo 255000Shandong ProvinceChina Department of Medical Imaging Zibo Central HospitalZibo 255000Shandong ProvinceChina

BACKGROUND The clinical incidence of spinal infection is gradually increasing,and its onset is insidious,easily leading to missed diagnosis and misdiagnosis,which may lead to serious complications such as nervous system dysfunction,spinal instability and/or deformity,and cause a huge burden on society and *** identification of the causative agent and precision medicine will greatly reduce the suffering of *** present,the main pathogenic bacteria that cause spinal infection are Staphylococcus aureus,Streptococcus,Pneumococcus,Escherichia coli,and *** are no reports of spinal infection caused by Pseudomonas *** SUMMARY We report a 32-year-old female patient with spinal *** presented with flank pain,initially thought to be bone metastases or bone tuberculosis,and had a family background of *** clinical features and changes in imaging and laboratory tests led to the suspicion of thoracic spine *** of the lesion showed inflammation,tissue culture of the lesion was negative several times,and the possible pathogen-Pseudomonas fluorescens was found after gene sequencing of the *** patient recovered completely after a full course of antibiotic *** This report increases the range of pathogens involved in spinal infections,highlights the unique advantages of gene sequencing technology in difficult-todiagnose diseases,and validates conservative treatment with a full course of antibiotics for spinal infections without complications.

关键词： Thoracic spine infection Pseudomonas fluorescens Spinal infection Case report