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斑点叉尾鱼回鱼皮胶原的研究

吉林农业大学学报 2006年第3期28卷 342-345,350页

作者：刘海英李丁过世东江南大学食品学院教育部食品科学与安全重点实验室无锡214036

从斑点叉尾鱼回鱼皮中提取酸溶和酶溶胶原并对其进行了部分定性研究。实验中获得的胶原中甘氨酸最为丰富,质量分数超过23%,酸溶胶原的变性温度为32.5℃,比猪皮胶原的变性温度低5℃。SDS-PAGE实验结果表明酸溶胶原至少有2条不同的α链。... 详细信息

从斑点叉尾鱼回鱼皮中提取酸溶和酶溶胶原并对其进行了部分定性研究。实验中获得的胶原中甘氨酸最为丰富,质量分数超过23%,酸溶胶原的变性温度为32.5℃,比猪皮胶原的变性温度低5℃。SDS-PAGE实验结果表明酸溶胶原至少有2条不同的α链。酸溶胶原和酶溶胶原分别占原料干基的25.8%和38.4%,可以作为猪皮、牛皮胶原的替代品。

关键词：斑点叉尾鱼回胶原氨基酸变性温度

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克氏原螯虾虾头酶解工艺的研究

食品工业科技 2009年第6期30卷 271-274页

作者：钱飞陈焱刘海英过世东江南大学食品学院江苏无锡214122

对克氏原螯虾虾头制取调味料的主要工艺参数进行了研究,同时优化了酶解液脱腥臭配方。确定出最佳水解酶为碱性蛋白酶,最佳酶解的条件为:水解时间1.5h、水解温度65℃、酶与底物比1750U/g、起始pH10.0、固液比1:1,在此条件下,水解率可达41... 详细信息

对克氏原螯虾虾头制取调味料的主要工艺参数进行了研究,同时优化了酶解液脱腥臭配方。确定出最佳水解酶为碱性蛋白酶,最佳酶解的条件为:水解时间1.5h、水解温度65℃、酶与底物比1750U/g、起始pH10.0、固液比1:1,在此条件下,水解率可达41.26%;酶解液脱腥臭最佳配方为:去腥剂为酵母粉,去腥时间为1h,去腥剂质量分数为0.5%,去腥温度为40℃,去腥效果最佳。水解液具有浓郁的虾风味,为调味料较好的基础原料。

关键词：克氏原螯虾虾头酶解去腥臭

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斜张桥动力分析有限元法

同济大学学报 1979年第6期 1-17页

作者：李国豪石洞

本文提出了斜张桥空间挠曲——扭转振动的动力分析方法,刚架式的塔及薄壁箱形梁均离散为梁单元,索单元连系着梁及塔的节点。作者导出了它们的单元刚度矩阵及质量矩阵,并按此法计算了一座斜拉桥的自振频率及振型作为求临界风速的依据,最... 详细信息

本文提出了斜张桥空间挠曲——扭转振动的动力分析方法,刚架式的塔及薄壁箱形梁均离散为梁单元,索单元连系着梁及塔的节点。作者导出了它们的单元刚度矩阵及质量矩阵,并按此法计算了一座斜拉桥的自振频率及振型作为求临界风速的依据,最后对计算结果进行了讨论。

关键词：斜张桥座标扭转振动方程组固有振动斜拉桥结构振动联立方程单元刚度矩阵转换矩阵挠曲单元质量矩阵加劲梁截面积动力分析

Joint Feature Encoding and Task Alignment Mechanism for Emotion-Cause Pair Extraction

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Computers, Materials & Continua 2025年第1期82卷 1069-1086页

作者： shi Li Didi Sun College of Computer and Control Engineering Northeast Forestry UniversityHarbin150040China

With the rapid expansion of social media,analyzing emotions and their causes in texts has gained significant ***-cause pair extraction enables the identification of causal relationships between emotions and their triggers within a text,facilitating a deeper understanding of expressed sentiments and their underlying *** comprehension is crucial for making informed strategic decisions in various business and societal ***,recent research approaches employing multi-task learning frameworks for modeling often face challenges such as the inability to simultaneouslymodel extracted features and their interactions,or inconsistencies in label prediction between emotion-cause pair extraction and independent assistant tasks like emotion and cause *** address these issues,this study proposes an emotion-cause pair extraction methodology that incorporates joint feature encoding and task alignment *** model consists of two primary components:First,joint feature encoding simultaneously generates features for emotion-cause pairs and clauses,enhancing feature interactions between emotion clauses,cause clauses,and emotion-cause ***,the task alignment technique is applied to reduce the labeling distance between emotion-cause pair extraction and the two assistant tasks,capturing deep semantic information interactions among *** proposed method is evaluated on a Chinese benchmark corpus using 10-fold cross-validation,assessing key performance metrics such as precision,recall,and F1 *** results demonstrate that the model achieves an F1 score of 76.05%,surpassing the state-of-the-art by 1.03%.The proposed model exhibits significant improvements in emotion-cause pair extraction(ECPE)and cause extraction(CE)compared to existing methods,validating its *** research introduces a novel approach based on joint feature encoding and task alignment mechanisms,contributing to advancements in emotion-c

关键词： Emotion-cause pair extraction interactive information enhancement joint feature encoding label consistency task alignment mechanisms

Detection and Recognition of Spray Code Numbers on Can Surfaces Based on OCR

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Computers, Materials & Continua 2025年第1期82卷 1109-1128页

作者： Hailong Wang Junchao shi School of Computer Science Zhongyuan University of TechnologyZhengzhou450007China

A two-stage algorithm based on deep learning for the detection and recognition of can bottom spray codes and numbers is proposed to address the problems of small character areas and fast production line speeds in can bottom spray code number *** the coding number detection stage,Differentiable Binarization Network is used as the backbone network,combined with the Attention and Dilation Convolutions Path Aggregation Network feature fusion structure to enhance the model detection *** terms of text recognition,using the Scene Visual Text Recognition coding number recognition network for end-to-end training can alleviate the problem of coding recognition errors caused by image color distortion due to variations in lighting and background *** addition,model pruning and quantization are used to reduce the number ofmodel parameters to meet deployment requirements in resource-constrained environments.A comparative experiment was conducted using the dataset of tank bottom spray code numbers collected on-site,and a transfer experiment was conducted using the dataset of packaging box production *** experimental results show that the algorithm proposed in this study can effectively locate the coding of cans at different positions on the roller conveyor,and can accurately identify the coding numbers at high production line *** Hmean value of the coding number detection is 97.32%,and the accuracy of the coding number recognition is 98.21%.This verifies that the algorithm proposed in this paper has high accuracy in coding number detection and recognition.

关键词： Can coding recognition differentiable binarization network scene visual text recognition model pruning and quantification transport model

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拱——桁梁组合体系空间计算有限元法

同济大学学报 1978年第4期 1-20页

作者：李国豪石洞

针对拱桁梁组合体系桥梁,本文提出一种空间计算方法。方法的物理模型采用连续体,并用有限元求解。整个结构划分为若干组合单元,每个组合单元包括拱、桁梁及竖杆横撑架三部分。本文将演引组合单元刚度矩阵、质量矩阵及内力计算公式。最... 详细信息

针对拱桁梁组合体系桥梁,本文提出一种空间计算方法。方法的物理模型采用连续体,并用有限元求解。整个结构划分为若干组合单元,每个组合单元包括拱、桁梁及竖杆横撑架三部分。本文将演引组合单元刚度矩阵、质量矩阵及内力计算公式。最后与试验结果作了比较,并用精确的空间杆系有限元计算作了验证。

关键词：单元刚度矩阵抗剪刚度撑架转换矩阵质量矩阵平面杆系有限元法空间计算荷载矩阵组合单元局部坐标系大地坐标系组合体系桁梁

Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li dongqing Sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin dong Bin Duan Feiyang Xing Jun Wu Minmin Xiao Fangqing Zhao Jing-dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

鸡源沙门菌的分离鉴定、血清分型和耐药性分析

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中国兽医杂志 2022年第6期58卷 52-56,61页

作者：姬书会郭世栋苏玉贤平顶山市农业综合行政执法支队河南平顶山467000 新乡市动物疫病预防控制中心河南新乡453000 平顶山市畜牧技术推广站河南平顶山467000

为了解河南省部分地区鸡源沙门菌的流行菌株和耐药情况,本试验从平顶山、新乡35个鸡场采集疑似鸡源沙门菌病料154份,进行分离培养、PCR鉴定、血清学分型和药敏试验。结果显示,分离鉴定出48株沙门菌,检出率为31.17%,包括19株鸡白痢沙门菌... 详细信息

为了解河南省部分地区鸡源沙门菌的流行菌株和耐药情况,本试验从平顶山、新乡35个鸡场采集疑似鸡源沙门菌病料154份,进行分离培养、PCR鉴定、血清学分型和药敏试验。结果显示,分离鉴定出48株沙门菌,检出率为31.17%,包括19株鸡白痢沙门菌、13株肠炎沙门菌、7株鼠伤寒沙门菌、3株甲型副伤寒沙门菌和2株乙型副伤寒沙门菌,4株未确定出血清型;耐药性结果显示,分离株对氨苄西林耐药率最高(95.83%);对呋喃唑酮(66.67%)、大观霉素(64.58%)、链霉素(62.50%)也具有较高的耐药性;对氟苯尼考(22.92%)、环丙沙星(12.50%)、复方新诺明(12.50%)、左氧氟沙星(10.42%)耐药率相对较低;对头孢喹肟(8.33%)、头孢曲松(8.33%)、庆大霉素(6.25%)、阿米卡星(6.25%)、多西环素(4.17%)、阿奇霉素(4.17%)和新霉素(2.08%)比较敏感;多重耐药率为77.08%(37/48),最多可耐受8种抗菌药物。本试验结果表明,平顶山和新乡地区鸡源沙门菌的污染率较高,血清型分布广,多重耐药菌株严重,耐药模式多样化,提示应密切关注鸡源沙门菌流行和耐药情况,加强监测,做好综合防控工作。

关键词：沙门菌分离鉴定血清学耐药性

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脂肪替代品在低脂蛋黄酱中的应用研究

食品科学 2009年第10期30卷 82-85页

作者：刘贺朱丹实徐学明过世东金征宇渤海大学生物与食品科学学院辽宁锦州121000 食品科学与技术国家重点实验室江苏无锡214122 江南大学食品学院江苏无锡214122

本实验研究了以明胶和阿拉伯胶为原料通过相分离反应制备的脂肪替代品在低脂蛋黄酱中的应用效果,考察了质构参数和感官性质,结果表明,低脂蛋黄酱的质构参数与全脂对照差别较大,但在感官上没有能被区分出来;通过添加黄原胶0.04%,卡拉胶0.... 详细信息

本实验研究了以明胶和阿拉伯胶为原料通过相分离反应制备的脂肪替代品在低脂蛋黄酱中的应用效果,考察了质构参数和感官性质,结果表明,低脂蛋黄酱的质构参数与全脂对照差别较大,但在感官上没有能被区分出来;通过添加黄原胶0.04%,卡拉胶0.05%做为低脂蛋黄酱的增稠剂,可以解决低脂蛋黄酱的质构缺陷问题。

关键词：脂肪替代品蛋黄酱质构分析感官性质增稠剂