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Mutations of voltage-gated sodium channel contribute to pyrethroid resistance in Panonychus citri

Insect Science 2024年第3期31卷 803-816页

作者： Deng Pan Qiu-Juan Luo Andrias O.O'Reilly guo-rui yuan Jin-Jun Wang Wei Dou Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionAcademy of Agricultural SciencesSouthwest UniversityChongqingChina Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River(Ministry of Education) Academy of Agricultural SciencesSouthwest UniversityChongqingChina School of Biological and Environmental Sciences Liverpool John Moores UniversityLiverpoolUK

Insecticide resistance in Panonychus citri is a major obstacle to mite control in citrus *** insecticides are continually used to control mites in China,although resistance to pyrethroids has evolved in some ***,the resistance to the pyrethroid fenpropathrin was investigated and 7 out of 8 field-collected populations of P citri exhibited a high level of resistance,ranging from 171-fold to 15391-fold higher than the susceptible(SS)comparison *** voltage-gated sodium channel(VGSC)mutations were identified in the tested populations:L1031V,F1747L,and *** sequencing was used to evaluate the frequency of these mutations in the 19 field populations.L1031V and F1751I were present in all populations at frequencies of 11.6%-82.1%and 0.5%-31.8%,respectively,whereas the F1747L mutation was only present in 12 populations from Chongqing,Sichuan,Guangxi,and Yunnan *** of these mutations singly or in combination into transgenic flies significantly increased their resistance to fenpropathrin and these flies also exhibited reduced mortality after exposure to the pyrethroids permethrin andβ-*** citri VGSC homology modeling and ligand docking indicate that F1747 and F1751 form direct binding contacts with pyrethroids,which are lost with mutation,whereas L1031 mutation may diminish pyrethroid effects through an allosteric ***,the results provide molecular markers for monitoring pest resistance to pyrethroids and offer new insights into the basis of pyrethroid actions on sodium channels.

关键词： amplicon sequencing kdr mutation molecular docking Panonychus citri pyrethroid resistance transgenic flies

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Genome-wide analysis of Panonychus citrimicro RNAs witha focus on potential insecticidal activity of 4 micro RNAs toeggs and nymphs

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Insect Science 2024年第2期31卷 354-370页

作者： Chuan-Zhen Li Yu-Hang Liu Deng Pan Meng-Hao Xia Qiang Zhang Yu-Chuang Li guo-rui yuan Jin-Jun Wang Wei Dou Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionAcademy of Agricultural SciencesSouthwestUniversityChongqingChina Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River(Ministry of Education) Academy of Agricultural SciencesSouthwest UniversityChongqingChina

Panonychus citriMcGregor(Acari:Tetranychidae),a destructive citrus pest,causes considerable annual economic losses due to its short lifespan and rapid ***(miRNA)-induced RNA interference is a promising approachfor pest control because of endogenous regulation of pest growth and *** for miRNAs with potential insecticidal activity ***,genome-wide analysis ofmiRNAs at different developmental stages was conducted,resulting in the identificationof 136 miRNAs,including 73 known and 63 novel miRNAs.A total of 17 isomiRNAsand 12 duplicated miRNAs were ***-1 and miR-252-5p were identified asreference miRNAs *** *** on differential expressionanalysis,treatments with miR-let-7a and miR-315 mimics and the miR-let-7a antagomirsignificantly reduced the egg hatch rate and resulted in abnormal egg ***-expression or downregulation of miR-34-5p and miR-305-5p through feeding significantlydecreased the adult eclosion rate and caused molting *** 4 miRNAs,miR-let-7a,miR-315,miR-34-5p,and miR-305-5p,had important regulatory functions and insectici-dal properties in egg hatching and adult *** general,these data advance our un-derstanding of miRNAs in mite biology,which can assist future studies on insect-specificmiRNA-based green pest control technology.

关键词： growth and development microRNAs Panonychus citri referencemiRNAs regulation small RNA libraries

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Reduction behavior of iron ore powder by high-volatile coal in thermogravimetric-gas chromatographic and kinetic analysis

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Journal of Iron and Steel Research(International) 2024年第1期31卷 92-107页

作者： rui-meng Shi Qi-yuan Mi Bin Li Xiao Shi Chong Zou Jing guo Han-jie guo School of Metallurgical Engineering Xi’an University of Architecture and TechnologyXi’an710055ShaanxiChina China International Engineering Consulting Corporation Beijing100048China School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing100083China

The reduction behavior of iron ore powder by high-volatile coal was investigated,and its kinetic mechanism was *** effect of volatiles in coal on the reduction reaction of iron ore was compared by utilizing a Xinjiang lignite with a high volatile content and its pyrolysis carbon produced by high-temperature pyrolysis to remove volatiles,serving as a *** mass loss and gas composition of the samples during the reduction process were detected using thermo-gravimetric analysis and gas chromatography,and the morphological changes of iron ore powder were observed through scanning electron *** kinetic parameters of the iron oxide reduction reaction were calculated by the Flynn-Ozawa-Wall method,and the kinetic mechanism of volatile participation in the iron oxide reduction reaction was determined through the Coats-Redfern *** results indicate that the coupling effect between the high-volatile coal pyrolysis and reduction reactions occurs during the second stage of the entire coupling process,which corresponds to the late stage of coal pyrolysis with a substantial release of H_(2)and *** volatiles in coal actively participated in the reduction reaction,reducing the initiation temperature of the reaction by around 200℃.The reduction of iron oxides by high-volatile coal was jointly promoted by the"hydrogen cycle"and"carbon cycle",resulting in a higher reduction extent and metallization rate at the end of the *** high-volatile coal was used as the reductant,the average activation energy for the entire process was 76.5 kJ/mol,a significant decrease compared to the employment of pyrolysis carbon without volatiles as the reductant(1167 kJ/mol).

关键词： Direct reduction High-volatile coal Volatile Kinetics Reduction mechanism

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Effect of post rolling stress on phase transformation behavior of microalloyed dual phase steel

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Journal of Iron and Steel Research(International) 2024年第3期31卷 688-699页

作者： Wen-quan Sun Sheng-yi Yong Tie-heng yuan Ting-song Yang An-rui He Chao Liu rui-chun guo National Engineering Research Center for Advanced Rolling and Intelligent Manufacturing University of Science and Technology BeijingBeijing 100083China

The slow phase transformation of microalloyed dual phase steel makes the nonuniform stress and temperature fields during the post rolling cooling process have a significant impact on the phase transformation *** the relatively slow phase transformation of DP780 steel within the microalloyed dual phase steel series,the influence of stress on the phase transformation behavior of DP780 steel was *** quantify the nonuniform thermal and stress conditions in the steel coil,a thermo-mechanical coupled finite element model of the hot-rolled strip cooling process was *** on the simulation data,DP780 steel was chosen as the research material,and Gleeble 3500 thermal simulation equipment was used for experimental *** thermal expansion curves were analyzed through regression to establish the dynamic model of DP780 steel phase transformation under ***,metallographic analysis was conducted to determine phase transformation type and grain size of DP780 *** results confirmed that the stress promotes the occurrence of semi-diffusion-type bainite ***,an appropriate level of stress facilitates the growth of bainitic grains,while the increased stress inhibits the growth of ferritic grains.

关键词： Microalloyed dual phase steel Stress Bainitic transformation Grain Finite element method

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Modeling Long- and Short-Term Service Recommendations with a Deep Multi-Interest Network for Edge Computing

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Tsinghua Science and Technology 2024年第1期29卷 86-98页

作者： rui yuan Shunmei Meng ruihan Dou Xinna Wang Department of Computer Science and Engineering Nanjing University of Science and TechnologyNanjing 210056China Faculty of Mathematics University of WaterlooWaterloo N2L 3G1Canada

Edge computing platforms enable application developers and content providers to provide context-aware services(such as service recommendations)using real-time wireless access network *** to recommend the most suitable candidate from these numerous available services is an urgent ***-through rate(CTR)prediction is a core task of traditional service ***,many existing service recommender systems do not exploit user mobility for prediction,particularly in an edge computing *** this paper,we propose a model named long and short-term user preferences modeling with a multi-interest network based on user *** uses a logarithmic network to capture multiple interests in different fields,enriching the representations of user short-term *** terms of long-term preferences,users'comprehensive preferences are extracted in different periods and are fused using a nonlocal *** experiments on three datasets demonstrate that our model relying on user mobility can substantially improve the accuracy of service recommendation in edge computing compared with the state-of-the-art models.

关键词： recommender system logarithmic network nonlocal network

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YOLO-CRD:A Lightweight Model for the Detection of Rice Diseases in Natural Environments

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Phyton-International Journal of Experimental Botany 2024年第6期93卷 1275-1296页

作者： rui Zhang Tonghai Liu Wenzheng Liu Chaungchuang yuan Xiaoyue Seng Tiantian guo Xue Wang Tianjin Key Laboratory of Intelligent Breeding of Major Crops Tianjin Agricultural UniversityTianjin300392China College of Computer and Information Engineering Tianjin Agricultural UniversityTianjin300392China

Rice diseases can adversely affect both the yield and quality of rice crops,leading to the increased use of pesticides and environmental *** detection of rice diseases in natural environments is crucial for both operational efﬁciency and quality *** learning-based disease identiﬁcation technologies have shown promise in automatically discerning disease ***,effectively extracting early disease features in natural environments remains a challenging *** address this issue,this study proposes the YOLO-CRD *** research selected images of common rice diseases,primarily bakanae disease,bacterial brown spot,leaf rice fever,and dry tip nematode disease,from Tianjin *** proposed YOLO-CRD model enhanced the YOLOv5s network architecture with a Convolutional Channel Attention Module,Spatial Pyramid Pooling Cross-Stage Partial Channel module,and Ghost *** former module improves attention across image channels and spatial dimensions,the middle module enhances model generalization,and the latter module reduces model *** validate the feasibility and robustness of this method,the detection model achieved the following metrics on the test set:mean average precision of 90.2%,accuracy of 90.4%,F1-score of 88.0,and GFLOPS of *** the speciﬁc diseases,the mean average precision scores were 85.8%for bakanae disease,93.5%for bacterial brown spot,94%for leaf rice fever,and 87.4%for dry tip nematode *** studies and comparative analyses veriﬁed the effectiveness and superiority of the proposed *** researchﬁnd-ings can be applied to rice disease detection,laying the groundwork for the development of automated rice disease detection equipment.

关键词： Convolutional neural network one stage training rice disease deep learning

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Reliability-based life-cycle cost seismic design optimization of coastal bridge piers with nonuniform corrosion using different materials

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Earthquake Engineering and Engineering Vibration 2024年第1期23卷 209-225页

作者： Wu Xiangtong yuan Wenting guo Anxin Key Lab of Smart Prevention and Mitigation of Civil Engineering Disasters of the Ministry of Industry and Information Technology Harbin Institute of TechnologyHarbin 150090China Key Lab of Structures Dynamic Behavior and Control of the Ministry of Education Harbin Institute of TechnologyHarbin 150090China Institute of Transportation Inner Mongolia UniversityHohhot 010070China

Reinforcement corrosion is the main cause of performance deterioration of reinforced concrete(RC)*** research has been performed to investigate the life-cycle cost(LCC)of coastal bridge piers with nonuniform corrosion using different *** this study,a reliability-based design optimization(RBDO)procedure is improved for the design of coastal bridge piers using six groups of commonly used materials,i.e.,normal performance concrete(NPC)with black steel(BS)rebar,high strength steel(HSS)rebar,epoxy coated(EC)rebar,and stainless steel(SS)rebar(named NPC-BS,NPC-HSS,NPC-EC,and NPC-SS,respectively),NPC with BS with silane soakage on the pier surface(named NPC-Silane),and high-performance concrete(HPC)with BS rebar(named HPC-BS).First,the RBDO procedure is improved for the design optimization of coastal bridge piers,and a bridge is selected to illustrate the ***,reliability analysis of the pier designed with each group of materials is carried out to obtain the time-dependent reliability in terms of the ultimate and serviceability ***,the repair time of the pier is predicted based on the time-dependent reliability ***,the time-dependent LCCs for the pier are obtained for the selection of the optimal design.

关键词： reliability-based design optimization(RBDO) life-cycle cost(LCC) nonuniform corrosion coastal bridge pier repair

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Mechanism and control of nonuniform phase transformation of microalloyed dual-phase steel during cooling process after hot rolling

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Journal of Iron and Steel Research(International) 2024年第2期31卷 428-441页

作者： Wen-quan Sun Sheng-yi Yong Tie-heng yuan Chao Liu San-bao Zhou rui-chun guo Meng-xia Tang National Engineering Research Center for Advanced Rolling and Intelligent Manufacturing University of Science and Technology BeijingBeijing100083China Strip Production Plant Xichang Steel and Vanadium Corporation of Pangang GroupXichang615000SichuanChina

After cooling in the hot rolling process,the metallographic structure of microalloyed dual-phase steel is nonuniform along the rolling direction,while the thickness fluctuation of microalloyed dual-phase steel with a nonuniform metallographic structure will occur during cold *** mechanism of nonuniform phase transformation of microalloyed dual-phase steels was studied during the cooling process after hot rolling,and the nonuniform phase transformation of microalloyed dual-phase steel was regulated during the cooling process after hot rolling through process ***,the empirical equation of phase transformation temperature was measured by a dilatometer considering thermal ***,the phase field and temperature field of laminar cooling process were calculated to provide initial boundary conditions for the finite element *** that,the coupling finite element model of the temperature phase transformation of the strip steel in coiling transportation process was *** simulation results show that the different thermal contact conditions of the microalloyed dual-phase steel during coil transportation lead to uneven cooling of the coil,which leads to nonuniform transformation of the coil along the rolling *** addition,by prolonging the time interval from coiling to unloading,the phenomenon of nonuniform phase transformation of microalloyed dual-phase steel can be effectively *** simulation results are applied to industrial *** application results show that prolonging the time interval from coiling to unloading can effectively improve the nonuniform phase transformation of microalloyed dual-phase steel in the cooling process after hot rolling.

关键词： Microalloyed dual-phase steel Thickness fluctuation Cooling Heat conduction Nonuniform phase transition Finite element method

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Discovery potential of the Glashow resonance in an air shower neutrino telescope

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Chinese Physics C 2024年第8期48卷 266-275页

作者： guo-yuan Huang School of Mathematics and Physics China University of GeosciencesWuhan 430074China Max-Planck-Institut für Kernphysik Saupfercheckweg 169117 HeidelbergGermany

The in-ice or in-water Cherenkov neutrino telescope,such as IceCube,has already proved its power in measuring the Glashow resonance by searching for the bump around E_(ν)=6.3PeV arising from the W-boson *** are many proposals for the next few decades for observations of cosmic tau neutrinos with extensive air showers,also known as tau neutrino *** air shower telescope is,in principle,sensitive to the Glashow resonance via the channel W→τν_(τ)followed by the tau decay in the air(e.g.,TAMBO,which has a geometric area of approximately 500km^(2)).Using a thorough numerical analysis,we find that the discovery significance can be up to 90%with a TAMBO-like setup if PeV neutrinos primarily originate from neutron decays,considering the flux parameters measured by IceCube as the *** presence of new physics affecting the neutrino flavor composition can also increase the ***,if ultrahigh-energy neutrinos are dominantly produced from meson decays,it will be statistically difficult for an advanced proposal such as TAMBO to discriminate the Glashow resonance induced byν¯e from the intrinsicντ/ν¯_(τ)*** have identified several limitations for such advanced telescopes,in comparison with the in-ice or in-water telescope,when measuring resonances:(i)a suppressed branching ratio of 11%for the decay W→τν_(τ);(ii)the smearing effect and reduced acceptance because the daughter neutrino takes away(y)∼75%of the energy from the W decay;and(iii)a large attenuation effect for Earth-skimming neutrinos with the resonance.

关键词： Ultrahigh-energy neutrinos Neutrino telescopes Glashow resonance

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Thermogravimetric characteristics of corn straw and bituminous coal copyrolysis based the ilmenite oxygen carriers

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Chinese Journal of Chemical Engineering 2024年第4期68卷 8-15页

作者： Pengxing yuan Xiude Hu Jingjing Ma Tuo guo Qingjie guo College of Chemical Engineering Qingdao University of Science&TechnologyQingdao 266042China State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering Ningxia UniversityYinchuan 750021China

Herein,the co-pyrolysis reaction characteristics of corn straw(CS)and bituminous coal in the presence of ilmenite oxygen carriers(OCs)are investigated via thermogravimetry coupled with mass *** results reveal that the participation of OCs weakens the devolatilization intensity of *** the CS blending ratio is<50%,the mixed fuel exhibits positive synergistic *** fitting results according to the Coats-Redfern integral method show that the solid-solid interaction between OCs and coke changes the reaction kinetics,enhancing the co-pyrolysis reactivity at the high-temperature zone(750-950C).The synergistic effect is most prominent at a 30%CS blending ratio,with copyrolysis activation energy in the range of 26.35-40.57 kJ·mol^(-1).

关键词： Oxygen carrier Co-pyrolysis Biomass Coal

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