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Neuroscience Bulletin 2015年第6期31卷 763-768页

作者： Jing-Jing Zhou cheng cheng Zilong Qiu Wen-Hao Zhou guo-qiang cheng Department of Neonatology Children's Hospital of Fudan University Institute of Neuroscience Shanghai Institutes for Biological SciencesChinese Academy of Sciences

Neonatal sepsis is common in neonatal intensive care units, often complicated by injury to the immature brain. Previous studies have shown that the expression of the gap junction protein connexin 43 （Cx43） in the brain decreases when stimulated by neuro-inflammatory drugs such as lipopolysaccharide （LPS）. Here we showed that partial deletion of Cx43 in astrocytes resulted in weakened inflammatory responses. The up-regulation of pro-inflammatory cytokines was significantly reduced in mice with partial deletion of Cx43 in astrocytes compared with wild-type littermates after systemic LPS injection. Moreover, microglial activation was inhibited in mice with partial deletion of Cx43. These results showed that Cx43 in astrocytes plays a critical role in neuro-inflammatory responses. This work provides a potential therapeutic target for inhibiting neuro- inflammatory responses in neonatal sepsis.

关键词： astrocyte gap junction Cx43 neonatalsepsis

Apoptosis mechanisms of human gastric cancer cell line MKN-45 infected with human mutant p27

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World Journal of Gastroenterology 2005年第47期11卷 7536-7540页

作者： Jin-Shui Zhu Long Wang guo-qiang cheng Qin Li Zu-Ming Zhu Li Zhu Department of Gastroenterology Affiliated Sixth People's Hospital Shanghai Jiaotong University Shanghai 200233 China

AIM: To explore the inducing effect of human mutant p27 gene on the apoptosis of the human gastric cancer cell line MKN-45 and its associated mechanisms. METHODS: The recombinant adenovirus Ad-p27mt was constructed to infect the human gastric cancer cell line MKN-45. Using flow cytometry, TUNEL assay and DNA fragment analysis, we measured the apoptotic effect of Ad-p27mt on the human gastric cancer cells. RESULTS: Ad-p27mt was successfully constructed and the infection efficiency reached 100%. After 18 h of infection, we observed an apoptotic hypodiploid peak on the flow cytometer before G1-S and apoptotic characteristic bands in the DNA electrophoresis. The apoptotic rate detected by TUNEL method was significantly higher in the Ad-p27mt group (89.4±3.12%)compared to the control group (3.12±0.13%, P ＜ 0.01).CONCLUSION: Human mutant p27 can induce apoptosis of the human gastric cancer cells in vitro.

关键词： Gastric cancer Human mutant p27 Cell line MKN-45

Monkeypox and the perinatal period:what does maternal-fetal medicine need to know?

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World Journal of Pediatrics 2023年第3期19卷 213-223页

作者： Kai Yan Lu-Kun Tang Fei-Fan Xiao Peng Zhang Chun-Mei Lu Li-Yuan Hu Lai-Shuan Wang guo-qiang cheng Wen-Hao Zhou Department of Neonatology Children Hospital of Fudan UniversityNational Children's Medical CenterShanghai 201102China Kunming Medical University Kunming 678400China Department of Neonatology Dehong People's HospitalKunming 650000China Department of Nursing Children's Hospital of Fudan UniversityNational Children's Medical CenterShanghai 201102China Key Laboratory of Neonatal Diseases of National Health Ministry Shanghai 201102China

Background After the global elimination of smallpox,monkeypox has become the most threatening orthopoxvirus to human *** few studies have been reported on pregnant women and *** the case of monkeypox infection,the virus can cause serious adverse pregnancy events in women,which can lead to fetal or neonatal *** sources We made a comprehensive review after an extensive literature search in the PubMed/Medline database and websites concerning smallpox and *** Two case reports reported a total of nine pregnant women,six of whom had fetal *** the autopsy of a stillbirth,researchers found that the placenta was infected with monkeypox virus,but the mechanism of infection remains *** vaccine should be administered to acutely exposed pregnant women and *** novel recombinant vac-cinia immunogloblin(rVIG)and human-specific monoclonal antibodies are being developed for the prevention and treatment of monkeypox virus *** the fetus was delivered,the newborn should take a bath as soon as possible to remove the amniotic fluid and dirt from the *** appropriate isolation protocol for the newborn should be selected according to the infection status of the *** is not known whether monkeypox virus is present in breast milk,and pasteurized breast milk can be given to newborns when breastfeeding is *** This review presents an overview of monkeypox in the perinatal period and guides the future research direction.

关键词： Fetal Maternal Monkeypox Newborn

Effects of SARS-CoV-2 infection on neuroimaging and neurobehavior in neonates

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World Journal of Pediatrics 2021年第2期17卷 171-179页

作者： Kai Yan Fei-Fan Xiao Yu-Wei Jiang Tian-Tian Xiao Da-Jiang Zhang Wen-Hao Yuan Jian-Bo Shao guo-qiang cheng Ling-Kong Zeng Department of Neonatology Children’s Hospital of Fudan UniversityNational Children's Medical CenterShanghaiChina Key Laboratory of Neonatal Diseases of Ministry of Health ShanghaiChina Center for Molecular Medicine Children's Hospital of Fudan UniversityNational Children’s Medical CenterInstitutes of Biomedical SciencesFudan UniversityShanghai.China Institute of Neuroscience Key Laboratory of Primate NeurobiologyCAS Center for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesShanghaiChina Department of Neonatology Chengdu Women's and Children’s Central HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengduChina Department of Radiology Children’s Hospital of Fudan UniversityNational Children's Medical CenterShanghaiChina Department of Neonatology Wuhan Children's Hospital(Wuhan Maternal and Child Healthcare Hospital)Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Department of Radiology Wuhan Children's Hospital(Wuhan Maternal and Child Healthcare Hospital)Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

Background We collected neonatal neurological,clinical,and imaging data to study the neurological manifestations and imaging characteristics of neonates with coronavirus disease 2019(COVID-19).Methods This case-control study included newborns diagnosed with COVID-19 in Wuhan,China from January 2020 to July *** included newborns had complete neurological evaluations and head magnetic resonance *** normalized the extracted T2-weighted imaging data to a standard neonate template space,and segmented them into gray matter,white matter,and cerebrospinal *** comparison of gray matter volume was conducted between the two *** A total of five neonates with COVID-19 were included in this *** median reflex scores were 2 points lower in the infected group than in the control group(P=0.0094),and the median orientation and behavior scores were 2.5 points lower in the infected group than in the control group(P=0.0008).There were also significant differences between the two groups in the total scale score(P=0.0426).The caudate nucleus,parahippocampal gyrus,and thalamus had the strongest correlations with the Hammersmith neonatal neurologic examination(HNNE)score,and the absolute correlation coefficients between the gray matter volumes and each part of the HNNE score were all almost greater than *** We first compared the neurological performance of neonates with and without COVID-19 by quantitative neuroimaging and neurological examination *** the limited numbers of patients,more studies focusing on the structural or functional aspects of the virus in the central nervous system in different age groups will be carried out in the future.

关键词： Coronavirus disease 2019 Magnetic resonance imaging Neonates Quantitative neuroimaging Severe acute respiratory syndrome coronavirus 2

Genetic etiologies associated with infantile hydrocephalus in a Chinese infantile cohort

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World Journal of Pediatrics 2021年第3期17卷 305-316页

作者： Hong-Fang Mei Xin-Ran Dong Hui-Yao Chen Yu-Lan Lu Bing-Bing Wu Hui-Jun Wang guo-qiang cheng Lai-Shuan Wang Yun Cao Lin Yang Wen-Hao Zhou Department of Neonatology Children's Hospital of Fudan UniversityNational Children's Medical CenterShanghaiChina Key Laboratory of Birth Defects Children’s Hospital of Fudan UniversityNational Children’s Medical CenterShanghaiChina Center for Molecular Medicine of Children’s Hospital of Fudan University Institutes of Biomedical SciencesFudan UniversityShanghaiChina Clinical Genetic Center Children’s Hospital of Fudan UniversityNational Children's Medical Center399 Wan Yuan RoadShanghai 201102China

Background Infantile hydrocephalus(IHC)is commonly related to other central nervous system diseases,which may have adverse effects on *** causes of IHC are heterogeneous,and the genetic etiologies are not fully *** study aimed to analyze the genetic etiologies of an IHC *** The data for 110 IHC patients who had received exome sequencing at the Clinical Genetic Center of the Children's Hospital of Fudan University between 2016 and 2019 were reviewed and analyzed *** exome-wide association analysis(EWAS)was performed within this cohort using IHC as the study *** Of the 110 IHC patients,a pathogenic or likely pathogenic variant was identified in 16(15%)patients,spanning 13 *** genes were mainly associated with metabolic disorders,brain abnormalities,and genetic *** patients who had unclear clinical etiology were more likely to possess a genetic *** on previous studies and on our EWAS results,ZEB1,SBF2,and GNAI2 were over-represented among IHC patients and might alfect the signaling pathways involved in IHC *** Our study showed heterogeneous genetic etiologies in an IHC *** is essential to perform genetic testing on IHC patients who have unclear clinical etiology,and genes associated with metabolic disorders,brain abnormalities,and genetic syndromes should he *** addition,when aiming to discover IHC susceptibility genes,genes that might influence the signaling pathways involved in IHC formation should be prioritized.

关键词： Etiology Genetic Hydrocephalus Infantile

Brain development in newborns and infants after ECMO

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World Journal of Pediatrics 2024年第6期20卷 556-568页

作者： Kai Yan Lu-Kun Tang Fei-Fan Xiao Peng Zhang guo-qiang cheng Lai-Shuan Wang Chun-Mei Lu Meng-Meng Ge Li-Yuan Hu Yuan-Feng Zhou Tian-Tian Xiao Yan Xu Zhao-Qing Yin Gang-Feng Yan guo-Ping Lu Qi Li Wen-Hao Zhou Department of Neonatology Children Hospital of Fudan UniversityShanghai 201102China Kunming Medical University Affiliated Dehong Hospital DehongYunnanChina Graduate School Kunming Medical UniversityKunmingYunnanChina Department of Neurology Children's Hospital of Fudan UniversityShanghaiChina School of Medicine Chengdu Women's and Children's Central HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuanChina Department of Intensive Care Medicine Children's Hospital of Fudan UniversityShanghaiChina Department of Intensive Care Medicine The Sixth Medical Center of PLA General HospitalBeijingChina Key Laboratory of Neonatology National Health Care CommissionShanghaiChina

Background Extracorporeal membrane oxygenation(ECMO)not only significantly improves survival rates in severely ill neonates but also is associated with long-term neurodevelopmental *** systematically review the available literature on the neurodevelopmental outcomes of neonates and infants who have undergone ECMO treatment,with a focus on motor deficits,cognitive impairments,sensory impairments,and developmental *** review aims to understand the incidence,prevalence,and risk factors for these problems and to explore current nursing care and management *** sources A comprehensive literature search was performed across PubMed,EMBASE,and Web of Science using a wide array of keywords and phrases pertaining to ECMO,neonates,infants,and various facets of *** initial screening involved reviewing titles and abstracts to exclude irrelevant articles,followed by a full-text assessment of potentially relevant *** quality of each study was evaluated based on its research methodology and statistical ***,citation searches were conducted to identify potentially overlooked *** the focus was primarily on neonatal ECMO,studies involving children and adults were also included due to the limited availability of neonate-specific *** About 50%of neonates post-ECMO treatment exhibit varying degrees of brain injury,particularly in the frontal and temporoparietal white matter regions,often accompanied by neurological *** occur in 18%–23%of neonates within the first 24 hours,and bleeding events occur in 27%–60%of ECMO procedures,with up to 33%potentially experiencing ischemic *** some studies suggest that ECMO may negatively impact hearing and visual development,other studies have found no significant differences;hence,the influence of ECMO remains *** terms of cognitive,language,and intellectual development,ECMO treatment may be associated with potential deve

关键词： Brain development Extracorporeal membrane oxygenation Infants Neonates Neurological outcomes

Risk stratification of hemodynamically significant patent ductus arteriosus by clinical and genetic factors

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World Journal of Pediatrics 2023年第12期19卷 1192-1202页

作者： Yu-Xi Chen Tian-Tian Xiao Hui-Yao Chen Xiang Chen Ya-Qiong Wang Qi Ni Bing-Bing Wu Hui-Jun Wang Yu-Lan Lu Li-Yuan Hu Yun Cao guo-qiang cheng Lai-Shuan Wang Fei-Fan Xiao Lin Yang Xin-Ran Dong Wen-Hao Zhou Center for Molecular Medicine of Children’s Hospital of Fudan University Institutes of Biomedical SciencesFudan University138 Yi Xue Yuan RoadShanghaiChina Department of Neonatology Children’s Hospital of Fudan UniversityNational Children’s Medical CenterShanghaiChina Center for Molecular Medicine Children’s Hospital of Fudan University399 Wanyuan RoadShanghai201102China

Background Hemodynamically significant patent ductus arteriosus(hsPDA)is associated with increased comorbidities in *** evaluation of hsPDA risk is critical to implement individualized *** aim of the study was to provide a powerful reference for the early identification of high-risk hsPDA population and early treatment *** We enrolled infants who were diagnosed with PDA and performed exome *** collapsing analyses were used to find the risk gene set(RGS)of hsPDA for model *** credibility of RGS was proven by RNA *** logistic regression was performed to establish models combining clinical and genetic *** models were evaluated by area under the receiver operating curve(AUC)and decision curve analysis(DCA).Results In this retrospective cohort study of 2199 PDA patients,549(25.0%)infants were diagnosed with *** model[all clinical characteristics selected by least absolute shrinkage and selection operator regression(all CCs)]based on six clinical variables was acquired within three days of life,including gestational age(GA),respiratory distress syndrome(RDS),the lowest platelet count,invasive mechanical ventilation,and positive inotropic and vasoactive *** has an AUC of 0.790[95%confidence interval(CI)=0.749–0.832],while the simplified model(basic clinical characteristic model)including GA and RDS has an AUC of 0.753(95%CI=0.706–0.799).There was a certain consistency between RGS and differentially expressed genes of the ductus arteriosus in *** AUC of the models was improved by RGS,and the improvement was significant(all CCs *** CCs+RGS:0.790 vs.0.817,P<0.001).DCA demonstrated that all models were clinically *** Models based on clinical factors were developed to accurately stratify the risk of hsPDA in the first three days of *** features might further improve the model performance.

关键词： Exome sequencing-Hemodynamically significant patent ductus arteriosus Multivariate logistic regression Neonates Risk stratification

A novel KCNQ2 mutation in familial Early-Onset Epileptic Encephalopathy

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A novel KCNQ2 mutation in familial Early-Onset Epileptic Enc...

Astragalus polysaccharide(APS) supplement in beagle dogs after castration:Effects on the haematology and serum chemistry profiles,immune response,and oxidative stress status

第十三届江浙沪儿科学术会议暨2016年浙江省医学会儿科学学术年会

作者： Yuan-feng Zhou Bing-bing Wu guo-qiang cheng Wen-hao Zhou Yi Wang Department of Neurology Children's Hospital of Fudan University The Molecular Genetic Diagnosis Center Shanghai Key Lab of Birth DefectsPediatrics Research InstituteChildren's Hospital of Fudan University Department of Neonatology Children's Hospital of Fudan University

KCNQ2 gene encoding for voltage-gated potassium channel subunitsKv7.2 and its mutation have been shown to cause benign familial neonatal seizures(BFNS) or epileptic encephalopathy with developmental delay or intellectual disability(ID).Here We report a clinical and Chinese family in which two members were diagnosed with KCNQ2 encephalopathy presenting early onset refractory seizures in the first week of life and neurocognitive *** exome sequencing was used to detect the underlying genetic cause.A novel frame shift mutation c.2422 hetup T was identified in KCNQ2 in two affected *** patients in this family had onset of seizures but the severity of seizures was various and lead to different *** findings suggest that KCNQ2 mutation should be considered in neonates with medicine resistent seizures of unknown cause and contray to previous reports that all mutations published so far are missense mutations,frameshift mutation also can be found in KCNQ2 encephalopathy.

关键词： KCNQ2 Voltage-gated potassium channels Early onset epileptic encephalopathy neurocognitive deficit

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Astragalus polysaccharide(APS) supplement in beagle dogs aft...

Crystal structure determination of β-cyclodextrin-p-aminobenzoic acid inclusion complex from powder X-ray diffraction data

第十六届中国实验动物科学年会

作者： Jianbo Luo Lei Zhang Min Fu Yang Hong Xin-Yin Du guo-qiang cheng Jie-Ying Xia Han Dong Institute of Laboratory Animal Sciences Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Sichuan Academy of Traditional Chinese Medicine Sciences Sichuan Animal Sciences Academy Animal Breeding and Genetics Key Laboratory of Sichuan Province

This study was carried out to determine the effects of supplementing different doses of Astragalus polysaccharide(APS;control,0 mg/kg;APSL,400 mg/kg;and APSH,800 mg/kg) for 8 weeks on the haematology and serum chemistry profiles,immune response,and oxidative stress status in weanling beagle *** on our findings,the APSH diet decreased weight gain(WG) and increased the feed to gain(F:G) ratio in dogs(P <.05).At 14 days after castration,the wound was almost closed,slightly swollen,dry,and clean in the groups supplemented with *** addition,optimal APS supplementation was found to decrease erythrocyte count(RBC),haematocrit(HCT),alkaline phosphatase(ALP),alanine aminotransferase(ALT),C-reactive protein(CRP),interleukin 1β(IL-1β),and tumour necrosis factor-α(TNF-α) levels,and cortisol and protein carbonyl(PC) concentrations(P <.05).Moreover,the mean corpuscular haemoglobin(MCH) and platelet(PLT) levels,interleukin 10(IL-10) and glutathione(GSH) content,and Cu/Zn superoxide dismutase(SOD 1),catalase(CAT),and glutathione peroxidase(Se-GPx) activities were increased in the APS supplemented groups(P<.05).These findings suggest that APS could be used as a feed additive(400 mg/kg diet) for beagle dogs to provide better protection against inflammatory and oxidative stress after weaning.

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Crystal structure determination of β-cyclodextrin-p-aminobe...