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Antitumor effects of human interferon-alpha 2b secreted by recombinant bacillus Calmette-Guérin vaccine on bladder cancer cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第5期13卷 335-341页

作者： guo-qing DING Yan-lan YU Zhou-jun SHEN Xie-lai ZHOU Shan-wen CHEN guo-dong liao Yue ZHANG Department of Urology Sir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhou 310016China Department of Urology Ruijin HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200025China Children's Hospital of Los Angeles Los AngelesCA 90027USA

Objective:Our objective was to construct a recombinant bacillus Calmette-Guérin vaccine(rBCG) that secretes human interferon-alpha 2b(IFNα-2b) and to study its immunogenicity and in vitro antitumor activity against human bladder cancer cell lines T24 and ***:The signal sequence BCG Ag85B and the gene IFNα-2b were amplified from the genome of BCG and human peripheral blood,respectively,by polymerase chain reaction(PCR).The two genes were cloned in Escherichia coli-BCG shuttle-vector pMV261 to obtain a new recombinant plasmid pMV261-Ag85B-IFNα-*** was transformed with the recombinant plasmid by electroporation and designated rBCG-IFNα-*** cells were isolated from human peripheral blood(PBMCs) and stimulated with rBCG-IFNα-2b or wild type BCG for 3 d,and then cultured with human bladder cancer cell lines T24 and *** cytotoxicities were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) ***:BCG was successfully transformed with the recombinant plasmid pMV261-Ag85B-IFNα-2b by electroporation and the recombinant BCG(rBCG-IFNα-2b) was capable of synthesizing and secreting cytokine IFNα-*** proliferation was enhanced significantly by rBCG-IFNα-2b,and the cytotoxicity of PBMCs stimulated by rBCG-IFNα-2b to T24 and T5627 was significantly stronger in comparison to wild type ***:A recombinant BCG,secreting human IFNα-2b(rBCG-IFNα-2b),was constructed successfully and was superior to control wild type BCG in inducing immune responses and enhancing cytotoxicity to human bladder cancer cell lines T24 and *** suggests that rBCG-IFNα-2b could be a promising agent for bladder cancer patients in terms of possible reductions in both clinical dosage and side effects of BCG immunotherapy.

关键词： Bacillus Calmette-Guérin(BCG) vaccine Bladder neoplasms Gene recombination Interferon-alpha 2b

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Preventive effect of gelatinizedly-modified chitosan film on peritoneal adhesion of different types

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World Journal of Gastroenterology 2007年第8期13卷 1262-1267页

作者： Xie-Lai Zhou Shan-Wen Chen guo-dong liao Zhou-Jun Shen Zhi-Liang Zhang Li Sun Yi-Jun Yu Qiao-Ling Hu Xiao-dong Jin Department of Urology The First Affiliated Hospital College of Medicine Zhejiang University Hangzhou 310003 Zhejiang Province China Department of Urology Ruijin Hospital School of Medicine Shanghai Jiaotong University Shanghai 200025 China Surgical Department Clinical Medical College of Hangzhou Teachers College Hangzhou 310036 Zhejiang Province China Experimental Center of Medical Science Hangzhou Teachers College Hangzhou 310036 Zhejiang Province China Institute of Polymer Composites Zhejiang University Hangzhou 310027 Zhejiang Province China

AIM： To comparatively study the preventive effect of gelatinizedly-modified chitosan film on peritoneal adhesions induced by four different factors in rats. METHODS： Chitosan was chemically modified by gelatinization, and made into films of 60 μm in thickness, and sterilized. Two hundred Sprague-Dawley rats were randomly divided into five groups, Sham-operation group （group A）, wound-induced adhesion group （group B）, purified talc-induced adhesion group （group C）, vascular ligation-induced adhesion group （group D）, and infection-induced adhesion group （group E）, respectively. In each group, the rats were treated with different adhesion-inducing methods at the cecum of vermiform processes and then were divided into control and experimental subgroups. Serous membrane surface of vermiform processes were covered with the films in the experimental subgroups, and no films were used in the control subgroups. After 2 and 4 wk of treatments, the abdominal cavities were reopened and the adhesive severity was graded blindly according to Bhatia＇s method. The cecum of vermiform processes were resected for hydroxyproline （OHP） measurement and pathological examination. RESULTS： Adhesion severity and OHP level： After 2 and 4 wk of the treatments, in the experimental subgroups, the adhesions were significantly lighter and the OHP levels were significantly lower than those of the control subgroups in group B （2 wk： 0.199 ± 0.026 vs 0.285 ± 0.041 μg/mg pr, P 〈 0.001; 4 wk： 0.183 ± 0.034 vs 0.276 ± 0.03 μg/mg pr, P 〈 0.001）, D （2 wk： 0.216 ± 0.036 vs 0.274 ± 0.040 μg/mg pr, P = 0.004; 4 wk： 0.211 ± 0.044 vs 0.281 ± 0.047 μg/mg pr, P = 0.003） and E （2 wk： 0.259 ± 0.039 vs 0.371 ± 0.040 μg/mg pr, P 〈 0.001; 4 wk： 0.242 ± 0.045 vs 0.355 ± 0.029 μg/mg pr, P 〈 0.001）, but there were no significant differences in groups A （2wk： 0.141 ± 0.028 vs 0.137 =k 0.026 μg/mg pr, P = 0.737; 4 wk： 0.132 ± 0.031 vs 0.150 ± 0.035 μg/mg pr, P = 0.225） and C （2 wk： 0.395 ± 0.044 vs 0.378 ± 0.043 μg/mg p

关键词： Chitosan Gelatinization Chemical modification Peritoneum Adhesion

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High-temperature-tolerant flexible supercapacitors: Gel polymer electrolytes and electrode materials

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Journal of Energy Chemistry 2025年第1期100卷 426-457页

作者： Chong Peng Xinyi Huang Mingwei Zhao Shuling liao Quanhong Yang Nianjun Yang Siyu Yu School of Chemistry & Chemical Engineering Southwest University Nanoyang Group Tianjin Key Laboratory of Advanced Carbon and Electrochemical Energy Storage School of Chemical Engineering and Technology and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Tianjin University Department of Chemistry & IMO-IMOMEC Hasselt University

The development of flexible supercapacitors(FSCs) capable of operating at high temperatures is crucial for expanding the application areas and operating conditions of supercapacitors. Gel polymer electrolytes and electrode materials stand as two key components that significantly impact the efficacy of hightemperature-tolerant FSCs(HT-FSCs). They should not only exhibit high electrochemical performance and excellent flexibility, but also withstand intense thermal stress. Considerable efforts have been devoted to enhancing their thermal stability while maintaining high electrochemical and mechanical performance. In this review, the fundamentals of HT-FSCs are outlined. A comprehensive overview of state-of-the-art progress and achievements in HT-FSCs, with a focus on thermally stable gel polymer electrolytes and electrode materials is provided. Finally, challenges and future perspectives regarding HT-FSCs are discussed, alongside strategies for elevating operational temperatures and *** review offers both theoretical foundations and practical guidelines for designing and manufacturing HT-FSCs, further promoting their widespread adoption across diverse fields.

关键词： Flexible supercapacitors High-temperature tolerance Gel polymer electrolytes Electrode materials

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Early Paleozoic Granulite-Facies Metamorphism and Magmatism in the Northern Wulan Terrane of the Quanji Massif:Implications for the Evolution of the Proto-Tethys Ocean in Northwestern China

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Journal of Earth Science 2018年第5期29卷 1081-1101页

作者： Qinyan wang Yanjun dong Yuanming Pan Fanxi liao Xiaowei guo School of Earth Sciences Zhejiang UniversityHangzhou 310027China School of Earth Sciences State Key Laboratory of Geological Processes and Mineral ResourcesChina University of GeosciencesWuhan 430074China Hubei Earthquake Administration Wuhan 430071China Department of Geological Sciences University of SaskatchewanSaskatoon S7N 5E2Canada

The nature and evolution of the Proto-Tethys Ocean originated from the breakup of the supercontinent Rodinia remain controversial. Early Paleozoic magmatism and metamorphism can pro- vide important constraints on the closure of the Proto-Tethys Ocean. This paper reports on a set of geological, petrographical, geochronological, mineralogical and geochemical data for Early Paleozoic granite, gabbro, granulite and granitic leucosome in the northern Wulan terrane of the Quanji Massif. Zircon LA-ICP-MS U-Pb dating reveals two episodes of magmatism, with the emplacement of a gran- itic pluton at 476.7±2.8 Ma and a gabbroic dike at 423±2 Ma. Whole-rock geochemistry suggests an arc affinity for the magma of the granitic pluton but a post-collisional extension setting for the gabbroic dike. Zircon LA-ICP-MS U-Pb dating also shows that the peak granulite-facies metamorphism and anatexis occurred at --475 Ma, coeval with the formation of the granitic pluton in the Quanji Massif as well as the early lawsonite-bearing eclogites in the North Qaidam high-pressure and ultrahigh-pressure （HP-UHP） metamorphic belt to the south. The granulite-facies metamorphism with peak P-T condi- tions at 718-729 ℃ and 0.46-0.53 GPa is characterized by an anticlockwise P-T path. Our data provide compelling evidence for Early Paleozoic paired metamorphic belts with HP-UHP metamorphism in the North Qaidam to the south and low PIT metamorphism in the Quanji Massif as a continental arc to the north, hence suggesting a northward subduction polarity for the Proto-Tethys oceanic plate. The intrusion of the post-collisional gabbroic dike supports for the closure of the Proto-Tethys Ocean in north- western China before 423 Ma.

关键词： arc magmatism low P/T granulite-facies metamorphism paired metamorphic belts Proto-Tethys Ocean northern Wulan terrane NW China.

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Impulsive Synchronization of Laser Plasma System

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Communications in Theoretical Physics 2007年第6期47卷 1067-1072页

作者： LI Yang liao Xiao-Feng LI Chuan-dong CHEN guo Department of Computer Science and Engineering Chongqing University Chongqing 400044 China

The issue of impulsive synchronization of the coupled chaotic laser plasma system is investigated. A new framework for impulsive synchronization of such chaotic systems is presented, which makcs the synchronization error system a linear impulsive control system. We derive some suffcient conditions for the synchronization of a laser plasma system via impulsive control with the varying impulsive intervals, which allows us to derive the impulsive synchronization law easily. To illustrate the effectiveness of the proposed results, two numerical examples are given.

关键词： chaos impulsive stabilization impulsive control impulsive synchronization

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Estimation method for random sonic fatigue life of thin-walled structure of a combustor liner based on stress probability distribution

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航空动力学报 2011年第4期26卷 727-734页

作者： SHA Yun-dong guo Xiao-peng liao Lian-fang XIE Li-juan liaoning Key Laboratory of Digital Technology Simulation and Test Technique College of Power and Energy Engineering Shenyang Aerospace University Shenyang 110136 China Shenyang Aeroengine Research Institute Aviation Industry Corporation of China Shenyang 110015 China

As to the sonic fatigue problem of an aero-engine combustor liner structure under the random acoustic loadings,an effective method for predicting the fatigue life of a structure under random loadings was ***,the probability distribution of Von Mises stress of thin-walled structure under random loadings was studied,analysis suggested that probability density function of Von Mises stress process accord approximately with two-parameter Weibull *** formula for calculating Weibull parameters were *** on the Miner linear theory,the method for predicting the random sonic fatigue life based on the stress probability density was developed,and the model for fatigue life prediction was *** an example,an aero-engine combustor liner structure was *** power spectrum density(PSD) of the vibrational stress response was calculated by using the coupled FEM/BEM(finite element method/boundary element method) model,the fatigue life was estimated by using the constructed *** considering the influence of the wide frequency band,the calculated results were *** analysis shows that the estimated results of sonic fatigue of the combustor liner structure by using Weibull distribution of Von Mises stress are more conservative than using Dirlik distribution to some *** results show that the methods presented in this paper are practical for the random fatigue life analysis of the aeronautical thin-walled structures.

关键词： thin-walled structure sonic fatigue life estimation probability density function(PDF) power spectrum density(PSD)

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Transpiration-prompted Photocatalytic Degradation of Dye Pollutant with AuNPs/PANI Based Cryogels

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Chinese Journal of Polymer Science 2022年第10期40卷 1141-1153,I0006,I0005页

作者： Xiao-Xiao guo Shi-Chang Hou Jun Chen Cong liao Wei-dong He CAS Key Laboratory of Soft Matter Chemistry Department of Polymer Science and EngineeringUniversity of Science and Technology of ChinaHefei 230026China

Incautious discharge of organic dyes such as methyl orange(MO)has produced serious pollution to the environment,calling for the efficient techniques to remove them with retaining the green *** photo-catalytic degradation of organic dyes is promising among the developed ***,a strategy based on transpiration-prompted photocatalytic degradation of dye pollutant under sunlight is put ***(ANI)is graft-polymerized onto poly(acrylamide-co-N-4-aminophenylacrylamide)(PAAm)cryogel embedded with gold nanoparticles(AuNPs,diameter:4-10 nm).The obtained cryogels integrated with AuNPs and PANI inside PAAm matrix(AuNP@PAAm-g-PANI)have been structurally explored based on the chemical composition and the phase/porous *** and TEM observation shows that PANI and AuNPs are uniformly distributed in PAAm *** the macro-porosity of cryogel,hydrophilicity of PAAm and photo-thermal activity of PANI,PAAm-g-PANI cryogels without AuNPs can have a photo-thermal evaporation rate of water at 1.63 kg·m^(-2)·h^(-1).As a comparison,Au NP@PAAm-g-PANI cryogels with AuNPs exhibit higher one at 2.20 kg·m^(-2)·h^(-1),suggesting the promotion of AuNPs to photo-thermal ***,PANI appreciatively assists AuNPs to display higher catalytic ability for the oxidative degradation of ***,the removal of MO from water is obviously prompted by the water transpiration under sunlight with AuNP@PAAm-g-PANI cryogels,whose rate constant can reach to 0.320 h^(-1),being three folds of that for the sole absorption of *** transpiration-prompted photocatalytic degradation provides a fascinating route to eliminate organic pollutants and obtain pure water from wastewater simultaneously with sustainable sunlight energy.

关键词： Photothermal evaporation Photocatalytic degradation Sunlight Organic dye Composite cryogel

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Effects of Gap Width and Groove on the Mechanical Properties of Butt Joint Between Aluminum Alloy and Stainless Steel

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Acta Metallurgica Sinica(English Letters) 2013年第2期26卷 143-148页

作者： Honggang dong Chuanqing liao School of Materials Science and Engineering Dalian University of Technology Shanghai Aerospace Equipments manufacturer

Butt joining of 5A02 aluminum alloy to 304 stainless steel sheets was conducted using gas tungsten arc welding process with Al-12%Si （wt.%, the same below） and Zn-15%Al flux-cored filler wires. The effects of gap width and groove in steel side on the microstructure and tensile strength of the resultant joints were investigated. For the joint made with 0 mm-wide gap and without groove in steel side, severe incomplete brazing zone occurred along the steel side and bottom surfaces, and consequently seriously deteriorated the joint strength. However, presetting 1.5 mm-wide gap or with groove in steel side could promote the wetting of molten filler metal on the laying surfaces, and then significantly enhance the resultant joint strength. Moreover, post-weld heat treatment could further improve the tensile strength of the joints. During tensile testing, the specimens from the joints made with AI-12%Si flux-cored filler wire fractured through the weld or interracial layer, but those from the heat-treated joints made with Zn-15%AI flux-cored filler wire fractured in the aluminum base metal.

关键词： Aluminum Stainless steel Arc-brazing Gap width Groove Mechanical property

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In-situ formation of perylene in lacustrine sediments and its geochemical significance

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Acta Geochimica 2020年第4期39卷 587-594页

作者： Jianyang guo Haiqing liao State Key Laboratory of Environmental Geochemistry Institute of GeochemistryChinese Academy of SciencesGuiyang 550002China State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental SciencesBeijing 100012China

To identify the possible sources and formation mechanism of perylene in sediments,temporal trends of perylene and other polycyclic aromatic hydrocarbons,as well as the organic matters,were analyzed in sediment cores from Dianchi Lake and Sihailongwan Maar Lake in *** high concentrations of perylene were observed in both cores and increased gradually with the sediment *** identification suggested that perylene in both cores were of primarily diagenetic origin,other than the anthropogenic *** terrigenous and aquatic organic matters could be the source of perylene in sediments and diagenetic formation of perylene in sediment is kinetically controlled by the microbial activities under anaerobic *** evidence points to the fact that the formation of perylene in sediment should be an index of the microbial activities and environmental conditions,other than the source of specific organic matters or *** addition,we should be cautious when using any geochemical indicators involving the microbial alternation.

关键词： Sediment core Source identification Formation mechanism Organic matters

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Modified Omega-K algorithm for processing helicopter-borne frequency modulated continuous waveform rotating synthetic aperture radar data

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Journal of Systems Engineering and Electronics 2015年第3期26卷 476-485页

作者： dong Li Guisheng liao Yong liao Lisheng Yang Key Laboratory of Aerocraft Tracking Telemetering & Command and Communication Ministry of EducationChongqing University National Lab of Radar Signal Processing Xidian University

With appropriate geometry configuration, helicopter- borne rotating synthetic aperture radar （ROSAR） can break through the limitations of monostatic synthetic aperture radar （SAR） on forward-looking imaging. With this capability, ROSAR has extensive potential applications, such as self-navigation and self-landing. Moreover, it has many advantages if combined with the frequency modulated continuous wave （FMCW） technology. A novel geometric configuration and an imaging algorithm for helicopter-borne FMCW-ROSAR are proposed. Firstly, by per- forming the equivalent phase center principle, the separated trans- mitting and receiving antenna system is equalized to the case of system configuration with antenna for both transmitting and receiving signals. Based on this, the accurate two-dimensional spectrum is obtained and the Doppler frequency shift effect in- duced by the continuous motion of the platform during the long pulse duration is compensated. Next, the impacts of the velocity approximation error on the imaging algorithm are analyzed in de- tail, and the system parameters selection and resolution analysis are presented. The well-focused SAR image is then obtained by using the improved Omega-K algorithm incorporating the accurate compensation method for the velocity approximation error. FJnally, correctness of the analysis and effectiveness of the proposed al- gorithm are demonstrated through simulation results.

关键词： helicopter-borne rotating synthetic aperture radar(ROSAR) frequency modulated continuous wave (FMCW) improved Omega-K algorithm two-dimensional spectrum.

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