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Lycium barbarum polysaccharides protected human retinal pigment epithelial cells against oxidative stressinduced apoptosis

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International Journal of Ophthalmology(English edition) 2015年第1期8卷 11-16页

作者： Lian Liu Wei Lao Qing-Shan Ji Zhi-Hao Yang guo-cheng yu Jing-Xiang Zhong Department of Ophthalmology the First Affiliated Hospital of Jinan University

AIM: To investigate the protective effect and its mechanism of lycium barbarum polysaccharides(LBP)against oxidative stress-induced apoptosis in human retinal pigment epithelial ***: ARPE-19 cells, a human retinal pigment epithelial cell lines, were exposed to different concentrations of H2O2 for 24h, then cell viability was measured by Cell Counting Kit-8(CCK-8) assay to get the properly concentration of H2O2 which can induce half apoptosis of APRE-19. With different concentrations of LBP pretreatment, the ARPE-19 cells were then exposed to appropriate concentration of H2O2, cell apoptosis was detected by flow cytometric analysis. Expression levels of Bcl-2 and Bax were measured by real time quantitative polymerase chain reaction(RT-PCR) ***: LBP significantly reduced the H2O2-induced ARPE-19 cells’ apoptosis. LBP inhibited the H2O2-induced down-regulation of Bcl-2 and up-regulation of ***: LBP could protect ARPE-19 cells from H2O2-induced apoptosis. The Bcl-2 family had relationship with the protective effects of LBP.

关键词： lycium barbarum polysaccharides retinal pigment epithelial cell apoptosis age-related macular degeneration

Effect of nano-selenium loaded with lycium barbarum polysaccharide on the proliferation of lens epithelial cells after UVB damage in vitro

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International Journal of Ophthalmology(English edition) 2022年第1期15卷 9-14页

作者： Jing-Xiang Zhong Shan-Shan Jin Kang-Sheng Wu guo-cheng yu Lei-Lei Tu Lian Liu Department of Ophthalmology the First Affiliated Hospital of Jinan UniversityGuangzhou 510630Guangdong ProvinceChina

AIM:To investigate the effect of nano-selenium loaded with different concentrations of lycium barbarum polysaccharide(LBP-Se NPs)on the proliferation of human lens epithelial cells(HLECs)from UV ***:LBP-Se NPs were prepared and their particle size was ***(SRA01/04)were irradiated with UVB for different time(0,10,20,30,40,50,60 min)to construct a damaged model,the survival rate of cells was determined by methylthiazol tetrazolium(MTT)*** 4’,6-Diamidine-2’-phenylindole dihydrochloride(DAPI)staining was used to observe the status of cell nucleus and drug entering cytoplasm through cell membrane in SRA01/04 cells after adding LBP-SENPS loaded with coumarin fluorescence agent 24 h under fluorescence ***01/04 normal and UVB-damaged cells were treated with different amounts of LBP-Se NPs at different concentrations,cells proliferation were ***:The particle size of LBP-Se NPs was stable in the range of 150-200 *** survival rate changes with time after UVB irradiation were statistically *** 10 min of UVB exposure as the time was chosen to construct the cell damage *** DAPI staining,LBPSe NPs were observed to enter the cytoplasm through the cell membrane under fluorescence inverted *** of SRA01/04 at different concentrations of LBPSe NPs were *** survival rate was statistically different compared with the control *** higher the loading concentration of LBP in nano-Se drugs was,the higher the cell proliferation rate was(P<0.05).The lower the concentration of LBP-Se NPs,the higher the cell proliferation rate,showing a negative growth trend(P<0.05).The group with the highest average cell proliferation rate was 0.5μmol/L 2.0 mg/m L LBP-Se NPs(128.80%).When the 2.0 mg/m L LBP-Se NPs group was selected for cell photography,the cell density was higher at 0.5μmol/*** the increase of concentration,SRA01/04 cells appeared more cytoplasm dehydration,cell shrinkage and ap

关键词： lycium barbarum polysaccharide nano-selenium human lens epithelial cells ultraviolet irradiation

Protective effects of human umbilical cord mesenchymal stem cells on retinal ganglion cells in mice with acute ocular hypertension

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International Journal of Ophthalmology(English edition) 2021年第2期14卷 194-199页

作者： Rui Liu Qi Shi Hong Yang Xiao-yuan Sha guo-cheng yu Lian Liu Jing-Xiang Zhong Department of Ophthalmology the First Affiliated Hospital of Jinan UniversityGuangzhou 510630Guangdong ProvinceChina Department of Ophthalmology Shenzhen HospitalSouthern Medical UniversityShenzhen 518100Guangdong ProvinceChina

AIM:To observe the protective effect of human umbilical cord mesenchymal stem cells(huc MSCs)on retinal ganglion cel s(RGCs)injury in mice with acute ocular hypertension(AOH).METHODS:Fifty-six adult male C57 BL/6 mice were randomly divided into four groups:normal group,AOH group,huc MSCs group,normal saline(NS)*** eye of mice was induced by 90 mm Hg intraocular pressure for 1 h to establish AOH *** MSCs 1×105/μL,1μL or NS 1μL was injected into the vitreous body the next *** fluorescent dye was used to label the 3 rd generation of huc MSCs,for tracing the cells in the vitreous cavity of *** days after the model established,hematoxylin-eosin(HE)staining was used to observe the thickness of the inner retina layer in four *** and loss rate of RGCs were evaluated by counting Brn-3 a positive cells stained by ***:On the 7 th day after AOH established,labeled huc MSCs were found in the vitreous *** staining showed that the thickness of retinal inner layer in AOH group was significantly lower than that in normal group and huc MSCs group(P0.05).Compared with AOH group,the RGCs in normal group was significantly higher;RGCs number increased in huc MSCs group and the loss rate was lower(P<0.05).Injection of NS had no protective effect on ***:In AOH mouse model,vitreous injection of hucMSCs have shown a protection for RGCs.

关键词： human umbilical cord mesenchymal stem cells glaucoma acute ocular hypertension retinal ganglion cells

The association of lumican polymorphisms and high myopia in a Southern Chinese population

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International Journal of Ophthalmology(English edition) 2017年第10期10卷 1516-1520页

作者： Gui-Fang Wang Qing-Shan Ji Bing Qi guo-cheng yu Lian Liu Jing-Xiang Zhong Department of Ophthalmology Loudi Central Hospital of Hunan Department of Ophthalmology the First Affiliated Hospital of Jinan University Department of Ophthalmology the Affiliated Anhui Provincial Hospital of Anhui Medical University

AIM：To investigate the correlation between lumican（LUM）gene and high myopia in a Southern Chinese ***：The study comprised of 95 high myopia patients with a spherical equivalent≤-6.5 diopters（D）.The control group recruited 95 individuals with a spherical equivalent ranging from-0.5 D to＋0.5 *** sequencing was used to detect the single nucleotide polymorphisms（SNPs）of LUM gene in coding *** distributions were tested for Hardy-Weinberg *** and allelic frequencies were analyzed through Chi-square test or Fisher’s exact ***：We identified 3 SNPs of the LUM gene：LUM c.32（rs577456426）,LUM c.507（rs17853500）and LUM c.849（rs181915277）.Among the three SNPs,the genotype and allele frequencies of rs17853500 showed a significant difference between patients and control subjects（P〈0.05）.However,there were no significant differences in rs181915277and rs577456426 between the two groups（P〉0.05）.CONCLUSION：LUM c.507 polymorphism may be a risk factor for the pathogenesis of high myopia in the Southern Chinese population.

关键词： lumican sclem high myopia single nucleotidepolymorphisms

Construction of adenovirus vectors encoding the lumican gene by gateway recombinant cloning technology

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International Journal of Ophthalmology(English edition) 2016年第9期9卷 1271-1275页

作者： Gui-Fang Wang Bing Qi Lei-Lei Tu Lian Liu guo-cheng yu Jing-Xiang Zhong Department of Ophthalmology the First Affiliated Hospital of Jinan University

AIM： To construct adenovirus vectors of lumican gene by gateway recombinant cloning technology to further understand the role of lumican gene in myopia. METHODS： Gateway recombinant cloning technology was used to construct adenovirus vectors. The wild-type （wt） and mutant （mut） forms of the lumican gene were synthesized and amplified by polymerase chain reaction （PCR）. The lumican cDNA fragments were purified and ligated into the adenovirus shuttle vector pDown- multiple cloning site （MCS）-/internal ribozyme entry site （IRES）/enhanced green fluorescent protein （EGFP）. Then the desired DNA fragments were integrated into the destination vector *** yielding the final expression constructs ***-CMV〉wt-lumican/IRES/ EGFP and ***-cytomegalovirus （CMV）〉mutlumican/IRES/EGFP, ***： The adenovirus plasmids ***-CMV〉 wt-lumican/IRES/EGFP and ***-CMV 〉mutlumican/IRESlEGFP were successfully constructed by gateway recombinant cloning technology. Positive clones identified by PCR and sequencing were selected and packaged into recombinant adenovirus in HEK293 cells. CONCLUSION： We construct adenovirus vectors containing the lumican gene by gateway recombinant cloning technology, which provides a basis for investigating the role of lumicangene in the pathogenesis of high myopia.

关键词： myopia lumican adenovirus vector gateway recombinant cloning technology

Dual Toeplitz Algebra on the Polydisk

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Journal of Mathematical Research and Exposition 2008年第2期28卷 366-370页

作者： cheng guo Zheng yu Tao Department of Mathematics Zhejiang Normal University Zhejiang 321004 China

In this paper,we prove the dual Toeplitz algebra I（C（D^-n））contains the ideal к of compact operators as its semicommutator ideal,and study its algebraic *** also get some results about spectrum of dual Toeplitz o... 详细信息

关键词： Bergman space dual Toeplitz operator semicommutator ideal

Comparison of trabeculectomy and trabeculectomy with amniotic membrane transplantation in the same patient with bilateral glaucoma

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International Journal of Ophthalmology(English edition) 2013年第4期6卷 448-451页

作者： Qing-Shan Ji Bing Qi Lian Liu Wei Lao Zhi-Hao Yang Gui-Fang Wang guo-cheng yu Jing-Xiang Zhong Department of Ophthalmology the First Affiliated Hospital of Jinan University

AIM:To observe effects of trabeculectomy with amniotic membrane transplantation(AMT) in controlling postoperative intraocular pressure(IOP) in patients with medically uncontrolled glaucoma.·METHODS:This study included adult patients with requiring bilateral glaucoma *** patient underwent trabeculectomy(Non-AMT group) in one eye and with AMT(AMT group) in the other eye according to randomized *** was defined as intraocular pressure(IOP) <21mmHg without any anti-glaucoma medications at 24 months *** two groups were compared in terms of IOP,complications and success rate.·RESULTS:Thirty-four eyes of 17 patients were investigated in this *** was no statistically signifcant difference in pre-operative IOP between the two *** mean IOP was lower in AMT group compared with Non-AMT group on follow up months 12,18,and *** complications were more frequent in Non-AMT group(35.3%,6/17) compared with AMT group(5.9%,1/17).The success rate of surgery was88.2%(15/17) in Non-AMT group and 100%(17/17) in AMT group.·CONCLUSION:Trabeculectomy with AMT is an effective procedure to reduce IOP and complications,thereby improving surgical success rates.

关键词： trabeculectomy amniotic membrane transplantation glaucoma

Commuting Dual Toeplitz Operators on the Polydisk

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Chinese Quarterly Journal of Mathematics 2009年第1期24卷 63-68页

作者： cheng guo-zheng yu Tao Department of Mathematics Zhejiang Normal University Jinhua 321004 China

We completely characterize commutativity of S and Sψ on La2(Dn)⊥ for bounded pluriharmonic symbols  and ψ on Dn, and prove that SSψ = Sψ if and only if  is analytic or ψˉ is analytic.

关键词： Bergman space dual Toeplitz operator pluriharmonic

Clinical Biobanks, from the World to China

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Biomedical and Environmental Sciences 2014年第6期27卷 481-483页

作者： JI Xu ZHAO Xiu Mei JIANG Jing Jing YIN Ling guo yu cheng Clinical Specimen Bank Translational Medical Center Chinese PLA General Hospital Administrative Office of Chinese PLA General Hospital

The 2009 annual special issue of Time Magazine on March 23rd summarized the ＇10 Ideas Changing the World Right Now＇, in which ＇Biobank＇ had been listed as the eighth. Biobank or biorepository has been developed since last century. Tissue banks represented the early type of clinical biobanks and mainly concentrated on clinical usage such as transplantation at the beginning. The first tissue bank in the United States started in 1949 is the United States Navy Tissue Bank. In 1976, the foundation of American Association of Tissue Banks （AATB） claimed the official show of biobanks on social stage （***）. From the end of 20th Century to the beginning of 21st Century, diverse biobanks of clinical samples, seed banks, gene banks, germplasm banks of non-human species and other various types have sprung up.

关键词： from the World to China World Clinical Biobanks