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Establishment, Growth kinetics, and Susceptibility to AcMNPV of Heat Tolerant Lepidopteran Cell Lines

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Virologica Sinica 2011年第3期26卷 198-205页

作者： Yan-lei WU Lei Jiang Yoshifumi Hashimoto Robert R. Granados guo-xun Li The Center for Advanced Invertebrate Cell Engineering Collaboration with BTI USA Qingdao AgriculturalUniversity Qingdao 266109 China＇~ Boyce Thompson Institute for Plant Research at Cornell University Ithaca NY 14853 USAJ

Lepidopteran heat-tolerant (ht) cell lines have been obtained with sf-9, sf-21 and several Bombyx cells. They have a distinct karyotype, membrane lipid composition, morphology and growth kinetics from the parental cell lines. In this paper, we report the development of ht cell lines from other insect species and examination of their growth characteristics and virus susceptibility. Adaptation of cell lines sf-9, BTI-TN-5B1-4 (High5) and BTI-TN-MG1 (MG1) to 33℃ and 35℃ was carried out by shifting the culture temperature between 28℃ and higher temperatures by a gradual stepwise increase in temperature. The process of adaption to a higher culture temperature was accomplished over a period of 2 months. The cell lines with the temperature adaption were designated as sf9-ht33, sf9-ht35, High5-ht33, High5-ht35, MG1-ht33, MG1-ht35. These cell lines have been subcultured over 70 passages. Adaption to high temperatures was confirmed by a constant population doubling time with individual cell lines. The population doubling time of heat adapted cell lines were 1-4 h less than these of parental cell lines. Cell shapes did not show obvious change, however, the cell size of sf9-ht cells was enlarged and those of High5 and MG1 ht cells were reduced after heat adaption. When the cell lines were infected with Autographa californica nuclear polyhedrosis virus (AcMNPV) at 28℃, 33℃, 35℃ and 37℃, production of budded virus and occlusion bodies in each cell line was optimum at its own adapted temperature.

关键词： Heat-tolerant cell lines Susceptibility of virus Production of virus Growth curves Insect cell lines

Xinhe Mine water inrush risk assessment based on quantification theoretical models

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Journal of Coal Science & Engineering(China) 2010年第4期16卷 386-388页

作者： LI Hui JING guo-xun CAI Zheng-Iong OU Jian-chun School of Safety Science and Engineering Henan Polytechnic University Jiaozuo 454000 China Coal Chemical Industry Group Co. Ltd. Henan Jiaozuo Subsidiary Company Jiaozuo 454000 China School of Safety Science and Engineering China University of Mining and Technology Xuzhou 261116 China

Taking the Xinhe mine's structure, mine pressure, structural fissure, fault andfault displacement, the distance between fault and water inrush point, thickness of block,water pressure those geological factors which influenced the water inrush as the independentvariable, based on these data of water inrush point and water uninrush point, usingthe method of quantification theory(Ⅰ,Ⅱ), it would quantitatively disposes the qualitativevariable, applied to calculation to evaluate the risk of Xinhe's water inrush.

关键词： water inrush assessment prediction quantification theoretical model

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汶川大地震震害特点与成因分析

地震工程与工程振动 2009年第6期29卷 74-87页

作者：郭迅中国地震局工程力学研究所黑龙江哈尔滨150080

2008年5月12日汶川地震造成巨大人员伤亡和财产损失。本文从工程抗震角度分析了震区房屋倒塌的原因,指出地震时房屋倒塌与否与诸多因素有关。在此基础上,分析了汶川地震震害严重的四个主要因素:震级大,烈度高,断层破裂长;震区人口稠密,... 详细信息

2008年5月12日汶川地震造成巨大人员伤亡和财产损失。本文从工程抗震角度分析了震区房屋倒塌的原因,指出地震时房屋倒塌与否与诸多因素有关。在此基础上,分析了汶川地震震害严重的四个主要因素:震级大,烈度高,断层破裂长;震区人口稠密,房屋、路桥等人工工程多;抗震设防水平低;地震诱发地质灾害严重。最后,将汶川地震震害与1976年唐山地震震害作了对比。所得结论可供理解汶川地震震害、改进结构抗震设计参考。

关键词：汶川地震结构抗震震害倒塌地质灾害

The Distribution,Uses,and Characteristic Components of Gentianaceae Plants in China

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World Journal of Traditional Chinese Medicine 2021年第3期7卷 287-298页

作者： Han Cheng Yue-Bin Ge Jun Li Yan Zhang Xian-Ju Huang guo-xun Chen Department of Pharmacology College of PharmacySouth-Central University for NationalitiesWuhan Department of Gatroenterology Affiliated Puren Hospital of Wuhan University of Science and TechnologyWuhanHubeiChina Department of Nutrition University of Tennessee at KnoxvilleTennesseeUSA

Extracts of Gentianaceae herbs have been widely used as food additives,teas,or medicinal remedies for various human diseases and ***,secoiridoids,and analogs glycosides,mainly include gentiopicroside,sweroside,swertiamarin,and loganic acid,are the characteristic compounds found in the Gentianaceae *** uses of the medicinal herbs containing these secoiridoids have been described in Chinese Materia *** extracts containing iridoids,secoiridoids,and analogs could exert protective effects in multiple human tissues and *** of these medicinal herbs have been studied using modern pharmacological *** results indicate that they have analgesic,liver protective,anti-inflammatory,antiallergic,anti-pathogeny,and anti-bacterial *** review was aimed to summarize the use of Gentianaceae herbs for disease treatments and pharmacokinetic characteristics of the active *** so doing,we hope to demonstrate that Gentianaceae herbs and bioactive compounds in them may have abilities to affect inflammation,hepatic metabolism and cell *** addition,we would like to bring this to the attention of the field about the use and study of compounds derived from Gentianaceae herbs.

关键词： Analogs gentiopicroside iridoids phytomedicines secoiridoids

Non-invasive Prenatal Gene Diagnosis: Progress through Cell-free Fetal DNA and RNA in Maternal Plasma and Urine

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中山大学学报（医学科学版） 2008年第S2期29卷 140-142页

作者： guo xun-yang, guo Yi-bin ( Department of Medical Genetics, Zhongshan School of Medicine, SUN Yat-Sen University, Guangzhou 510080, China )

Non-invasive prenatal gene diagnosis has been developed rapidly in the recent years, and numerous medical researchers are focusing on it. Such techniques could not only achieve prenatal diagnosis accurately, but also prevent tangential illness in fetuses and thus, reduce the incidence of diseases. Moreover, it is non-invasive prenatal gene diagnosis that prevents potential threaten and danger to both mothers and fetuses. Therefore, it is welcomed by clinical gynecologist and obstetrian, researchers of medical genetics, and especially, pregnancies. This review article touches briefly on the advanced development of using cell-free DNA, RNA in maternal plasma and urine for non-invasive prenatal gene diagnosis.

关键词： non-invasive prenatal gene diagnosis cell-free fetal DNA and RNA DNA and RNA detection maternal urine maternal plasma

Influence of litter layer on ecological environment in northeastern leymus chinensis grassland of China

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Journal of Life Sciences 2007年第1期1卷 40-48页

作者： WANG Yong guo Ji-xun Key Laboratory of Vegetation Ecology Ministry of Education Institute of Grassland Sciences Northeast Normal University Changchun 130024 China College of Urban and Environmental Science Northeast Normal University Changchun 130024 China

In this paper, we adopted simulation method to discuss influences of litter layer on plants habitat in grassland. Results indicated that ground surface evaporation, soil moisture, surface temperature, soil pH, soil bulk density and soil porosity were all strongly related to the litter quantity. Potassium （K） and organic materials in the soil covered by litter layer were higher than those in the soil uncovered by litter layer. With 100 ***-z increase of litter, the percentage of organic materials increased by 17.9%, nitrogen （N） increased by 7.6%, phosphor （P） increased by 26.4%, and K increased by 3.8%. With the litter accumulation amounting up to 600 g-m-2, the percentage of organic materials increased by 1.8 times, N increased by 81.5%, P increased by 1.8 times and K increased by 26.4%. According to the expected coefficient method of optimization, a mathematical model was established about the optimal accumulation quantity of litter.

关键词： litter layer Leymus chinensis grassland environmental factor soil nutrient

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地震工程试验联网最新进展

地震工程与工程振动 2006年第4期26卷 37-41页

作者：郭迅中国地震局工程力学研究所黑龙江哈尔滨150080

地震工程试验联网(NEES)是新世纪地震工程学科最重要的事件。由美国国家自然科学基金会牵头,全美15家主要的地震工程研究单位共同实施一项旨在调动全部优势资源进行复杂结构体系的地震响应协同试验研究计划。该计划从1999年开始,将于201... 详细信息

地震工程试验联网(NEES)是新世纪地震工程学科最重要的事件。由美国国家自然科学基金会牵头,全美15家主要的地震工程研究单位共同实施一项旨在调动全部优势资源进行复杂结构体系的地震响应协同试验研究计划。该计划从1999年开始,将于2014年全部完成,目前多数成员单位进入设备安装调试阶段,有些单位利用新装备实现了某些利用传统手段无法完成的试验任务。建议工程力学研究所燕郊的结构实验室直接按照加入NEES的标准进行硬件配置和软件开发,使硬件的数量和技术指标满足NEES要求;使软件与硬件配套,实现与NEES联网,可远程快速通讯,实时监控,实时数值模拟计算,实现显式、隐式和显隐混合算法伪动力试验以及具有位移控制、力控制、位移和力混合控制功能。

关键词：伪动力联网试验数据共享实时监控

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海啸破坏特点与工程防御方法初探

自然灾害学报 2005年第1期14卷 175-177页

作者：郭迅中国地震局工程力学研究所黑龙江哈尔滨150080

印度洋海啸现场调查表明,海啸灾害不同于地震和洪水灾害。海啸通过高水位淹没和浪涌冲击对海边地势低平地区的房屋、道路、桥梁、机场以及给排水、供电、通讯等设施与车辆、船只造成严重破坏。海啸上岸后,由于巨大的冲力,将夹带一些破... 详细信息

印度洋海啸现场调查表明,海啸灾害不同于地震和洪水灾害。海啸通过高水位淹没和浪涌冲击对海边地势低平地区的房屋、道路、桥梁、机场以及给排水、供电、通讯等设施与车辆、船只造成严重破坏。海啸上岸后,由于巨大的冲力,将夹带一些破损建筑物产生的固体漂浮物一同前进,因此破坏力更强。由于淹没、浪涌、冲毁建筑物压埋以及漂浮物冲击等综合作用,造成人员死亡率极高,所过之处,财物殆尽。抗御海啸灾害的工程措施主要在于合理规划(避让、削弱、分流、阻挡 )和科学设计 (潜在海啸灾害等级划分、结构性态决策、海啸荷载确定、抗海啸分析、构造设计)。

关键词：海啸工程防御印度洋

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城乡房屋建筑地震风险调查实用方法

地震工程与工程振动 2021年第4期41卷 23-27页

作者：郭迅防灾科技学院中国地震局建筑物破坏机理与防御重点实验室河北三河065201

地震风险调查是我国现阶段社会发展的迫切需求,然而目前还缺少简便实用的调查方法。历史地震震害是最好的老师,作者多年专注于汶川地震研究。基于震害类比、实验验证和理论分析,总结提炼出工程结构抗震能力“散、脆、偏、单”评估法,深... 详细信息

地震风险调查是我国现阶段社会发展的迫切需求,然而目前还缺少简便实用的调查方法。历史地震震害是最好的老师,作者多年专注于汶川地震研究。基于震害类比、实验验证和理论分析,总结提炼出工程结构抗震能力“散、脆、偏、单”评估法,深入分析了权重最高的“偏”的物理机制,编制了便于实际操作的风险调查表。研究结果可供城乡地震风险调查和结构抗震设计参考。

关键词：地震风险震害经验内力分配抗震实验