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镉逆境胁迫下甜菜谷胱甘肽合成酶(BvGS)基因的克隆

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中国糖料 2017年第6期39卷 23-25页

作者：刘大丽马龙彪郭爱英杨洪鲁振强黑龙江省高等学校甜菜遗传育种重点实验室黑龙江大学农作物研究院哈尔滨150080 中国农业科学院北方糖料作物资源与利用重点实验室/中国农业科学院甜菜研究所哈尔滨150080 黑龙江省高等学校生化与分子生物学重点实验室/黑龙江大学生命科学学院哈尔滨150080

利用RT-PCR技术,克隆了甜菜谷胱甘肽合成酶基因(BvGS;LOC104891052),其CDS全长为1 662 bp,编码553个氨基酸。以Beta vulgaris Actin 1为内参基因,半定量RT-PCR的研究结果表明:与0 mM CdCl_2的对照处理相比,在0.5、1.0、1.5、2.0 mM CdCl_2的逆境胁迫下,甜菜BvGS基因的表达量随着处理浓度的增加而逐渐增大。这说明BvGS基因在其转录表达水平上受到了镉胁迫的诱导,并与镉逆境存在着一定的应答关系。

关键词：甜菜基因克隆谷胱甘肽合成酶镉逆境

中性介质中鲁米诺电化学发光监测氧还原过程研究

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苏州科技学院学报（自然科学版） 2003年第4期20卷 48-53页

作者：郭文英徐杨黄炳强屠一锋苏州大学化学化工学院江苏苏州215006

利用硼酸中性缓冲溶液介质中鲁米诺在正矩形脉冲电压激励下的电化学发光行为,研究了溶解氧和H2O2对鲁米诺电化学发光的增强作用。溶解氧在0～16mg/L范围内线性增强鲁米诺的电化学发光,H2O2在5.4×10-8mol/L～2.2×10-7mol/L浓度范围内... 详细信息

利用硼酸中性缓冲溶液介质中鲁米诺在正矩形脉冲电压激励下的电化学发光行为,研究了溶解氧和H2O2对鲁米诺电化学发光的增强作用。溶解氧在0～16mg/L范围内线性增强鲁米诺的电化学发光,H2O2在5.4×10-8mol/L～2.2×10-7mol/L浓度范围内与电化学发光强度呈线性关系,检测限为7.1×10-9mol/L。据此,讨论了氧还原增强鲁米诺ECL的机理并利用鲁米诺电化学发光监测氧还原过程。

关键词：鲁米诺电化学发光中性介质氧还原过程循环伏安法溶解氧发光强度

Characteristics of Mycobacterium tuberculosis serine protease Rv1043c in enzymology and pathogenicity in mice

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Journal of Integrative Agriculture 2023年第12期22卷 3755-3768页

作者： TANG yang-yang CUI ying-ying JIANG Yan-yan SHAO Ming-zhu ZANG Xin-xin DANG Guang-hui LIU Si-guo State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China

The serine proteases of Mycobacteria tuberculosis(Mtb)are important contributors to the process of bacterial invasion and its *** the present study,we systematically characterized the role of the Rv1043c protein in Mycobacterium infection by purifying the Rv1043c protein in Escherichia coli and constructing a Mycobacterium smegmatis(Msg)strain overexpressing Rv1043c(Msg_Rv1043c).We found that Rv1043c had serine protease activity and localized to the surface of *** determined that the optimal pH and temperature for the Rv1043c serine protease were 9.0 and 45°C,***,the serine protease activity of Rv1043c was enhanced by divalent metal ions of Ca^(2+)and Mg^(2+).Site-directed mutagenesis studies demonstrated that the serine 279 residue in Rv1043c plays a catalytic ***,mouse model studies confirmed that Rv1043c significantly enhanced the survival of Msg in vivo,induced pulmonary injury and lung cell apoptosis,and promoted the release of pro-inflammatory cytokines interleukin-1βand interleukin-6 in *** study presents novel insights into the relationship between mycobacterial serine protease and the pathogenesis of the disease.

关键词： Mycobacterium tuberculosis Mycobacterium smegmatis serine protease Rv1043c pathogenicity

Chinese patient with cerebrotendinous xanthomatosis confirmed by genetic testing: A case report and literature review

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World Journal of Clinical Cases 2020年第21期8卷 5446-5456页

作者： Lan-Xiao Cao Mi yang ying Liu Wen-ying Long guo-Hua Zhao Department of Neurology The Fourth Affiliated HospitalZhejiang University School of MedicineYiwu 322000Zhejiang ProvinceChina Central Laboratory The Fourth Affiliated HospitalZhejiang University School of MedicineYiwu 322000Zhejiang ProvinceChina

BACKGROUND Cerebrotendinous xanthomatosis(CTX)is a treatable autosomal recessive inherited metabolic *** results from a deficiency of sterol 27-hydroxylase(CYP27A1),which is a mitochondrial cytochrome P450 enzyme that catalyzes the hydroxylation of cholesterol and modulates cholesterol *** with CYP27A1 deficiency show symptoms related to excessive accumulation of cholesterol and cholestanol in lipophilic tissues such as the brain,eyes,tendons,and vessels,resulting in juvenile cataracts,tendon xanthoma,chronic diarrhea,cognitive impairment,ataxia,spastic paraplegia,and peripheral *** is underdiagnosed as knowledge of the disorder is mainly based on case *** SUMMARY A Chinese family with CTX consisting of one patient and four heterozygous carriers was *** patient is a 47-year-old male,who mainly had psychiatric signs but without some cardinal features of CTX such as cataracts,cerebellar ataxia,pyramidal signs and chronic *** was a significant increase in the concentration of free fatty acid compared to normal *** ultrasound of the urinary system showed multiple left kidney stones,a right kidney cyst,and a hypoechoic area in the bladder,which could move with body *** and axial magnetic resonance imaging(MRI)of the right ankle joint showed apparent enlargement of the right Achilles tendon and upper medial malleolus flexor tendon,abnormal thickening of the plantar fat,and a small amount of exudation around the fascia in front of the Achilles *** MRI suggested white matter(WM)demyelination and slight cerebral *** diagnosis was confirmed by targeted sequencing,which identified compound heterozygous mutations in exon 2 and intron 7 of the CYP27A1 gene(c.435G>T,c.1263+1G>A).Treatment for 3 wk with a combination of lipid-lowering and antipsychotic therapy improved his psychiatric symptoms and normalized the levels of serum free fatty *** in the bladder disapp

关键词： Cerebrotendinous xanthomatosis CYP27A1 Clinical Genetic Cholestanol Case report Metabolic diseases

Free Completely J^(e)-simple Semigroups

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Acta Mathematica Sinica,English Series 2015年第7期31卷 1086-1096页

作者： Jun ying guo Xiao Jiang guo Juan ying DING Department of Mathematics Jiangxi Normal University

A semigroup is called completely J（e）-simple if it is isomorphic to some Rees matrix semi- group over a left cancellative monoid and each entry of whose sandwich matrix is in the group of units of the left cancellative monoid. It is proved that completely J（e）-simple semigroups form a quasivarity. Moreover, the construction of free completely J（e）simple semigroups is given. It is found that a free completely J（e）-simple semigroup is just a free completely J＊-simple semigroup and also a full subsemigroup of some completely simple semigroups.

关键词： Strongly rpp semigroup free semigroup completely J(e)-simple semigroup quasivariety

Micromorphology and physicochemical properties of hydrophobic blasting dust in iron mines

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International Journal of Minerals,Metallurgy and Materials 2019年第6期26卷 665-672页

作者： Jian-guo Liu Long-zhe Jin Jia-ying Wang Sheng-nan Ou Jing-zhong guo Tian-yang Wang School of Civil and Resource Engineering University of Science and Technology BeijingBeijing 100083China Key Laboratory of High-Efficient Mining and Safety of Metal Mines of the Ministry of Education University of Science and Technology BeijingBeijing 100083China Mine Emergency Technology Research Center University of Science and Technology BeijingBeijing 100083China College of Safety Engineering North China Institute of Science and TechnologyYanjiao 101601China

The micromorphology and physicochemical properties of hydrophobic blasting dust(HBD)from an iron mine were comprehensively analyzed by laser particle size analysis(LPSA),scanning electron microscopy(SEM),X-ray diffraction(XRD),and X-ray photoelectron spectroscopy(XPS).The results show that the HBD particles can be classified into three types based on their particle size(PS):larger particles(PS>10μm),medium particles(1μm≤PS≤10μm),and nanoparticles(PS<1μm).The cumulative volume of respirable dust(PS≤10μm)was 84.45%.In addition,three shapes of HBD were observed by SEM:prism,flake,and *** particular,the small particles were mostly flaky,with a greater possibility of being ***,the body and surface chemical compounds of HBD were determined by XRD and XPS,*** adipate(C6H16N2O4)was the only organic compound in the body of HBD,but its mass fraction was only 13.4%.However,the content of organic C on the surface of HBD was 85.35%.This study demonstrated that the small-particle size and large amount of organic matter on the surface of HBD are the main reasons for its hydrophobicity,which can provide important guidance for controlling respirable dust in iron mines.

关键词： iron mine dust respirable dust hydrophobic blasting dust microstructure physicochemical properties particle size

Altered Disease Development in the eui Mutants and Eui Overexpressors Indicates that Gibberellins Negatively Regulate Rice Basal Disease Resistance

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Molecular Plant 2008年第3期1卷 528-537页

作者： Dong-Lei yang Qun Li Yi-Wen Deng Yong-Gen Lou Mu-yang Wang guo-Xing Zhou ying-ying Zhang Zu-Hua He National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academyof Sciences Shanghai China Institute of Insect Science College of Agriculture and Biotechnology Zhejiang University Hangzhou China

Gibberellins （GAs） form a group of important plant tetracyclic diterpenoid hormones that are involved in many aspects of plant growth and development. Emerging evidence implicates that GAs also play roles in stress responses. However, the role of GAs in biotic stress is largely unknown. Here, we report that knockout or overexpression of the Elongated uppermost internode （Eu~ gene encoding a GA deactivating enzyme compromises or increases, respectively, disease resistance to bacterial blight （Xanthomonas oryzae pv. oyrzae） and rice blast （Magnaporthe oryzae）. Exogenous application of GA3 and the inhibitor of GA synthesis （uniconazol） could increase disease susceptibility and resistance, respectively, to bacterial blight. Similarly, uniconazol restored disease resistance of the eui mutant and GA3 decreased disease resistance of the Eui overexpressors to bacterial blight. Therefore, the change of resistance attributes to GA levels. In consistency with this, the GA metabolism genes OsGA2Oox2 and OsGA20xl were down-regulated during pathogen challenge. We also found that PRla induction was enhanced but the SA level was decreased in the Eui overexpressor, while the JA level was reduced in the eui mutant. Together, our current study indicates that GAs play a negative role in rice basal disease resistance, with EUI as a positive modulator through regulating the level of bioactive GAs.

关键词： SYSTEMIC ACQUIRED-RESISTANCE SALICYLIC-ACID CELL-DEATH SIGNALING PATHWAY GREEN-REVOLUTION DEPENDENT GROWTH

Formation of G-phase in 20Cr32Ni1Nb Stainless Steel and its Effect on Mechanical Properties

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Acta Metallurgica Sinica(English Letters) 2017年第9期30卷 829-839页

作者： Xiao-Feng guo ying-ying Ni Jian-Ming Gong Lu-yang Geng Jian-Qun Tang Yong Jiang Xian-Kai Jia Xin-Yu yang School of Mechanical and Power Engineering Nanjing Tech UniversityNanjing 211816China Key Lab of Design and Manufacture of Extreme Pressure Equipment Nanjing 210037China

A series of tensile tests, Charpy impact tests, optical microscopy observations, and field emission-scanning electron microscopy examinations, were carded out to investigate the mechanical properties and microstructural evolution of 20Cr32NilNb steel. Experimental results indicate that the as-cast microstructure of the steel typically consists of a supersaturated solid solution of austenite matrix with a network of interdendritic primary carbides （NbC and M23C6）. In the ex-service samples, large amounts of secondary carbides precipitate within austenite matrix. Besides the growth and coarsening of NbC and M23C6 carbides during service condition, the Ni-Nb silicides known as G-phase （Nil6Nb6Si7） are formed at the interdendritic boundaries. The microstructural evolution results in the degradation of the mechanical properties of the ex-service steel. In addition, the precipitate rate of G-phase, depending in part on Si content, varies greatly for the 20Cr32NilNb steel, which plays a key role in the long-term microstructural stability of the steel. Based on the X-ray diffraction data, time-temperature-transformation curve for the steel is obtained from the aged specimens.

关键词： 20Cr32NilNb Embrittlement G-phase TTT dial, ram

Vortex identification based on the Liutex method and its effect on fish passage upstream

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Journal of Hydrodynamics 2024年第1期36卷 130-141页

作者： Chun-ying Shen Rui-guo yang Xiao-tao Shi Ming-ming Wang Shi-hua He Faculty of Electrical Power Engineering Kunming University of Science and TechnologyKunming650500China Hubei International Science and Technology Cooperation Base of Fish Passage China Three Gorges UniversityYichang443002China Shanghai Investigation Design and Research Institute Co.LtShanghai200434China

Fishway research is important for mitigating the fragmentation of river habitats caused by hydraulic *** vertical slit fishway is a broadly used fishway type because of its high efficiency and adaptability to water ***,the resulting vortex current disrupts the fish passage hence directly affecting fish *** study aims to accurately capture the vortex structure in the fishway and analyze the effect of vortex elements(vortex structure,vortex intensity,etc.)on *** conducted an analysis of the 3-D current flow field in the fishway through the utilization of an experimental model and the large eddy simulation(LES)***,we captured the vortex information in the fishway at different flow rates using the Liutex vortex identification method and investigated the effect of the vortex on fish *** results revealed that the structures inside the fishway pool occupy most of the room,however,the areas with higher vortex strength were primarily located in the vortex near the vertical seam and the mainstream,the vortex strength inside the fishway gradually increases with increasing flow,suppressing fish *** experienced significantly increased resistance when encountering strong *** suggests that the vortex may act as a physical barrier to fish *** findings highlight the potential negative effects of vortex on fish movement and reiterate the importance of understanding vortex dynamics for aquatic environmental *** an effective tool for identifying vortices in fluid flow,the Liutex method demonstrates features of vortex within the fishway,thereby providing important insights into the interaction between fluid dynamics and aquatic organisms.

关键词： Vertical slit fishway vortex identification method Liutex large eddy simulation(LES) fish experiment computational fluid dynamics