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Determination of Doxycycline in Doxycycline Hydrochloride Soluble Powder under Different Chromatographic Conditions

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Agricultural Biotechnology 2022年第5期11卷 121-129页

作者： Lanying guo Youjiang DIAO shijin guo Shandong Agriculture And Engineering University Jinan 250100China Shandong Binzhou Animal Science&Veterinary Medicine Academy Binzhou 256600China Shandong Lvdu Ante Animal Veterinary Drug Industry Binzhou 256600China

[Objectives]This study was conducted to compare the accuracy and detection speed of high performance liquid chromatography for determining the content of doxycycline hydrochloride in doxycycline hydrochloride soluble powder under different chromatographic conditions, in order to improve the level of laboratory testing. [Methods] Four sets of experiments were designed through a cross-test method. The contents of doxycycline hydrochloride in the raw materials of doxycycline hydrochloride and doxycycline hydrochloride soluble powder were determined by changing the chromatographic column and mobile phase conditions, and the feasibility and practicability of the four methods were judged by comparing the detection results through data and chromatographic signal processing. [Results] The content of doxycycline hydrochloride in finished doxycycline hydrochloride soluble powder and the raw materials of doxycycline hydrochloride could be accurately determined under the four chromatographic conditions, of which the experimental group using Agilent C18 column and the mobile chromatography conditions of finished doxycycline hydrochloride soluble powder had the shortest retention time. [Conclusions] The high performance liquid chromatography method using Agilent C18 chromatographic column and mobile phase chromatographic conditions of finished doxycycline hydrochloride soluble powder products can quickly and accurately determine the content of doxycycline hydrochloride in doxycycline hydrochloride soluble powder.

关键词： Doxycycline hydrochloride Chromatographic conditions High performance liquid chromatography

Bactericidal Effect and Clinical Application of 20% Sodium Dichloroisocyanurate Powder

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Agricultural Biotechnology 2022年第5期11卷 50-53页

作者： Chengxin LYU Youjiang DIAO shijin guo Shandong Agriculture and Engineering University Jinan 250100China Shandong Binzhou Animal Science and Veterinary Medicine Academy Binzhou 256600China Shandong Lvdu Ante Veterinary Drug Industry Binzhou 256600China

[Objectives]This study was conducted to investigate the formulation stability of 20% sodium dichloroisocyanurate powder and its killing effect on common pathogenic bacteria in the animal breeding environment and to provide a scientific basis for veterinary clinical application. [Methods] The stability of 20% sodium dichloroisocyanurate powder was tested through an accelerated test and a long-term test. In the accelerated test, 20% sodium dichloroisocyanurate powder and sodium hypochlorite were stored in 37 ℃ constant temperature incubators for 90 d, and the available chlorine contents were compared. In the long-term test, 20% sodium dichloroisocyanurate powder was sealed and stored at room temperature, and its effective chlorine content and pH were measured regularly. In order to test its bactericidal effect in clinical use, 20% sodium dichloroisocyanurate powder, lime milk and 5% dilute glutaraldehyde solution were sprayed in groups to disinfect empty sheds in a pig farm, and the total colony count, Escherichia coli colony count, Staphylococcus aureus colony count and fungal colony count were used as testing indexes to comprehensively evaluate the disinfection effects on microorganisms in the air of the empty sheds and the surface of objects inside the sheds after disinfection. [Results] The 20% sodium dichloroisocyanurate powder had an available chlorine loss rate of 7.92% in the accelerated test, and the loss rates of available chlorine content and pH of 20% sodium dichloroisocyanurate powder in the long-term test(240 d) were 1.52% and 1.87% at room temperature, respectively. In the test to detect the sterilization effects on the pig farm, the total colony count, E. coli colony count, S. aureus colony count and fungal colony count of samples treated with 20% sodium dichloroisocyanurate powder were lower than those of other two groups(P<0.05), and the total colony count of samples disinfected with 20% sodium dichloroisocyanurate powder after cleaning the dirt was

关键词： Sodium dichloroisocyanurate Stability Disinfection Clinic application

Study on Strengthening Animal Disease Prevention

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Agricultural Biotechnology 2022年第3期11卷 69-71页

作者： Qing LI Daoming LI shijin guo Changyi Animal Husbandry Development Center Weifang 261300China Agricultural and Rural Comprehensive Service Center of Bincheng District Binzhou CityBinzhou 256600China Binzhou Institute of Animal Husbandry and Veterinary Medicine Binzhou 256600China

With the in-depth development of China’s market economy,animal husbandry has become an important industry that affects China’s economic *** order to effectively promote the healthy development of animal husbandry,attention should be paid to the prevention of animal *** paper made a detailed analysis from the main problems encountered in the process of animal epidemic prevention work as an entry point,and put forward some effective measures for strengthening animal epidemic prevention work.

关键词： Animal disease Preventive work Strengthening Preventive measures

NEUMANN PROBLEM FOR THE LANDAU-LIFSHITZ-MAXWELL SYSTEM IN TWO DIMENSIONS

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Chinese Annals of Mathematics,Series B 2001年第4期22卷 529-540页

作者： guo BOLING, DING shijin Center of Nonlinear Studies, Institute of Applied Physics and Computational Mathematics, P. O. Box 8009, Beijing 100088, China. E-mail: gbl@ *** Center of Nonlinear Studies Institute of Applied Physics and Computational Mathematics P. O. Box 8009 Beijing 100088 China

This paper is concerned with the global existence and the partial regularity for the weak solution of the Landau-Lifshitz-Maxell system in two dimensions with Neumann boundary conditions.

关键词： Landau-Lifshitz-Maxwell system Neumann problem Global existence

Preparation and Quality Evaluation of Toltrazuril Nanoemulsion

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Animal Husbandry and Feed Science 2015年第5期7卷 314-316页

作者： Zhang Zhimei Deng Xuming Shen Zhiqiang guo shijin Fu Shijun College of Animal Science and Veterinary Medicine Jilin University Shandong Binzhou Animal Science and Veterinary Medicine Academy Shandong Binzhou Research Development and Promotion Center for Livestock and Poultry Propolis Vaccine Shandong Lvdu Ante Veterinary Drug Co. Ltd.

To determine the prescription and preparation method of toltrazuril nanoemulsion, we selected liquid paraffin, ethyl acetate, soybean oil, IPM and oleic acid as the oil phases, while RH-40, EL-40, OP-10, Tween-80 and Span-80 were the surfactants, and alcohol, 1,2-ropanediol, ispropanl, glycerol and PEG-400 were the co-surfactants. The best prescription was acquired through pseudo-ternary phase diagrams. The result showed that ethyl acetate, Tween-80 and 1,2-propanediol could facilitate the formation of nanoemulsion. The optimum prescription was surfactant：co-surfactant：oil phase：water phase =2：1：2：5, with the solubilizer of 10%. This prescription can prepare stable toltrazuril nanoemulsion.

关键词： Toltrasuril Nanoemulsion Preparation Quality evaluation

Simultaneous Determination of Sulfamonomethoxine Sodium and Trimethoprim in Compound Sulfamonomethoxine Sodium Propolis Solution by High Performance Liquid Chromatography(HPLC)

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Animal Husbandry and Feed Science 2015年第4期7卷 238-240,245页

作者： guo shijin Zhang Zhimei Fu Shijun Tang Shiyun Shen Zhiqiang Shandong Binzhou Animal Science and Veterinary Medicine Academy Shandong Lvdu Ante Veterinary Drug Co. Ltd.

[Objective] The paper aimed to provide a basis for quality control of compound sulfamonomethoxine sodium propolis solution. [Method] High per- formance liquid chromatography （HPLC） method was used to determine the contents of sulfamonomethoxine sodium and trimethoprim simultaneously in compound sulfamonomethoxine sodium propolis solution. [Result] Sulfamonomethoxine sodium and trimethoprim showed a linear relationship within the concentration of 10 - 500 μg/mL under the following chromatographic conditions： Kromasil C18 column （250 mm × 4.6 mm, 5 μm）, mobile phase 0.02 mol/L phosphoric acid solution- methanol（80：20, V/V）, detection wavelength 270 nm, flow rate 1.0 mL/min, and column temperature 30℃. The average recoveries were 99.3% and 99.4%, respectively. [ Conclusion] The method is accurate and feasible, and can determine sulfamonomethoxine sodium and trimethoprim simultaneously in compound sul- famonomethoxine sodium propolis solution.

关键词： Sulfamoanmethoxine sodium Trimethopfim HPLC

Progress in the Application of Nano-trace Elements in Animal Husbandry

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Animal Husbandry and Feed Science 2022年第1期14卷 45-49页

作者： guo shijin Zhang Zhimei Fu Shijun Tang Shiyun Li Ling Shandong Binzhou Animal Science&Veterinary Medicine Academy Binahou 256600China Shandong Lvdu Anie Veterinary Drug Industry Co. Ltd.Binzhou 256600China

Nano-trace element additives, with a particle size of 1-100 nm, are absorbed directly by osmosis in animals without ion exchange. When added to the feed, they can not only promote the growth by own nutrition at the conventional size, but also greatly improve the utilization and ab-sorption rate of additives by animals. In this paper, the researches on micro-element feed additives such as nano-selenium, nano-copper, nano-zinc and nano-chromium were reviewed, which will provide basis for breeding production.

关键词： Nano Trace elements Animal husbandry Application

Disinfection Effects and Clinical Application of Sodium Dichloroisocyanurate Powder on Bacteria

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Agricultural Biotechnology 2022年第4期11卷 57-60页

作者： shijin guo Ling LI Qing LI Jingjing ZHOU Chengxin LYU Lanying guo Zhiqiang SHEN Shandong Binzhou Animal Science and Veterinary Medicine Academy Binzhou 256600China Shandong Lvdu Ante Veterinary Drug Industry Binzhou 256600China Changyi Animal Husbandry Development Center Weifang 261300China Shandong Agriculture and Engineering University Jinan 250100China

With the continuous increase of large-scale pig farms,the disinfection of pig farm environment plays an extremely important part in the control and prevention of pig farm diseases in high-density breeding *** an important breeding place for pig farms,the farrowing house must be performed with scientific and standardized *** this paper,different disinfection modes were used to disinfect the empty farrowing houses of pig farms,and the total plate count,Escherichia coli,Staphylococcus aureus,and fungi were used as detection indicators to comprehensively evaluate the disinfection effect of the air microorganisms and the surface of the objects in the empty house after disinfection,with the aim to provide a basis for the establishment of a scientific pig farm environmental disinfection model.

关键词： Sodium dichloroisocyanurate powder Bacteria Disinfection effect Pig farm Clinical application

Harms of Mycotoxins to Animals and Research Progress of Detection Methods

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Animal Husbandry and Feed Science 2022年第4期14卷 21-24页

作者： guo shijin Zhang Zhimei Tang Shiyun Fu Shijun Li Ling Shen Zhiqiang Shandong Binzhou Animal Science&Veterinary Medicine Academy Binzhou 256600China Shandong Lvdu Ante Veterinary Drug Industry Co. Ltd.Binzhou 256600China

Mycotoxin is widely distributed in various feedstuffs and feeds of *** not only causes feed deterioration,but also leads to various diseases of animals,or even results in acute toxic death,causing great harm to the breeding *** this paper,the harm of mycotoxin to animals and its detection methods in feed and animals are summarized and analyzed,so as to provide the reference basis for further improving the detection methods of mycotoxin and reducing the harm of mycotoxin to breeding industry.

关键词： Mycotoxin Harm Detection method