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Molecular Cloning and Characterization of an Allene Oxide Cyclase Gene Associated with Fiber Strength in Cotton

Journal of Integrative Agriculture 2014年第10期13卷 2113-2121页

作者： wang li-man Zhu You-min TONG Xiang-chao hu Wen-jing CAI Cai-ping guo wang-zhen State Key Laboratory of Crop Genetics & Germplasm Enhancement Hybrid Cotton R&D Engineering Research CenterMinistry of Education/College of AgricultureNanjing Agricultural University

Allene oxide cyclase （AOC） is one of the most important enzymes in the biosynthetic pathway of the plant hormone jasmonic acid （JA）. AOC catalyzes the conversion ofallene oxide into 12-oxo-phytodienoic acid （OPDA）, a precursor of JA. Using 28K cotton genome array hybridization, an expressed sequence tag （EST; GenBank accession no. ES792958） was investigated that exhibited significant expression differences between lintless-fuzzless XinWX and linted-fuzzless XinFLM isogenic lines during fiber initiation stages. The EST was used to search the Gossypium EST database （http：//***/） for corresponding cDNA sequences encoding full-length open reading frames （ORFs）. Identified ORFs were confirmed using transcriptional and genomic data. As a result, a novel gene encoding AOC in cotton （Gossypium hirsutum AOC; GenBank accession no. KF383427） was cloned and characterized. The 741-bp GhAOC gene comprises three exons and two introns and encodes a polypeptide of 246 amino acids. Two homologous copies were identified in the tetraploid cotton species G. hirsutum acc. TM-1 and G. barbadense cv. Hai7124, and one copy in the diploid cotton species G. herbaceum and G. raimondii, qRT-PCR showed that the GhAOC transcript was abundant in cotton fiber tissues from 8 to 23 days post anthesis （DPA）, and the expression profiles were similar in the two cultivated tetraploid cotton species G. hirsutum acc. TM- 1 and G. barbadense cv. Hai7124, with a higher level of transcription in the former. One copy of GhAOC in tetraploid cotton was localized to chromosome 24 （Chr. D8） using the subgenome-specific single nucleotide polymorphism （SNP） marker analysis, which co-localized GhAOC to within 10 cM of a fiber strength quantitative trait locus （QTL） reported previously. GhAOC was highly correlated with fiber quality and strength （P=0.014） in an association analysis, suggesting a possible role in cotton fiber development, especially in secondary cell wall thickening.

关键词： allene oxide cyclase structure expression pattern fiber development association analysis cotton

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A Strigolactone Biosynthesis Gene Contributed to the Green Revolution in Rice

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Molecular Plant 2020年第6期13卷 923-932页

作者： Yuexing wang lianguang Shang Hong Yu Longjun Zeng Jiang hu Shen Ni Yuchun Rao Sanfeng li Jinfang chu Xiangbing Meng Lei wang Ping hu Jijun Yan Shujing Kang Minghao Qu Hai lin Tao wang Quan wang Xingming hu Hongqi Chen Bing wang Zhenyu Gao Longbiao guo Dali Zeng Xudong Zhu guosheng Xiong Jiayang li Qian Qian State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China State Key Laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing)Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Plant Phenomics Research Center Nanjing Agricultural UniversityNanjing 210095China college of Chemistry and life sciences Zhejiang Normal UniversityJinhua 321004China

Plant architecture is a complex agronomic trait and a major factor of crop yield,which is affected by several important ***(SLs)are identified as a new class hormoneinhibiting branching in many plant species and have been shown to be involved in various developmental *** and chemical modulation of the SL pathway is recognized as a promising approach to modify plant ***,whether and how the genes involved in the SL pathway could be utilized in breeding still remain ***,we demonstrate that a partial loss-of-function allele of the SL biosynthesis gene,HIGH TILLERING AND DWARF 1/DWARF17(HTD1/D17),which encodes CAROTENOID CLEAVAGE DIOXYGENASE 7(CCD7),increases tiller number and improves grain yield in *** found that the HTD1 gene had been widely utilized and co-selected with Semidwarf 1(SD1),both contributing to the improvement of plant architecture in modern rice varieties since the Green Revolution in the *** how phytohormone pathway genes regulate plant architecture and how they have been utilized and selected in breeding will lay the foundation for developing the rational approaches toward improving crop yield.

关键词： rice strigolactones tiller number Green Revolution

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Modulation of nitrate-induced phosphate response by the MYB transcription factor Rli1/HINGE1 in the nucleus

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Molecular Plant 2021年第3期14卷 517-529页

作者： Zhihua Zhang Zhao li Wei wang Zhimin Jiang liping guo Xiaohan wang Yangwen Qian Xiahe huang Yongqiang liu Xiujie liu Yahong liu Aifu li Yu Yan Junpeng Xie Shouyun Cao Stanislav Kopriva Legong li Fanjiang Kong Baohui liu Yingchun wang Bin hu Chengcai chu State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biologythe Innovative Academy for Seed DesignChinese Academy of SciencesBeijingChina School of life sciences Guangzhou University.GuangzhouChina college of Plant science Jilin UniversityChangchunChina Biogle Genome Editing Center ChangzhouChina State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental Biologythe Innovative Academy for Seed DesignChinese Academy of SciencesBeijingChina college of Advanced agricultural science University of Chinese Academy of SciencesBeijingChina Botanical Institute Cluster of Excellence on Plant SciencesUniversity of CologneCologneGermany college of life sciences Capital Normal UniversityBeijingChina

The coordinated utilization of nitrogen(N)and phosphorus(P)is vital for plants to maintain nutrient balance and achieve optimal ***,we revealed a mechanism by which nitrate induces genes for phosphate utilization;this mechanism depends on NRT1.1B-facilitated degradation of cytoplasmic SPX4,which in turn promotes cytoplasmic-nuclear shuttling of PHR2,the central transcription factor of phosphate signaling,and triggers the nitrate-induced phosphate response(NIPR)and N-P coordinated utilization in *** this study,we unveiled a fine-tuning mechanism of NIPR in the nucleus regulated by Highly Induced by Nitrate Gene 1(HINGE1,also known as Rli1),a MYB-transcription factor closely related to ***1/HINGE1,which is transcriptionally activated by PHR2 under nitrate induction,can directly activate the expression of phosphate starvation-induced *** importantly,Rli1/HINGE1 competes with PHR2 for binding to its repressor proteins in the nucleus(SPX proteins),and consequently releases PHR2 to further enhance phosphate ***,Rli1/HINGE1 amplifies the phosphate response in the nucleus downstream of the cytoplasmic SPX4-PHR2 cascade,thereby enabling fine-tuning of N-P balance when nitrate supply is sufficient.

关键词： rice NIPR Rli1/HINGE1 PHR2 SPXs nucleus

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The effects of sowing date on cottonseed properties at different fruiting-branch positions

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Journal of Integrative Agriculture 2017年第6期16卷 1322-1330页

作者： hu Wei CHEN Mei-li ZHAO Wen-qing CHEN Bing-lin wang You-hua wang Shan-shan MENG Ya-li ZHOU Zhi-guo Key Laboratory of Crop Growth Regulation Ministry of Agriculture/College of Agriculture Nanjing Agricultural University Nanjing 210095 P.R.China

A two-year field experiment was conducted to illustrate the effects of sowing date on cottonseed properties at different fruiting-branch positions（FBPs）.Two cotton cultivars（Kemian 1 and Sumian 15）were sowed on 25 April,25 May,and10 June in 2010 and 2011,*** boll maturation period increased with the delaying of sowing *** sowing treatment（25 April）had higher seed weight,embryo weight,embryo oil content and protein content than late sowing treatments（25 May and 10 June）.The flowering date,seed weight,embryo weight,embryo oil and protein contents,and the dynamic changes of embryo oil and protein contents were altered by different FBPs.A significant interaction of sowing date×FBP was observed on embryo weight,embryo oil content,embryo protein content and the dynamic changes of embryo oil and protein contents,but was not observed on seed *** weight,embryo weight,embryo oil and protein content had significant positive correlations with the mean daily temperature（MDT）,mean daily maximum temperature（MDTmax）,mean daily minimum temperature（MDTmin）,and mean daily solar radiation（MDSR）,indicating that temperature and light resources were the main reasons for different sowing dates affecting the cottonseed properties at different ***,the difference in MDT was the main difference in climatic factors among different sowing dates.

关键词： sowing date fruiting-branch position cottonseed properties

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MicroRNAs Involved in the Pathogenesis of Phytophthora Root Rot of Soybean (Glycine max)

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agricultural sciences in china 2011年第8期10卷 1159-1167页

作者： wang Jing liU chun-yan ZHANG li-wei wang Jia-lin hu guo-hua DING Jun-jie CHEN Qing-shan college of Agriculture Northeast Agricultural UniversityHarbin 150030P.R.China Land Reclamation Research ＆ Breeding Centre Harbin 150090P.R.China Heilongjiang Academy of agricultural sciences Jiamusi Branch Jiamusi 154007P.R.China

Phytophthora root rot is one of the most prevalent diseases in the world,which can infect the seedlings and plants,with substantial negative impact on soybean yield and *** （miRNAs） are a class of post-transcriptional regulators of gene expression during growth and development of organisms.A soybean disease-resistance variety Suinong 10 was inoculated with Phytophthora sojae race No.1,and the specific miRNA resistant expression profile was acquired by microarray for the first *** expressional miRNAs have been found after comparing the results of the treated sample with the control ***,the target genes of different expressional miRNAs were *** miRNAs,cbr-mir-241 and ath-miR854a,regulated the disease-resistance process directly through their targets,some *** two miRNAs,gma-miR169a and ath-miR169h,participated in disease-resistance regulation as transcription ***,one miRNA,ptc-miR164f,has been reported to regulate the plant *** of these studies would be served as the foundation for exploring the resistance mechanism.

关键词： microRNA (miRNA) microarray soybean (Glycine max) gene expressional regulation

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Research and development of a novel subunit vaccine for the currently circulating pseudorabies virus variant in china

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Frontiers of agricultural science and Engineering 2015年第3期2卷 216-222页

作者： Yuzhou wang Tongyan wang He YAN Fanli YANG linghua guo Qingyuan YANG Xule hu Feifei TAN Yan XIAO Xiangdong li Kegong tian National Research Center for Veterinary Medicine Luoyang 471003China college of animal science and Veterinary Medicine Henan Agricultural UniversityZhengzhou 450002China

Pseudorabies(PR)is a devastating viral disease which leads to fatal encephalitis and respiratory disorders in *** gE-deleted live pseudorabies virus(PRV)vaccine has been widely used to control this disease in ***,the new-emerging variants of PRV compromises the protection provided by current vaccines and lead to the outbreak of PR in vaccinated pig *** killed and live vaccine candidates based on current PRV variants have been reported to be effective to control the disease.A subunit vaccine based on gB protein,one major PRV glycoprotein which elicits strong humoral and cellular immune responses,however,was never evaluated for protection against the current circulating PRV *** this study,full-length PRV gB protein was successfully expressed in baculovirus/insect cells in the soluble format and was tested on 3-week-old piglets as a subunit *** with unvaccinated pigs,the gB-vaccinated pigs developed specific antibody-mediated responses and were protected from the virulent PRV HN1201 *** vaccinated pigs survived without showing any PRV-specific respiratory and neurological signs,but all unvaccinated pigs died within 7 days after HN1201 ***,this novel gB-based vaccine could be applied as an effective subunit vaccine to control PRV variant in china.

关键词： pseudorabies virus glycoprotein B protein subunit vaccine

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Adsorptive property of Cu^(2+)-loaded montmorillonite clays for Escherichia coli K_(88) in vitro

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Journal of Environmental sciences 2011年第11期23卷 1808-1815页

作者： Tong guo Shoujun Cao Rui Su Zhiqiang li Ping hu Zirong Xu Beijing Vocational college of Agriculture Beijing 102442China college of animal science the Key Laboratory of Molecular animal Nutrition Ministry of Education Zhejiang university Hangzhou 310029China college of Chemical Engineer and Environment North University of ChinaTaiyuan 030051China college animal science and technology Institute of Grassland ScienceChina Agricultural UniversityBeijing 100093China

The adsorption properties of Cu 2＋ -loaded montmorillonite clays （MMT-Cu） for Escherichia coli K 88 as a function of time,bacteria concentrations,pH,ionic strength and temperature were *** results showed that the bacteria adsorption onto MMT-Cu surface reached equilibrium after 90 *** percentages of *** K 88 adsorbed onto the surfaces of MMT-Cu and montmorillonite clays （MMT） at equilibrium were 88.9% and 56.5%,*** electron microscopy revealed that a lot of *** K 88 adhered to the surface of *** zeta potential of MMT-Cu was relatively high as compared to that of *** adsorptive ability of MMT-Cu for *** K 88 was higher than that of MMT （P 0.05）.Moreover,pH,ionic strength and temperature produced a strong influence on the extent of *** K 88 adsorption to surface of MMT-Cu and *** mechanism of adsorption of *** onto MMT-Cu may involve electrostatic attraction and physiochemical properties of bacterial cell walls and minerals surfaces.

关键词： Cu2+-loaded montmorillonite clays adsorption Escherichia coli K88

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Preventing autosomal-dominant hearing loss in Bth mice with CRISPR/CasRx-based RNA editing

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Signal Transduction and Targeted Therapy 2022年第4期7卷 1258-1270页

作者： Ziwen Zheng guo li Chong Cui Fang wang Xiaohan wang Zhijiao Xu huiping guo Yuxin Chen Honghai Tang Daqi wang Mingqian huang Zheng-Yi Chen Xingxu huang huawei li Geng-lin li Xiaoxiang hu Yilai Shu ENT Institute and Department of Otorhinolaryngology Eye&ENT HospitalState Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain ScienceFudan UniversityShanghai 200031China Institutes of Biomedical sciences Fudan UniversityShanghai 200032China NHC Key Laboratory of Hearing Medicine(Fudan university) Shanghai 200032China State Key Laboratory of Agrobiotechnology China Agricultural UniversityBeijing 100193China college of Biological sciences China Agricultural UniversityBeijing 100193China School of life science and technology Southeast UniversityNanjing 210096China Department of Otolaryngology-Head and Neck Surgery Graduate Program in Speech and Hearing Bioscience and Technology and Program in NeuroscienceHarvard Medical SchoolBostonMA 02115USA Eaton-Peabody Laboratory Massachusetts Eye and Ear InfirmaryBostonMA 02114USA School of life science and technology ShanghaiTech UniversityShanghai 200031China CAS Center for Excellence in Molecular Cell science Shanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China Institutes of Brain science and the Collaborative Innovation Center for Brain science Fudan UniversityShanghai 200031China

CRISPR/RfxCas13d(CasRx)editing system can specifically and precisely cleave single-strand RNAs,which is a promising treatment for various disorders by downregulation of related gene ***,we tested this RNA-editing approach on Beethoven(Bth)mice,an animal model for human DFNA36 due to a point mutation in *** first screened 30 sgRNAs in cell cultures and found that CasRx with sgRNA3 reduced the Tmc1^(Bth)transcript by 90.8%,and the Tmc1 wild type transcript(Tmc1^(+))by 44.3%.We then injected a newly developed AAV vector(***)based CasRx into the inner ears of neonatal Bth mice,and we found that Tmc1^(Bth)was reduced by 70.2%in 2 weeks with few off-target effects in the whole ***,we found improved hair cell survival,rescued hair bundle degeneration,and reduced mechanoelectrical transduction ***,the hearing performance,measured in both ABR and DPOAE thresholds,was improved significantly in all ages over 8 ***,therefore,have validated the CRISPR/CasRx-based RNA editing strategy in treating autosomal-dominant hearing loss,paving way for its further application in many other hereditary diseases in hearing and beyond.

关键词： CRISPR/Cas editing hearing

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Migration of invasive Spodoptera frugiperda(Lepidoptera:Noctuidae)across the Bohai Sea in northern china

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Journal of Integrative Agriculture 2021年第3期20卷 685-693页

作者： JIA hui-ru guo Jiang-long WU Qiu-lin hu Chao-xing li Xiao-kang ZHOU Xian-yong WU Kong-ming State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China college of Plant Protection Shenyang Agricultural UniversityShenyang 110866P.R.China Guizhou Provincial Key Laboratory for agricultural Pest Management of the Mountainous Region Institute of EntomologyGuizhou UniversityGuiyang 550025P.R.China college of Plant Protection Southwest UniversityChongqing 400716P.R.China

The fall armyworm(FAW),Spodoptera frugiperda(***),is an important agricultural pest with a strong migratory *** the species is native to the Americas,it has recently invaded china,ravaging crops in many provinces(autonomous regions,municipalities).Its long-distance migration,which is critical to understand for pest management programs,has been well studied in its native *** other regions however,the moth’s migration patterns have not yet been ***,the migratory behavior of FAW populations in china was studied on an isolated island located in the center of the Bohai Strait from spring to autumn 2019,the year in which FAW first reached this region,by using searchlight trapping,stable carbon isotopes and trajectory *** main results were summarized as follows:(i)The number of FAW moths caught by searchlight trapping provided direct evidence that the species migrated across the Bohai Sea.(ii)Species identification was confirmed by both morphology and molecular methods,and only the"corn-strain"that preferentially infests maize and sorghum was found in the collections.(iii)Stable carbon isotope measurements showed that up to 95.07%of captured moths displayed the C4 isotope signature,thus providing additional evidence that this species is a migrant as there are no major C4 plants at the trapping site.(iv)Backward trajectory analysis indicated that the pest now threatens to expand its range into the agriculturally important region of northeast china,and thus authorities in this region should be vigilant to the threat posed by this ***,these findings add to our knowledge concerning the occurrence of FAW in northern china,and will help us to develop sustainable and effective monitoring,forecasting,and pest management strategies.

关键词： Spodoptera frugiperda overseas migration searchlight trapping host strains stable carbon isotopes migration trajectory

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Characterization of the petiole length in soybean compact architecture mutant M657 and the breeding of new lines

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Journal of Integrative Agriculture 2022年第9期21卷 2508-2520页

作者： GAO hua-wei SUN Ru-jian YANG Meng-yuan YAN Long hu Xian-zhong FU Guang-hui HONG hui-long guo Bing-fu ZHANG Xiang liU li-ke ZHANG Shu-zhen QIU li-juan Key Laboratory of Soybean Biology Ministry of Education/College of AgricultureNortheast Agricultural UniversityHarbin 150030P.R.China National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China hulun Buir Institution of agricultural sciences Zhalantun 162650P.R.China college of life science Liaocheng UniversityLiaocheng 252059P.R.China Institute of Cereal and Oil Crops Hebei Academy of Agricultural and Forestry Sciences/Hebei Laboratory of Crop Genetic and BreedingShijiazhuang 050035P.R.China Suzhou Academy of agricultural sciences Suzhou 234000P.R.China Crops Research Institute Jiangxi Academy of Agricultural Sciences/Nanchang Branch of National Center of Oil Crops Improvement/Jiangxi Province Key Laboratory of Oil Crops BiologyNanchang 330200P.R.China

Phenotypic screening of soybean germplasm suitable for high planting density is currently the most viable strategy to increase yield. Previous studies have shown that soybean varieties with dwarf features and a short petiole often exhibit a compact plant architecture which could improve yield through increased planting density, although previously reported short petiole accessions were ultimately not usable for breeding in practice. Here, we established a method to assess petiole length and identified an elite mutant line, M657, that exhibits high photosynthetic efficiency. The agronomic traits of M657 were evaluated under field conditions, and appeared to be stable for short petiole across seven locations in northern, huang–huai, and southern china from 2017 to 2018. Compared with the Jihuang 13 wild type, the mutant M657 was shorter in both petiole length and plant height, exhibited lower total area of leaf, seed weight per plant and 100-seed weight, but had an increased number of effective branches and the growth period was prolonged by 2–7 days. Using M657 as a parental line for crosses with four other elite lines, we obtained four lines with desirable plant architecture and yield traits, thus demonstrating the feasibility of adopting M657 in breeding programs for soybean cultivars of high density and high yield.

关键词： soybean plant architecture mutant petiole length breeding of new lines

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