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The Transfer and Change of Paints in a Hit-and-run Motor Vehicle Accident

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Journal of forensic science and Medicine 2024年第1期10卷 24-27页

作者： Can Hu Jun Zhu Hongcheng Mei hongling guo Ping Wang Yajun Li Trace Evidence Department Institute of Forensic ScienceMinistry of Public SecurityXichengBeijingChina

This work investigated transfer and change of paint evidences in a case of *** kinds of attachments were found on the clothes of the victim and they were initially considered paint fragments from the vehicle causing the *** spectroscopy(IR),scanning electron microscope-energy dispersive spectrometer,and Microspectrophotometry were applied for examination of paints and clothing *** was detected in one of the attachments and in the clothing fibers of the victim by IR.A traffic accident simulation experiment was designed and conducted to research whether the polyester attachments come from suspected vehicle paints or victim’s clothing *** results showed that a melt mixture of transferred paints and clothing fiber was formed after a violent *** the amount of transferred paints was too low to be detectable in the mixture,the components detected by IR were mainly from clothing ***,we inferred that only one kind of attachment and paint fragments existed on the clothes of the victim,and the polyester attachments cannot be used to indicate the composition of suspected vehicle *** fibers and paints are both common trace evidence in traffic accident cases,and more attention should be paid to the examination of transferred paints on clothing fibers.

关键词： Clothing fibers hit‑and‑run traffic accident infrared spectroscopy paints trace evidence transfer

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STR复合扩增技术在亲子鉴定中的应用

刑事技术 1999年第5期24卷 12-13页

作者：郭红玲赵鑫姜成涛周静公安部物证鉴定中心北京100038

采用12个荧光标记STR位点复合扩增,进行双亲亲子鉴定和单亲亲子鉴定,取得满意效果.

关键词：荧光标记 STR 复合扩增亲子鉴定

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鼠抗人转铁蛋白单链抗体基因序列测定及分析

刑事技术 2000年第6期25卷 7-9页

作者：李兆隆郭红玲常彩琴肖卫民涂政王明鑫王俭公安部物证鉴定中心北京100038

从携带人转铁蛋白单链抗体基因载体转化的大肠杆菌菌株中提取质粒,经电泳分析及筛选、纯化出线状质粒DNA,定量后以载体上人转铁蛋白单链抗体基因插入子两边的已知DNA序列经合成后作为引物进行测序扩增凝胶电泳,将得到的人转铁蛋白单链... 详细信息

从携带人转铁蛋白单链抗体基因载体转化的大肠杆菌菌株中提取质粒,经电泳分析及筛选、纯化出线状质粒DNA,定量后以载体上人转铁蛋白单链抗体基因插入子两边的已知DNA序列经合成后作为引物进行测序扩增凝胶电泳,将得到的人转铁蛋白单链杭体基因序列重轻链可变区部分同基因库中鼠抗体重轻链进行同源比对,证明人转铁蛋白单链抗体基因确由鼠抗体重轻链可变区基因构成,在此基础上,进一步对基因核苷酸序列的可变区结构特征作推断分析,并推导出其对应的氨基酸序列.

关键词：质粒提取 DNA测序同源比较基因结构分析

MS SQL Server数据库的取证检验

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刑事技术 2009年第2期34卷 27-29页

作者：李盛张国臣周冬林楚川红公安部物证鉴定中心北京100038

目的研究MS SQL Server数据库的取证方法。方法通过对MS SQL Server数据库存储结构的研究,以名为"sradmin"的数据库为实例,介绍了SQL Server数据库的提取、检验分析方法。结论本文提出的方法可以提取、检验分析SQL Server数据库,具有很... 详细信息

目的研究MS SQL Server数据库的取证方法。方法通过对MS SQL Server数据库存储结构的研究,以名为"sradmin"的数据库为实例,介绍了SQL Server数据库的提取、检验分析方法。结论本文提出的方法可以提取、检验分析SQL Server数据库,具有很强的实用性。

关键词：电子物证数据库 MS SQL Server

Highly sensitive nanozyme strip:An effective tool for forensic material evidence identification

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Nano Research 2024年第3期17卷 1785-1791页

作者： Juanji Hong Zhanjun guo Dihan Duan Yi Zhang Xin Chen Yongjiu Li Zheng Tu Lei Feng Lei Chen Xiyun Yan Lizeng Gao Minmin Liang Demin Duan Experimental Center of Advanced Materials School of Materials Science&EngineeringBeijing Institute of TechnologyBeijing 100081China College of Bioscience and Biotechnology Shenyang Agricultural UniversityShenyang 110866China Chongqing kangjuquanhong biology science and technology Co. ltdChongqing 400026China Key Laboratory of forensic Genetics Institute of Forensic ScienceMinistry of Public SecurityBeijing 100038China CAS Engineering Laboratory for Nanozyme Key Laboratory of BiomacromoleculesInstitute of BiophysicsChinese Academic of ScienceBeijing 100101China

During criminal case investigations,blood evidence tracing is critical for criminal ***,the blood stains are often cleaned or covered up after the crime,resulting in trace residue and difficult ***,a highly sensitive and specific method for the rapid detection of human blood stains remains *** solve this problem,we established a nanozyme-based strip for rapid detection of blood evidence with high sensitivity and *** construct reliable nanozyme strips,we synthesized CoFe_(2)O_(4) nanozymes with high peroxidase-like activity by scaling up to gram level,which can be supplied for six million tests,and conjugated antibody as a detection probe in nanozyme *** developed CoFe_(2)O_(4) nanozyme strip can detect human hemoglobin(HGB)at a concentration as low as 1 ng/mL,which is 100 times lower than the commercially available colloidal gold strips(100 ng/mL).Moreover,this CoFe_(2)O_(4) nanozyme strip showed high generality on 12 substrates and high specificity to human HGB among 13 animal blood ***,we applied the developed CoFe_(2)O_(4) nanozyme strip to successfully detect blood stains in three real cases,where the current commercial colloidal gold strip failed to *** results suggest that the CoFe_(2)O_(4) nanozyme strip can be used as an effective on-scene detection method for human blood stains,and can further be used as a long-term preserved material evidence for traceability inquiry.

关键词： CoFe_(2)O_(4)nanozyme nanozyme strip peroxidase-like activity human hemoglobin blood evidence

Discrimination of Car Headlight Plastic by Gel Permeation Chromatography

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Journal of forensic science and Medicine 2015年第1期1卷 43-47页

作者： Bo Wang Yangke Quan hongling guo china University of Political science and Law institute of forensic science Ministry of public SecurityBeijingChina

Car headlight plastic as a kind of evidence often can be seen in traffic accidents and some other *** tested 20 brands of car headlight plastic using gel *** data were processed using the Statistical Package for the Social sciences(SPSS)one‑way analysis of variance(ANOVA)and the discrimination rate was 97.14%.This indicated that we could discriminate between different headlights by the molecular weight of their headlight *** permeation chromatography is an effective method of discriminating between headlights,particularly in the case of a traffic accident.

关键词： Car headlights gel chromatography the weight average molecular weight

鼠抗人转铁蛋白抗体轻链与重链可变区基因库的制备

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刑事技术 2001年第3期26卷 8-10页

作者：李兆隆郭红玲常彩琴涂政肖卫民王明鑫王俭公安部物证鉴定中心北京100038

以纯化的人转铁蛋白免疫小鼠,测其血清效价,合乎要求后杀鼠取脾脏并以异硫氰酸胍和酚的混合溶液将其匀浆,再以氯仿抽提、异丙醇沉淀和乙醇洗涤,所得总RNA以oligo(dT)纤维素离心柱分离纯化制备mRNA,使mRNA达到cDNA合成所需的浓度后,反转... 详细信息

以纯化的人转铁蛋白免疫小鼠,测其血清效价,合乎要求后杀鼠取脾脏并以异硫氰酸胍和酚的混合溶液将其匀浆,再以氯仿抽提、异丙醇沉淀和乙醇洗涤,所得总RNA以oligo(dT)纤维素离心柱分离纯化制备mRNA,使mRNA达到cDNA合成所需的浓度后,反转录制备出cDNA,在合成的cDNA中加入轻、重链引物,扩增制备出小鼠抗人转铁蛋白抗体的轻、重链可变区基因库。

关键词：基因工程抗体制备免疫小鼠脾脏总RNA提取鼠抗体基因库抗人转铁蛋白抗体

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鼠抗人转铁蛋白单链抗体基因的构建

刑事技术 2001年第5期26卷 7-9页

作者：李兆隆郭红玲常彩琴涂政肖卫民王明鑫王俭公安部物证鉴定中心北京100038

目的为了得到可经载体连接表达、并只由抗体可变区构成的单链抗体基因而进行本实验。方法经扩增得到的鼠抗人转铁蛋白抗体轻、重链可变区基因经凝胶电泳分离、离心柱纯化及定量,同能柔性折叠的一段短连接肽基因一起进行扩增拼接反应,而... 详细信息

目的为了得到可经载体连接表达、并只由抗体可变区构成的单链抗体基因而进行本实验。方法经扩增得到的鼠抗人转铁蛋白抗体轻、重链可变区基因经凝胶电泳分离、离心柱纯化及定量,同能柔性折叠的一段短连接肽基因一起进行扩增拼接反应,而后在含限切酶位点的引物指导下扩增引入限切酶SfiI和NotI的酶切位点识别序列,产物以电泳鉴定、纯化和定量,再分别用SfiI和NotI酶切消化,最后再柱纯化。结果得到了携带可与载体连接的粘性末端的鼠抗人转铁蛋白单链抗体基因。结论两个不同的基因通过两端含互补序列的第三个基因片断可只经过扩增而无需DNA连接酶的作用而连接起来。对于抗体基因可由此而产生新的轻重链基因的组合,有可能产生性能更优异的抗体。

关键词：扩增拼接限切酶消化单链抗体 ScFv 鼠抗人转铁蛋白法医学

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鼠抗人转铁蛋白单链抗体基因的表达

刑事技术 2002年第1期27卷 3-6页

作者：李兆隆郭红玲常彩琴肖卫民涂政王明鑫王俭公安部物证鉴定中心北京100038

目的制备抗人转铁蛋白单链抗体。方法拼装好并带有酶切位点粘性末端的鼠抗人转铁蛋白的单链抗体基因经凝胶电泳定量后,同载体pCANTAB 5E连接,以其转化*** TG1细胞,经辅助噬菌体M13K07挽救构建噬菌体抗体表面呈现文库、抗原包被固相材... 详细信息

目的制备抗人转铁蛋白单链抗体。方法拼装好并带有酶切位点粘性末端的鼠抗人转铁蛋白的单链抗体基因经凝胶电泳定量后,同载体pCANTAB 5E连接,以其转化*** TG1细胞,经辅助噬菌体M13K07挽救构建噬菌体抗体表面呈现文库、抗原包被固相材料富集选择阳性重组噬菌体克隆（Panning）。感染能产生可溶抗体片段的大肠杆菌菌株*** HB2151,制备可溶抗体,以Anti-E末端抗体进行Western blot、ELISA检验和分子量测定。结果人转铁蛋白的单链抗体基因成功表达。结论用基因工程抗体技术制备抗体是可行的。

关键词：连接转化噬菌体抗体库基因表达可溶抗体鼠抗抗体蛋白单链抗体基因法医物证蛋白检验试剂