检索结果-南通市图书馆

Primary porcine hepatocytes with portal vein serum cultured on microcarriers or in spheroidal aggregates

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2000年第3期6卷 365-370页

作者： Gao Y Hu HZ Chen K Yang jz First Mil Med Univ Zhujiang Hosp Dept Hepatobiliary Surg Guangzhou 510282 Guangdong Provi Peoples R China

AIM To develop a culture mode providingdurable biomaterials with high yields andactivities used in bioartificial *** Hepatocytes were isolated from awhole pig liver by Seglen’s method of orthotopicperfusion with *** culture onmicrocarriers,primary porcine hepatocyteswere inoculated at a concentration of 5×10~7/mLinto the static culture systems containing 2 g/LCytodex-3,then supplemented with 100 mL/Lfetal calf serum(FCS)or 100 mL/L porcineportal vein serum(PPVS)*** aggregate culture hepatocytes wereinoculated into 100 mL siliconized flasks at aconcentration of 5.0×10~6/*** In culture on microcarriershepatocytes tended to aggregate on Cytodex-3obviously after being *** multi-cellular aggregated spheroids could be found inthe two systems 24 h-48 h after hepatocyteswere *** morphological charact-eristics and synthetic functions were maintainedfor 5 wk in FCS culture system and 8 wk in PPVSculture *** spheroidal aggregate cultureabout 80%-90% isolated hepatocytes becameaggregated spheroids 24h after cultured insuspension and mean diameter of the spheroidswas 100μ*** relationship among thehepatocytes resembled that in the liver in *** functions of albumin and urea of the spheroids were twice those of hepatocytescultured on *** As high-yields and high-activitymodes of culture on microcarriers or inspheroidal aggregate culture with portal veinserum are promising to provide biomaterials forbioartificial liver(BAL)efficiently.

关键词： porcine hepatocytes microcarriers cell culture spheroidal aggregate culture portal vein serum

Room-Temperature Synthesis of Fe-containing Mesoporous Silica

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1996年第11期7卷 1057-1058页

作者： Yuan, ZY Chen, TH Wang, jz Li, HX NANKAI UNIV DEPT CHEMTIANJIN 300071PEOPLES R CHINA

A Fe-containing mesoporous silica has been synthesized at room temperature using alkylamine as templating surfactant; XRD, IR, ESR and Si-29 MAS NMR spectra provided evidence of the presence of framework and non-frame... 详细信息

关键词： Fe Room-Temperature Synthesis of Fe-containing Mesoporous Silica

Cultivation of human liver cell lines with microcarriers acting as biological materials of bioartificial liver

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 1999年第3期5卷 221-224页

作者： Gao Y Xu XP Hu HZ Yang jz Mil Med Univ 1st Dept Gen Surg Zhujiang Hosp Guangzhou 510282 Guangdong Prov Peoples R China

AIM To improve the Cultivation efficiency and yield of human liver cell line *** High--density cultivation of CL--l onmicrocarriers was carried out with periodicobservation of their growth and *** specific functions of human liver cell werealso *** Cells of CL-1 cell line grew well onmicrocarrier Cytodex--3 and on the 7th day thepeak was reached. The amount of CLI cells was2.13 x 10’ and the total amount of albuminsynthesis reached 71 .23 ig, urea synthesis23 .32 mg and diazepam transformation 619 .7 ugrespectively. The yield of CL-l on microcarrierswas 49.3 times that of conventional *** amounts of albumin synthesis, ureasynthesis and diazepam transformation were39.8 times, 41 .6 times and 33.3 times those ofconventional Cultivation, *** The human liver cell line CL--1 canbe Cultivated to a high density with Cytodex--3and has better biological functions. High-densityCultivation of CLI on microcarriers can act asthe biological material of bioartificial liver.

关键词： cell Figure

Connective tissue growth factor induces transformation of renal fibroblasts into myofibroblasts

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2002年第9期47卷 741-744,793页

作者： Huang, HC Li, jz Wang, HY Peking Univ First Hosp Inst Nephrol Div Renal Beijing 100034 Peoples R China

Connective tissue growth factor (CTGF) is thought to be a specific mediator of TGF-β profibrogenic effect, but the role of CTGF in the transformation of renal fibroblasts to myofibroblasts, which are the most important host cells in the renal chronic fibrosis, is still unknown. Aimed at observing the biological effect of CTGF, we set up a cell line overexpressing CTGF, and analyzed the amount of myofibroblast and the level of extracellular matrix Collagen Ⅲ mRNA in cultured cells. Our results show that CTGF can directly induce myofibroblasts formation, and increase the level of Collagen Ⅲ mRNA. This suggests that CTGF may be a novel pharmacotherapeutical target protein for the interference with renal fibrosis, thus providing useful theoretic possibility for clinical prevention of chronic progression of kidney disease.

关键词： connective tissue growth factor fibroblasts myofibro-blast fibrosis.

Synthesis of A Novel Aluminosilicate with Bimodal Mesopore Distribution

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1997年第10期8卷 927-930页

作者： Yuan, ZY Wang, jz Li, HX Chang, ZX NANKAI UNIV DEPT CHEMCATALYSIS LABTIANJIN 300071PEOPLES R CHINA SINOPEC FUSHUN RES INST PETR & PETROCHEMFUSHUN 113001PEOPLES R CHINA

A novel aluminosilicate with well-defined bimodal mesopore distribution has been synthesized directly, in which one mesopore is distributed at around 3.8nm and the other at 2.6nm.

关键词： MSU Nature Synthesis of A Novel Aluminosilicate with Bimodal Mesopore Distribution SBA

Nitric oxide synthase distribution in esophageal mucosa and hemodynamic changes in rats with cirrhosis

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 1999年第3期5卷 213-216页

作者： Huang YQ Xiao SD Zhang DZ Mo jz Gen Hosp Benxi Iron & Steel Co Dept Gastroenterol Benxi 117000 Peoples R China Shanghai Second Med Univ Shanghai Inst Digest Dis Renji Hosp Shanghai 200001 Peoples R China

AIM To observe the nitric oxide synthase (NOS) distribution in the esophageal mucosa andhemodynamic changes in cirrhotic *** NOS distribution in the loweresophagus of rats with carbon tetrachlorideinduced cirrhosis was assessed by using NADPHdiaphorase (NADPH-d ) histochemical *** of NO in serum were measured byfluorometric assay. Mean arterial pressure(MAP), cardiac output (CO), cardiac index (CI),splanchnic vascular resistance (SVR ), andsplanchnic blood flow (SBF ) were alsodetermined using 5’CO-labeled *** Intensity of NOS staining in theesophageal epithelium of cirrhotic rats wassignificantly stronger than that in *** was a NOS--positive staining area in theendothelia of esophageal submucosal vessels ofcirrhotic rats, but the NOS staining was negativein normal rats. NO concentration of serum incirrhotic rats were significantly higher incomparison with that of controls. Cirrhotic ratshad significantly lower MAP, SVR and higherSBF than those of the *** SPlanchnic hyperdynamiccirculatory state was observed in rats withcirrhosis. The endogenous NO may play animportant role in development of esophagealvarices and in changes of hemodynamics incirrhosis.

关键词： NADPH Nitric oxide synthase distribution in esophageal mucosa and hemodynamic changes in rats with cirrhosis

Alcohol recognition based on twisted intramolecular charge transfer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1997年第22期42卷 1886-1889页

作者： Lu, jz Wei, SL Jiang, YB Xu, JG Xiamen Univ Dept Chem Res Lab SEDC Analyt Sci Mat & Life Chem Xiamen 361005 Peoples R China

TWISTED intramolecular charge transfer (TICT) is one of the hot points in condensed

关键词： TICT alcohol molecular recognition cyclodextrin.

Luminescence Properties of the Ternary Terbium Complexes with (Ortho and Para) Aminobenzoic acids and 1,10-Phenanthroline Incorporated in Silica Matrix by a Sol-gel Method

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1997年第7期8卷 633-636页

作者： Yan, B Zhang, HJ Wang, SB Ni, jz CHINESE ACAD SCI CHANGCHUN INST APPL CHEMLAB RARE EARTH CHEM & PHYSCHANGCHUN 130022PEOPLES R CHINA

Ternary complexes of terbium with ortho (and pam) aminobenzoic acid and 1,10-phenanthroline were introduced into silica gel by the sol-gel method. The luminescence behavior of the solid-state samples was studied durin... 详细信息

关键词： ABA Luminescence Properties of the Ternary Terbium Complexes with Aminobenzoic acids and 1,10-Phenanthroline Incorporated in Silica Matrix by a Sol-gel Method Ortho and Para

Experimental Study on the Armor Grate Plate of Sloping Breakwater Under the Action of Irregular Waves

在线全文

学校读者我要写书评

暂无评论

China Ocean Engineering 1997年第1期12卷 89-98页

作者： Wu, GQ Meng, XD Zhang, HY Zhang, jz OCEAN UNIV QINGDAO QINGDAO 266003PEOPLES R CHINA

The irregular wave experiment on the stability of the Grate Plate was carried out in the light of the wind wave spectrum recently advanced by Prof. Wen Shengchang. The stability formulas of GP under the action of irregular waves were procured. Comparisons between the formulas obtained and those of GP under regular waves advanced by the first author in 1993 showed a coincident result.

关键词： sloping breakwater grate plate irregular waves wave spectrum