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Comparison of Effective Components Contents of Lysimachia clethroides Duby with Three Extraction Methods

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Animal Husbandry and Feed Science 2015年第4期7卷 223-225,232页

作者： Wang Yanping guo shijin Dong Lin Xu Qianqian Zhang Ying Yang Limei Zhang Zhimei Ma Li Shen Zhiqiang Shandong Binzhou Animal Science and Veterinary Medicine Academy Shandong Engineering Technology Research Centre for Propolis Livestock and Poultry Vaccine Shandong Lvdu Ante Veterinary Drug Co. Ltd.

[ Objective] The differences in effective components contents of Lysimachia clethroides Duby were compared using three extraction methods. [ Method] L. clethroides was extracted by three methods, including water extraction, water extraction-alcohol precipitation and 75% ethanol extraction, to determine the effec- tive components of extracts, and contents of calcium, phosphorus and Vc were calculated. [ Result] L. clezhreides contained calcium salt, chlorine, VB1, VB2, Vc and phosphate, but did not contain citrate, sulfate, carbonate or bicarbonate, acetate, lactate, VA , VD and Vs ; the contents of calcium and phosphorus in water extract were 150 and 3.34 mg/g, respectively, greatly exceeding their contents in water extraction-alcohol precipitation and ethanol extraction, while the difference in Vc content was not significant. [ CondusionJ The paper provides a reference for practical application of L. clethroides as an additive.

关键词： Lysimachia clethroides Duby Water extraction Calcium Phosphorus Vc

Research Progress of Active Ingredients and Pharmacological Action of Honeysuckle

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Animal Husbandry and Feed Science 2014年第1期6卷 22-24页

作者： Zhang Zhimei guo shijin Fu Shijun Xu Qianqian Yang Limei Zhang Ying Wang Yanping Ma Li Wang Jianjun Shen Zhiqiang Shandong Binzhou Animal Science&Veterinary Medicine Institute Shandong Lvdu Ante Veterinary Drug Industry

Honeysuckle, the dry flower bud or with open flower of Lonicerajaponica in caprifoliaceae, has been touted as a cure-all for heat-clearing and detoxifying effect. Honeysuckle contains abundant components such as volatile oil, flavonoids and organic acids, playing the role of antibiosis, antivirus, antiphlogosis, immunopotenfiation and antioxidation. The active ingredients and pharmacological action of honeysuckle are reviewed in the paper, so as to lay the foundation for further application of honeysuckle in veterinary clinic.

关键词： Honeysuckle Active ingredients Pharmacological action

Effect of Chinese Herbal Medicine on Pathogenic Bacteria of Cow Mastitis

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Animal Husbandry and Feed Science 2020年第5期12卷 20-22页

作者： guo shijin Shandong Binzhou Animal Science and Veterinary Medicine Academy Binzhou 256600China Shandong Lvdu Ante Veterinary Drug Co. Ltd.Binzhou 256600China

[Objective]The paper was to analyze the clinical therapeutic effects of Chinese herbal medicine on pathogenic bacteria of cow mastitis.[Method]Eight Chinese herbs and three compound extracts were selected for the treatment of cow *** kinds of pathogenic bacteria were successfully isolated from cows with mastitis by flat perforation irrigation method and tube double dilution *** addition,in vitro antibacterial test was carried out,and in vitro inhibitory effects of single and compound Chinese medicine extracts on pathogenic bacteria causing cow mastitis were studied.[Result]A total of seven Chinese herbs had good inhibitory effect,such as Taraxacum mongolicum,Viola yedonensis and Polygala japonica,which received good effect of activating blood circulation and removing blood stasis,clearing away heat and toxic materials.[Conclusion]As good Chinese herbal medicines,***,*** and *** could be popularized.

关键词： Chinese herbal medicine Cow mastitis Pathogenic bacteria

熔融制样-X射线荧光光谱法测定环境土壤中主次组分

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冶金分析 2023年第4期43卷 77-83页

作者：于磊张晓郭诗锦王存璐国标(北京)检验认证有限公司北京100088

环境土壤中组成复杂,为了能够同时检测土壤中主次组分,建立了熔融制样-X射线荧光光谱法(XRF)测定环境土壤中主次组分的方法。为了防止腐蚀铂-金坩埚,实验先对环境土壤样品进行预氧化,即在800℃马弗炉灼烧2 h;再以四硼酸锂-偏硼酸锂混合... 详细信息

环境土壤中组成复杂,为了能够同时检测土壤中主次组分,建立了熔融制样-X射线荧光光谱法(XRF)测定环境土壤中主次组分的方法。为了防止腐蚀铂-金坩埚,实验先对环境土壤样品进行预氧化,即在800℃马弗炉灼烧2 h;再以四硼酸锂-偏硼酸锂混合熔剂(m(Li_(2)B_(4)O_(7))∶m(LiBO_(2))=67∶33)作为熔剂,确定稀释比为10∶1,以溴化锂为脱模剂,在1050℃熔融10 min,制得玻璃熔片。选择土壤系列套标绘制校准曲线,并采用经典系数法对校准曲线进行校正,建立环境土壤中SiO_(2)、TiO_(2)、Al_(2)O_(3)、Fe_(2)O_(3)、Mn_(3)O_(4)、MgO、CaO、Na_(2)O、K_(2)O、P_(2)O_(5)、SO_(3)、V_(2)O_(5)、Cr_(2)O_(3)、SrO、ZrO_(2)、BaO、NiO、CuO、ZnO、PbO、HfO_(2)等21种组分的校准曲线,各组分校准曲线的线性相关系数为0.99895~1.00000,各组分检出限为0.007%~0.023%。按照实验方法测定环境土壤样品中21种主次组分,结果的相对标准偏差(RSD,n=7)为0.055%~7.3%。按照实验方法测定3个环境土壤标准物质,各组分的测定值与认定值相吻合。

关键词：熔融制样 X射线荧光光谱法(XRF) 环境土壤主次组分

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一例鸭病毒性肝炎病原的分离鉴定

中国家禽 2007年第7期29卷 29-29,31页

作者：吴忆春苗立中郭时金山东省滨州职业学院生物工程系山东滨州256603 山东省滨州畜牧兽医研究院山东滨州256618

2007年1月，山东省临沂市某鸭场发生一起以鸭背脖为主要症状的传染病，用市售鸭病毒性肝炎卵黄及血清抗体配合抗生素，均不能有效治疗，特来我系畜禽诊断中心求诊。经流行病学、临床症状、剖检变化、实验室检查、病例复制试验等，确诊... 详细信息

2007年1月，山东省临沂市某鸭场发生一起以鸭背脖为主要症状的传染病，用市售鸭病毒性肝炎卵黄及血清抗体配合抗生素，均不能有效治疗，特来我系畜禽诊断中心求诊。经流行病学、临床症状、剖检变化、实验室检查、病例复制试验等，确诊该病为变异型雏鸭病毒性肝炎。

关键词：雏鸭病毒性肝炎分离鉴定病原山东省临沂市临床症状实验室检查血清抗体诊断中心

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基坑开挖与承压水突涌对桩基承载力影响分析

地下空间与工程学报 2024年第1期20卷 311-319页

作者：郭星宇冯世进上海勘察设计研究院(集团)有限公司上海200093 同济大学地下建筑与工程系上海200092

为研究基坑开挖与承压水突涌对桩基承载力的影响,选取典型工程案例,通过现场水位观测调查含水层分布情况及水位埋深,进行了坑底抗突涌验算,结合现场突涌发生过程,分析了突涌发生的原因。通过原位静力触探试验结果的对比,分析了坑底各土... 详细信息

为研究基坑开挖与承压水突涌对桩基承载力的影响,选取典型工程案例,通过现场水位观测调查含水层分布情况及水位埋深,进行了坑底抗突涌验算,结合现场突涌发生过程,分析了突涌发生的原因。通过原位静力触探试验结果的对比,分析了坑底各土层扰动度以及扰动度随深度的变化规律,并提出了扰动度与桩基设计参数的关系。结果发现:承压水击穿管桩土塞是引起突涌的主要原因;基坑开挖对坑底以下土体的影响深度约6.5 m~9 m,扰动度随深度线性递减;突涌对桩端以上约2 m范围土体强度影响最大,最大扰动度达到0.91,对桩端以下2 m~4 m深度范围土体影响线性递减;承压水突涌对桩基承载力影响远大于基坑开挖对桩基承载力的影响,突涌点位置桩基承载力受到的影响最大,距离突涌点越远影响越小。

关键词：基坑开挖承压水突涌扰动度桩基承载力

丹参川芎嗪注射液联合曲美他嗪治疗冠心病心绞痛的疗效观察

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中国现代医生 2014年第33期52卷 51-54页

作者：郭士金山东医学高等专科学校山东医专附属医院心内科山东临沂276000

目的探讨丹参川芎嗪注射液联合曲美他嗪治疗冠心病心绞痛的临床疗效。方法将冠心病心绞痛患者随机分为治疗组和对照组;治疗组在常规治疗后用丹参川芎嗪注射液联合曲美他嗪治疗。观察两组的临床疗效,心绞痛发作缓解及心电图改善情况,血脂... 详细信息

目的探讨丹参川芎嗪注射液联合曲美他嗪治疗冠心病心绞痛的临床疗效。方法将冠心病心绞痛患者随机分为治疗组和对照组;治疗组在常规治疗后用丹参川芎嗪注射液联合曲美他嗪治疗。观察两组的临床疗效,心绞痛发作缓解及心电图改善情况,血脂、C-反应蛋白变化情况。结果治疗组总有效率为93%,对照组总有效率为71%,治疗组缺血心电图改善总有效率为95%,对照组总有效率为76%,治疗组发作次数和硝酸甘油用量明显少于对照组,差异有统计学意义(P0.05),两组治疗后血压和心率比较无显著差异。结论丹参川芎嗪注射液联合曲美他嗪治疗冠心病心绞痛临床疗效显著。

关键词：曲美他嗪丹参川芎嗪注射液冠心病心绞痛

急性冠脉综合征与血清高尿酸关系的相关性研究

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中国现代医生 2007年第9S期45卷 31-32页

作者：郭士金山东医学高等专科学校诊断学教研室山东临沂276002

目的探讨急性冠脉综合征与血清尿酸的关系。方法应用酶联免疫吸附试验测定ACS组、稳定性心绞痛组、正常对照组血清尿酸水平,并进行统计学处理。结果血清尿酸水平:ACS组与稳定性心绞痛组、正常对照组的比较差异有统计学意义(P0.05)。结论... 详细信息

目的探讨急性冠脉综合征与血清尿酸的关系。方法应用酶联免疫吸附试验测定ACS组、稳定性心绞痛组、正常对照组血清尿酸水平,并进行统计学处理。结果血清尿酸水平:ACS组与稳定性心绞痛组、正常对照组的比较差异有统计学意义(P0.05)。结论ACS发生与血清尿酸水平存在密切关系。

关键词：急性冠脉综合征尿酸

Determination of Doxycycline in Doxycycline Hydrochloride Soluble Powder under Different Chromatographic Conditions

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Agricultural Biotechnology 2022年第5期11卷 121-129页

作者： Lanying guo Youjiang DIAO shijin guo Shandong Agriculture And Engineering University Jinan 250100China Shandong Binzhou Animal Science&Veterinary Medicine Academy Binzhou 256600China Shandong Lvdu Ante Animal Veterinary Drug Industry Binzhou 256600China

[Objectives]This study was conducted to compare the accuracy and detection speed of high performance liquid chromatography for determining the content of doxycycline hydrochloride in doxycycline hydrochloride soluble powder under different chromatographic conditions, in order to improve the level of laboratory testing. [Methods] Four sets of experiments were designed through a cross-test method. The contents of doxycycline hydrochloride in the raw materials of doxycycline hydrochloride and doxycycline hydrochloride soluble powder were determined by changing the chromatographic column and mobile phase conditions, and the feasibility and practicability of the four methods were judged by comparing the detection results through data and chromatographic signal processing. [Results] The content of doxycycline hydrochloride in finished doxycycline hydrochloride soluble powder and the raw materials of doxycycline hydrochloride could be accurately determined under the four chromatographic conditions, of which the experimental group using Agilent C18 column and the mobile chromatography conditions of finished doxycycline hydrochloride soluble powder had the shortest retention time. [Conclusions] The high performance liquid chromatography method using Agilent C18 chromatographic column and mobile phase chromatographic conditions of finished doxycycline hydrochloride soluble powder products can quickly and accurately determine the content of doxycycline hydrochloride in doxycycline hydrochloride soluble powder.

关键词： Doxycycline hydrochloride Chromatographic conditions High performance liquid chromatography