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Genome-wide SNP discovery and core marker sets for assessment of genetic variations in cultivated pumpkin(Cucurbita spp.)

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Horticulture Research 2020年第1期7卷 1139-1148页

作者： Nam Ngoc Nguyen Minkyung Kim Jin-Kee Jung Eun-Jo Shim Sang-Min Chung Younghoon Park gung pyo lee Sung-Chur Sim Department of Bioresources Engineering Sejong UniversitySeoul 05006South Korea Seed Testing and Research Center Korea Seed&Variety ServiceGimcheon 39660South Korea Department of Life Sciences Dongguk UniversitySeoul 04620South Korea Department of Horticultural Bioscience Pusan National UniversityMiryang 50463South Korea Department of Plant Science and Technology Chung-Ang UniversityAnsung 17546South Korea Plant Engineering Research Institute Sejong UniversitySeoul 05006South Korea

Three pumpkin species Cucurbita maxima,***,and *** are commonly cultivated *** identify genome-wide SNPs in these cultivated pumpkin species,we collected 48 F1 cultivars consisting of 40 intraspecific hybrids(15 ***,18 ***,and 7 ***)and 8 interspecific hybrids(*** x ***).Genotyping by sequencing identified a total of 37,869 confident SNPs in this *** SNPs were filtered to generate a subset of 400 SNPs based on polymorphism and genome *** the 400 SNPs,288 were used to genotype an additional 188 accessions(94 F1 cultivars,50 breeding lines,and 44 landraces)with a SNP array-based *** polymorphisms were observed in 224 SNPs(78.0%)and were used to assess genetic variations between and within the four predefined populations in 223 cultivated pumpkin *** principal component analysis and UPGMA clustering found four major clusters representing three pumpkin species and interspecific *** genetic differentiation was supported by pairwise Fst and Nei’s genetic *** interspecific hybrids showed a higher level of genetic diversity relative to the other three *** the 224 SNPs,five subsets of 192,96,48,24,and 12 markers were evaluated for variety *** 192,96,and 48 marker sets identified 204(91.5%),190(85.2%),and 141(63.2%)of the 223 accessions,respectively,while other subsets showed<25%of variety identification *** SNP markers provide a molecular tool with many applications for genetics and breeding in cultivated pumpkin.

关键词： breeding cultivar Cucurbita

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Berberine induces cell cycle arrest and apoptosis in human gastric carcinoma SNU-5 cell line

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World Journal of Gastroenterology 2006年第1期12卷 21-28页

作者： Jing-Pin Lin Jai-Sing Yang Jau-Hong lee Wen-Tsong Hsieh Jing-gung Chung School of Chinese Medicine China MedicalUniversity Taichung City 404 Taiwan China Department of Medical Technology Yuan-PeiUniversity of Science and Technology Hsinchu City 300 TaiwanChina Department of Surgery China Medical UniversityHospital Taichung City 404 Taiwan China Department of Pharmacology China MedicalUniversity Taichung City 404 Taiwan China Department of Microbiology School ofBiological Science and Technology China Medical UniversityTaichung City 404 Taiwan China

AIM： To investigate the relationship between the inhibited growth （cytotoxic activity） of berberine and apoptotic pathway with its molecular mechanism of action. METHODS： The in vitro cytotoxic techniques were complemented by cell cycle analysis and determination of sub-G1 for apoptosis in human gastric carcinoma SNU-5 cells. Percentage of viable cells, cell cycle, and sub431 group （apoptosis） were examined and determined by the flow cytometric methods. The associated proteins for cell cycle arrest and apoptosis were examined by Western blotting. RESULTS： For SNU-5 cell line, the IC （50） was found to be 48 μmol/L of berberine. In SNU-5 cells treated with 25-200 μmol/L berberine, G2/M cell cycle arrest was observed which was associated with a marked increment of the expression of p53, Wee1 and CDk1 proteins and decreased cyclin B. A concentration-dependent decrease of cells in G0/G1 phase and an increase in G2/M phase were detected. In addition, apoptosis detected as sub-Go cell population in cell cycle measurement was proved in 25-200 μmol/L berberine-treated cells by monitoring the apoptotic pathway. Apoptosis was identified by sub-Go cell population, and upregulation of Bax, downregulation of Bcl-2, release of Ca^2＋, decreased the mitochondrial membrane potential and then led to the release of mitochondrial cytochrome C into the cytoplasm and caused the activation of caspase-3, and finally led to the occurrence of apoptosis. CONCLUSION： Berberine induces p53 expression and leads to the decrease of the mitochondrial membrane potential, Cytochrome C release and activation of caspase-3 for the induction of apoptosis.

关键词： Berberine Cell cycle Apoptosis Caspase-3 ROS MMP SNU-5 cells

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Pancreatic stent improves the success rate of needle-knife papillotomy in patients with difficult biliary cannulation

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World Journal of Gastroenterology 2025年第1期31卷 58-67页

作者： Mu-Hsien lee Cheng-Hui Lin Chi-Huan Wu Yung-Kuan Tsou Kai-Feng Sung Sheng-Fu Wang Nai-Jen Liu Department of Gastroenterology and Hepatology Chang Gung Memorial Hospital and Chang Gung University College of MedicineTaoyuan 33305Taiwan School of Medicine Chang Gung UniversityTaoyuan 33305Taiwan

BACKGROUND Needle-knife precut papillotomy(NKP)is typically performed ***,it remains unclear whether pancreatic stent(PS)placement can improve the outcomes of *** To explore whether PS placement improves the success rate of NKP in patients with difficult biliary *** This single-center retrospective study included 190 patients who underwent NKP between January 2017 and December 2021 after failed conventional biliary *** cases with incidental pancreatic duct cannulation during conventional biliary cannulation,the decision for pre-NKP PS placement was made at the endoscopist's *** primary outcome was the difference in the NKP success rate between patients with and without PS placement;the secondary outcome was the adverse event *** Among the 190 participants,82 received pre-NKP PS(PS-NKP group)whereas 108 did not[freehand or freehand NKP(FH-NKP)group].Post-NKP selective biliary cannulation was successful in 167(87.9%)patients,and the PS-NKP had a significantly higher success rate than the FH-NKP group(93.9%vs 83.3%,P=0.027).The overall adverse event rates were 7.3%and 11.1%in the PS-NKP and FH-NKP groups,respectively(P=0.493).A periampullary diverticulum(PAD)and significant intraoperative bleeding during NKP were independently associated with NKP failure;however,a pre-NKP PS was the only predictor of NKP *** the 44 participants with PADs,the PS-NKP group had a non-significantly higher NKP success rate than the FH-NKP group(87.5%and 65%,respectively;P=0.076).CONCLUSION PS significantly improved the success rate of NKP in patients with difficult biliary cannulation.

关键词： Difficult biliary cannulation Endoscopic retrograde cholangiopancreatography Needle-knife papillotomy Pancreatic stent Selective biliary cannulation

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Improving the Complexity of Turbo Code Decoding

Improving the Complexity of Turbo Code Decoding

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2002海峡两岸三地无线科技研讨会

作者： Erl-Huei Lu, Yeun-Renn Ting, Jau-Yien lee Department of Electrical Engineering, Chang gung University, Tao-Yuan, Taiwan, China

This work presents a fast maximum a posteriori (MAP) decoding algorithm based on a modified BGT algorithm [1]. Since the probability function of the proposed algorithm has been simplified, the computa

关键词： turbo code MAP decoding algorithm computational complexity

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Phyllanthus urinaria triggers the apoptosis and Bcl-2 down-regulation in Lewis lung carcinoma cells

Phyllanthus urinaria triggers the apoptosis and Bcl-2 down-r...

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海峡两岸中西医结合学术研讨会

作者： Sheng-Teng Huang, Rong-Chi Yang, Li-Jiun Yang, Pei-Nir lee, Jong-Hwei S.Pang Graduate Institute of Clinical Medical Sciences, Chang gung University, Tao-Yuan, Taiwan Chinese Herbal Pharmacy, Chang gung Memorial Hospital, Tao-Yuan, Taiwan Department of Chinese Medicine, Chang gung Memorial Hospital, Kaohsiung, Taiwan

Aim: Phyllanthus urinaria(P. urinaria), a widely used herb medicine, was tested for its anticancer effectthrough apoptotic process in vitro for the first time. Methods: We used the MTT assay and flowcytometry to determined the cell viability after P. urinariatreatment. Cell morphology, DNA ladder and caspase 3 were applied to evaluate the apoptotic change with ***. RNA isolation and RT-PCR were introduced to this panel to assess the apoptotic gene expressionsuch as Fas receptor/ligand, Bax, Bcl-2, p53 and p21.

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Efficient Hardware Design for Turbo Code Encoders

Efficient Hardware Design for Turbo Code Encoders

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2002海峡两岸三地无线科技研讨会

作者： Erl-Huei Lu, Yeun-Renn Ting, Jau-Yien lee, senior member, IEEE Department of Electrical Engineering, Chang gung University, Tao-Yuan, Taiwan, China

This investigation proposed an efficient circuit of turbo code encoder. The total propagation delay of a recursive convolutional encoder (RCE) in the present turbo encoder is only half as long as that

关键词： turbo codes encoding digital circuits

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Electroporative interleukin-10 gene transfer ameliorates carbon tetrachlo-ride-induced murine liver fibrosis by MMP and TIMP modulation

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Acta Pharmacologica Sinica 2006年第4期27卷 469-476页

作者： Tsung-hsing lee Allan M CONCEJERO Departments of Anesthesiology Departments of Surgery Kaohsiung Chang Gung Memorial HospitalTaipeiChina

Aim:Liver fibrosis represents a process of healing and scarring in response tochronic liver *** therapies for liver fibrosis are ***-10（IL-10）is a cytokine that downregulates pro-inflarnmatory responses and has amodulatory effect on hepatic *** aim of this study was to investi-gate whether electroporative IL-10 gene therapy has an hepatic fibrolytic effect ***:Hepatic fibrosis was induced by administering carbon tetrachlo-ride（CCl4）for 10 weeks in *** human IL-10 expression plasmid was deliv-ered via electroporation after hepatic fibrosis was ***,reverse transcription polymerase chain reaction（RT-PCR）,immunoblotting,andgelatin zymography were used to investigate the possible mechanisms of actionof ***:Human IL-10 gene therapy reversed CCl4-induced liver fibrosisin ***-PCR revealed that IL-10 gene therapy attenuated liver TGF-β1,col-lagen α1,fibronectin,and cell adhesion molecule mRNA *** transfer,both the activation of(x-smooth muscle actin and cyclooxygenase-2were significantly ***,IL-10 significantly inhibited matrixmetalloproteinase-2（MMP-2）and tissue inhibitors of matrix metalloproteinase（TIMP）activation after ***:We demonstrated that IL-10gene therapy attenuated CCl4-induced liver fibrosis in ***-10 preventedupregulated fibrogenic and pro-inflammatory gene *** collagenolyticeffect may be attributed to MMP and TIMP ***-10 gene therapy maybe an effective therapeutic modality against liver fibrosis with potential clinicaluse.

关键词： interleukin-10 gene therapy liver cirrhosis gelatinase A tissue inhibitor of metalloproteinases cyclooxygenase 2

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Double balloon endoscopy increases the ERCP success rate in patients with a history of Billroth Ⅱ gastrectomy

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World Journal of Gastroenterology 2010年第36期16卷 4594-4598页

作者： Jui-Hsiang Tang Yung-Kuan Tsou Hao-Tsai Cheng Mu-Hsien lee Ching-Song lee Nai-Jen Liu Division of Digestive Therapeutic Endoscopy Department of Gastroenterology and HepatologyChang Gung Memorial Hospital College of Medicine Chang Gung University

AIM:To evaluate the effect of double balloon endoscope(DBE)on the endoscopic retrograde cholangio-pancreatography(ERCP)success rate in patients with a history of BillrothⅡ(BⅡ)***:From April 2006 to March 2007,32 patients with a BⅡgastrectomy underwent 34 ERCP *** all cases,the ERCP procedures were started using a *** intubation of the afferent loop or reaching the papilla failed,we changed to DBE for the ERCP procedure(DBE-ERCP).We assessed the success rate of afferent loop intubation,reaching the major papilla,selective cannulation,possibility of therapeutic approaches,procedure-related complications,and the overall success ***:Among the 32 patients with a history of BⅡgastrectomy,the duodenoscope was successfully passed up to the papilla in 22 patients(69%),and cannulation was successfully performed in 20 patients(63%).Six patients(2 with failure in afferent loop intubation and 4 with failure in reaching the papilla)underwent *** DBE reached the papilla in all the 6 patients(100%)and selective cannulation was successful in 5 patients(83%).Four patients(67%)who had common bile duct stones were successfully *** patient underwent diagnostic ERCP only and the other one,in whom selective cannulation failed,was diagnosed with papilla cancer proven by *** were no complications related to the *** overall ERCP success rate increased to 88%(28/32).CONCLUSION:The overall ERCP success rate increases with DBE in patients with a previous BⅡgastrectomy.

关键词： Double balloon endoscopy Endoscopic retrograde cholangiopancreatography BillrothⅡgastrectomy

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Increased extrinsic apoptotic pathway activity in patients with hepatocellular carcinoma following transarterial embolization

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World Journal of Gastroenterology 2011年第42期17卷 4675-4681页

作者： Shih-Ho Wang Li-Mien Chen Jane-Dar lee Division of General Surgery Department of SurgeryKaohsiung Chang Gung Memorial Hospital and Chang Gung UniversityCollege of Medicine Division of Hematology/Oncology Department of MedicineTaichung Armed Forces General Hospital Central Taiwan University of Science and Technology Department of Surgery Taic-hung Armed Forces General Hospital

AIM： TO determine the apoptosis pathway in residual viable hepatocellular carcinoma （HCC） tissues following transarterial embolization （TAE）.andα fetoprotein （AFP） levels after TAE. All tissue samples were taken from the residual tumors. The expression of various apoptotic proteins was evaluated via immunoblotting procedures. The results were analyzed using a Student＇s t test. RESULTS： Tumor size and the AFP level decreased by 46.2% and 55.3% after TAE, respectively. There was no significant difference detected for the Bcl-2/Bax ratio or the cleaved caspase-9 expression levels in either group. However, extrinsic apoptopic pathway-related expression of Fas and cleaved caspase-8 expression were significantly higher in the study group than in the control group （P 〈 0.05）. In addition, cleaved caspase-3 expression in the study group was higher （1.62-fold） than in control group （P 〈 0.05）. CONCLUSION： TAE is an effective palliative therapy that decreases tumor size and AFP levels via an increase in extrinsic apoptosis pathway in patients with unresectable HCC.

关键词： Apoptosis Hepatocellular carcinoma Transarterial embolization

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Efficacy of surgical resection in management of isolated extrahepatic metastases of hepatocellular carcinoma

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World Journal of Gastroenterology 2009年第43期15卷 5481-5488页

作者： Kun-Ming Chan Ming-Chin Yu Ting-Jung Wu Chen-Fang lee Tse-Ching Chen Wei-Chen lee Miin-Fu Chen Department of General Surgery Chang Gung Memorial Hospital-Linkou Medical Center Chang Gung University College of Medicine 33305 Taoyuan Taiwan China Department of Pathology Chang Gung Memorial Hospital-Linkou Medical Center Chang Gung University College of Medicine 33305 Taoyuan Taiwan China

AIM:To clarify the benefit of surgical excision for patients with extrahepatic metastases of hepatocellular carcinoma(HCC). METHODS:We retrospectively reviewed the medical records of 140 patients with pathologically proven extrahepatic metastases of HCC and evaluated the outcomes of those who had undergone surgical resection(SR)for extrahepatic metastatic *** made on the basis of extrahepatic metastatic sites were also examined. RESULTS:The survival rates of patients who underwent SR of extrahepatic metastases were significantly better than those of patients who did not receive SR. For the SR group,1-and 3-year survival rates were 24%and 7%,respectively,while for the non-resection group,the survival rates were 8%and 0%,respec- tively(P<0.0001).Survival rates related to metastatic sites were also significantly superior after SR of extrahepatic metastases:median survivals were 32 mo with lung metastasis,10 mo with bone metastasis,6.1 mo with brain metastasis. CONCLUSION:SR can provide survival benefits forpatients with 1 or 2 isolated extrahepatic metastases and who concurrently exhibit good hepatic functional reserve and general performance status as well as successful treatment of intrahepatic HCC.

关键词： Hepatocellular carcinoma Hepatic resection Extrahepatic metastases, Surgical resection Outcome

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