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Nuclear-targeted therapy of nerve growth factor conjugated with ^(125)I for human glioma U251 cells

Neural Regeneration Research 2011年第34期6卷 2694-2702页

作者： Yun Feng Rongguang Shao Yong Yang Hongwei He Xiaofang Li gulshan elahi Department of Pharmacology School of Medical Science and Laboratory Medicine Jiangsu University Zhenjiang 212013 Jiangsu Province China Institute of Medicinal Biotechnology Peking Union Medical College Chinese Academy of Medical Sciences Beijing 100050 China

The present study investigated the nuclear transportation phenomenon of ^125I-nerve growth factor （NGF） and the DNA-damaging changes to U251 cells using microautoradiography and single cell electrophoresis. The results showed that ^125I-NGF inhibited the survival of p53 mutant U251 human glioma cell/tumor and enhanced the therapeutic effectiveness of vincristine in in vivo and in vitro models. In vitro experiments showed ^125I-NGF was transported into the nucleus and damaged the DNA in U251 cells. Moreover, ^125I-NGF locked the U251 cells in the G2 phase. Further investigation showed that ^125I-NGF decreased cyclin B1 protein levels in a dose dependent manner, but the level of cyclin B1 mRNA expression remained unchanged. ^125I-NGF increased phosphorylated Chkl, Chk2 and Cdc25c protein levels in U251 cells, but did not influence p53 and p21 protein expression. Moreover, ^125I-NGF and vincristine exhibited synergistic effects on reducing cyclin B1 protein levels. These results indicate that ^125I-NGF can provide anti-tumor effects by activating the ATM and ATR pathways through DNA damage.

关键词： nuclear-targeted therapy nerve growth factor ^125I glioma conjugate cell cycle arrest

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神经生长因子^(125)I偶联物诱导人胶质瘤U251细胞G_2期阻滞

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科技导报 2012年第12期30卷 37-41页

作者：封云陈曦梁炜杨勇 gulshan elahi 江苏大学基础医学与医学技术学院药理系江苏镇江212013

观察125I-NGF(神经生长因子)对U251细胞DNA的损伤作用,探讨125I-NGF的抗肿瘤作用机制。应用微核测试和单细胞电泳等方法观察U251细胞DNA的变化,WesternBlotting法检测细胞蛋白水平的变化,RT-PCR法检测CyclinB1mRNA水平的变化,微核测试... 详细信息

观察125I-NGF(神经生长因子)对U251细胞DNA的损伤作用,探讨125I-NGF的抗肿瘤作用机制。应用微核测试和单细胞电泳等方法观察U251细胞DNA的变化,WesternBlotting法检测细胞蛋白水平的变化,RT-PCR法检测CyclinB1mRNA水平的变化,微核测试和单细胞电泳等方法观察到125I-NGF对U251细胞DNA的损伤作用。细胞周期检测及MPM-2/PI复染实验表明125I-NGF诱导了U251细胞G2期阻滞。WesternBlotting检测表明,125I-NGF可能通过活化ATM和ATR途径发挥抗肿瘤作用。125I-NGF以剂量依赖性的模式减少了U251细胞CyclinB1蛋白表达水平,但没有改变其mRNA的表达水平。在U251细胞中,磷酸化Chk1、Chk2和Cdc25c蛋白水平提高,而p53和p21水平保持不变。阐明了125I-NGF通过对U251细胞DNA的损伤作用活化ATM和ATR通路,使多种蛋白的表达水平发生了改变,从而发挥其抗肿瘤的作用。

关键词：神经生长因子 125I 胶质瘤偶联物细胞周期阻滞

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Diet and feeding ecology of black-barred halfbeak Hemiramphus far(Forsskal, 1775) (Hemiramphidae) from Karachi coast of Pakistan

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Journal of Coastal Life Medicine 2017年第1期5卷 4-6页

作者： Sadaf Tabassum Farzana Yousuf Naeema elahi MdYeamin Hossain MdAlomgir Hossen Fairuz Nawer Dalia Khatun MostFaridaParvin Department of Zoology Federal Urdu University of ArtsScience and TechnologyGulshan-e-Iqbal CampusKarachi75300Pakistan Department of Zoology University of KarachiKarachi75270Pakistan Department of Fisheries Faculty of AgricultureUniversity of RajshahiRajshahi6205Bangladesh

Objective:To describe the diet and feeding ecology ofHemiramphus far (H. far) through investigating the stomach contents. Methods: Sampling of total 430 individuals ofH. far was carried out from commercial catches landed at West Wharf fish harbor of Karachi coast, Pakistan during January–December 2010. The total length and weight of each individual were recorded. Samples were parted into Group 1 (15–20 cm,n = 240) and Group 2 (21–26 cm,n = 190), to estimate changes in diet with fish size. To remove alimentary canal, fishes were dissected and kept into 10% formalin. Then gut contents were examined under a light microscope for each intestine. Stomach fullness as well as dietary composition was ascertained from gut contents. Examination and analyses were conducted using frequency of occurrence and numerical method. Results:The examination confirmed 47.67% stomach as empty. Furthermore, our study revealed that the diet ofH. far consists of fishes, isopods, ostracods,Daphnia, shells, algae, unidentified plants and animal materials. This study proved thatH. far is an omnivorous fish feeding on fishes, isopods, shells, algae and plant and animal originated different food items. According to the Schoener overlap index, there was no significant difference in feeding of the two length groups ofH. far (C = 0.833). In addition, the Shannon-Wiener index presented that in Group 1, the prey diversity of the halfbeak was lower (H = 0.90) than that of Group 2 (H = 1.02). Conclusions: The findings of the present study would be very effective for sustainable management of black-barred halfbeak fishery in the Karachi coast of Pakistan and the surrounding ecosystems.

关键词： Pakistan Karachi coast Feeding ecology Stomach contents Hemiramphus far

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