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Genes & Diseases 2024年第3期11卷 206-219页

作者： Shuyu Li Zichen Xu Yi wang Lizhao Chen Xiangxiu wang Yanghao Zhou Daoxi Lei Guangchao Zang guixue wang Key Laboratory of Biorheological Science and Technology of Ministry of Education National and Local Joint Engineering Laboratory for Vascular ImplantsBioengineering College of Chongqing UniversityChongqing 400030China College of Basic Medical Sciences Chongqing Medical UniversityChongqing 400016China Department of Neurosurgery Daping HospitalArmy Medical Center of PLAArmy Medical UniversityChongqing 400042China

Atherosclerotic cardiovascular disease and its complications are a high-incidence disease *** studies have shown that blood flow shear has a huge impact on the function of vascular endothelial cells,and it plays an important role in gene regulation of pro-inflammatory,pro-thrombotic,pro-oxidative stress,and cell *** impor-tant endothelial cell mechanosensitive genes have been discovered,including KLK10,CCN gene family,NRP2,YAP,TAZ,HIF-1α,NF-kB,FOS,JUN,TFEB,KLF2/KLF4,NRF2,and *** of them have been intensively studied,whereas the relevant regulatory mechanism of other genes remains *** on these mechanosensitive genes will provide new strategies for therapeutic intervention in atherosclerotic vascular ***,this article reviews the mechanosensitive genes affecting vascular endothelial cells,including classical pathways and some newly screened genes,and summarizes the latest research progress on their roles in the pathogenesis of atherosclerosis to reveal effective therapeutic targets of drugs and provide new insights foranti-atherosclerosis.

关键词： Atherosclerosis Blood flow Endothelial cell Mechanosensitive gene Therapeutic target

A Novel Poly(3-hexylthiophene) Engineered Interface for Electrochemical Monitoring of Ascorbic Acid During the Occurrence of Glutamate-Induced Brain Cytotoxic Edemas

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Research 2024年第1期2023卷 301-313页

作者： Zexuan Meng Yuchan Zhang Lu Yang Shuang Zhao Qiang Zhou Jiajia Chen Jiuxi Sui Jian wang Lizhong Guo Luyue Chang Jialing He guixue wang Guangchao Zang Institute of Life Science and Laboratory of Tissue and Cell BiologyLab Teaching and Management CenterChongqing Medical UniversityChongqing 400016China Key Laboratory for Biorheological Science and Technology of Ministry of Education State and Local Joint Engineering Laboratory for Vascular ImplantsBioengineering College of Chongqing UniversityChongqing 400030China Jinfeng Laboratory Chongqing 401329China Department of Pathophysiology Chongqing Medical UniversityChongqingChina

Although neuroelectrochemical sensing technology offers unique benefits for neuroscience research,its application is limited by substantial interference in complex brain environments while ensuring biosafety *** this study,we introduced poly(3-hexylthiophene)(P3HT)and nitrogen-doped multiwalled carbon nanotubes(N-MWCNTs)to construct a composite membrane-modified carbon fiber microelectrode(CFME/P3HT-N-MWCNTs)for ascorbic acid(AA)*** microelectrode presented good linearity,selectivity,stability,antifouling,and biocompatibility and exhibited great performance for application in neuroelectrochemical ***,we applied CFME/P3HT-N-MWCNTs to monitor AA release from in vitro nerve cells,ex vivo brain slices,and in vivo living rat brains and determined that glutamate can induce cell edema and AA *** also found that glutamate activated the N-methyl-d-aspartic acid receptor,which enhanced Na^(+) and Cl^(−) inflow to induce osmotic stress,resulting in cytotoxic edema and ultimately AA *** study is the first to observe the process of glutamate-induced brain cytotoxic edema with AA release and to reveal the *** work can benefit the application of P3HT in in vivo implant microelectrode construction to monitor neurochemicals,understand the molecular basis of nervous system diseases,and discover certain biomarkers of brain diseases.

关键词： release microelectrode Engine

The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling

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Research 2024年第2期2023卷 659-673页

作者： Xi Chen Zichen Xu Kai Tang Guanshuo Hu Pengyu Du Junfang wang Cunyu Zhang Ying Xin Keming Li Qiantang Zhang Jianjun Hu Zhuxue Zhang Mo Yang guixue wang Youhua Tan The Hong Kong Polytechnic University Shenzhen Research Institute Shenzhen518057China Key Laboratory for Biorheological Science and Technology of Ministry of Education State and Local Joint Engineering Laboratory for Vascular ImplantsBioengineering College of Chongqing UniversityChongqing400030China Research Institute of Smart Ageing The Hong Kong Polytechnic UniversityHong KongChina Department of Biomedical Engineering The Hong Kong Polytechnic UniversityHong KongChina Department of Pathology Guizhou Provincial People's HospitalGuiyangGuizhou550002China

Tumor cells progressively remodel cytoskeletal structures and reduce cellular stiffness during tumor progression,implicating the correlation between cell mechanics and ***,the roles of tumor cell cytoskeleton and the mechanics in tumor progression remain incompletely *** report that softening/stiffening tumor cells by targeting actomyosin promotes/suppresses self-renewal in vitro and tumorigenic potential in ***/strengthening actin cytoskeleton impairs/reinforces the interaction between adenomatous polyposis coli(APC)andβ-catenin,which facilitatesβ-catenin nuclear/cytoplasmic ***β-catenin binds to the promoter of Oct4,which enhances its transcription that is crucial in sustaining self-renewal and *** results demonstrate that the mechanics of tumor cells dictate self-renewal through cytoskeleton-APC-Wnt/β-catenin-Oct4 signaling,which are correlated with tumor differentiation and patient *** study unveils an uncovered regulatory role of cell mechanics in self-renewal and malignancy,and identifies tumor cell mechanics as a hallmark not only for cancer diagnosis but also for mechanotargeting.

关键词： diagnosis skeleton APC

Effects of COVID-19 pandemic on human fertility:A scientometric and visualized evaluation

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Genes & Diseases 2024年第4期11卷 113-115页

作者： Xiangxiu wang Yifan Rao Lili Tan Ziqiu Hu Lin Wen Weixi Qin Bingyi Li Keqiao He Tieying Yin Juhui Qiu Yuming wang Yaokai Chen Xiancai Rao guixue wang Key Laboratory of Biorheological and Technology of the Ministry of Education State and Local Joint Engineering LaboratoryforVascular ImplantsModern LifeScience Experiment Teaching Center at Bioengineering College of Chongqing UniversityChongqing 400030China Department of Microbiology College of Basic Medical SciencesKey Laboratory of Microbial Engineering Under the Educational Committee in ChongqingArmy Medical University(Third Military Medical University)Chongqing 400038China Jinfeng Laboratory Chongqing 401329China Division of Infectious Diseases Chongqing Public Health Medical CenterChongqing 400036China

CovID-19,also known as coronavirus disease 2019,is a novel coronavirus disease with high infectivity,strong heterogeneity,and long incubation period(generally 3-14 days).Its main symptoms and signs include fever,dry cough,nasal congestion,fatigue,disorientation,lymphopenia,and *** short-term and long-term impacts of covID-19 on human health,particularly its effects on human reproduction and offspring development,continue to receive significant concerns,as they may lead to potential sequelae for several decades or even centuries.

关键词： nasal fertility fever

Coupled single-cell and bulk RNA-seq analysis reveals the engulfment role of endothelial cells in atherosclerosis

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Genes & Diseases 2024年第5期11卷 442-455页

作者： Jianxiong Xu Jinxuan wang Hongping Zhang Yidan Chen Xiaojuan Zhang Ying Zhang Ming Xie Jun Xiao Juhui Qiu guixue wang Key Laboratory for Biorheological Science and Technology of Ministry of Education State and Local Joint Engineering Laboratory for Vascular ImplantsBioengineering Modern Life Science Experiment Teaching Center of Bioengineering CollegeChongqing UniversityChongqing 400030China Jinfeng Laboratory Chongqing 401329China School of Basic Medical Sciences Chengdu Medical CollegeChengduSichuan 610500China Chongqing Emergency Medical Center(Chongqing University Central Hospital) Chongqing 400014China

The clearance of apoptotic cell debris,containing professional phagocytosis and non-professional phagocytosis,is essential for maintaining the homeostasis of healthy ***,we discovered that endothelial cells could engulf apoptotic cell debris in atherosclerotic ***-cell RNA sequencing(RNA-seq)has revealed a unique endothelial cell subpopulation in atherosclerosis,which was strongly associated with vascular injury-related ***,integrated analysis of three vascular injury-related RNA-seq datasets showed that the expression of scavenger receptor class B type 1(SR-B1)was up-regulated and specifically enriched in the phagocytosis pathway under vascular injury ***-cell RNA-seq and bulk RNA-seq indicate that SR-B1 was highly expressed in a unique endothelial cell subpopulation of mouse aorta and strongly associated with the reorganization of cellular adherent junctions and cytoskeleton which were necessary for ***,SR-B1 was strongly required for endothelial cells to engulf apoptotic cell debris in atherosclerotic plaque of both mouse and human ***,this study demonstrated that apoptotic cell debris could be engulfed by endothelial cells through SR-B1 and associated with the reorganization of cellular adherent junctions and cytoskeleton.

关键词： Apoptotic cell debris Atherosclerosis Endothelial engulfment Single-cell RNA sequencing Scavenger receptor class B type 1

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血管生物力学与力学生物学研究进展

医用生物力学 2024年第1期39卷 17-23页

作者：张红萍赵川榕王贵学重庆大学生物工程学院生物流变科学与技术教育部重点实验室血管植入物开发国家地方联合工程实验室重庆400045 金凤实验室重庆401329

血管生物力学主要探讨血管细胞如何感知力学刺激、力学如何影响疾病发生发展以及开发多种数理模型来分析力学因素对疾病的作用。近年来,血管生物力学领域的研究蓬勃发展,各科研团队从不同方向解析血管的力学生物学过程,以期深入了解血... 详细信息

血管生物力学主要探讨血管细胞如何感知力学刺激、力学如何影响疾病发生发展以及开发多种数理模型来分析力学因素对疾病的作用。近年来,血管生物力学领域的研究蓬勃发展,各科研团队从不同方向解析血管的力学生物学过程,以期深入了解血管生物力学因素影响各种血管疾病进程的调控机制,为心脑血管疾病的防治提供基于力学生物学的理论基础。本文基于国内外专家团队并结合本团队研究工作,对血管力学生物学领域近期的研究热点和新兴趋势进行总结探讨,为把握血管力学生物学领域热点和探索新的研究方向提供系统框架。

关键词：血管生物力学力学生物学计算流体动力学血管支架动脉瘤有限元分析

High performance of multi-layered alternating Ni-Fe-P and Co-P films for hydrogen evolution

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Green Energy & Environment 2022年第1期7卷 75-85页

作者： Zunhang Lv Kaihang wang Yingying Si Zihan Li Tianpeng Yu Xin Liu guixue wang Guangwen Xie Luhua Jiang College of Materials Science and Engineering Qingdao University of Science and TechnologyQingdao266042China

Judiciously engineering the electrocatalysts is attractive and challenging to exploit materials with high electrocatalytic performance for hydrogen evolution ***,we successfully perform the interface engineering by alternately depositing Co±P and Ni±Fe±P films on nickel foam,via facile electroless plating and de-alloying *** work shows that there is a significant effect of de-alloying process on alloy *** electronic structure of layered alloys is improved by interface *** multilayer strategy significantly promotes the charge ***,the Co±P/Ni±Fe±P/NF electrode fabricated by interface engineering exhibits excellent electrocatalytic hydrogen evolution activity with an overpotential of 43.4 mV at 10 mA cm^(-2)and long-term durability for 72 h in alkaline medium(1 mol L^(-1)KOH).The innovative strategy of this work may aid further development of commercial electrocatalysts.

关键词： Interface engineering Electroless plating Amorphous materials Hydrogen evolution reaction Electrocatalysts

The recent advances and future perspectives of genetic compensation studies in the zebrafish model

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Genes & Diseases 2023年第2期10卷 468-479页

作者： Muhammad Abdul Rouf Lin Wen Yoga Mahendra Jinxuan wang Kun Zhang Shuang Liang Yuming wang Zhenggong Li Yeqi wang guixue wang Key Laboratory for Biorheological Science and Technology of Ministry of Education State and Local Joint Engineering Laboratory for Vascular Implants Bioengineering College of Chongqing University Chongqing 400030China Center of Cardiology Chongqing General HospitalUniversity of Chinese Academy of SciencesChongqing 400013China

Genetic compensation is a remarkable biological concept to explain the genetic robustness in an organism to maintain its fitness and viability if there is a disruption occurred in the genetic variation by ***,the underlying mechanism in genetic compensation remain *** initial concept of genetic compensation has been studied in model organisms when there was a discrepancy between knockout-mediated and knockdown-mediated *** the zebrafish model,several studies have reported that zebrafish mutants did not exhibit severe phenotype as shown in zebrafish morphants for the same *** phenomenon in zebrafish mutants but not morphants is due to the response of genetic *** 2019,two amazing works partially uncovered genetic compensation could be triggered by the upregulation of compensating genes through regulating NMD and/or PTC-bearing mRNA in collaboration with epigenetic machinery in mutant *** this review,we would like to update the recent advances and future perspectives of genetic compensation studies,which including the hypothesis of time-dependent involvement and addressing the discrepancy between knockout-mediated and knockdown-mediated to study gene function in the zebrafish *** last,the study of genetic compensation could be a potential therapeutic strategy to treat human genetic disorder related diseases.

关键词： Epigenetic machinery Genetic compensation Genetic robustness Knockdown-mediated Knockout-mediated NMD PTC Zebrafish

Particle Size-Controlled Oxygen Reduction and Evolution Reaction Nanocatalysts Regulate Ru(bpy)_(3)^(2+)’s Dual-potential Electrochemiluminescence for Sandwich Immunoassay

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Research 2023年第4期2023卷 701-715页

作者： Shijun wang Shu Zhu Ziqi Kang Xiangxiu wang Zixin Deng Kun Hu Jianjun Hu Xiancheng Liu guixue wang Guangchao Zang Yuchan Zhang Institute of Life Science and Laboratory of Tissue and Cell Biology Lab Teaching&Management CenterChongqing Medical UniversityChongqing 400016China Key Laboratory for Biorheological Science and Technology of Ministry of Education State and Local Joint Engineering Laboratory for Vascular ImplantsBioengineering College of Chongqing UniversityChongqing 400030China Jinfeng Laboratory Chongqing 401329China Department of Pathophysiology Chongqing Medical UniversityChongqing 400016China Department of Pathology Guizhou Provincial People’s HospitalGuiyangGuizhou 550002China

Multiple signal strategies remarkably improve the accuracy and efficiency of electrochemiluminescence(ECL)immunoassays,but the lack of potential-resolved luminophore pairs and chemical cross talk hinders their *** this study,we synthesized a series of gold nanoparticles(AuNPs)/reduced graphene oxide(Au/rGO)composites as adjustable oxygen reduction reaction and oxygen evolution reaction catalysts to promote and modulate tris(2,2′-bipyridine)ruthenium(II)(Ru(bpy)_(3)^(2+))’s multisignal *** the increase in the diameter of AuNPs(3 to 30 nm),their ability to promote Ru(bpy)_(3)^(2+)’s anodic ECL was first impaired and then strengthened,and cathodic ECL was first enhanced and then ***/rGOs with medium-small and medium-large AuNP diameters remarkably increased Ru(bpy)_(3)^(2+)’s cathodic and anodic luminescence,***,the stimulation effects of Au/rGOs were superior to those of most existing Ru(bpy)_(3)^(2+)***,we proposed a novel ratiometric immunosensor construction strategy using Ru(bpy)_(3)^(2+)’s luminescence promoter rather than luminophores as tags of antibodies to achieve signal *** method avoids signal cross talk between luminophores and their respective co-reactants,which achieved a good linear range of 10−7 to 10−1 ng/ml and a limit of detection of 0.33 fg/ml for detecting carcinoembryonic *** study addresses the previous scarcity of the macromolecular co-reactants of Ru(bpy)_(3)^(2+),broadening its application in biomaterial ***,the systematic clarification of the detailed mechanisms for converting the potential-resolved luminescence of Ru(bpy)_(3)^(2+)could facilitate an in-depth understanding of the ECL process and should inspire new designs of Ru(bpy)_(3)^(2+)luminescence enhancers or applications of Au/rGOs to other *** work removes some impediments to the development of multisignal ECL biodetection systems and provides vitality into their

关键词： luminescence potential reactant

Cathepsin K contributed to disturbed flow-induced atherosclerosis is dependent on integrin-actin cytoskeleton–NF–κB pathway

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Genes & Diseases 2023年第2期10卷 583-595页

作者： Fei Fang Tang Feng Jianwei Li Huaiyi Zhang Qin wang Yidan Chen guixue wang Yang Shen Xiaoheng Liu Institute of Biomedical Engineering West China School of Basic Medical Sciences & Forensic MedicineSichuan UniversityChengduSichuan 610041China Department of Endocrinology and Metabolism West China HospitalSichuan UniversityChengduSichuan 610041China Key Laboratory for Biorheological Science and Technology of Ministry of Education State and Local Joint Engineering Laboratory for Vascular ImplantsCollege of BioengineeringChongqing UniversityChongqing 400030China

Atherosclerosis is a chronic inflammatory disease, occurring preferentially in bifurcation, branching, and bending of blood vessels exposed to disturbed flow. Disturbed flow in atheroprone areas activates elevated proteases, degrading elastin lamellae and collagenous matrix, resulting in endothelial dysfunction and vascular remodeling. As a mediator for extracellular matrix protein degradation, cathepsin K (CTSK) was directly regulated by hemodynamics and contributed to atherosclerosis. The mechanism of CTSK responding to disturbed flow and contributing to disturbed flow-induced atherosclerosis is unclear. In this study, the partial carotid ligation model of mice and in vitro disturbed shear stress model were constructed to explore the contribution and potential mechanism of CTSK in atherosclerosis. Our results indicated that CTSK elevated in the disturbed flow area in vivo and in vitro along with endothelial inflammation and atherogenesis. Additionally, the expression of integrin αvβ3 was upregulated in these atheroprone areas. We found that inhibition of the integrin αvβ3-cytoskeleton pathway could significantly block the activation of NF-κB and the expression of CTSK. Collectively, our findings unraveled that disturbed flow induces increased CTSK expression, and contributes to endothelial inflammation and vascular remodeling, leading to atherogenesis eventually. This study is helpful to provide new enlightenment for the therapy of atherosclerosis.

关键词： Atherosclerosis Cathepsin K Disturbed flow Integrin NF-κB