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Two Inhibitors Against the 3C-Like Proteases of Swine Coronavirus and Feline Coronavirus

Virologica Sinica 2021年第6期36卷 1421-1430页

作者： Mengxin Zhou Yutong Han Mengxia Li Gang Ye guiqing peng State Key Laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural UniversityWuhan430070China Key Laboratory of Preventive Veterinary Medicine in Hubei Province The Cooperative Innovation Center for Sustainable Pig ProductionWuhan430070China

Coronaviruses(CoVs)are important human and animal pathogens that cause respiratory and gastrointestinal *** epidemic diarrhoea(PED),characterized by severe diarrhoea and vomiting in pigs,is a highly lethal disease caused by porcine epidemic diarrhoea virus(PEDV)and causes substantial losses in the swine industry ***,currently available commercial drugs have not shown great therapeutic *** this study,a fluorescence resonance energy transfer(FRET)-based assay was applied to screen a library containing 1,590 compounds and identified two compounds,3-(aminocarbonyl)-1-phenylpyridinium and 2,3-dichloronaphthoquinone,that target the 3C-like protease(3CL^(pro))of *** compounds are of low molecular weight(MW)and greatly inhibited the activity of this enzyme(IC_(50) values were obtained in this study).Furthermore,these compounds exhibited antiviral capacity against another member of the CoV family,feline infectious peritonitis virus(FIPV).Here,the inhibitory effects of these compounds against CoVs on Vero cells and feline kidney cells were identified(with EC_(50) values)and cell viability assays were *** results of putative molecular docking models indicate that these compounds,labeled compound 1 and compound 2,contact the conserved active sites(Cys144,Glu165,Gln191)of 3CL^(pro) via hydrogen *** findings provide insight into the antiviral activities of compounds 1 and 2 that may facilitate future research on anti-CoV drugs.

关键词： Coronavirus(CoVs) Inhibitor 3C-like Protease Porcine epidemic diarrhoea virus(PEDV) Feline infectious peritonitis virus(FIPV)

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Nano-bubble hydrogen water:An effective therapeutic agent against inflammation related disease caused by viral infection in zebrafish model

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Virologica Sinica 2022年第2期37卷 277-283页

作者： Chen Li Yiran Cao Fukuda Kohei Haihong Hao guiqing peng Can Cheng Jing Ye State Key Laboratory of Agricultural Microbiology College of FisheriesHuazhong Agricultural UniversityWuhan430070China Wuhan Britain-China School Wuhan430070China Shenzhen Nano Buddy Technology Co. Ltd.Shenzhen518000China MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products Huazhong Agricultural UniversityWuhan430070China State Key Laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural UniversityWuhan430070China

Since the anti-inflammatory effect of hydrogen has been widely known,it was supposed that hydrogen could suppress tissue damage by inhibiting virus-related inflammatory ***,hydrogen is slightly soluble in water,which leads to poor effect of oral hydrogen-rich water *** this study,the nano-bubble hydrogen water(nano-HW)(about 0.7 ppm)was prepared and its therapeutic effect against viral infection was investigated by utilizing spring viraemia of carp virus(SVCV)-infected zebrafish as ***-month-old zebrafish were divided into nano-HW treatment-treated group and aquaculture water treated group(control group).The results revealed that the cumulative mortality rate of SVCV-infected zebrafish was reduced by 40%after treatment with nano-bubble hydrogen water,and q RT-PCR results showed that SVCV replication was significantly *** examination staining showed that SVCV infection caused tissue damage was greatly alleviated after treatment with nano-bubble hydrogen ***,SVCV infection caused reactive oxygen species(ROS)accumulation was significantly reduced upon nano-HW *** level of proinflammatory cytokines IL-1β,IL-8,and TNF-αwas remarkably reduced in the nano-HW-treated group in vivo and in *** together,our data demonstrated for the first time that nano-HW could inhibit the inflammatory response caused by viral infection in zebrafish,which suggests that nano-HW can be applied to antiviral research,and provides a novel therapeutic strategy for virus-caused inflammation related disease.

关键词： Nano-bubble hydrogen water(nano-HW) Spring viraemia of carp virus(SVCV) Zebrafish Inflammation

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Comparison of lentiviruses pseudotyped with S proteins from coronaviruses and cell tropisms of porcine coronaviruses

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Virologica Sinica 2016年第1期31卷 49-56页

作者： Jingjing Wang Feng Deng Gang Ye Wanyu Dong Anjun Zheng Qigai He guiqing peng State Key Laboratory of Agricultural Microbiology Huazhong Agricultural University College of Veterinary Medicine Huazhong Agricultural University The Cooperative Innovation Center for Sustainable Pig Production Huazhong Agricultural University

The surface glycoproteins of coronaviruses play an important role in receptor binding and cell entry. Different coronaviruses interact with their specific receptors to enter host cells. Lentiviruses pseudotyped with their spike proteins(S) were compared to analyze the entry efficiency of various coronaviruses. Our results indicated that S proteins from different coronaviruses displayed varied abilities to mediate pseudotyped virus infection. Furthermore, the cell tropisms of porcine epidemic diarrhea virus(PEDV) and transmissible gastroenteritis virus(TGEV) have been characterized by live and pseudotyped viruses. Both live and pseudoviruses could infected VeroCCL-81(monkey kidney), Huh-7(human liver), and PK-15(pig kidney) cells efficiently. CCL94(cat kidney) cells could be infected efficiently by TGEV but not PEDV. Overall, our study provides new insights into the mechanisms of viral entry and forms a basis for antiviral drug screening.

关键词： Coronavirus spike proteins receptor binding cell entry pseudotyped virus

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A Dimerization-Dependent Mechanism Drives the Functions of PRRSV nsp11 as Endoribonuclease and Beta Interferon Antagonist

A Dimerization-Dependent Mechanism Drives the Functions of P...

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第十届全国免疫学学术大会

作者： Yuejun Shi Shaobo Xiao guiqing peng State Key Laboratory of Agricultural Microbiology Huazhong Agricultural University College of Veterinary Medicine Huazhong Agricultural University

The porcine reproductive and respiratory syndrome virus(PRRSV)RNA endoribonuclease nsp11 belongs to the Xendo U superfamily,and plays a crucial role in viral RNA *** date,no structure has been reported for an arterivirus endoribonuclease ***,we report the first crystal structure of the arterivirus nsp11 from PRRSV,which exhibits a unique structure and assembles into an asymmetric dimer that is completely different from the hexameric structure of coronavirus ***,the structures of the PRRSV nsp11 and coronavirus nsp15 cocatalytic domains were perfectly superimposed,especially in the"active site loop"and"supporting loop"***,our biochemical data demonstrated that PRRSV nsp11 was dimeric form in *** of the major dimerization site determinants(Ser74 and Phe76)in the dimerization interface destabilized the stable dimers in solution and severely diminished the endoribonuclease activity,indicating that the dimer was the biologically functional *** the dimeric structure,the catalytic loop(His129-His144)and supporting loop(Val162-Thr179)are packed against one another and stabilized by monomer-monomer *** results support the hypothesis that these two loops may be flexibly disordered when the dimeric forms are ***,the mutagenesis analysis demonstrated that the endoribonuclease activity was essential for nsp11s function as an Interferon-β*** findings may help elucidate the mechanism underlying PRRSV replication and may provide great potential for antiviral drug development.

关键词： arterivirus PRRSV endoribonuclease nsp11 structure Beta Interferon Antagonist

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Occurrence Regularity of Tobacco Cutworm Moth (Spodoptera Litura) in Low-Altitude Tobacco Cultivation Areas in Lincang

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Plant Diseases and Pests 2012年第2期3卷 42-45,48页

作者： Mingwen YANG Xuebing ZHANG Ping YA guiqing peng Ping CHEN Jianjun YU Dongcheng ZHANG Lincang Agricultural Extension Station Lincang Branch of Yunnan Tobacco Company Zhenkang Branch of Lincang Tobacco Company

[ Objective ] The paper was to study the occurrence and damage regularity of tobacco cutworm moth （Spodoptera Litura Fabricius） in low-altitude area in Lincang city. [ Method] The adult sex attractant monitoring, field investigation of occurrence and damage, and observation of continuous generation development of natural populations were carried out in natural flue-cured tobacco fields. [ Result] In field growth period of flue-cured tobacco under low-altitude of 1 000 - 1300 m, tobacco cutworm moth occurred for 4 generations, the duration of each generation was about 33 d, and the main injurious generation was the third generation. [ Conclusion] The paper provided reference for comprehensive control of tobacco cutworm moth in the low altitude areas.

关键词： Low-altitude tobacco cultivation areas Tobacco cutworm moth Occurrence regularity China

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“两家”融合“四循环”一体培养牧医领军人才的实践

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学位与研究生教育 2023年第4期 20-26页

作者：侯顺祁婧彭贵青赵书红华中农业大学马克思主义学院武汉430070 华中农业大学研究生院培养处武汉430070 华中农业大学动物科学技术学院、动物医学院武汉430070

在当前国家战略和行业发展迫切需求高层次创新型人才的背景下,研究生培养模式的改革亟待进行。华中农业大学针对牧医行业产教融合之困和领军人才培养之难,形成“科学家+企业家”的“两家”融合育人理念,构建“产业‒产业”问题循环、“... 详细信息

在当前国家战略和行业发展迫切需求高层次创新型人才的背景下,研究生培养模式的改革亟待进行。华中农业大学针对牧医行业产教融合之困和领军人才培养之难,形成“科学家+企业家”的“两家”融合育人理念,构建“产业‒产业”问题循环、“科研创新平台‒行业龙头企业”平台循环、“论文‒产品‒商品”成果循环、“科研创新者‒行业引领者”身份循环的“四循环”人才培养模式,以期为牧医行业发展提供人才支撑。

关键词：科学家+企业家 “四循环”模式牧医领军人才研究生教育

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射干麻黄汤联合西药治疗支气管哮喘随机平行对照研究

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实用中医内科杂志 2015年第12期29卷 114-116页

作者：彭贵清苏州市中医医院呼吸科江苏苏州215009

[目的]观察射干麻黄汤联合西药治疗支气管哮喘疗效。[方法]使用随机平行对照方法,将120例门诊患者按就诊顺序号法简单随机分为两组;禁食生冷、刺激、油炸及海鲜等食品。对照组60例信必可,1吸/次,2次/d;舒利迭,1吸/次,2次/d。治疗组60例... 详细信息

[目的]观察射干麻黄汤联合西药治疗支气管哮喘疗效。[方法]使用随机平行对照方法,将120例门诊患者按就诊顺序号法简单随机分为两组;禁食生冷、刺激、油炸及海鲜等食品。对照组60例信必可,1吸/次,2次/d;舒利迭,1吸/次,2次/d。治疗组60例射干麻黄汤(麻黄、五味子、蝉蜕各5g,全蝎、细辛各3g,僵蚕、射干、款冬花、半夏各10g,甘草6g;咳痰稀薄且泡沫居多、舌苔白滑、脉弦紧加桂枝、白芥子、干姜;喉中痰鸣音较大、喘气粗、痰色黄加苏子、桑白皮、瓜蒌、黄芩;鼻塞、喷嚏、流涕、咽、耳、鼻发痒加防风、荆芥、辛夷),1剂/d,水煎400m L,早晚口服,200m L/次;西药治疗同对照组。连续治疗15d为1疗程。观测临床症状、心率指标、呼吸指标、不良反应。治疗1疗程,判定疗效。[结果]治疗组显效39例,有效19例,无效2例,总有效率96.67%。对照组显效26例,有效12例,无效22例,总有效率63.33%。治疗组疗效优于对照组(P<0.01)。心率、呼吸指标两组均有改善(P<0.01),治疗组改善优于对照组(P<0.01)。[结论]射干麻黄汤联合西药治疗支气管哮喘疗效满意,无严重不良反应,值得推广。

关键词：支气管哮喘射干麻黄汤信必可舒利迭心率指标呼吸指标中西医结合治疗随机平行对照研究

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Crystal structures of Babesia microti Lactate Dehydrogenase BmLDH reveal a critical role for Arg99 in catalysis

Crystal structures of Babesia microti Lactate Dehydrogenase ...

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中国畜牧兽医学会兽医寄生虫学分会第一届青年科学家学术论...

作者： Long Yu Zhou Shen Qin Liu Xueyan Zhan Xiaoyin Luo Xiaomeng An Yali Sun Muxiac Li Sen Wang Zheng Nie Yangsiqi Ao Yangnan Zhao guiqing peng Choukri Ben Mamoun Lan He Junlong Zhao State Key Laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural University Department of Internal Medicine Section of Infectious DiseasesYale School of Medicine

The tick and transfusion-transmitted human pathogen Babesia microti infects host erythrocytes to cause the pathological symptoms associated with human babesiosis,an emerging disease with worldwide distribution and potentially fatal clinical *** currently recommended for the treatment of babesiosis are associated with a high failure rate and significant adverse events,highlighting the urgent need for more effective and safer babesiosis *** other apicomplexan parasites,*** lacks a canonical lactate dehydrogenase but instead expresses a unique enzyme,BmLDH,acquired through evolution by horizontal transfer from a mammalian *** we report the crystal structures of BmLDH in apo state and ternary complex (enzyme-NADH-oxamate) solved at 2.79 and 1.89 *** of these structures revealed that upon binding to the coenzyme and substrate,the active pocket of BmLDH undergoes a major conformational change from an opened and disordered to a closed and stabilized *** assays using wild type and mutant *** and human LDHs identified Arg99 as a critical residue for the catalytic activity of BmLDH but not its human ***,mutation of Arg99 to Ala had no impact on the overall structure and affinity of BmLDH to NADH,but dramatically altered the closure of the enzyme’s active *** these structural and biochemical data highlight significant differences between *** and human LDH enzymes and suggest that BmLDH could be a suitable target for the development of selective anti-babesial inhibitors.

关键词： lactate dehydrogenase crystal structure growth inhibitor catalytic mechanism babesiosis

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猫传染性腹膜炎诊断研究进展

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中国动物传染病学报 2022年第2期30卷 208-215页

作者：刘一楠陈奕熹汪刚毛军福彭贵青华中农业大学动物科技学院武汉430070

猫传染性腹膜炎(FIP)是由猫传染性腹膜炎病毒(FIPV)引起的全身性、致死性疾病。FIPV属于猫冠状病毒(FCoV),主要感染单核细胞,具有很强的致病性。另一种非致病性冠状病毒,称为猫肠道冠状病毒(FECV),存在于大多数健康猫肠道内。根据血清... 详细信息

猫传染性腹膜炎(FIP)是由猫传染性腹膜炎病毒(FIPV)引起的全身性、致死性疾病。FIPV属于猫冠状病毒(FCoV),主要感染单核细胞,具有很强的致病性。另一种非致病性冠状病毒,称为猫肠道冠状病毒(FECV),存在于大多数健康猫肠道内。根据血清学和基因序列,可以将FCoV分为Ⅰ型和Ⅱ型,FECV和FIPV在基因组上有一定差异,FECV通常不致死,只引起猫轻微的肠道症状,而FIPV能引起猫的致死性腹膜炎。本文对现有的FIP的诊断方法进行了系统阐述,为FIP的临床和实验室诊断提供参考。

关键词：猫传染性腹膜炎病毒猫肠道冠状病毒诊断血清型

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参蛤益肺胶囊对COPD大鼠氧化相关指标的影响

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陕西中医 2010年第8期31卷 1068-1069页

作者：彭贵清孙钢何焕荣江苏省苏州市中医院苏州215003

目的:观察参蛤益肺胶囊对慢性阻塞性肺疾病(COPD)大鼠氧化/抗氧化相关指标的影响。方法:SD大鼠84只,采用烟熏加气道内注入脂多糖(LPS)法复制COPD模型,并予参蛤益肺胶囊治疗,观察治疗后肺系数,血清中超氧化物歧化酶(SOD)活性及丙二醛(MDA... 详细信息

目的:观察参蛤益肺胶囊对慢性阻塞性肺疾病(COPD)大鼠氧化/抗氧化相关指标的影响。方法:SD大鼠84只,采用烟熏加气道内注入脂多糖(LPS)法复制COPD模型,并予参蛤益肺胶囊治疗,观察治疗后肺系数,血清中超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量,肺组织匀浆中谷胱酐肽(GSH)的水平,并测量细支气管内径水平。结果:参蛤组大鼠肺系数较模型组降低(P<0.05),细支气管阻塞程度减轻(P<0.01),血清中SOD活性及肺组织匀浆中GSH含量较模型组增加,血清中MDA含量较模型组降低(P<0.05)。结论:参蛤益肺胶囊治疗COPD与其有一定的抗氧化作用有关。

关键词： @参蛤益肺胶囊/药理学大鼠实验研究

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