检索结果-南通市图书馆

Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment

在线全文

学校读者我要写书评

暂无评论

aBIOTECH 2023年第3期4卷 202-212页

作者： Linli Huang Guangqi Gao Congcong Jiang guimei guo Qiang He Yingjie Zong Chenghong Liu Ping Yang Biotech Research Institute Shanghai Academy of Agricultural Sciences/Shanghai Key Laboratory of Agricultural Genetics and BreedingShanghai 201106China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China

Induced mutations are important for genetic research and *** induced by physical or chemical mutagenesis are usually heterozygous during the early ***,mutations must be fixed prior to phenotyping or field trials,which requires additional rounds of *** culture is an effective method to produce double-haploid(DH)plants that are fixed *** this study,we conducted ethyl methanesulfonate(EMS)-induced mutagenesis of microspore cultures of barley(Hordeum vulgare)cultivar‘Hua30’and landrace‘HTX’.The EMS concentrations were negatively correlated with the efficiency of callus induction and the frequency of mutant plant *** two genotypes showed different regeneration *** phenotypic variation of the regenerated M1 plants and the presence of genome-wide nucleotide mutations,revealed by whole-genome sequencing,highlight the utility of EMS-induced mutagenesis of isolated microspore cultures for developing DH ***-wide analysis of the mutation frequency in the regenerated plants revealed that a considerable proportion of mutations resulted from microspore culture(somaclonal variation)rather than EMS-induced *** addition to producing a population of 1972 homozygous mutant lines that are available for future field trials,this study lays the foundation for optimizing the regeneration efficiency of DH plants and the richness of mutations(mainly by fine-tuning the mutagen dosage).

关键词： Barley Mutagenesis Microspore culture Double-haploid(DH) Homozygous mutant

Genome-Wide Identification of Candidate Genes Associated with β-glucan Traits in a Hulled and Hulless Barley (Hordeum vulgare L.) Population

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2020年第4期89卷 987-997页

作者： Longhua Zhou Ting He guimei guo Jing Li Ruiju Lu Yingbo Li Chenghong Liu Shanghai Academy of Agricultural Sciences Shanghai201106China Shanghai Key Laboratory of Agricultural Genetics and Breeding Shanghai201106China

Barley grain is a valuable source ofβ-glucan,which is an important component of dietary fiber with significant human health *** the genetic basis ofβ-glucan biosynthesis has been widely studied,a genome-wide association study(GWAS)is still required for a scan of the candidate genes related to the complex quantitative trait based on the high-quality barley reference *** this study,a GWAS was conducted using a population composed of 87 barley landraces(39 hulled and 48 hulless,β-glucan from 2.07%to 6.56%)with 191,098 nucleotide polymorphisms(SNPs)markers to cover the chromosomes with the highest *** population was divided into four sub-populations(POP1~POP4),and theβ-glucan content in POP2 was significantly higher than that in other groups,in which most of the hulless barley landraces are from Qinghai-Tibet Plateau in *** seven SNP markers identified by GWAS,two(SNP2 and SNP3)of them showed positive correlation toβ-glucan trait and the remaining five(SNP1,SNP4,SNP5,SNP6 and SNP7)showed the negative *** candidate genes linked to SNP7,HORVU7Hr1G000320 and HORVU7Hr1G000040,belong to the nucleotide triphosphate hydrolase super-family which is probable to affect the activities ofβ-glucan *** candidate gene associated with SNP1,HORVU1Hr1G000010,is possibly involved in sugar *** conclusion,our results provide new insights into the genetic basis ofβ-glucan accumulation in barley grains,and the discovery of new SNP markers distributed in each chromosome and the associated candidate genes will be valuable for the breeding of functional barley varieties with high β-glucan.

关键词： GWAS β-glucan SNP barley grains

Transcriptome Profiling of Barley Cultivar Hua 30 MDEC in Response to Agrobacterium Infection

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2022年第6期91卷 1153-1164页

作者： Yingbo Li Ting He guimei guo Hongwei Xu Yingjie Zong Shuwei Zhang Ruiju Lu Longhua Zhou Chenghong Liu Biotech Research Institute Shanghai Academy of Agricultural SciencesShanghai201403China Shanghai Key Laboratory of Agricultural Genetics and Breeding Shanghai201106China

Agrobacterium-mediated transformation has been widely used in ***,the mechanism in plant cells’response to Agrobacterium infection was very *** mechanism of the determinants in host cell remains obscure,especially in barley,which is recalcitrant for Agrobacterium-mediated *** the present study,microspore-derived embryogenic calli(MDEC)from barley elite cultivar were employed as unique subjects to characterize the mechanisms during the Agrobacterium infection *** 30 MDEC can be successfully infected by ***-sequencing at different infection points(0,2,6,12,24 hpi)was *** average expressional intensity of the whole genomics increased from 0 to 2 hpi,and then decreased *** upregulated than downregulated differentially expressed genes(DEGs)were counted at the same *** enrichment analysis showed that protein modification was significantly overrepresented in upregulated ***-related biological processes,gene expression and cellular metabolic processes were significantly overrepresented in downregulated *** analysis showed that plant defense responses,phenylpropanoid biosynthesis and biosynthesis of amino acids were significantly enriched across the infection time *** DEGs related to defense responses were *** DEGs were upregulated from 2 to 24 *** speculate that these genes are possibly related to Agrobacterium *** findings will provide deep insights into the molecular events occurring during the process of Agrobacterium-mediated transformation.

关键词： Barley Agrobacterium-mediated transformation microspore-derived embryogenic callus transcriptome analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

摩擦自激系统的分岔控制研究

机械强度 2009年第4期31卷 523-526页

作者：郭桂梅天津商业大学机械工程学院天津300134

研究应用Washout滤波器技术对摩擦系统自激振动的分岔控制。首先确定需要引入的Hopf分岔点,然后按Hopf分岔条件确定滤波器的线性增益和非线性增益系数,将系统的亚临界Hopf分岔变为超临界Hopf分岔,使受控系统产生的自激振动幅值大大降低... 详细信息

研究应用Washout滤波器技术对摩擦系统自激振动的分岔控制。首先确定需要引入的Hopf分岔点,然后按Hopf分岔条件确定滤波器的线性增益和非线性增益系数,将系统的亚临界Hopf分岔变为超临界Hopf分岔,使受控系统产生的自激振动幅值大大降低。理论分析和数值仿真表明Washout滤波器法在分岔控制中的有效性。

关键词：自激振动分岔控制 Washout滤波器摩擦

Phytohormones Accumulation and Distribution in Shoots and Roots of Haploid,Diploid and Tetraploid Barley Seedlings Derived from Microspore Culture

在线全文

学校读者我要写书评

暂无评论

Phyton-International Journal of Experimental Botany 2022年第7期91卷 1419-1428页

作者： Longhua Zhou Ting He Jing Li guimei guo Yingbo Li Hongwei Xu Runhong Gao Linli Huang Yifei Wang Ruiju Lu Zhiwei Chen Chenghong Liu Biotech Research Institute Shanghai Academy of Agricultural SciencesShanghai200000China Biotechnology Research Institute Shanghai Key Laboratory of Agricultural Genetics and BreedingShanghai200000China Shanghai Baixin Biotechnology Company Limited Shanghai200000China

Phytohormones play important roles in plant growth and development,and polyploids are thought to be an important method for plant ***,the relationship between ploidy and phytohormone is still *** this study,barley at three ploidy levels were produced by microspore ***,we further analyzed the phytohormone content in the shoots and roots of the three kinds of barley materials to study the effect of ploidy on phytohormones accumulation and *** results showed that Abscisic acid(ABA),gibberellin(GA),jasmonic acid(JA),auxin(IAA),salicylic acid(SA)and cytokinin(CTK)were successfully determined in shoots and roots using LC-MS(liquid chromatography mass spectrometry).By comparing the shoots of the haploid and diploid plants,it was found that the distribution trend of the six phytohormones was consistent,and another consistent trend was found in the roots of the diploid and tetraploid *** addition,we further analyzed the shoot/root ratio of the different phytohormones to identify the potential differences for haploid,diploid and ***,the relationship between ploidy and phytohormone we provided would provide new insights into understanding the new phenotypes that occur in polyploid species.

关键词： Barley polyploidy microspore culture phytohormone shoot root

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

低温胁迫影响大麦小孢子全能性机制解析

上海农业学报 2024年第1期40卷 7-16页

作者：李文瑞李颖波郭桂梅宗营杰周龙华徐红卫刘成洪上海市农业科学院生物技术研究所上海市农业遗传育种重点实验室上海201106 上海师范大学生命科学学院上海201400

为研究低温对小孢子细胞全能性获得的影响,对低温处理0 d、3 d、7 d的大麦小孢子进行体外培养。结果表明:愈伤产量随着低温处理时间增加而显著增长。转录组分析显示,以低温处理0 d为对照,处理3 d和7 d的差异表达基因分别为5290、7103个... 详细信息

为研究低温对小孢子细胞全能性获得的影响,对低温处理0 d、3 d、7 d的大麦小孢子进行体外培养。结果表明:愈伤产量随着低温处理时间增加而显著增长。转录组分析显示,以低温处理0 d为对照,处理3 d和7 d的差异表达基因分别为5290、7103个。对差异表达基因的GO富集分析显示,在处理3 d和7 d显著富集的生物学过程均为碳水化合物代谢过程、赤霉素分解代谢过程、孢粉素生物合成过程等,细胞组分富集种类均为核小体、类囊体、细胞壁等,分子功能富集种类均为蛋白质异源二聚化活性、DNA结合转录因子活性等。KEGG富集分析显示,低温处理3 d和7 d中的通路富集为光合作用-天线蛋白、植物激素信号转导、乙醛酸和二羧酸代谢等。植物激素信号转导通路在上调富集的通路中最显著,相关的基因可能在低温诱导小孢子全能性中起到重要作用。另外,与植物防御机制相关的热休克蛋白和谷胱甘肽转移酶基因也可能参与小孢子重编程过程。

关键词：大麦小孢子低温胁迫转录组分析

变革型领导行为与创造性:内在动机和创造性工作氛围的中介作用——针对中国企业管理实践的分析

在线全文

学校读者我要写书评

暂无评论

科学学与科学技术管理 2008年第3期29卷 189-196页

作者：郭桂梅段兴民西安交通大学管理学院西安710049

以534名中国企业员工为样本分析结果表明,变革型领导行为与员工创造性正相关:内在动机和创造性工作氛围均对变革型领导行为与员工创造性之间的关系具有部分中介作用,作为中介变量的内在动机和创造性工作氛围之间具有正向的互动作用。由... 详细信息

以534名中国企业员工为样本分析结果表明,变革型领导行为与员工创造性正相关:内在动机和创造性工作氛围均对变革型领导行为与员工创造性之间的关系具有部分中介作用,作为中介变量的内在动机和创造性工作氛围之间具有正向的互动作用。由于增加了一条中介途径,使得分析模型对员工创造性变差的解释量由63.9%增加到68.5%。随后,对研究结果进行了讨论,并指出了研究的局限性。

关键词：变革型领导行为内在动机创造性工作氛围创造性

Innovations in metallurgical and materials processes:advancing liquid theory and honoring ***-jie guo's contributions to physical chemistry in metallurgy

在线全文

学校读者我要写书评

暂无评论

Journal of Iron and Steel Research International 2024年第1期31卷 1-2页

作者： Shuai Liu Jing guo Han-jie guo School of Artificial Intelligence Beijing Technology and Business UniversityBeijing100048China School of Metallurgical and Ecological Engineering University of Science and Technology Beijing(USTB)Beijing100083China

Spanning three decades,***-jie guo's research group has dedicated itself to exploring the fundamental theories and practical applications of physical chemistry within the realm of *** under the assumption that molecul... 详细信息

关键词： chemistry liquid complexes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

药物相关性血管炎新认识

中华儿科杂志 2019年第7期57卷 567-570页

作者：郭桂梅何威逊上海交通大学医学院附属新华医院儿肾科 200092 上海交通大学附属儿童医院肾脏科 200062

包括生物制剂在内的许多药物可以诱发血管炎,药物性血管炎发病机制有中性粒细胞胞浆抗体诱导中性粒细胞活化,中性粒细胞胞外诱捕网产生与降解异常以及外源性蛋白质促使机体产生中和药物的自身抗体等。药物性血管炎临床表现与原发性血管... 详细信息

包括生物制剂在内的许多药物可以诱发血管炎,药物性血管炎发病机制有中性粒细胞胞浆抗体诱导中性粒细胞活化,中性粒细胞胞外诱捕网产生与降解异常以及外源性蛋白质促使机体产生中和药物的自身抗体等。药物性血管炎临床表现与原发性血管炎相似,但症状较轻。尚无统一的诊断标准,治疗上首先停用可疑药物,评估受累器官的严重程度和疾病活动性,参照血管炎治疗方案给予激素、免疫抑制剂治疗,预后相对较好。

关键词：原发性血管炎药物相关性中性粒细胞活化中性粒细胞胞浆抗体治疗方案疾病活动性免疫抑制剂生物制剂