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Isolation and Progeny Transmission of Non-Temperature-Sensitive MS-H Vaccine Strains of Mycoplasma synoviae from Temperature-Sensitive MS-H-Vaccinated Laying Breeder Hens

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Food and Nutrition Sciences 2023年第7期14卷 579-588页

作者： Victor M. Petrone-garcia Raquel López-Arellano Inkar Castellanos-Huerta Saeed El-Ashram Ebtesam Al-Olayan Danielle Graham guillermo Tellez-Isaias Departamento de Ciencias Pecuarias Universidad Nacional Autónoma de México (UNAM) Cuautitlan Izcalli Estado de México México Laboratorio No 5: LEDEFAR Unidad de Investigacion Multidisciplinaria Facultad de Estudios Superi-ores Cuautitlan UNAM Cuautitlan Izcalli Estado de México México Department of Poultry Science University of Arkansas Fayetteville USA Department of Zoology Faculty of Science Kafrelsheikh University Kafr El-Sheikh Egypt Department of Zoology College of Science King Saud University Riyadh Saudi Arabia

This pilot study reports the vertical transmission and reverse thermosensitivity of the MS-H vaccine strain of Mycoplasma synoviae (MS) by RAPD in commercial breeders and their progeny. At two weeks of age, breeders were vaccinated with the ts+ MS-H strain. At 9 weeks of age, an outbreak of infectious synovitis (IS) was detected in the progeny. Tracheal swab samples were collected from breeders at 24, 39, 48, and 70 weeks of age. At 9 weeks, pullets swab from the elbow joints were collected. RAPD was performed on the isolates at 39.5°C, and the same ts- MS-H strains were identified in the breeder hens and their progeny. Tracheal swabs from breeder hens were negative to MS isolation at 37°C and 39.5°C at 24- and 39-weeks. MS isolation was recovered from tracheal swabs from 9/10 and 10/10 breeders at 48- and 70- week. At 9 weeks of age in the progeny, MS was isolated from tracheal swabs of 10/10 from non-IS pullets. MS was isolated from 9/10 joints samples. The isolates from breeder hens and their progeny showed non-significant differences in five antimycoplasmic MIC100 values;otherwise, enrofloxacin presented a significant difference in MIC100 value (p < 0.05). This investigation demonstrated the reversal of the thermosensitivity, pathogenicity, and vertical transmission of the MS-H strain. Consequently, it is crucial to contemplate the danger of reversing pathogenicity and transmission to progeny when applying the MS-H vaccine strain.

关键词： Vertical Transmission Thermosensitivity Mycoplasma synoviae RAPD Vax-Safe®

让肾脏健康人人可享,处处可及——从预防到诊治

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肾脏病与透析肾移植杂志 2020年第1期29卷 1-7页

作者： Philip Kam Tao guillermo garcia garcia Siu Fai Lui Sharon Andreoli Winston Wing Shing Fung Anne Hradsky Latha Kumaraswami Vassilios Liakopoulos Ziyoda Rakhimova Gamal Saadi Luisa Strani Ifeoma Ulasi Kamyar Kalantar Zadeh 无 Department of Medicine and Therapeutics Carol&Richard Yu PD Research CentrePrince of Wales HospitalChinese University of Hong KongHong Kong Nephrology Service Hospital Civil de Guadalajara Fray Antonio AlcaldeUniversity of Guadalajara Health Sciences CenterGuadalajaraJaliscoMexico Division of Health System Policy and ManagementJockey Club School of Public Health and Primary CareThe Chinese University of Hong KongHong Kong James Whitcomb Riley Hospital for Children Indiana University School of MedicineIndianapolisIndianaUSA World Kidney Day Office BrusselsBelgium Tanker Foundation ChennaiIndia Division of Nephrology and Hypertension 1st Department of Internal MedicineAHEPA HospitalAristotle University of ThessalonikiThessalonikiGreece Nephrology Unit Department of Internal MedicineFaculty of MedicineCairo UniversityGizaEgypt Renal Unit Department of MedicineCollege of MedicineUniversity of NigeriaItuku OzallaEnuguNigeria Division of Nephrology and Hypertension and Kidney Transplantation University of California Irvine School of MedicineOrangeCaliforniaUSA the World Kidney Day Steering Committee

Around 850 million people currently are affected by different types of kidney *** to 1 in 10 adults worldwide has chronic kidney disease(CKD),which is invariably irreversible and mostly *** global burden of CKD is increasing,and CKD is projected to become the fifth most common cause of years of life lost globally by *** CKD remains uncontrolled and if the affected person survives the ravages of cardiovascular and other complications of the disease,CKD progresses to endstage kidney disease,where life cannot be sustained without dialysis therapy or kidney ***,CKD is a major cause of catastrophic health expenditure.

关键词： awareness detection kidney diseases prevention

Transcatheter mitral valve repair in nonagenarians

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Journal of Geriatric Cardiology 2022年第1期19卷 90-94页

作者： Pedro Luis Cepas-Guillén Isaac Pascual Eulogio garcia Pilar Jimenez-Quevedo Alfonso Jurado-Roman Tomás Benito-González Rodrigo Estevez-Loureiro Pedro Li Dabit Arzamendi Bruno Melica Eduardo Infante de Oliveira Pedro Martín Lorenzo Felipe Fernández-Vázquez guillermo Galeote Luis Nombela-Franco Leire Unzue Pablo Avanzas Manel Sabate Xavier Freixa Cardiology Department Cardiovascular Institute(ICCV)Hospital ClinicIDIBAPSUniversity of BarcelonaBarcelonaSpain Interventional Cardiology Unit Hospital Universitario Central de AsturiasDepartment of MedicineUniversity of OviedoOviedoSpain Servicio de Hemodinámica y Cardiología Intervencionista HM CIEC-Centro Integral de Enfermedades CardiovascularesHospital Universitario HM MontepríncipeHM HospitalesMadridSpain Facultad de Medicina Universidad CEU San PabloMadridSpain Cardiology Department Cardiovascular InstituteHospital Clínico San CarlosIdISSCMadridSpain Division of Interventional Cardiology University Hospital La PazIdiPAZCIBER-CVMadridSpain Department of Cardiology University Hospital of LeónLeónSpain Interventional Cardiology Unit HospitalÁlvaro CunqueiroVigoSpain Interventional Cardiology Unit Hospital Sant Pau i Santa CreuBarcelonaSpain Serviço de Cardiologia Centro Hospitalar de Vila Nova de Gaia/Espinho-EPEVila Nova de GaiaPortugal Serviço de Cardiologia Hospital de Santa MariaCentro Hospitalar Lisboa NorteCentro Académico Médico de LisboaLisboaPortugal Servicio de Cardiología Hospital Universitario de Gran Canaria Dr.NegrínLas Palmas de Gran CanariaSpain

Given the increase in life expectancy indeveloped countries,nonagenarian population will become clinically and numerically relevant in our daily routine practice in the near *** has been observed to exert a profound influence on the prevalence of severe mitral regurgitation (MR) in the population.[1]Mitral valve surgery remains the gold standard of care for patients with symptomatic severe MR.

关键词： mitral routine surgery

Hypersensitivity to Aspirin as a Factor for Poor Control in Hereditary Angioedema

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Journal of Biosciences and Medicines 2017年第3期5卷 39-54页

作者： Carlos Alberto garcia-Lopez Maria Eugenia Vargas-Camano Emmanuel Alcazar-Casarin guillermo Quintana Mexiac Pablo Leon Gomez Mario Alberto Ynga-Durand Benjamin Lopez-Velazquez Maria Isabel Castrejon-Vazquez Servicio Inmunologia Clinica y Alergia Centro Medico Nacional“20 de Noviembre”ISSSTEMexico CityMexico Laboratorio de Inmunidad de Mucosas Seccion de Investigacion y PosgradoEscuela Superior de MedicinaInstituto Politecnico NacionalMexico CityMexico

Background: Hereditary angioedema (HAE) is a primary immunodeficiency disorder characterized by C1 complement inhibitor deficiency and unregulated activation of complement. Aspirin hypersensitivity is related to an increase in the amount of leukotrienes with eosinophil and mast cell activation and increased levels of glandular kallikrein with upregulated local conversion of bradykinin. Both conditions can be present in the same patient. Objectives: We present five patients with HAE;they were all being treated in similar ways according to the therapeuthic options available in our institute (danazol). However, three of them had recurrent episodes of angioedema;in these cases, it was identified aspirin hypersensitivity as a cause of poor disease control. A review of the literature is included. Case Presentation: We present the cases of four females and one male (age range 21 - 58 years) with type I HAE. Subjects were all ISSSTE beneficiaries (state workers) treated at the National Medical Center “20 de Noviembre”. Aspirin hypersensitivity was identified in three patients. Elimination of NSAIDs along with dietary elimination of high salicylate-containing foods improved control of angioedema crisis (severe and/ or recurrent episodes). Discussion: Aspirin hypersensitivity was identified as a factor for poor control in our patients with HAE. Such cases improved with dietary elimination of high salicylate-containing foods and avoidance of NSAIDs. Conclusions: This is the first report of patients with HAE and aspirin hypersensitivity as a cause of poor control. We recommend a deliberate search of these comorbidities, especially in cases of poor disease control. Further studies are needed to continue the investigation on this topic.

关键词： Hereditary Angioedema Immunodeficiency Complement Salicylates

Effect of Mushroom Extract Hericium erinaceus on Spatial Memory and Morphology of Neurons in the CA1 and CA3 Regions of the Hippocampus in Ovariectomized Rats

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Open Journal of Veterinary Medicine 2024年第7期14卷 163-179页

作者： Lucia garcia-Delgado Mariana Monserrath Verdía-Venegas Jacinto Bañuelos-Pineda Esther Albarrán-Rodríguez guillermo Nolasco-Rodriguez José Ricardo Cuellar-Pérez Sergio Fausto-Guerra Manuel Rosales-Cortés Master in Behavioral Sciences Institute of Neurosciences University of Guadalajara Zapopan Mexico Department of Veterinary Medicine Division of Veterinary Sciences CUCBA University of Guadalajara Zapopan Mexico Botany and Zoology Laboratory CUCBA University of Guadalajara Zapopan Mexico

Various studies indicate that low levels of estradiol negatively impact cognitive abilities. Extracts from the fungus Hericium erinaceus (HE) contain bioactive components that promote the proper functioning of the nervous system and potential effects on protection against neurodegenerative diseases, including dementia and motor dysfunctions. The objective was to evaluate the effects of the administration of the HE mushroom extract on visuospatial memory and morphology of neurons in the CA1 and CA3 regions of the hippocampus in ovariectomized rats. 40 young Wistar rats weighing 90 ± 10 g BW were used, which were distributed into four groups of 10 animals;Control Group, non-ovariectomized and untreated rats;Group E2, ovariectomized rats treated with estradiol (2 μg/kg/body weight);Group HE, ovariectomized rats treated with the extract of the fungus Hericium erinaceus (0.5 mg/kg body weight) and Group Ovx/ST, ovariectomized rats, without treatment. The animals were tested in the Barnes and Open Field maze, then they were sacrificed, and their brains were obtained to perform a histological analysis of neuronal morphology in the CA1 and CA3 areas of the hippocampus. The most outstanding results showed that the Ovx/ST group recorded the longest time to arrive at the escape box and stay in the Barnes maze. A correlation was observed between neuronal damage and function;in the groups that did not present satisfactory performance in the maze tests, morphological alterations were identified such as the presence of some neuronal somata with degeneration characteristics such as pyknosis, nuclear basophilia and shrinkage of the cells. Its soma, as well as a decrease in the nuclear area of CA1 and CA3 neurons. It is concluded that the fungus Hericium erinaceus exerted a neuroprotective effect on the neuronal bodies of the hippocampus, associated with better performance in the visuospatial recognition memory test.

关键词： Ovariectomized Rats Ovarian Hypofunction Estradiol Hericium erinaceus Barnes Circular Labyrinth Open Field Labyrinth

Genetic variability of Appaloosa horses:a study of a closed breeding population from Argentina

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Frontiers of Agricultural Science and Engineering 2014年第3期1卷 175-178页

作者： Claudia Malena CORBI-BOTTO Sebastian Andres SADABA Elina Ines FRANCISCO Paula Belen KALEMKERIAN Juan Pedro LIRON Egle Etel VILLEGAS-CASTAGNASSO guillermo GIOVAMBATTISTA Pilar PERAL-garcia Silvina DIAZ Instituto de Genética Veterinaria“Ing.Fernando N Dulout”(IGEVET)-CCT La Plata-CONICET Facultad de Ciencias VeterinariasUniversidad Nacional de La PlataLa PlataArgentina Research Fellow from Consejo Nacional de Invetigaciones Cientificas y Tecnicas(CONICET) Argentina Research Fellow from Universidad Nacional de la Plata(UNLP) Argentina

The genetic diversity and structure of 72 Appaloosa horses belonging to a closed breeding population from an ecological reserve in Buenos Aires,Argentina,was investigated using eight microsatellite markers from the International Society for Animal Genetics *** data showed that this Appaloosa horse population had an elevated degree of genetic diversity(He=0.746)and did not present a significant increase of homozygous individuals(F_(IS-0)).However,the short tandem repeats,AHT5,ASB2,HTG10 and VHL20,were not in Hardy-Weinberg equilibrium(P-value<0.05).Genetic relationships between this population and other well known horse breeds showed that Appaloosa horses from Argentina could have had their origin in the horses of the Nez Perce’s people in Idaho while other Appaloosa horses may have had influences from Andalusian and Lusitano *** closed breeding population conserves an important degree of Appaloosa genetic diversity and notwithstanding its particular breeding characteristics,represents a valuable genetic resource for conservation.

关键词： horse genetic diversity microsatellite Appaloosa population structure conservation

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2018世界肾脏日：关注女性和肾脏疾病

肾脏病与透析肾移植杂志 2018年第1期27卷 1-6页

作者： Giorgina B.Piccoli Mona Alrukhaimi Zhi-Hong Liu Elena Zakharova Adeera Levin Philip Kam Tao Li guillermo garcia-garcia Mohammed Benghanem-Gharbi Kamyar Kalantar-Zadeh Charles Kernahan Latha Kumaraswami Giorgina Barbara Piccoli Gamal Saadi Louise Fox Sharon Andreoli 陈樱花 Department of Clinical and Biological Sciences University of Torino Nephrology Centre Hospitalier Le Mans Department of Medicine Dubai Medical College National Clinical Research Center of Kidney Diseases Jinling Hospital Nanjing University School of Medicine Nephrology Moscow City Hospital n.a. S.P. Botkin Nephrology Moscow State University of Medicine and Dentistry Nephrology Russian Medical Academy of Continuous Professional Education the World Kidney Day Steering Committee Department of Medicine Division of NephrologyUniversity of British Columbia 国家肾脏疾病临床医学研究中心南京总医院

女性是现代家庭和社会的重要贡献者。但目前在世界范围内,男性和女性在受教育程度、医疗保健等方面仍存在显著差异。2018年第13个世界肾脏日恰逢国际妇女节,其主题是"肾脏和女性健康",给我们提供了一个关注女性健康,尤其是女性肾脏健康... 详细信息

女性是现代家庭和社会的重要贡献者。但目前在世界范围内,男性和女性在受教育程度、医疗保健等方面仍存在显著差异。2018年第13个世界肾脏日恰逢国际妇女节,其主题是"肾脏和女性健康",给我们提供了一个关注女性健康,尤其是女性肾脏健康的重要契机。为此,世界肾脏日指导委员会(World Kidney Day Steering Committee)特别组织了全球具有代表性的5位女性肾脏病学家,撰写了述评文章,借机呼吁肾脏病学术领域和全社会更加重视女性健康,让更多女性从肾脏病学术研究中获益。关注女性肾脏健康,利在当代功在千秋!

关键词：女性健康肾脏疾病世界范围肾脏病学学术研究国际妇女节现代家庭医疗保健

Essential Oils as an Alternative to Antibiotics to Reduce the Incidence and Severity of Necrotic Enteritis in Broiler Chickens: A Short Review

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Food and Nutrition Sciences 2023年第3期14卷 233-257页

作者： Makenly E. Coles Brittany D. Graham Juan D. Latorre Victor M. Petrone-garcia Xochitl Hernandez-Velasco Inkar Castellanos-Huerta Xiaolun Sun Billy M. Hargis Saeed El-Ashram Awad A. Shehata guillermo Tellez-Isaias Department of Poultry Science University of Arkansas Agricultural Experiment Station Fayetteville USA Facultad de Estudios Superiores Cuautitlan Universidad Nacional Autonoma de Mexico (UNAM) Cuautitlan Izcalli Estado de Mexico Mexico Departamento de Medicina y Zootecnia de Aves Facultad de Medicina Veterinaria y Zootecnia (FMVZ) UNAM Cd. de Mexico Mexico Programa de Maestría y Doctorado en Ciencias de la Producción y de la Salud Animal FMVZ UNAM Cd. de Mexico Mexico College of Life Science and Engineering Foshan University Foshan China Prophy-Institute for Applied Prophylaxis Bönen Germany

Due to the removal of antibiotic growth promoters (AGPs) and consumer pressure for antibiotic-free (ABF) or no antibiotics ever (NAE) poultry production, there is a need for sustainable alternatives to prevent disease in commercial poultry operations. Without AGPs, there has been a rise in diseases that were traditionally controlled by subtherapeutic levels of antibiotics in the diet. This has impacted the health of commercial poultry and has been a significant cost to poultry producers. To mitigate this, the industry has started to investigate alternatives to antibiotics to treat these forthcoming health issues, such as necrotic enteritis (NE). NE is an enteric disease caused by an over proliferation of toxigenic Clostridium perfringens (CP) in the gastrointestinal tract. Although CP is a commensal in the avian intestinal tract, dysbiosis caused by inflammation and impaired intestinal integrity facilitates uncontrolled replication of CP. Infectious agents, such as Eimeria maxima, appear to be a predominant predisposing factor that promotes NE. However, non-infectious stressors, including dietary changes, have also been associated with NE to some degree. As a result of increased pressure to restrict the use of antibiotics, there is a need for research evaluating the efficacy of alternatives, such as plant-derived essential oils, as potential tools to mitigate NE in commercial poultry flocks. The aim of this study is to review the effects of essential oils as an alternative to antibiotics to reduce the incidence and severity of necrotic enteritis in broiler chickens.

关键词： Necrotic Enteritis Essential Oils Chickens Clostridium perfringens Alternatives to Antibiotics

Clinical and prognostic implications of atrial fibrillation in patients undergoing transcatheter aortic valve implantation

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World Journal of Cardiology 2012年第1期4卷 8-14页

作者： Pablo Salinas Raúl Moreno Luis Calvo Santiago Jiménez-Valero guillermo Galeote Angel Sánchez-Recalde Teresa López-Fernández Sergio garcia-Blas Diego Iglesias Luis Riera Isidro Moreno-Gómez Jose María Mesa Ignacio Plaza Rocio Ayala Rosa Gonzalez José-Luis López-Sendón Division of Interventional Cardiology University Hospital La Paz Unit of Echocardiography University Hospital La Paz Department of Cardiology University Hospital La Paz Vascular Surgery University Hospital La Paz Department of Cardiac Anaesthesia University Hospital La Paz Cardiac Surgery University Hospital La Paz Department of Cardiology Hospital Infanta Sofia Department of Cardiology Hospital de la Cruz Roja

AIM: To study a cohort of consecutive patients under-going transcatheter aortic valve implantation (TAVI) and compare the outcomes of atrial fibrillation (AF) patients vs patients in sinus rhythm (SR). METHODS: All consecutive patients undergoing TAVI in our hospital were included. The AF group comprised patients in AF at the time of TAVI or with history of AF, and were compared with the SR group. Procedural, echocardiographic and follow-up variables were compared. Likewise, the CHA 2 DS 2-VASC stroke risk score and HAS-BLED bleeding risk score and antithrombotic treatment at discharge in AF patients were compared with that in SR patients. RESULTS: From a total of 34 patients undergoing TAVI, 17 (50%) were allocated to the AF group, of whom 15 (88%) were under chronic oral anticoagulation. Patients in the AF group were similar to those in the SR group except for a trend (P = 0.07) for a higher logistic EuroSCORE (28% vs 19%), and a higher prevalence of hypertension (82% vs 53%) and chronic renal failure (17% vs 0%). Risk of both stroke and bleeding was high in the AF group (mean CHA 2 DS 2-VASC 4.3, mean HAS-BLED 2.9). In the AF group, treatment at discharge included chronic oral anticoagulation in all except one case, and in association with an antiplatelet drug in 57% of patients. During a mean follow-up of 11 mo (maximum 32), there were only two strokes, none of them during the peri-procedural period: one in the AF group at 30 mo and one in the SR group at 3 mo. There were no statistical differences in procedural success, and clinical outcome (survival at 1 year 81% vs 74% in AF and SR groups, respectively, P = NS). CONCLUSION: Patients in AF undergoing TAVI show a trend to a higher surgical risk. However, in our cohort, patients in AF did not have a higher stroke rate compared to the SR group, and the prognosis was similar in both groups.

关键词： Aortic stenosis Transcatheter aortic valve implantation Stroke Atrial fibrillation