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Discrete-time Indefinite Stochastic linear Quadratic Optimal Control with Equality Constraints

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Discrete-time Indefinite Stochastic Linear Quadratic Optimal...

Transformation of coccolithophorid Emiliania huxleyi harboring a marine virus(Coccolithoviruses)serine palmitoyltransferase(SPT)gene by electroporation

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作者： guiling li Weihai Zhang College of Information Science and Engineering Shandong University of Science and Technology College of Information and Electrical Engineering Shandong University of Science and Technology

ISBN: (纸本)9781467355339

This paper studies the discrete-time indefinite stochastic linear quadratic optimal control problem with the equality terminal state constraints,which can be transformed into a mathematical programming with equality constraints. By means of matrix Lagrange theorem, a necessary condition for the existence of optimal linear state feedback control is given. The previous results on discrete-time stochastic linear quadratic optimal control without constraints, can be viewed as corollaries of the main theorem of this paper.

关键词： Discrete-time indefinite LQ control equality constraints matrix Lagrange theorem generalized differential Riccati equation

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Journal of Oceanology and limnology 2021年第2期39卷 693-704页

作者： Weicong CAI Xueting WANG Jinjing SU Jian li Jun ZENG guiling li Jingwen liU College of Food and Bioengineering Jimei UniversityXiamen 361021China Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering Xiamen 361021China

Emiliania huxleyi is the most prominent modern coccolithophore,a group of marine unicellular eukaryotes that play a critical role in ocean *** are large double stranded DNA viruses,which is responsible for the demise of large oceanic blooms formed by *** virus(EhVs)acquired a series of enzyme-coding genes predicted to be involved in the sphingolipid biosynthesis by horizontal gene transfer between ***,there is limited experimental validation identifying the functions of these genes in *** transformation of eukaryotic cells is a powerful tool to get an insight into gene functions of the studied *** palmitoyltransferase(SPT)catalyzes the first committed step in de novo sphingolipid biosynthetic ***,a novel vector system for the transformation of *** was *** contained fragments of promoter and terminator sequences of *** endogenic fucoxanthin chlorophyll a/c-binding protein gene“fcp”and harbored EhV-99B1 spt *** resultant recombinant transformation vectors pEhux-I-spt and pEhux-II were co-transferred into *** BOF92 by *** were obtained upon glufosinate-ammonium selection,and confirmed by Southern hybridization,genome PCR,qRT-PCR and Western blot screening of spt gene,which indicated that spt gene was integrated into the nuclear genome and was expressed at the mRNA and protein *** expression of the viral spt gene led to differences in lipid compositions analyzed using thin-layer chromatography(TLC).The results present the genetic transformation system for ***,providing additional genetic resource with potential for exploring basic biological questions such as the virus-host interactions.

关键词： E miliania huxleyi coccolithovirus genetic transformation serine palmitoyltransferase(SPT) total lipid

Characterization of the sphingolipid profiling of Emiliania huxleyi against virus infection

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Journal of Oceanology and limnology 2023年第4期41卷 1547-1557页

作者： Jingwen liU Jingjing GAO Enquan ZHANG Hanrui JIANG guiling li Jian li Jun ZENG Daren WU College of Ocean Food and Biological Engineering Jimei UniversityXiamen 361021China

lipidomics approach by UPLC-Q-Exactive-MS was used for the identification,quantification,comparison,and characterization of sphingolipids in virus infected marine Emiliania huxleyi BOF92 *** results show that 16 significantly changed sphingolipids(including Cer,CerG1,and SPHm)were identified during viral *** data confirmed previously recognized facts that viral infection led to a shift toward virus-specific sphingolipids,which is consistent with the down-regulation of genes involved in the host de novo sphingolipid ***,we revealed the upregulation of virusencoded homologous genes participating in de novo sphingolipids biosynthesis and virus-specific hydroxylated long chain bases(LCBs)as phytoCer,suggesting the competitive inhibition of host sphingolipid synthesis to produce the required building blocks for viral production,replication,and ***,Cer 40꞉1;2,Cer 40꞉2;2 isomer,and CerG139꞉0;2,Cer 39꞉0;2 as novel metabolite markers might indicate the general dysfunctions in *** in response to viral *** results show that viral infection led to a profound remodeling of host sphingolipidome,by which viruses depend on the hijacking of host sphingolipid metabolism to support the viral life cycle.

关键词： Emiliania huxleyi Coccolithovirus UPLC-Q-Exactive-MS sphingolipid metabolism biomarkers

Russia's Development Strategy

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Contemporary International Relations 2006年第4期16卷 1-16页

作者： Jiang li liu guiling li Dong

A complex process characterizes the evolvement of Russia’s develop-ment strategy which has drawn world-wide attention. In the paper, agreat length is given to President Putin’s strategy aimed at promotingeconomic re... 详细信息

关键词：俄罗斯发展战略民主政治经济发展

Pollination Biology of the Endangered Herbal Medicines Dendrobium chrysotoxum(Orchidaceae)

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Phyton-International Journal of Experimental Botany 2023年第7期92卷 1975-1986页

作者： Kai Luo Yanbing Bai Yan Jiang guiling li Min Jia Yongguo He Zhijun Yao Ziwei Xiao Xiaoqiong liu Wenjie liu Shanshan Yin Zesheng li Medicinal Plant Research Center Dehong Tropical Agriculture Research Institute of YunnanRuili678600China Human Resources Management Office The People’s Hospital of XiangyunXiangyun672100China

Pollination biology studies of the endangered herbal medicines Dendrobium chrysotoxum were conducted in natural pollination conditions using flower observation,pollinator observation and artificial pollination *** of *** with fragrance and nectar were pollinated by Ctenoplectra davidi Valhalla(Hymenoptera:Apidae)*** floral structure of *** adapted precisely to its *** had a low capsule setting(0.17%)under natural ***,compared to open pollination,artificial pollination experiments showed a significant increase in capsule setting,and *** was cross-compatible and self-compatible,but there was pollinator limitation *** study will provide important information for the preservation of this endangered species.

关键词： Breeding system Dendrobium chrysotoxum pollination pollination biology

Clinical Diagnosis and Treatment of Minimal Deviation Adenocarcinoma of the Cervix： a Report of Thirtheen Cases

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中国药理通讯 2013年第4期30卷 28-29页

作者： Yingying Lu guiling li Congjian Xu Obstetrics and Gynecology Hospital of Fudan University Shanghai 200000 China

Expression and significance of NRP-1 in xenografts of lung cancer cell in nude mouse

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The Chinese-German Journal of Clinical Oncology 2007年第3期6卷 254-257页

作者： Gang Wu Shuiying Guo guiling li Cancer Center of Union Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhan 430023 China Department of Oncology Xuhui District Center Hospital Shanghai 200031 China

Objective： To establish angiogenesis model of xenografts of lung cancer cell in nude mouse and investigate the expression of the neuropilin-1 （NRP-1） protein in tumors and its role in progression and angiogenesis of lung cancer. Methods： Human lung adenocarcinoma cells A549 were analyzed for the expression of vascular endothelial growth factor- 165 （VEGF16s） mRNA using RT-PCR in vitro. Two groups of nude mice were subcutaneously inoculated with A549 at different tumor-loading time. Two groups of xenografts were identified by hematoxylin and eosin （HE） staining, their microvessel density （MVD） were analyzed meanwhile. Two groups were analyzed for the expression of NRP-1 protein and their mean absorbency by using immunohistochemistry and automatic image analysis system respectively. Results： A549 expressed VEGF165 mRNA, and xenografts of A549 in nude mice were successfully established and confirmed by HE staining. The atypia of cancer cells and angiogenesis were occurred in two groups. Two groups of MVD were 13.06 ± 1.58, 23.61 ± 3.11 （vessels/mm^2）（P 〈 0.01）. NRP-1 protein was expressed in cytoplasm of vascular endothelium cells and partial tumor cells. Two groups of mean absorbency of NRP-1 were 0.1095 ± 0.0228, 0.1784 ± 0.0151 （P 〈 0.01）. Conclusion： The angiogenesis models of xenografts in nude mice with lung cancer cell A549 expressing VEGF165 mRNA at different tumor-loading times were established successfully. The expression of NRP-1 protein and MVD were increased with the tumor progression. Our results demonstrate that NRP-1 protein in tung cancer is related to angiogenesis.

关键词： lung cancer angiogenesis NRP-1

Clinical analysis on the analgesic effect of Methyl Carboprost and Diclofenac Sodium for intracavitary brachytherapy

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The Chinese-German Journal of Clinical Oncology 2007年第5期6卷 497-499页

作者： guiling li Yingqiu Song Fang Zhu Tingting Zhang The Cancer Center of Union Hospital Tongji Medical College Huazhong University of Science and TechnologyWuhan 430023 China

Objective:To observe the effects of Methyl Carboprost and Diclofenac Sodium on opening orifice of uterus and pain controlling in patients with uterine cervix cancer (UCC) when receiving intracavitary brachytherapy. Methods: Sixty patients with UCC of stage IIA-IIIB were divided into three groups randomly before receiving the intracavitary brachytherapy: the patients in group A received Methyl Carboprost in the hind fornix of the vagina, group B received Diclofenac Sodium in the anus, while group C was the control group. Results: The painlessness rates in groups A, B and C were 89.9%, 91.3% and 36.4%, respectively. The incidences of patients with relaxed uterus cervix in groups A, B and C were 91.7%, 85.9% and 48.9%, respectively. Conclusion: Methyl Carboprost and Diclofenac Sodium are useful in relaxing uterus cervix and pain controlling in patients with UCC when receiving intracavitary brachytherapy.

关键词： intracavitary brachytherapy Methyl Carboprost Diclofenac Sodium

基于增维精细积分法的单层垃圾体气体迁移分析

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环境科学学报 2010年第11期30卷 2236-2241页

作者：梅甫良李桂苓嘉兴学院土木工程研究所嘉兴314001

采用增维精细积分法对含覆盖层单层垃圾体一维气体迁移进行了瞬态分析.首先,对含覆盖层单层垃圾体的一维气体迁移控制方程进行有限差分离散,得到了其有限差分状态方程;然后,采用增维精细积分法建立了求解任一时刻垃圾体气压力的递推公式... 详细信息

采用增维精细积分法对含覆盖层单层垃圾体一维气体迁移进行了瞬态分析.首先,对含覆盖层单层垃圾体的一维气体迁移控制方程进行有限差分离散,得到了其有限差分状态方程;然后,采用增维精细积分法建立了求解任一时刻垃圾体气压力的递推公式.计算结果表明:本文提出的增维精细积分解与校正-预测解吻合得相当好,而且受时间步长影响相对较小.

关键词：垃圾填埋场气体迁移增维精细积分法