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Fluorescent immunosensor based on fluorescence resonance energy transfer between CdSe/ZnS quantum dots and Au nanorods for PRRSV detection

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Progress in Natural Science:Materials International 2022年第2期32卷 157-162页

作者： Xue Li Jinhua Li Wentao yang Dandan Han Na Yao Hongbin Zhao Xueying Chu Xuemei Liang Chunguang Bi Chunfeng Wang guilian yang College of Information Technology Jilin Agricultural University Nanophotonics and Biophotonics Key Laboratory of Jilin Province Changchun University of Science and Technology Jilin Provincial Engineering Research Center of Animal Probiotics Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding Jilin Agricultural University Changchun Huili Biotech Co. Ltd. Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development College of Life Sciences Jilin Agricultural University State Key Laboratory of Advanced Materials for Smart Sensing General Research Institute for Nonferrous Metals

An ultrasensitive and selective sandwich-type fluorescent immunosensor based on fluorescence resonance energy transfer(FRET) between CdSe/ZnS quantum dots(QDs) and Au nanorods(AuNRs) was developed for the rapid detection of porcine reproductive and respiratory syndrome virus(PRRSV).Modified CdSe/ZnS QDs and AuNRs were bioconjugated with primary antibodies M and GP5,*** the target antigen was present,a well-known sandwich-type form CdSe/ZnS QDs-M antibody-antigen-GP5 antibody-AuNRs was produced,and thus reducing the distance between the QDs and *** a result,FRET occurred and the fluorescence intensity of CdSe/ZnS QDs decreased,and this decrease was used to determine the antigen *** detection of PRRSV at low concentrations in swine serum was achieved with a limit of detection of 0.55 TCID50/mL and a linear detection range of 10~1-3.5 × 10~4 TCID50/***,the fluorescent immunosensor is selective and highly sensitive;specifically,it can detect PRRSV at low concentrations in swine serum over a large concentration *** proposed detection method can facilitate the development of high-performance fluorescent immunosensors for the detection of PRRSV and other antigens.

关键词： Fluorescence resonance energy transfer Immunosensor CdSe/ZnS quantum Dots Au nanorods Porcine reproductive and respiratory syndrome virus

African swine fever virus MGF505-3R inhibits cGAS-STING-mediated IFN-βpathway activation by degrading TBK1

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Animal Diseases 2022年第3期2卷 154-164页

作者： Mingyang Cheng Jiawei Luo Yuetong Duan Yu yang Chunwei Shi Yu Sun Yiyuan Lu Junhong Wang Xiaoxu Li Jianzhong Wang Nan Wang Wentao yang Yanlong Jiang guilian yang Yan Zeng Chunfeng Wang Xin Cao College of Veterinary Medicine Jilin Agricultural UniversityChangchunChina Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding Jilin Agricultural UniversityChangchunChina Jilin Provincial Engineering Research Center of Animal Probiotics Jilin Agricultural UniversityChangchunChina Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education Jilin Agricultural UniversityChangchunChina

African swine fever virus(ASFV)is an important pathogen causing acute infectious disease in domestic pigs and wild boars that seriously endangers the global swine *** ASFV is structurally complex and encodes a large number of functional proteins,no effective vaccine has been developed to ***,dissecting the mechanisms of immune escape induced by ASFV proteins is crucial.A previous study showed that the ASFV-encoded protein is an important factor in host *** this study,we identified a negative regulator,MGF505-3R,that significantly downregulated cGAS/STING-and poly(dG:dC)-mediated IFN-βand interferon stimulation response element(ISRE)reporter activity and suppressed IFNB1 and IFIT2 mRNA *** addition,TBK1,IRF3 and IκBαphosphorylation levels were also ***,MGF505-3R interacted with cGAS/TBK1/IRF3 and targeted TBK1 for degradation,thereby disrupting the cGAS-STING-mediated IFN-βsignaling pathway,which appears to be highly correlated with *** MGF505-3R expression enhanced IFN-βand IL-1β*** together,our study revealed a negative regulatory mechanism involving the MGF505-3R-cGAS-STING axis and provided insights into an evasion strategy employed by ASFV that involves autophagy and innate signaling pathways.

关键词： African swine fever virus MGF505-3R cGAS/STING signaling pathway TBK1 Innate immunity

Protective effect of recombinant Lactobacillus plantarum against H_(2)O_(2)-induced oxidative stress in HUVEC cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2021年第5期22卷 348-365,I0001页

作者： Guan WANG Mingyue HAO Qiong LIU Yanlong JIANG Haibin HUANG guilian yang Chunfeng WANG College of Veterinary Medicine Jilin Provincial Engineering Research Center of Animal ProbioticsKey Laboratory of Animal Production and Product Quality Safety of Ministry of EducationJilin Agricultural UniversityChangchun 130118China College of Agronomy Jilin Agricultural UniversityChangchun 130118China

This study probed the protective effect of recombinant Lactobacillus plantarum against hydrogen peroxide(H_(2)O_(2))-induced oxidative stress in human umbilical vein endothelial cells(HUVECs).We constructed a new functional ***(NC8-pSIP409-alr-angiotensin-converting enzyme inhibitory peptide(ACEIP))with a double-gene-labeled non-resistant screen as an expression vector.A 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT)colorimetric assay was carried out to determine the cell viability of HUVEC cells following pretreatment with *** cytometry(FCM)was used to determine the apoptosis rate of HUVEC *** aspartate specific proteinase(caspase)-3/8/9 activity was also assayed and western blotting was used to determine protein expression of B-cell lymphoma 2(Bcl-2),Bcl-2-associated X protein(Bax),inducible nitric oxide synthase(iNOS),nicotinamide adenine dinucleotide phosphate oxidase 2(gp91phox),angiotensin II(AngII),and angiotensin-converting enzyme 2(ACE2),as well as corresponding indicators of oxidative stress,such as reactive oxygen species(ROS),mitochondrial membrane potential(MMP),malondialdehyde(MDA),and superoxide dismutase(SOD).NC8-pSIP409-alr-ACEIP attenuated H_(2)O_(2)-induced cell death,as determined by the MTT ***8-pSIP409-alr-ACEIP reduced apoptosis of HUVEC cells by *** addition,compared to the positive control,the oxidative stress index of the H_(2)O_(2)-induced HUVEC(Hy-HUVEC),which was pretreated by NC8-pSIP409-alr-ACEIP,iNOS,gp91phox,MDA,and ROS,was decreased obviously;SOD expression level was increased;caspase-3 or-9 was decreased,but caspase-8 did not change;Bcl-2/Bax ratio was increased;permeability changes of mitochondria were inhibited;and loss of transmembrane potential was *** of the hypertension-related protein(AngII protein)in HUVEC cells protected by NC8-pSIP409-alr-ACEIP decreased and expression of ACE2 protein *** plantarum results suggested that

关键词： Oxidative stress Apoptosis Human umbilical vein endothelial cell(HUVEC) Hydrogen peroxide(H_(2)O_(2))

Macroscopic on-site magnifier-based evaluation to estimate visible tissue core cut-off lengths using EUS-FNA with 22-gauge needles

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Chinese Medical Journal 2024年第4期137卷 493-495页

作者： Jialiang Huang guilian Cheng Wei Wu Liming Xu Longjiang Xu Duanmin Hu Department of Gastroenterology Second Affiliated Hospital of Soochow UniversitySuzhouJiangsu 215004China Department of Pathology Second Affiliated Hospital of Soochow UniversitySuzhouJiangsu 215004China

To the Editor:Endoscopic ultrasound-guided fine needle aspiration(EUS-FNA)has replaced other high-risk invasive methods and has become the primary method of tissue collection around the gastrointestinal tract.^([1])To further improve the effectiveness and safety of EUS-FNA,pathologists may perform rapid on-site cytology evaluation(ROSE),but this process requires the presence of *** on-site evaluation(MOSE)has gained attention as it resolve the shortage of pathologist resources.^([2])Endoscopists can inspect the tissue core in real time and assess the adequacy of the punctured *** this study,we conducted a single-center and single-arm exploratory study to explore the value of MOSE in assessing specimen quality during magnifier-based EUS-FNA and to confirm the cut-off length of visible tissue core(VTC)for EUS-FNA to help endosonographers independently predict the adequacy of punctured *** VTC was defined as a white or incarnadine tissue core obtained from focal lesions with a relatively complete histological structure.

关键词： EUS needle visible

Effects of organic fertilizers on organic carbon accumulation in alkalized saline soil and silage maize yield

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International Journal of Agricultural and Biological Engineering 2024年第2期17卷 159-168页

作者： Lidong Ji Lei Li Rui Wang Xing Xu Fengju Zhang guilian Mao Teng Wang School of Forestry and Prataculture Ningxia UniversityYinchuan750021China School of Agriculture Ningxia UniversityYinchuan750021China School of Ecological Environment Ningxia UniversityYinchuan 750021China School of Life Sciences Ningxia UniversityYinchuan 750021China

To understand the combined effect of organic and chemical fertilizers on soil carbon emissions and carbon balance of a farmland ecosystem,this study investigated the organic fertilizer nitrogen replacing different proportions of chemical fertilizer *** results showed that,compared to F_(100),the O_(15)F_(85) treatment increased the yield and net ecosystem productivity carbon sequestration of silage maize under mild,moderate,and severe salinization levels,as well as the contents of soil organic carbon,microbial carbon,and humin carbon,while reducing plant carbon *** O_(15)F_(85) treatment did not significantly increase soil carbon emissions(CEC),but O_(30)F_(70),O_(45)F_(55) and O_(100) treatments significantly increased *** soil carbon balance analysis showed that the farmland ecosystem was a“sink”for atmospheric CO_(2) under each *** O_(15)F_(85) treatment produced an“excitation effect”to enhance the carbon sink effect of silage maize farmland under mild,moderate and severe salinization levels while maintaining stable production and *** the O_(100) treatment increased the carbon sink of farmland under different salinization levels,the yield was significantly reduced and did not represent practical production *** analysis showed that soil organic carbon components and ecosystem carbon balance were closely related to soil total salt,pH and bulk density,while soil dissolved organic carbon,humus carbon components and carbon emissions were closely related to soil moisture and ***,the purpose of improving the carbon sink of saline-alkali land can be achieved through soil salt inhibition,soil structure remodeling and water supplement and warming regulation,which provides technical and theoretical support for reducing carbon emissions,achieving carbon neutrality and alleviating global warming.

关键词： carbon balance carbon component organic carbon organic substitution saline-alkali land

Long-term performance of recycled asphalt mixtures containing high RAP and RAS

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Journal of Road Engineering 2024年第1期4卷 36-53页

作者： Jiangmiao Yu Zengyao Lin guilian Zou Huayang Yu Zhen Leng Yuan Zhang School of Civil Engineering and Transportation South China University of TechnologyGuangzhou 510641China Department of Civil and Environmental Engineering The Hong Kong Polytechnic UniversityHong KongChina State Key Laboratory of Subtropical Building and Urban Science South China University of TechnologyGuangzhou 510640China

The application of reclaimed asphalt pavement(RAP)and reclaimed asphalt shingles(RAS)on asphalt pavement can reduce the asphalt paving cost,conserve energy and protect the ***,the use of high contents of RAP and RAS in asphalt pavement may lead to durability issues,especially the fatigue cracking and thermal *** is necessary to conduct a series of analyses on asphalt mixtures containing high RAP and RAS,and seek methods to enhance their long-term *** paper provides a comprehensive over-view of the long-term performance of recycled asphalt mixtures containing high contents of RAP and *** findings in this research show that rutting resistance of high recycled asphalt mixtures is not a concern,whereas their resistance to fatigue and thermal cracking is not *** agents can be used to improve the thermal cracking resistance of high recycled asphalt *** optimum decision on recycling agents will improve the durability properties of high recycled asphalt *** is recommended that to use a balanced mixture design approach with testing of the blended asphalt binders will provide better understanding of long-term performance of recycled asphalt mixtures containing high RAP and RAS.

关键词： Recycled asphalt mixture Recycling agents Long-term performance Cracking

TaCHP encoding C1-domain protein stably enhances wheat yield in saline-alkaline fields

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Journal of Integrative Plant Biology 2024年第2期66卷 169-171页

作者： guilian Xiao Minqin Wang Xiaomeng Li Zhengning Jiang Hongjian Zhang Derong Gao Boqiao Zhang Guangmin Xia Mengcheng Wang The Key Laboratory of Plant Development andEnvironment Adaptation Biology Ministry of Education School of Life ScienceShandong UniversityQingdao 266237China Key Laboratory of Wheat Biology and GeneticImprovement for Low&Middle yangtze Valley Ministry ofAgriculture Lixiahe Agricultural Institute of Jiangsu ProvinceYangzhou 225007China

Salt and saline-alkaline stress seriously threaten crop production and food security worldwide(yang and Guo,2018).Identifying genes that confer tolerance to these conditions without yield penalty would help mitigate this problem through molecular breeding(Liang et al.,2023;Sahu and Liu,2023).Although numerous stress-tolerance genes have been identified,their application potential in molecular breeding has rarely been confirmed through multi-year and multi-site field trials.

关键词： breeding fir alkaline

Innate Cytokine Responses and Toll-Like Receptor Induced by Recombinant Porcine Rotavirus VP6 and VP7 Proteins Expressing in <i>Lactobacillus plantarum</i>NC8 Strain Colonization in Mice

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World Journal of Vaccines 2020年第1期10卷 17-31页

作者： Seria Masole Shonyela Wentao yang guilian yang Chunfeng Wang College of Animal Science and Technology Jilin University Changchun China Ministry of Agriculture Livestock and Fisheries Veterinary Complex Dar es Salaam Tanzania

The significant function of Toll-like receptors (TLR) is the detection of microbes by host guard cells that guide to the innate immune responses and to the successive adaptive. The current study patterns of TLR2, TLR3 and TLR9 expressing antigen presenting cells (APCs) in blood of mice after colonization with L. plantarum NC8 strain were assessed. The power of L. plantarum on serum innate cytokine and TLR responses stimulated by recombinant NC8-pSIP409-pgsA-VP6-DCpep, NC8-pSIP409-pgsA-VP7-DCpep and NC8-pSIP409-pgsA were also assessed. We confirmed that L. plantarum NC8 stimulated powerful TLR2 expressing APC responses in blood Recombinant strain stimulated a TLR3 response in spleen, and TLR9 responses were stimulated in blood or in spleen. Recombinant NC8-pSIP409-pgsA-VP6-DCpep, NC8-pSIP409-pgsA-VP7-DCpep on TLR2 and TLR9 expressing APC responses has a preservative outcome, reliable with the DCpep adjuvant outcome. In serum the recombinant NC8-pSIP409-pgsA-VP6-DCpep, NC8-pSIP409-pgsA-VP7-DCpep has increased the IL-4 and IFN-γ responses, except that on the TLR3 and TLR9 expressing CD14 APC responses it had an oppressive consequence in spleen and the IFN-α response in serum-stimulated by PRV. Our results give details that following PRV infection after immunization with NC8-pSIP409-pgsA-VP6-DCpep, NC8-pSIP409-pgsA-VP7-DCpep, the systemic TLR2, TLR3, and TLR9 expressing cDC and macrophage/monocyte responses.

关键词： Toll-Like Receptors Recombinant Porcine Rotavirus Lactobacillus plantaram Mice

Current Status of Prevalence, Possible Control and Risk Factors Associated with Porcine Cysticercosis from Endemic Countries in Africa

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World Journal of Vaccines 2018年第3期8卷 53-80页

作者： Seria Masole Shonyela guilian yang Chunfeng Wang College of Animal Science and Technology Jilin Provincial Engineering Research Center of Animal Probiotics Jilin Agricultural University Changchun China Ministry of Agriculture Livestock and Fisheries Dar es Salaam Tanzania

Porcine cysticercosis (PC) caused by Taenia solium larvae is continuing being an important zoonotic neglected disease in many developing countries, is responsible for severe health disorders such as seizures and death in human and it poses a serious public health risk. In general estimated prevalence for porcine cysticercosis by Ag-ELISA was 29.7% (95% CI [4.8 - 58.2]), by EITB was 24.7% (95% CI [9.2 - 38.2]), by Tongue examination was 9.4% (95% CI [0.2 - 13.2]), and by postmortem examination was 15% (95% CI [0.2 - 27.4]). Average seroprevalence of human cysticercosis by circulating antibody detection (Ab-ELISA) was ranged from 1.3% to 45.3%. Average seroprevalence of human cysticercosis by circulating T. solium antigen detection (Ag-ELISA) was ranged from 4.6% to 11.9%. Average seroprevalence of human cysticercosis by EITB was ranged from 6.9% to 16.7%. Average prevalence of human cysticercosis by CT scan was ranged from 23.2% to 54.6%. A fundamental factor in the occurrence of infections in most of the article was lack of health education to the community on T. solium, cysticercosis and taeniasis complex as the key towards control and eradication. The major causal factors of the disease occurrence were free roaming pigs and poor sanitary conditions.

关键词： Taenia solium Cysticercosis Prevalence Risk Factors Pig Management Africa