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Virulence Factor Profile of Staphylococcus aureus Isolated from Bovine Milk from Brazil

Food and Nutrition Sciences 2014年第15期5卷 1496-1505页

作者： Verena M.Santos Hellen B.martins Izadora S.Rezende Maysa S.Barbosa Ewerton F.Andrade Simone G.Souza guilherme B.Campos Pollianna S.Oliveira Daniel S.Sousa Danilo C.C.Da Silva Aline T.Amorim Jorge Timenetsky Mariluze P.Cruz Regiane Yatsuda Lucas M.Marques Instituto Multidisciplinar em Saúde Núcleo de Tecnologia em SaúdeUniversidade Federal da BahiaVitoria da ConquistaBrazil Instituto de Ciências Biomédicas Departamento de MicrobiologiaUniversidade de Sao PauloSao PauloBrazil

This study investigates the biofilm formation, presence and distribution of virulence genes and the capacity to induce an inflammatory response in strains of Staphylococcus aureus isolated from milk samples in Bahia, Brazil. A total of 132 samples of raw milk were collected from four dairy farms (designated A to D) located in southwestern Bahia, in the municipality of Vitória da Conquista, from October/2009 to September/2010. After processing of the samples, 94 (71.2%) S. aureus isolates were obtained. These strains were subjected to the antibiogram method MIC (Minimum Inhibitory Concentration). As for the pathogenicity, tests were performedin vitrobiofilm formation induced by glucose. Moreover, we performed PCR for their virulence genes: sea (enterotoxin A), seb (B), sec (C), pvl (Panton-Valentine Leukocidin), clfA (Clumping Factor A) and spa (protein A) and analysis of cytokine induction in the inflammatory response of J774 macrophages by exocellular lipoteichoic acid. No isolates were resistant to oxacillin and vancomycin. In biofilm production, 5.31% (5/94) isolates did not produce biofilm, 5.31% (5/94) of the samples were poor producers, 15.96% (15/94) strains were moderate producers, 18.09% (17/94) were producers and 55.32% (55/94) of isolates were strong biofilm producers. One (1.06%) isolate expressed the seb gene, one (1.06%) sec, 18 (19.2%) cflA and 44 (46.8%) had spa. There was no expression of sea and pvl between isolates analyzed. The analysis of cytokine induction in the inflammatory response did not show statistical difference in the levels of IL-6, TNF-α and IL-10 induction. However, there was statistical difference in IL-1 induction between isolates from different farms. Thus, it appears that the results obtained in this study show significant effects for the region studied, since it is an important dairy region, hence the need for further studies, with the intent of attracting funding that contributes to improving prevention and control in both dai

关键词： Milk Staphylococcus aureus Resistance Virulence Factors

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Sepsis after Administering an Intramuscular Iron Injection in the Arm of an Elder Patient with Multiple Comorbidities: A Case Report

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Journal of Biosciences and Medicines 2023年第5期11卷 158-165页

作者： Michele Paula dos Santos Taís Michele Werle Mariana martins Dantas Santos Carolina Boeira Soares Carlos Augusto Treviso Italo Trois Maestri Stella Duarte Pinto Carolina Hack Alves Laura Gomes Boabaid de Barros Maria Eduarda Perobelli Buzetto guilherme Boeira Soares Gabriel Lorentz Trein Aline Porto Bárbara Adelmann de Lima Fernanda Bercht Merten Luane Pereira Gomes Rafaela Siviero Caron-Lienert Miriam Viviane Baron School of Medicine Pontifical Catholic University of Rio Grande do Sul (PUCRS) Porto Alegre Brazil Course of Medicine Lutheran University of Brazil (ULBRA) Canoas Brazil School of Medicine and Health Sciences Pontifical Catholic University of Rio Grande do Sul (PUCRS) Porto Alegre Brazil Interdisciplinary Institute of Science and Health Education Porto Alegre Brazil

Background: Skin reactions and musculoskeletal injuries caused by intramuscular injection are an increased risk for skin and soft tissue infections and may culminate in sepsis. Objective: To describe the history of an elderly patient who developed a lesion in her right arm after home administration of intramuscular iron injection in this arm and its outcome. Methods: This is a case report of a patient who participated in a study in an intensive care unit. Results: This study describes a 65-year-old patient with multiple comorbidities who was admitted to the hospital complaining of pain for two months and difficulty in moving her right shoulder associated with skin and soft tissue infections in her right arm after intramuscular iron administration by a relative for the treatment of multifactorial anemia and refractory melena. The patient worsened her general condition and was transferred to the intensive care unit. Despite the therapy instituted, the patient developed sepsis of cutaneous origin, multiple organ dysfunction, and death. Conclusions: The administration of intramuscular medication requires the participation of qualified professionals, such as nurses, mainly within the scope of the Brazilian public health system which commonly comprises patients in a context of vulnerability.

关键词： Critical Care Education Continuing Injectables Septicemia Soft Tissue Infections Suppuration

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Water Quality Assessment of a Trout Farming Effluent,Bocaina,Brazil

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Journal of Water Resource and Protection 2014年第10期6卷 909-915页

作者： Bernardo Pinto Caramel Munique de Almeida Bispo Moraes Clovis Ferreira do Carmo Andre martins Vaz-dos-Santos Yara Aiko Tabata Joao Alexandre Saviolo Osti Carlos Massatoshi Ishikawa Marcos Aureliano Silva Cerqueira Cacilda Thais Janson Mercante Water Resources Center Fisheries InstituteSao PauloBrazil Federal University of Parana CuritibaBrazil Experimental Station of Salmon Sao PauloBrazil

The concern in mitigating the negative impact generated by the discharge of nutrients in the receiving water body is a challenge for the sustainable development of Brazilian fish farms. Thus, the purpose of this study was to evaluate the water quality and environmental impact caused by trout farming system effluent with focus on discharge of phosphorus. Sampling was performed on a weekly basis in triplicate from September to November 2010. Sample sites were distributed according to the water flow: upstream from trout farming system, water supply, effluent, artificial wetland, mixing zone and downstream (60 m from effluent). In the field, pH, conductivity, dissolved oxygen, water temperature and turbidity were measured. In laboratory, nitrogen and phosphorus series, chlorophyll a, total solids suspended and their organic and inorganic fractions were analyzed. For the good growth of trout in production system, the abiotic factors described in the water quality monitoring demonstrated acceptable values. Environmentally, after passing through the production system and artificial wetland, there was an increase in concentrations of total phosphorus, total nitrogen, orthophosphate, ammonium, chlorophyll a, total solids suspended and their organic and inorganic fractions (P < 0.05). These results are related with the quality of feed, feeding management and the inefficiency of the artificial wetland. Therefore, it is necessary to use best quality feed to meet the nutritional requirements of trout, maintaining an optimal feed conversion and reducing pollution generated by effluent.

关键词： Management Feeding Water Flow Discharge Phosphorus Pollution

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Perturbations in the uterine luminal fluid composition are detrimental to pregnancy establishment in cattle.

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畜牧与生物技术杂志(英文版) 2019年第1期

作者： Thiago martins guilherme Pugliesi Mariana Sponchiado AngelaM.Gonella-Diaza Oscar A.Ojeda-Rojas Frederich D.Rodriguez Roney S.Ramos Andrea C.Basso Mario Binelli Department of Animal Reproduction，School of Veterinary Medicine and Animal Science，University of S?o Paulo Department of Nutrition and Animal Production，School of Veterinary Medicine and Animal Science，University of S?o Paulo University Corporation of Huila ABS Global In Vitro Brasil Department of Animal Sciences，University of Florida

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