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Interferon-gamma inhibits aldehyde dehydrogenase^(bright)cancer stem cells in the 4T1 mouse model of breast cancer

Chinese Medical Journal 2022年第2期135卷 194-204页

作者： Xiufen Zhuang Guilan Shi Xiao Hu Huiru Wang Wen Sun Yanhong Wu Department of Oncology Affiliated Hospital of Jiangsu UniversityZhenjiangJiangsu 212000China Department of Immunology Zibo Vocational Institute Health SchoolZiboShandong 255000China Department of Medical Engineering Morsani College of MedicineTampaFL 33612USA Department of Oncology Suqian First HospitalSuqianJiangsu 223800China Department of Blood Transfusion The First Affiliated Hospital of USTCHefeiAnhui 230001China Department of Medical Microbiology and Immunology School of Basic Medical SciencesWannan Medical CollegeWuhuAnhui 241002China.

Background:Despite improvements in disease diagnosis,treatment,and prognosis,breast cancer is still a leading cause of cancer death for women.Compelling evidence suggests that targeting cancer stem cells(CSCs)have a crucial impact on overcoming the current shortcomings of chemotherapy and radiotherapy.In the present study,we aimed to study the effects of T cells and a critical anti-tumor cytokine,interferon-gamma(IFN-γ),on breast cancer stem cells.Methods:BALB/c mice and BALB/c nude mice were subcutaneously injected with 4T1 tumor cells.Tumor growth and pulmonary metastasis were assessed.ALDEFLOUR™assays were performed to identify aldehyde dehydrogenase^(bright)(ALDH^(br))tumor cells.ALDH^(br)cells as well as T cells from tumor-bearing BALB/c mice were analyzed using flow cytometry.The effects of CD8^(+)T cells on ALDH^(br)tumor cells were assessed in vitro and in vivo.The expression profiles of ALDH^(br)and ALDH^(dim)4T1 tumor cells were determined.The levels of plasma IFN-γwere measured by enzyme-linked immunosorbent assay,and their associations with the percentages of ALDH^(br)tumor cells were evaluated.The effects of IFN-γon ALDH expression and the malignancy of 4T1 tumor cells were analyzed in vitro.Results:There were fewer metastatic nodules in tumor-bearing BALB/c mice than those in tumor-bearing BALB/c nude mice(25.40 vs.54.67,P<0.050).CD8^(+)T cells decreased the percentages of ALDH^(br)4T1 tumor cells in vitro(control vs.effector to target ratio of 1:1,10.15%vs.5.76%,P<0.050)and in vivo(control vs.CD8^(+)T cell depletion,10.15%vs.21.75%,P<0.001).The functions of upregulated genes in ALDH^(br)4T1 tumor cells were enriched in the pathway of response to IFN-γ.The levels of plasma IFN-γdecreased gradually in tumor-bearing BALB/c mice,while the percentages of ALDH^(br)tumor cells in primary tumors increased.IFN-γat a concentration of 26.68 ng/mL decreased the percentages of ALDH^(br)4T1 tumor cells(22.88%vs.9.88%,P<0.050)and the protein levels of aldehyde dehydrogenase 1 family member A1 in 4T1 tumor cells(0

关键词： Cancer stem cells Aldehyde dehydrogenase Interferon-gamma CD8+T cells

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Correlation between Neutrophil Percentage-to-albumin Ratio and Coronary Heart Disease Complicated with Diabetes Mellitus

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Medicinal Plant 2024年第2期15卷 43-47页

作者： Wenjing ZHANG Yuhong shi guilan ZHANG Minghui ZHANG Zhoukai CUI Jiaying WANG Anjun ZHU Pingquan County Hospital Pingquan 067500China

[Objectives]This study was conducted to explore the relationship between neutrophil percentage-to-albumin ratio(NPAR)and coronary heart disease complicated with diabetes.[Methods]A total of 603 patients with coronary heart disease who underwent coronary angiography in Pingquan County Hospital from January,2023 to December,2023 and met the inclusion criteria were included as the research object.All the patients were divided into a coronary heart disease complicated with diabetes group(CAD+T2DM group)(n=298 cases)and a control group(CAD group)(n=305 cases),according to patients medical history,heart color ultrasound and biochemical test results.The clinical data,biochemical test results and coronary artery imaging data of patients were recorded,and the Gensini score was calculated.The neutrophil percentage(NEUT%)and albumin count were determined to calculate NPAR.[Results]The NPAR value of the coronary heart disease complicated with diabetes mellitus group was(1.6±0.42),which was significantly higher than that of the control group(1.47±0.49),and the difference was statistically significant(P<0.05).The area under the ROC curve was 0.619(95%CI:0.591-0.675,P<0.05),and the prediction of coronary heart disease complicated with diabetes using NPAR showed a Youden index of 0.31,a sensitivity of 60.4%,a specificity of 40.3%,and a best cut-off score of 1.4506.[Conclusions]The neutrophil percentage-to-albumin ratio(NPAR)is closely related to coronary heart disease complicated with diabetes mellitus,and NPAR has clinical application value in the diagnosis of coronary heart disease complicated with diabetes mellitus.

关键词： Neutrophil percentage-to-albumin ratio(NPAR) Gensini score Coronary heart disease Coronary angiography

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Synergistic action on hypnosia: Yinao capsules with pentobarbital sodium of threshold and sub-threshold dosages

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Neural Regeneration Research 2007年第2期2卷 91-94页

作者： guilan Zhang Mingsan Miao Jingjing shi Yalei Yang Henan College of Traditional Chinese Medicine Zhengzhou 450008 Henan Province China

BACKGROUND： Sedative and hypnotic drugs could cure insomnia in a dependent manner, and traditional Chinese medicine has some superiority in treating insomnia. OBJECTIVE： To observe the synergistic action of yinao capsules with pentobarbital sodium in threshold and sub-threshold dosages in hypnosia and sedation. DESIGN： A completely randomized grouping design and control experiment. SETTING： Pharmacological laboratory, College of Pharmacy, Henan College of Traditional Chinese Medicine. MATERIALS： Totally 200 grade II Kunming mice of 18 - 21 g, either male or female, were used. Yinao capsules, main ingredients of which were turtleback glue, thinleaf milkwort root, Chinese magnoliavine fruit, mythic fungus, tangshen, ginseng and grassleaf sweetflag rhizome, were offered by Chinese-American Huayi Pharmacy, Co.,Ltd. （ratified number： 040901）; Kangnaoshuai capsules, main ingredients of which were prepared rehmannia root, tuber fleeceflower root, ginseng, membranous milkvetch root, thinleaf milkwort root, Fushen, grassleaf sweetflag rhizome, spine date seed, lecithin, barbary wolfberry fruit, pueraria root, vitamin E, etc., were produced by shijiazhuang Siyao, Co.,Ltd. （ratified number： 040964）; Pentobarbital sodium was produced by China Medicine （Group） Shanghai Chemical Reagent, Co,.Ltd. （Ratified number： 030816）. ZZ-6 mice spontaneous activity apparatus was produced by Chengdu Taimeng Science and Technology, Co.,Ltd. METHODS： The experiment was carried out in the Animal Experimental Center, Henan College of Traditional Chinese Medicine from October to December in 2005. （1） Influence of Yinao capsules on the spontaneous activity of mice： Fifty mice were randomly divided into five groups with 10 mice in each group： Mice in the large, middle and small dosages of Yinao capsules groups were intragastrically infused with Yinao capsules suspension （1.36, 0.68, 0.34 g/kg）; Those in the Kangnaoshuai capsules group were infused with Kangnaoshuai suspension （1.12 g/kg）; Those in the control group were given physiologic saline o

关键词： REINFORCING AGENTS insomnia capsules pentobarbital drug synergism

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Application of a ddRT-PCR to quantify seasonal influenza virus for viral isolation

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Biosafety and Health 2022年第5期4卷 299-302页

作者： Yimeng Liu Jiachen Zhao Xiaomin Peng guilan Lu Weixian shi Zhaomin Feng Hui Xu Shujuan Cui Yang Pan Daitao Zhang Peng Yang Quanyi Wang Institute for Infectious Disease and Endemic Disease Control Beijing Center for Disease Prevention and ControlBeijing 100013China

Viral isolation in cell cultures has been regarded for decades as the“gold standard”for the laboratory diagnosis of influenza viral infections.Not all viral strains could be isolated from clinical samples.This study aimed to quantify the viral load in the samples before isolation to save working time and improve working efficiency.Four hundred samples from patients with influenza-like cases were confirmed pdmH1N1 positive(200 cases)and B Victoria(BV)positive(200 cases)by whole-genome sequencing and analyzed by ddPCR for viral load in samples before isolation,and isolation results were verified by hemagglutination(HA)assay and hemagglutination-inhibition(HI)tests.Probit regression analysis was used to calculate the isolation viral load limit with a 95%probability level by SPSS 19.0 software.The results showed that the isolation limit of viral load was 4.9×10^(4)(95%CI:2.5×10^(4)–9.0×10^(4))copies/mL for pdmH1N1 and 1.9×10^(4)(95%CI:7.8×103–3.6×10^(4))copies/mL for BV.The isolation rate of clinical samples is positively correlated with the viral load in clinical samples,which can be used for viral culture,providing important guidance for daily work.

关键词： Influenza virus ddRT-PCR Viral load Viral isolation

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控股股东特征与现金分红:来自中小板上市公司的经验证据

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财经科学 2015年第5期 55-65页

作者：李桂兰罗诗湖南农业大学商学院

本文通过比较中小企业板和主板A股2006—2012年控股股东相关数据,从最终层面探讨控股股东对中小板上市公司现金分红倾向与力度的影响,运用多元线性回归和Logistic回归进行面板数据验证,研究结果表明控股股东控制权、两权分离度和经济性... 详细信息

本文通过比较中小企业板和主板A股2006—2012年控股股东相关数据,从最终层面探讨控股股东对中小板上市公司现金分红倾向与力度的影响,运用多元线性回归和Logistic回归进行面板数据验证,研究结果表明控股股东控制权、两权分离度和经济性质对中小板上市公司现金股利分配有显著作用。

关键词：控股股东现金股利中小板

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KCNN2突变致儿童震颤性肌阵挛-肌张力障碍1例报道

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中华神经医学杂志 2024年第1期23卷 66-68页

作者：蔡淑英时艺黄种钦彭桂兰厦门市妇幼保健院/厦门大学附属妇女儿童医院儿童神经康复科厦门361003 厦门大学附属第一医院检验科厦门361003

肌阵挛-肌张力障碍(myoclonus-dystonia,M-D)是一种临床综合征,其特征是肌阵挛和肌张力障碍,累及颈部、躯干和上肢;同时伴有精神症状和酒精反应 [1]。既往一些类似于M-D的具有不同临床特征的家族表型已经被描述,并被归类为"M-D综合征"... 详细信息

肌阵挛-肌张力障碍(myoclonus-dystonia,M-D)是一种临床综合征,其特征是肌阵挛和肌张力障碍,累及颈部、躯干和上肢;同时伴有精神症状和酒精反应 [1]。既往一些类似于M-D的具有不同临床特征的家族表型已经被描述,并被归类为"M-D综合征"。表皮肌聚糖( SCGE)突变约占常染色体显性遗传的家族性M-D病例的1/3 [2]。 KCNN2变异所致的表型与经典的M-D表型相比,相同的特征包括书写痉挛、斜颈和手部姿势异常,不同的特征包括远端的肌阵挛、平衡问题,以及小脑眼征 [3]。因此, KCNN2可能是一种新的M-D综合征致病基因。厦门市妇幼保健院/厦门大学附属妇女儿童医院儿童神经康复科2023年5月收治1例由 KCNN2突变致震颤性M-D患儿,现回顾性分析其临床、电生理学和遗传学特点,并结合相关文献报道如下,以期提高临床医师对该病的认识。

关键词： KCNN2 基因突变震颤肌阵挛-肌张力障碍

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藿香正气系列制剂指纹图谱研究

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中国现代应用药学 2020年第14期37卷 1714-1718页

作者：罗廷顺石桂兰耿玲大理大学基础医学院云南大理671000 大理州食品药品检验所云南大理671000 大理州第二人民医院云南大理671000

目的建立藿香正气系列制剂统一的HPLC指纹图谱。方法采用HPLC,色谱柱为Waters Symmetry■C18(250 mm×4.6 mm,5μm),流动相为乙腈(A)-0.5%的冰醋酸水溶液(B)梯度洗脱,流速为1.0 mL·min^-1,检测波长为290 nm,柱温为40℃。结果初步建立... 详细信息

目的建立藿香正气系列制剂统一的HPLC指纹图谱。方法采用HPLC,色谱柱为Waters Symmetry■C18(250 mm×4.6 mm,5μm),流动相为乙腈(A)-0.5%的冰醋酸水溶液(B)梯度洗脱,流速为1.0 mL·min^-1,检测波长为290 nm,柱温为40℃。结果初步建立了藿香正气系列制剂HPLC指纹图谱共有模式,共标定了18个共有峰,通过对照品归属法,确定了橙皮苷、欧前胡素、和厚朴酚、异欧前胡素、厚朴酚和苍术素共6个化合物,13批藿香正气系列制剂相似度为0.802~0.999。结论本法分析时间短,重复性好,精密度高,可用于藿香正气系列制剂的质量控制。

关键词：藿香正气高效液相色谱指纹图谱

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Political History of Books:A Case Study of Precedents of Ritual Protocol for Jin's Imperial Ministers and Annotations to the Report on Jin's Official Posts

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Contemporary Social Sciences 2019年第2期4卷 66-83页

作者： Huang Zhen Zhu guilan School of History and Cultures Sichuan University 不详

This article takes Fu Chang's Precedents of Ritual Protocol for Jin's Imperial Ministers and Xun Chuo's Annotations to the Report on Jin's Official Posts as a means to observe the interactions between books and political processes during the Western and Eastern Jin dynasties. Both Fu Chang and Xun Chuo were employed as high-ranking officials, with a background of being from the Central Plain, by the regime of the Later Zhao founded by shi Le. Their purpose for writing the two books was to provide guidance for the regime's institutional establishment. The collapse of shi Le's regime led to the flow of people and their possessions to the south. During these movements, the two books along with other materials and records were brought to Jiankang and then contributed to the institutional and cultural development in the middle and late Eastern Jin Dynasty. As historical facts and references for political reformation, these books revealed the duality of institutional writings. The collection and dispersion of books after the Disaster of Yongjia, as well as the social and cultural changes, should be placed in a wider political process for further examination.

关键词： history of the book the Later Zhao founded by shi Le the Eastern Jin Dynasty institution institutional writing

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Virus-inspired surface-nanoengineered antimicrobial liposome:A potential system to simultaneously achieve high activity and selectivity

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Bioactive Materials 2021年第10期6卷 3207-3217页

作者： Yin shi Xiaoqian Feng Liming Lin Jing Wang Jiaying Chi Biyuan Wu Guilin Zhou Feiyuan Yu Qian Xu Daojun Liu guilan Quan Chao Lu Xin Pan Jianfeng Cai Chuanbin Wu College of Pharmacy Jinan UniversityGuangzhouGuangdong511443China Department of Pharmaceutics School of Pharmaceutical SciencesSun Yat-Sen UniversityGuangzhouGuangdong510006China Medical College Shantou UniversityShantouGuangdong15041China Department of Chemistry University of South FloridaTampaFL33620United States

Enveloped viruses such as SARS-CoV-2 frequently have a highly infectious nature and are considered effective natural delivery systems exhibiting high efficiency and specificity.Since simultaneously enhancing the activity and selectivity of lipopeptides is a seemingly unsolvable problem for conventional chemistry and pharmaceutical approaches,we present a biomimetic strategy to construct lipopeptide-based mimics of viral architectures and infections to enhance their antimicrobial efficacy while avoiding side effects.Herein,a surface-nanoengineered antimicrobial liposome(SNAL)is developed with the morphological features of enveloped viruses,including a moderate size range,lipid-based membrane structure,and highly lipopeptide-enriched bilayer surface.The SNAL possesses virus-like infection to bacterial cells,which can mediate high-efficiency and high-selectivity bacteria binding,rapidly attack and invade bacteria via plasma membrane fusion pathway,and induce a local“burst”release of lipopeptide to produce irreversible damage of cell membrane.Remarkably,viral mimics are effective against multiple pathogens with low minimum inhibitory concentrations(1.6-6.3μg mL􀀀1),high bactericidal efficiency of>99%within 2 h,>10-fold enhanced selectivity over free lipopeptide,99.8%reduction in skin MRSA load after a single treatment,and negligible toxicity.This bioinspired design has significant potential to enhance the therapeutic efficacy of lipopeptides and may create new opportunities for designing next-generation antimicrobials.

关键词： Virus-inspired mimics Antimicrobial lipopeptides Liposomes Virus-like infections Activity and selectivity

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LF冷态碳钢精炼渣循环再利用的试验研究

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炼钢 2014年第3期30卷 46-49页

作者：仪桂兰史永林山西太钢不锈钢股份有限公司技术中心山西太原030003

通过LF碳钢精炼渣压球试验,分析比较了使用几种粘结剂的压球理化性能,确定了较合理的压球方案,同时也进行了LF精炼渣压球代替预熔渣的工业化试验。并利用差热分析,分析了循环再利用的LF冷态碳钢精炼渣适宜采用的冷却方式,为实现LF精炼... 详细信息

通过LF碳钢精炼渣压球试验,分析比较了使用几种粘结剂的压球理化性能,确定了较合理的压球方案,同时也进行了LF精炼渣压球代替预熔渣的工业化试验。并利用差热分析,分析了循环再利用的LF冷态碳钢精炼渣适宜采用的冷却方式,为实现LF精炼渣代替预熔渣利用提供了技术支持。

关键词：循环再利用 LF碳钢精炼渣粘结剂压球

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